Development of the Renewal on the Standing Rock Sioux Reservation Project

On the Ground•The Standing Rock Sioux Reservation comprises 2.3 million acres, primarily rangeland, straddling the North Dakota–South Dakota border.•Natural resource management is economically and culturally important to the Standing Rock community.•Respecting traditional ways of thinking and placing stakeholders and their needs at the center are key aspects of project development.•Native Americans were the original natural resource managers on our rangelands, and their thoughts and expertise can provide guidance to rangeland managers now and in the future

Exploring Agricultural Production Systems and Their Fundamental Components with System Dynamics Modelling

Agricultural production in the United States is undergoing marked changes due to rapid shifts in consumer demands, input costs, and concerns for food safety and environmental impact. Agricultural production systems are comprised of multidimensional components and drivers that interact in complex ways to influence production sustainability. In a mixed-methods approach, we combine qualitative and quantitative data to develop and simulate a system dynamics model that explores the systemic interaction of these drivers on the economic, environmental and social sustainability of agricultural production. We then use this model to evaluate the role of each driver in determining the differences in sustainability between three distinct production systems: crops only, livestock only, and an integrated crops and livestock system. The result from these modelling efforts found that the greatest potential for sustainability existed with the crops only production system. While this study presents a stand-alone contribution to sector knowledge and practice, it encourages future research in this sector that employs similar systems-based methods to enable more sustainable practices and policies within agricultural production

Transposable elements modulate human RNA abundance and splicing via specific RNA-protein interactions

Background: Transposable elements (TEs) have significantly influenced the evolution of transcriptional regulatory networks in the human genome. Post-transcriptional regulation of human genes by TE-derived sequences has been observed in specific contexts, but has yet to be systematically and comprehensively investigated. Here, we study a collection of 75 CLIP-Seq experiments mapping the RNA binding sites for a diverse set of 51 human proteins to explore the role of TEs in post-transcriptional regulation of human mRNAs and lncRNAs via RNA-protein interactions. Results: We detect widespread interactions between RNA binding proteins (RBPs) and many families of TE-derived sequence in the CLIP-Seq data. Further, alignment coverage peaks on specific positions of the TE consensus sequences, illuminating a diversity of TE-specific RBP binding motifs. Evidence of binding and conservation of these motifs in the nonrepetitive transcriptome suggests that TEs have generally appropriated existing sequence preferences of the RBPs. Depletion assays for numerous RBPs show that TE-derived binding sites affect transcript abundance and splicing similarly to nonrepetitive sites. However, in a few cases the effect of RBP binding depends on the specific TE family bound; for example, the ubiquitously expressed RBP HuR confers transcript stability unless bound to an Alu element. Conclusions: Our meta-analysis suggests a widespread role for TEs in shaping RNA-protein regulatory networks in the human genome. Electronic supplementary material The online version of this article (doi:10.1186/s13059-014-0537-5) contains supplementary material, which is available to authorized users

Use of Ecological Sites in Managing Wildlife and Livestock: An Example with Prairie Dogs

On the Ground•The perception of prairie dogs among Native Americans living on the Standing Rock Sioux Reservation is mixed. Some Native Americans focus on the loss of forage productivity, whereas others are interested in the cultural and ecological aspects of prairie dogs.•The use of ecological sites may provide a mechanism for developing a management framework that would consider both livestock and prairie dogs.•The three ecological sites we surveyed had large differences in off-colony standing crop, but in 2 of the 3 years we surveyed, there were no differences between standing crop on-colony.•This suggests that management of prairie dogs on rangelands should focus on limiting prairie dogs on more productive ecological sites with less productive sites receiving less emphasis

Use of Ecological Sites in Managing Wildlife and Livestock: An Example with Prairie Dogs

On the Ground The perception of prairie dogs among Native Americans living on the Standing Rock Sioux Reservation is mixed. Some Native Americans focus on the loss of forage productivity, whereas others are interested in the cultural and ecological aspects of prairie dogs. The use of ecological sites may provide a mechanism for developing a management framework that would consider both livestock and prairie dogs. The three ecological sites we surveyed had large differences in off-colony standing crop, but in 2 of the 3 years we surveyed, there were no differences between standing crop on-colony. This suggests that management of prairie dogs on rangelands should focus on limiting prairie dogs on more productive ecological sites with less productive sites receiving less emphasis

Late-seeded cover crops in a semiarid environment: overyielding, dominance and subsequent crop yield

Interest in cover crops is increasing but information is limited on integrating them into crop rotations especially in the relatively short growing season on the northern Great Plains. A 3-yr research project, initiated in 2009 near Mandan, North Dakota, USA, evaluated (1) what impact cover crops may have on subsequent cash crops yields and (2) whether cover crop mixtures are more productive and provide additional benefits compared to cover crop monocultures. The study evaluated 18 different cover crop monocultures and mixtures that were seeded in August following dry pea (Pisum sativum L.). The following year, spring wheat (Triticum aestivum L.), corn (Zea mays L.), soybean (Glycine max L.) and field pea were seeded into the different cover crop treatments and a non-treated control. A lack of timely precipitation in 2009 resulted in a low cover crop yield of 17 g m2 compared to 100 and 77 g m2 in 2008 and 2010, respectively. Subsequent cash crop yield was not affected by late-seeded cover crops. Cool-season cover crop monocultures were more productive than warm-season mono-cultures and some mixtures in 2008 and 2010. Relative yield total did not differ from one in any cover crop mixture suggesting that overyielding did not occur. Species selection rather than species diversity was the most important contributor to cover crop yield. Cover crops can be grown following short-season cash crops in the northern Great Plains, but precipitation timing and species selection are critical

