Information developer-user system linking roles of education assistants in the Missouri small farm family program

"August 1982."Includes bibliographical references (pages 34-35)

Stable Isotopic Tracing—A Way Forward for Nanotechnology

Numerous publications and reports have expressed health and safety concerns about the production and use of nanoparticles, especially in areas of exposure monitoring, personal use, and environmental fate and transport. We suggest that stable isotopic tracers, which have been used widely in the earth sciences and in metabolic and other health-related studies for several decades, could be used to address many of these issues. One such example we are pursuing is the use of stable isotopes to monitor dermal absorption of zinc and titanium oxides in sunscreen preparations and other personal care products. Other potential applications of this tracing approach are discussed

Duration of patients’ visits to the hospital emergency department

Background Length of stay is an important indicator of quality of care in Emergency Departments (ED). This study explores the duration of patients’ visits to the ED for which they are treated and released (T&R). Methods Retrospective data analysis and multivariate regression analysis were conducted to investigate the duration of T&R ED visits. Duration for each visit was computed by taking the difference between admission and discharge times. The Healthcare Cost and Utilization Project (HCUP) State Emergency Department Databases (SEDD) for 2008 were used in the analysis. Results The mean duration of T&R ED visit was 195.7 minutes. The average duration of ED visits increased from 8 a.m. until noon, then decreased until midnight at which we observed an approximately 70-minute spike in average duration. We found a substantial difference in mean duration of ED visits (over 90 minutes) between Mondays and other weekdays during the transition time from the evening of the day before to the early morning hours. Black / African American patients had a 21.4-minute longer mean duration of visits compared to white patients. The mean duration of visits at teaching hospitals was substantially longer than at non-teaching hospitals (243.8 versus 175.6 minutes). Hospitals with large bed size were associated with longer duration of visits (222.2 minutes) when compared to hospitals with small bed size (172.4 minutes) or those with medium bed size (166.5 minutes). The risk-adjusted results show that mean duration of visits on Mondays are longer by about 4 and 9 percents when compared to mean duration of visits on non-Monday workdays and weekends, respectively. Conclusions The duration of T&R ED visits varied significantly by admission hour, day of the week, patient volume, patient characteristics, hospital characteristics and area characteristics

An alternative to research, develop and deliver : rural development in Missouri as a case in point

Includes bibliographical references (pages 66-73)

Magnetic Properties of Linear Chain Systems: Metamagnetism of Single Crystal Co(pyridine)₂CI₂

The metamagnetic behavior of the low temperature properties of single crystal Co(pyridine)2Cl2 is discussed. At 1.25 K oriented single crystals exhibit a two-step metamagnetic transition at applied fields ~0.8 and 1.6 kG along the b-axis, a single transition at ~0.7 kG for applied fields along the a* axis, and a single transition at ~4.2 kG for an applied field along the c axis. Just above the transition fields a moment of 2µB/Co atom is measured for B0 parallel to the a* axis or b axis, and 0.4µB/Co atom is measured for the B0 parallel to the c axis. A large field dependent moment is observed at high fields. Many features of this compound closely mirror the behavior of CoCl2-2H20. However, the Co(pyridine)2Cl 2 has a much smaller interchain exchange, so that many features can be examined at lower fields. The basic features are consistent with a six-sublattice model for the ordered antiferromagnetic system. Measurements of magnetic moment versus temperature show that Co(pyridine)2Cl 2 does not obey a Curie-Weiss law even at relatively high temperatures

Proteomics and phylogenetic analysis of the cathepsin L protease family of the helminth pathogen Fasciola hepatica: Expansion of a repertoire of virulence-associated factors

