Edema Induced by sPLA2 from <em>Crotalus durissus terrificus</em> Involves PLC and PKC Signaling, Activation of cPLA2, and Oxidative Stress

sPLA2 from Crotalus durissus terrificus venom, free of crotapotin (Cdt sPLA2), purified and isolated sPLA2, was able to significantly increase lipid peroxidation, which occurred simultaneously with increased arachidonic acid (AA) metabolism. In addition, MDA and AA levels were elevated at 15 min after Cdt sPLA2 injection and after peak edema (negative control). Thus, oxidative stress and ROS play important roles in the inflammation induced by Cdt sPLA2. On the other hand, edema induced by sPLA2 involves the direct and indirect mobilization of arachidonic acid by the involvement of phosphokinase C (PKC) and phospholipase C (PLC), which indirectly stimulates cytosolic PLA2 (cPLA2). We also observed that the specific antivenin against Cdt venom had no significant effect on the neutralization of induced edema compared to the natural products 5-caffeine-linoleic acid (5CQA) and dexamethasone (AACOCF3). Our results also indicate that there was improvement in the inhibition of edema of natural polyphenolic compounds compared to antivenin or inhibition of the enzymatic activity of sPLA2 due to the fact that 5CQA is a potent antioxidant compound. Thus, our results show a clear correlation between increased arachidonic acid metabolism and oxidative stress

Short communication: Genetic analysis of lactation curves in buffaloes, using Wood’s model

Aim of study: To estimate the heritability and genetic correlations for lactation curve traits in buffaloes.Area of study: The buffalo cows were raised on properties located in the states of São Paulo, Ceará and Rio Grande do Norte, Brazil.Material and methods: The individual parameters of Wood’s model ( , , and ) were obtained using a non-linear mixed model. Peak yield (PY), peak time (PT) and lactation persistency (LP) were also calculated. These individual parameters were employed in multi-trait analysis with the milk yield (MY) using Bayesian inference.Main results: The heritability estimates were of low to moderate magnitudes, with values ranging from 0.156 ( ) to 0.299 (PY). The estimates for genetic correlation between the Wood’s parameters and MY were of low to high magnitude and ranged from -0.533 (  and MY) to 0.983 (PY and MY).Research highlights: The heritability estimates obtained indicate that the traits studied can be used in animal breeding programs

Transcriptomic landscape of skin lesions in cutaneous leishmaniasis reveals a strong CD8(+) T cell immunosenescence signature linked to immunopathology

The severity of lesions that develop in patients infected by Leishmania braziliensis is mainly associated with a highly cytotoxic and inflammatory cutaneous environment. Recently, we demonstrated that senescent T and NK cells play a role in the establishment and maintenance of this tissue inflammation. Here, we extended those findings using transcriptomic analyses that demonstrate a strong co-induction of senescence and pro-inflammatory gene signatures in cutaneous leishmaniasis (CL) lesions. The senescence-associated signature was characterized by marked expression of key genes such as ATM, Sestrin 2, p16, p21 and p38. The cell type identification from deconvolution of bulk sequencing data showed that the senescence signature was linked with CD8+ effector memory and TEMRA subsets and also senescent NK cells. A key observation was that the senescence markers in the skin lesions are age-independent of patients and were correlated with lesion size. Moreover, a striking expression of the senescence-associated secretory phenotype (SASP), pro-inflammatory cytokine and chemokines genes was found within lesions that were most strongly associated with the senescent CD8 TEMRA subset. Collectively, our results confirm that there is a senescence transcriptomic signature in CL lesions and supports the hypothesis that lesional senescent cells have a major role in mediating immunopathology of the disease

Growth hormone 1 gene (GH1) polymorphisms as possible markers of the production potential of beef cattle using the Brazilian Canchim breed as a model

The growth hormone 1 gene (GH1) is a candidate gene for body weight and weight gain in cattle since it plays a fundamental role in growth regulation. We investigated the GH1 gene AluI and DdeI restriction enzyme polymorphisms, located 149 bp apart in the cattle genome, as possible markers of the production potential of Canchim crossbreed cattle, a 5/8 Charolais (Bos taurus) and 3/8 Nelore (Bos indicus) breed developed in Brazil, by evaluating the birth weight, weaning weight, yearling weight and plasma insulin-like growth factor-1 (IGF-1) concentration of 7 month to 10 months old Canchim calves (n = 204) of known genealogy and which had been genotyped for the AluI and DdeI markers. Our results showed significant effect (p < 0.05) between the homozygous DdeI+/DdeI+ polymorphism and the estimated breeding value for weaning weight (ESB-WW), while the AluI leucine homozygous (L/L) and leucine/valine (L/V) heterozygous polymorphisms showed no significant effect on the traits studied. The restriction sites of the two enzymes led to the formation of haplotypes which also exerted a significant effect (p < 0.05) on the ESB-WW, with the largest difference being 8.5 kg in favor of the homozygous L plus DdeI+/L plus DdeI+ genotype over the heterozygous L plus DdeI-/V plus DdeI+ genotype

Bibliometric analysis of themes competitive intelligence, knowledge management and organizational knowledge in Institutional Repository University of Brasília