Co-reminiscing with a caregiver about a devastating tornado: Association with adolescent anxiety symptoms

This study explores the association between caregivers’ style of co-reminiscing with their adolescents about an EF4 tornado and youth anxiety symptoms several years following the disaster. Caregiver reward of their children’s emotional expression, defined as attending to and validating emotionally salient content, is generally associated with adaptive youth psychosocial outcomes. However, caregiver reward of youth recollections that are centered around the youth’s negative emotional expression could be an indicator that both caregivers and adolescents are engaged in co-rumination regarding negative emotional experiences. This process may contribute to relatively higher levels of anxiety over time. Adolescents (N = 169) drawn from an ongoing study for aggressive youth (ages 12 to 17; 82% African American) provided individual recollections about their experiences during a devastating tornado 4 to 5 years following the disaster. Caregivers and youth then co-reminisced about their tornado-related experiences. Individual youth recollections were coded for negative personal impact and use of negative emotion words; caregiver–adolescent conversations were coded for caregiver reward of negative emotional expression. Youth who noted more negative personal impacts and used more negative emotion words were higher in parent-rated youth anxiety, and these associations were moderated by caregiver reward of negative emotional expression. The associations between youth recollection qualities and anxiety emerged only when caregivers exhibited high levels of reward of negative emotional expression. These patterns were generally stronger for girls compared to boys. Findings suggest that excessively discussing and rehashing negative experiences, especially several years after the disaster, may be a risk factor for anxiety among disaster-exposed adolescents

Biomass round bales infield aggregation logistics scenarios

Biomass bales often need to be aggregated (collected into groups and transported) to a field-edge stack or a temporary storage before utilization. Several logistics scenarios for aggregation involving equipment and aggregation strategies were modeled and evaluated. Cumulative Euclidean distance criteria evaluated the various aggregation scenarios. Application of a single-bale loader that aggregated bales individually was considered as the “control” scenario with which others were compared. A computer simulation program developed determined bale coordinates in ideal and random layouts that evaluated aggregation scenarios. Simulation results exhibited a “diamond pattern” of bales on ideal layout and a “random pattern” emerged when ≥ 10% variation was introduced. Statistical analysis revealed that the effect of field shape, swath width, biomass yield, and randomness on bale layout did not affect aggregation logistics, while area and number of bales handled had significant effects. Number of bales handled in the direct method significantly influenced the efficiency. Self-loading bale picker with minimum distance path (MDP, 80%) and parallel transport of loader and truck with MDP (78%) were ranked the highest, and single-bale central grouping the lowest (29%) among 19 methods studied. The MDP was found significantly more efficient (4%-16%) than the baler path. Simplistic methods, namely a direct triple-bale loader with MDP (64%-66%), or a loader and truck handling six bales running parallel with MDP (75%-82%) were highly efficient. Great savings on cumulative distances that directly influence time, fuel, and cost were realized when the number of bales handled was increased or additional equipment was utilized

Synthesis Paper: Targeted Livestock Grazing: Prescription for Healthy Rangelands

Targeted livestock grazing is a proven tool for manipulating range land vegetation, and current knowledge about targeted livestock grazing is extensive and expanding rapidly. Targeted grazing prescriptions optimize the timing, frequency, intensity, and selectivity of grazing (or browsing) in combinations that purposely exert grazing/ browsing pressure on specific plant species or portions of the landscape. Targeted grazing differs from traditional grazing management in that the goal of targeted grazing is to apply defoliation or trampling to achieve specific vegetation management objectives,whereas the goal of traditional livestock grazing management is generally the production of livestock commodities. A shared aim of targeted livestock grazing and traditional grazing management is to sustain healthy soils, flora, fauna, and water resources that, in turn, can sustain natural ecological processes (e.g., nutrient cycle, water cycle, energy flow). Targeted grazing prescriptions integrate knowledge of plant ecology, livestock nutrition, and livestock foraging behavior. Livestock can be focused on target areas through fencing, herding, or supplement placement. Although practices can be developed to minimize the impact of toxins contained in target plants, the welfare of the animals used in targeted grazing must be a priority. Monitoring is needed to determine if targeted grazing is successful and to refine techniques to improve efficacy and efficiency. Examples of previous research studies and approaches are presented to highlight the ecological benefits that can be achieved when targeted grazing is applied properly. These cases include ways to suppress invasive plants and ways to enhance wildlife habitat and biodiversity. Future research should address the potential to select more adapted and effective livestock for targeted grazing and the associated animal welfare concerns with this practice. Targeted livestock grazing provides land managers a viable alternative to mechanical, chemical, and prescribed fire treatments to manipulate range land vegetation