Yield-determining factors in high-solids enzymatic hydrolysis of lignocellulose

<p>Abstract</p> <p>Background</p> <p>Working at high solids (substrate) concentrations is advantageous in enzymatic conversion of lignocellulosic biomass as it increases product concentrations and plant productivity while lowering energy and water input. However, for a number of lignocellulosic substrates it has been shown that at increasing substrate concentration, the corresponding yield decreases in a fashion which can not be explained by current models and knowledge of enzyme-substrate interactions. This decrease in yield is undesirable as it offsets the advantages of working at high solids levels. The cause of the 'solids effect' has so far remained unknown.</p> <p>Results</p> <p>The decreasing conversion at increasing solids concentrations was found to be a generic or intrinsic effect, describing a linear correlation from 5 to 30% initial total solids content (w/w). Insufficient mixing has previously been shown not to be involved in the effect. Hydrolysis experiments with filter paper showed that neither lignin content nor hemicellulose-derived inhibitors appear to be responsible for the decrease in yields. Product inhibition by glucose and in particular cellobiose (and ethanol in simultaneous saccharification and fermentation) at the increased concentrations at high solids loading plays a role but could not completely account for the decreasing conversion. Adsorption of cellulases was found to decrease at increasing solids concentrations. There was a strong correlation between the decreasing adsorption and conversion, indicating that the inhibition of cellulase adsorption to cellulose is causing the decrease in yield.</p> <p>Conclusion</p> <p>Inhibition of enzyme adsorption by hydrolysis products appear to be the main cause of the decreasing yields at increasing substrate concentrations in the enzymatic decomposition of cellulosic biomass. In order to facilitate high conversions at high solids concentrations, understanding of the mechanisms involved in high-solids product inhibition and adsorption inhibition must be improved.</p

Development and evaluation of mould for double curved concrete elements

The present paper describes a concept for a reconfigurable mould surface which is designed to fit the needs of contemporary architecture. The core of the concept presented is a dynamic surface manipulated into a given shape using a digital signal created directly from the CAD drawing of the design. This happen fast, automatic and without production of waste, and the manipulated surface is fair and robust, eliminating the need for additional, manual treatment. Limitations to the possibilities of the flexible form are limited curvature and limited level of detail, making it especially suited for larger, double curved surfaces like facades or walls, where the curvature of each element is relatively small in comparison to the overall shape

Cell-wall structural changes in wheat straw pretreated for bioethanol production

Abstract Background Pretreatment is an essential step in the enzymatic hydrolysis of biomass and subsequent production of bioethanol. Recent results indicate that only a mild pretreatment is necessary in an industrial, economically feasible system. The Integrated Biomass Utilisation System hydrothermal pretreatment process has previously been shown to be effective in preparing wheat straw for these processes without the application of additional chemicals. In the current work, the effect of the pretreatment on the straw cell-wall matrix and its components are characterised microscopically (atomic force microscopy and scanning electron microscopy) and spectroscopically (attenuated total reflectance Fourier transform infrared spectroscopy) in order to understand this increase in digestibility. Results The hydrothermal pretreatment does not degrade the fibrillar structure of cellulose but causes profound lignin re-localisation. Results from the current work indicate that wax has been removed and hemicellulose has been partially removed. Similar changes were found in wheat straw pretreated by steam explosion. Conclusion Results indicate that hydrothermal pretreatment increases the digestibility by increasing the accessibility of the cellulose through a re-localisation of lignin and a partial removal of hemicellulose, rather than by disruption of the cell wall.</p

Valg af Basishavn i Danmark - En undersøgelse af 6 havne for RA (Royal Arctic)

Analyse til brug for Raoyl Artic's valg af basishavn i DanmarkAnalysen omfattede fire delundersøgelser: vurdering af nuveærende basishavn (Aalborg) benchmark af Aalborg Havn og 5 andre havne en undersøgelse af 1o kunders præferencer for de 6 havne Økonnomiske konsekvensvurderingerfor Raoyal Arctic, kunderne, Grønland Hjemmestyre og GrønlandResultaterne er konfidentiell

System for Real-Time Positioning and Monitoring of Fish in Commercial Marine Farms Based on Acoustic Telemetry and Internet of Fish (IoF)

Website: www.isope.orgpublishedVersio

An acoustic sensor transmitter for in situ assessment of water quality and fish behaviour during chemical treatment of a parasite-infected river system : tag design and practical use

publishedVersio