Cathepsin L proteases secreted by the helminth pathogen Fasciola hepatica have functions in parasite virulence including tissue invasion and suppression of host immune responses. Using proteomics methods alongside phylogenetic studies we characterized the profile of cathepsin L proteases secreted by adult F. hepatica and hence identified those involved in host-pathogen interaction. Phylogenetic analyses showed that the Fasciola cathepsin L gene family expanded by a series of gene duplications followed by divergence that gave rise to three clades associated with mature adult worms (Clades 1, 2, and 5) and two clades specific to infective juvenile stages (Clades 3 and 4). Consistent with these observations our proteomics studies identified representatives from Clades 1, 2, and 5 but not from Clades 3 and 4 in adult F. hepatica secretory products. Clades 1 and 2 account for 67.39 and 27.63% of total secreted cathepsin Ls, respectively, suggesting that their expansion was positively driven and that these proteases are most critical for parasite survival and adaptation. Sequence comparison studies revealed that the expansion of cathepsin Ls by gene duplication was followed by residue changes in the S2 pocket of the active site. Our biochemical studies showed that these changes result in alterations in substrate binding and suggested that the divergence of the cathepsin L family produced a repertoire of enzymes with overlapping and complementary substrate specificities that could cleave host macromolecules more efficiently. Although the cathepsin Ls are produced as zymogens containing a prosegment and mature domain, all secreted enzymes identified by MS were processed to mature active enzymes. The prosegment region was highly conserved between the clades except at the boundary of prosegment and mature enzyme. Despite the lack of conservation at this section, sites for exogenous cleavage by asparaginyl endopeptidases and a Leu-Ser ↓ His motif for autocatalytic cleavage by cathepsin Ls were preserved. © 2008 by The American Society for Biochemistry and Molecular Biology, Inc

Positive In Vivo Selection of the HIV-1 Envelope Protein gp120 Occurs at Surface-Exposed Regions

The rapid evolution of human immunodeficiency virus (HIV) envelope represents a major challenge to vaccine and drug development, particularly because the underlying mechanisms are not completely understood. To explore whether distinct patterns of positive selection within the envelope protein glycoprotein (gp) 120 exist and are associated with functionally relevant domains, we conducted a long-term survey of sequence evolution in 20 HIV-1-infected persons who interrupted antiretroviral therapy. In total, 1753 clonal sequences encompassing the C2-V3-C3 region of gp120 were derived. Strikingly, positively selected amino acids mapped almost exclusively (P=.0003) to externally accessible residues on the gp120 crystal structure. The current understanding of envelope structure and function associates the main determinants of viral entry and the targets for neutralizing antibodies with these exterior regions of gp120, strongly suggesting that the observed adaptive evolution of these sites occurs in response to respective selective force

Extremely Inefficient Star Formation in the Outer Disks of Nearby Galaxies

(Abridged) We combine data from The HI Nearby Galaxy Survey and the GALEX Nearby Galaxy Survey to study the relationship between atomic hydrogen (HI) and far-ultraviolet (FUV) emission outside the optical radius (r25) in 17 spiral and 5 dwarf galaxies. In this regime, HI is likely to represent most of the ISM and FUV emission to trace recent star formation with little bias due to extinction, so that the two quantities closely trace the underlying relationship between gas and star formation rate (SFR). The azimuthally averaged HI and FUV intensities both decline with increasing radius in this regime, with the scale length of the FUV profile typically half that of the HI profile. Despite the mismatch in profiles, there is a significant spatial correlation (at 15" resolution) between local FUV and HI intensities; near r25 this correlation is quite strong, in fact stronger than anywhere inside r25, and shows a decline towards larger radii. The star formation efficiency (SFE) - defined as the ratio of FUV/HI and thus the inverse of the gas depletion time - decreases with galactocentric radius across the outer disks, though much shallower than across the optical disks. On average, we find the gas depletion times to be well above a Hubble time (~10^11 yr). We observe a clear relationship between FUV/HI and HI column in the outer disks, with the SFE increasing with increasing HI column. Despite observing systematic variations in FUV/HI, we find no clear evidence for step-function type star formation thresholds. When compared with results from inside r25, we find outer disk star formation to be distinct in several ways: it is extremely inefficient (depletion times of many Hubble times) with column densities and SFRs lower than found anywhere inside the optical disks. It appears that the HI column is one of, perhaps even the key environmental factor in setting the SFR in outer galaxy disks.Comment: Accepted for Publication in The Astronomical Journa

Full-field Chromatic Pupillometry for the Assessment of the Postillumination Pupil Response Driven by Melanopsin-Containing Retinal Ganglion Cells

Citation: Lei S, Goltz HC, Chandrakumar M, Wong AMF. Full-field chromatic pupillometry for the assessment of the postillumination pupil response driven by melanopsin-containing retinal ganglion cells. Invest Ophthalmol Vis Sci. 2014;55:4496-4503