Trata da análise dos temas Inteligência Competitiva (IC), Gestão do Conhecimento (GC) e Conhecimento Organizacional (CO), no Repositório Institucional da Universidade da Brasília (RIUnB), que está disponível em acesso aberto desde setembro de 2008. O objetivo é verificar a produção científica sobre as áreas de pesquisa, a partir dos documentos que constam no RIUnB. O método de trabalho consistiu nos estudos bibliométricos, a fim de se inteirar sobre a produção de trabalhos referentes ao conhecimento resultante do desenvolvimento de pesquisas no âmbito institucional, para conhecer a comunidade. Os resultados apontam para um crescimento notório desses temas na ciência da informação, com perspectiva de crescimento em outras áreas: administração, engenharia elétrica, educação e gestão social e trabalho, demonstrando sua potencial interdisciplinaridade; houve maior concentração de publicação nos temas gestão do conhecimento, conhecimento organizacional e inteligência competitiva, respectivamente, e existe uma concentração dessas publicações em três pesquisadores na comunidade da Faculdade de Ciência da Informação (FCI). Conclui-se que os temas são mais frequentes na área de ciência da informação, entretanto, nota-se o seu aparecimento em outras áreas do conhecimento, na UnB: administração, engenharia elétrica, educação e gestão social e do trabalho. Esses dados podem revelar uma tendência de consolidação dos temas de pesquisa em outras áreas. ________________________________________________________________________________________ ABSTRACTThis analysis of topics Competitive Intelligence (CI), Knowledge Management (KM) and Organizational Knowledge (CO) in the Institutional Repository of the University of Brasília (RIUnB) which is available in open access since September 2008. The aim is to check the scientific literature on the research areas from the documents listed in RIUnB. The working method consisted in bibliometric studies, in order to learn about the production of works relating to knowledge resulting from research development at the institutional level to know the community. The results indicate a remarkable growth of these themes in information science, with growth prospects in other areas: administration, electrical engineering, management and education and social work, demonstrating its potential interdisciplinarity, there was a higher concentration of publishing on issues of knowledge management, organizational knowledge and competitive intelligence, respectively, and there is a concentration of such publications in three community researchers in the Faculty of Information Science (FCI). We conclude that the issues are more frequent in the area of information science, however, note its appearance in other areas of knowledge in UNB: administration, electrical engineering, education and social management and labor. These data may reveal a trend of consolidation of research topics in other areas

ROTAS TECNOLÓGICAS EMPREGADAS NO APROVEITAMENTO DE RESÍDUOS DA INDÚSTRIA DA SOJA

O presente trabalho tem como objetivo realizar uma revisão bibliográfica acerca do potencial de aproveitamento de resíduos proveniente da agroindústria da soja por meio de diversosprocessos tecnológicos. O estudo apresenta as características fitofisionômicas, agrícolas e químicas da soja bem como uma análise minuciosa de suas atividades de produção, industrialização e consumo, de acordo com regiões e unidades federativas brasileiras. São apresentadas durante a revisão as operações unitárias do processo produtivo da agroindústria da soja. Esse trabalho também apresenta o potencial de aproveitamento da casca da soja por meio de rotas tecnológicas, buscando assim a redução da disposição dos resíduos sólidos industriais e agregando valor a essa importante cadeia produtiva

Comparte la felicidad, educando sobre sexualidad con ciudadanos y ciudadanas habitantes de calle

Curso de Especial InterésLos habitantes de calle (en adelante CHC) se han convertido en una problemática social debido a la desarticulación, violencia y pobreza de la sociedad colombiana. A partir de esta situación se diseñó y elaboró la cartilla “Comparte la felicidad, educando sobre sexualidad con Ciudadanos y Ciudadanas Habitantes de calle” que aborda los cuatro holones de la sexualidad: Vinculación afectiva, erotismo, género y reproductividad, con el objetivo de promover la salud sexual y reproductiva, y la prevención de Infecciones de transmisión sexual, incluido el VIH/SIDA. Para identificar el contenido de la cartilla se realizó una entrevista estructurada de la cual se obtuvo la información a incluir en la cartilla, posteriormente validada en la unidad OASIS.Curso de Especial Interés1. Resumen 2. Justificación 3. Marco teórico 4. Objetivos de la investigación 5. Métodología 6. Estudio de mercado 7. Resultados 8. Discusión 9. Conclusiones 10. Recomendaciones 11. Referencias 12. ApéndicesPregradoPsicólog

A new method to evaluate glenoid erosion in instable shoulder

Background: We aimed to establish values and parameters using multislice reconstruction in axial computerized tomography (CT) in order to quantify the erosion of the glenoid cavity in cases of shoulder instability. Methods: We studied two groups using CT. Group I had normal subjects and Group II had patients with shoulder instability. We measured values of the vertical segment, the superior horizontal, medial and inferior segments, and also calculated the ratio of the horizontal superior and inferior segments of the glenoid cavity in both normal subjects and those with shoulder instability. These variables were recorded during arthroscopy for cases with shoulder instability.\ud \ud \ud \ud Results\ud The mean values were 40.87 mm, 17.86 mm, 26.50 mm, 22.86 mm and 0.79 for vertical segment, the superior horizontal, medial and inferior segments, and the ratio between horizontal superior and inferior segments of the glenoid cavity respectively, in normal subjects. For subjects with unstable shoulders the mean values were 37.33 mm, 20.83 mm, 23.07 mm and 0.91 respectively. Arthroscopic measurements yielded an inferior segment value of 24.48 mm with a loss of 2.39 mm (17.57%). The ratio between the superior and inferior segments of the glenoid cavity was 0.79. This value can be used as a normative value for evaluating degree of erosion of the anterior border of the glenoid cavity. However, values found using CT should not be used on a comparative basis with values found during arthroscopy. Conclusions: Computerized tomographic measurements of the glenoid cavity yielded reliable values consistent with those in the literature.The funding body provided financial support to make all procedures and in the decision to submit the manuscript for publication