The Botanical Record of Archaeobotany Italian Network - BRAIN: a cooperative network, database and website

The BRAIN (Botanical Records of Archaeobotany Italian Network) database and network was developed by the cooperation of archaeobotanists working on Italian archaeological sites. Examples of recent research including pollen or other plant remains in analytical and synthetic papers are reported as an exemplar reference list. This paper retraces the main steps of the creation of BRAIN, from the scientific need for the first research cooperation to the website which has a free online access since 2015

The Botanical Record of Archaeobotany Italian Network - BRAIN: a cooperative network, database and website

The BRAIN (Botanical Records of Archaeobotany Italian Network) database and network was developed by the cooperation of archaeobotanists working on Italian archaeological sites. Examples of recent research including pollen or other plant remains in analytical and synthetic papers are reported as an exemplar reference list. This paper retraces the main steps of the creation of BRAIN, from the scientific need for the first research cooperation to the website which has a free online access since 2015

The Botanical Record of Archaeobotany Italian Network - BRAIN: a cooperative network, database and website

Con autorización de la revista para autores CSIC[EN] The BRAIN (Botanical Records of Archaeobotany Italian Network) database and network was developed by the cooperation of archaeobotanists working on Italian archaeological sites. Examples of recent research including pollen or other plant remains in analytical and synthetic papers are reported as an exemplar reference list. This paper retraces the main steps of the creation of BRAIN, from the scientific need for the first research cooperation to the website which has a free online access since 2015.Peer reviewe

Comparison of Mobile Phone and CCD Cameras for Electrochemiluminescent Detection of Biogenic Amines

Biogenic amines are an important and widely studied class of molecules due to their link to the physiological processes of food-related illnesses and histamine poisoning. Electrochemiluminescent (ECL) detection offers an inexpensive and portable analytical method of detection for biogenic amines when coupled with recent advancements in low-cost carbon-based electrodes and a smartphone camera. In this work, a mobile phone camera was evaluated against a piece of conventional instrumentation, the charge-coupled device, for the detection of ECL from the reaction of biogenic amines with the luminescent compound tris(2,2′-bipyridyl)ruthenium(II). Assisted by a 3D-printed light-tight housing, the mobile phone achieved limits of detection of 127, 425 and 421 μM for spermidine, putrescine, and histamine, respectively. The mobile phone’s analytical figures of merit were lesser than the CCD camera but were still within the range to detect contamination. In an exploration of real-world samples, the mobile phone was able to determine the contents of amines in skim milk on par with that of a CCD camera

Trisomy 12 and elevated GLI1 and PTCH1 transcript levels are biomarkers for Hedgehog-inhibitor responsiveness in CLL

Immunobiology of allogeneic stem cell transplantation and immunotherapy of hematological disease

Trisomy 12 and elevated GLI1 and PTCH1 transcript levels are biomarkers for Hedgehog-inhibitor responsiveness in CLL

Hedgehog (HH) signaling is activated in various lymphoid malignancies, but conflicting results exist about its role in chronic lymphocytic leukemia (CLL). Here, we demonstrate that the expression of essential HH pathway components like GLI1, PTCH1, and the HH ligands is highly diverse in CLL. A subset of 36.7% of 60 tested CLL samples responded to all 3 SMOOTHENED (SMO) inhibitors, whereas 40% were completely resistant. Responsiveness correlated with elevated GLI1 and PTCH1 transcript levels and the presence of trisomy 12, whereas no other karyotype correlated with responsiveness. All trisomy 12 CLLs displayed constitutive HH pathway activation driven by autocrine DESERT HH (DHH) ligand secretion, which could be blocked by the HH-blocking Ab 5E1. Cocultures with DHH-expressing BM stromal cells reduced sensitivity of CLLs to SMO-inhibitor treatment by activation of noncanonical ERK phosphorylation directly downstream of the PTCH1 receptor without involvement of SMO and could be overcome by the HH-blocking Ab 5E1 or a combination of SMO and ERK inhibitors. Our results demonstrate that the HH-signaling pathway is an interesting therapeutic target for a subset of patients with CLL, characterized by high GLI1 and PTCH1 transcript levels, and all patients with trisomy 12 and indicate HH-blocking Abs to be favorable over SMO inhibitors in overcoming stroma-mediated protective effects

BRAIN \u2013 cooperative network and website

In 2015, a paper on the archaeobotany as a key tool \u2018for the understanding of the biocultural diversity of the Italian landscape\u2019 gave rise to a new initiative, the realization of the first cooperative network of archaeobotanists and palynologists working on archaeological sites located in Italy. The Botanical Record of Archaeobotany Italian Network has been introduced at MedPalyno2015 in Rome. Now the BRAIN database is ready as a website at https://brainplants.unimore.it The website (programmed by Matteo di Lena, and Federico Camerini) consists of 6 pages, two of which are dedicated to the database including "Sites" and "References". Site position and density are immediately visible in a map while three graphs show updated statistics on the number of sites per Region, or per Cultural period, or per type of Record studied (e.g., pollen, seeds or charcoals). References may be sorted in alphabetical order, or by specific (first) letter or name of authors. The first set of archaeological or human-related sites studied in Italy is available as an interactive sheet, and > than 1160 visits have been recorded in the first year of activity. The number of included sites is increasing, and the website is an user-friendly instrument, easy to navigate and to be implemented. The BRAIN network lies in this database which is a useful instrument for both the research of one single group or for joint researches that will be planned to produce synthesis. The website is also a good way to publicize the impressive work that has been carried out, and will continue, in the field of archaeobotany in the Italian on-sites (archaeological) or nearsites (human-related environmental sites)