Penurunan Respon Nyeri Akut Pada Bayi Prematur Yang Dilakukan Prosedur Invasif Melalui Developmental Care

Pain is a stimulus that can damage the developing brains of premature babies. Developmental care is one strategyin reduce pain caused by invasive procedure. The objective of this research was to determine the effects of developmentalcare on acute pain response in premature babies who had been performed invasive procedure. This study used quasiexperimentresearch design non equivalen control group, before and after design, involving 42 sample consisted of 21premature babies in both control and intervention groups, technical sampling used accidental sampling. This studyfounded that there was a significant difference in change score of acute pain response between on intervention andcontrol groups (p= 0.000; α= 0.05), developmental care decreased 2,05 point of pain scores). Developmental care wasrecommended to be applied in nursing care for premature babies. Further research on effect developmental care ondevelopment of premature babies influence cognitive, languanges, and motor skills aspects need to be designed

Identification of main fungal disease from hydroponic melon in greenhouse

Hydroponic melon is a method of cultivating melons and grown in a greenhouse. The melon cultivation has problems, namely plant diseases, even in a closed greenhouse. The melon plant disease caused huge losses in melon cultivation until the melon plants died. The purpose of this descriptive study was to identify the cause of the melon plant disease. Samples of sick melon plants were taken and grown on PDA media in the laboratory. The fungus that grows is identified based on the literature. The identification results showed that the main disease causing hydroponic melon plants in the greenhouse was the fungus Fusarium oxysporum f.sp. melonis. The fungi have crescent-shaped macroconidia with 1-3 septa, hyaline, thick walls, and form chlamydospores that are spherical in intercalary and hyaline

The novel features of Plantago ovata seed mucilage accumulation, storage and release

Seed mucilage polysaccharide production, storage and release in Plantago ovata is strikingly different to that of the model plant Arabidopsis. We have used microscopy techniques to track the development of mucilage secretory cells and demonstrate that mature P. ovata seeds do not have an outer intact cell layer within which the polysaccharides surround internal columellae. Instead, dehydrated mucilage is spread in a thin homogenous layer over the entire seed surface and upon wetting expands directly outwards, away from the seed. Observing mucilage expansion in real time combined with compositional analysis allowed mucilage layer definition and the roles they play in mucilage release and architecture upon hydration to be explored. The first emergent layer of hydrated mucilage is rich in pectin, extremely hydrophilic, and forms an expansion front that functions to ‘jumpstart’ hydration and swelling of the second layer. This next layer, comprising the bulk of the expanded seed mucilage, is predominantly composed of heteroxylan and appears to provide much of the structural integrity. Our results indicate that the synthesis, deposition, desiccation, and final storage position of mucilage polysaccharides must be carefully orchestrated, although many of these processes are not yet fully defined and vary widely between myxospermous plant species.Jana L. Phan, James M. Cowley, Kylie A. Neumann, Lina Herliana, Lisa A. O’Donovan, Rachel A. Burto

A chromosome-level genome assembly of Plantago ovata

Plantago ovata is cultivated for production of its seed husk (psyllium). When wet, the husk transforms into a mucilage with properties suitable for pharmaceutical industries, utilised in supplements for controlling blood cholesterol levels, and food industries for making gluten-free products. There has been limited success in improving husk quantity and quality through breeding approaches, partly due to the lack of a reference genome. Here we constructed the frst chromosome-scale reference assembly of P. ovata using a combination of 5.98 million PacBio and 636.5 million Hi-C reads. We also used corrected PacBio reads to estimate genome size and transcripts to generate gene models. The fnal assembly covers ~ 500 Mb with 99.3% gene set completeness. A total of 97% of the sequences are anchored to four chromosomes with an N50 of~ 128.87 Mb. The P. ovata genome contains 61.90% repeats, where 40.04% are long terminal repeats. We identifed 41,820 protein-coding genes, 411 non-coding RNAs, 108 ribosomal RNAs, and 1295 transfer RNAs. This genome will provide a resource for plant breeding programs to, for example, reduce agronomic constraints such as seed shattering, increase psyllium yield and quality, and overcome crop disease susceptibility.Lina Herliana, Julian G. Schwerdt, Tycho R. Neumann, Anita Severn-Ellis, Jana L. Phan, James M. Cowley, Neil J. Shirley, Matthew R.Tucker, Tina Bianco, Miotto, Jacqueline Batley, Nathan S. Watson, Haigh, Rachel A. Burto

Analisis Faktor yang Berhubungan dengan Status Imunisasi Dasar Bayi 9-12 Bulan di Wilayah Kerja Puskesmas Sikumana Kota Kupang

Background. Immunization as a preventive way to prevent infectious diseases. Aim. This study aims to analyze the factors associated with infant basic immunization status (9-12 months) in the Work Area of Sikumana Health Center, Kupang City. Method. The design of this study was quantitative by using a cross sectional study design. Sampling in this study uses purposive sampling with a total sample of 116 respondents, this research instrument uses a questionnaire sheet. Results. The results of this study showed that the majority of respondents had the support of health workers by percentage (63.6%), distance to health facilities was less than 1 km by percentage (62.7%), mother's education (basic) by percentage (62.7%) , and most of the respondents 'knowledge of mothers are in the good category with a percentage (70.9%), occupational mothers who work with a percentage (63.6%), and the majority of respondents' attitudes or behaviors are in the poor category with a percentage (58.2% ) and infant immunization status is incomplete with a percentage (56.4%). Based on the Chi Square statistical test results found that there are 3 factors related to the basic immunization status of infants (9-12 months), namely maternal education with a result of p = 0.019 where p <0.05, maternal employment factors with a result of p = 0.062 where p <0, 05 and maternal behavior (attitude) with the result p = 0.075 where p <0.05. It is hoped that health workers pay more attention and increase the knowledge of mothers about infant immunization, and coordinate with Posyandu cadres related to the immunization schedule

A small-scale extraction pipeline for rapid analysis of seed mucilage characteristics

Background: Myxospermy is a process by which the external surfaces of seeds of many plant species produce mucilage—a polysaccharide-rich gel with numerous fundamental research and industrial applications. Due to its functional properties the mucilage can be difficult to remove from the seed and established methods for mucilage extraction are often incomplete, time-consuming and unnecessarily wasteful of precious seed stocks. Results: Here we tested the efficacy of several established protocols for seed mucilage extraction and then downsized and adapted the most effective elements into a rapid, small-scale extraction and analysis pipeline. Within 4 h, three chemically- and functionally-distinct mucilage fractions were obtained from myxospermous seeds. These fractions were used to study natural variation and demonstrate structure–function links, to screen for known mucilage quality markers in a field trial, and to identify research and industry-relevant lines from a large mutant population. Conclusion: The use of this pipeline allows rapid analysis of mucilage characteristics from diverse myxospermous germplasm which can contribute to fundamental research into mucilage production and properties, quality testing for industrial manufacturing, and progressing breeding efforts in myxospermous crops.James M. Cowley, Lina Herliana, Kylie A. Neumann, Silvano Ciani, Virna Cerne and Rachel A. Burto