Multiparametric in vitro genotoxicity assessment of different variants of amorphous silica nanomaterials in rat alveolar epithelial cells

The hazard posed to human health by inhaled amorphous silica nanomaterials (aSiO2 NM) remains uncertain. Herein, we assessed the cyto- and genotoxicity of aSiO2 NM variants covering different sizes (7, 15, and 40 nm) and surface modifications (unmodified, phosphonate-, amino- and trimethylsilyl-modified) on rat alveolar epithelial (RLE-6TN) cells. Cytotoxicity was evaluated at 24 h after exposure to the aSiO2 NM variants by the lactate dehydrogenase (LDH) release and WST-1 reduction assays, while genotoxicity was assessed using different endpoints: DNA damage (single- and double-strand breaks [SSB and DSB]) by the comet assay for all aSiO2 NM variants; cell cycle progression and γ-H2AX levels (DSB) by flow cytometry for those variants that presented higher cytotoxic and DNA damaging potential. The variants with higher surface area demonstrated a higher cytotoxic potential (SiO2_7, SiO2_15_Unmod, SiO2_15_Amino, and SiO2_15_Phospho). SiO2_40 was the only variant that induced significant DNA damage on RLE-6TN cells. On the other hand, all tested variants (SiO2_7, SiO2_15_Unmod, SiO2_15_Amino, and SiO2_40) significantly increased total γ-H2AX levels. At high concentrations (28 µg/cm2), a decrease in G0/G1 subpopulation was accompanied by a significant increase in S and G2/M sub-populations after exposure to all tested materials except for SiO2_40 which did not affect cell cycle progression. Based on the obtained data, the tested variants can be ranked for its genotoxic DNA damage potential as follows: SiO2_7 = SiO2_40 = SiO2_15_Unmod > SiO2_15_Amino. Our study supports the usefulness of multiparametric approaches to improve the understanding on NM mechanisms of action and hazard prediction

Comparison of atmospheric CO, CO₂ and CH₄ measurements at the Schneefernerhaus and the mountain ridge at Zugspitze

The CO, CO2 and CH4 mole fractions have been measured since 2002 at the Environmental Research Station Schneefernerhaus, which is located approximately 300 m below the summit of Zugspitze in the German Alps. Although the station is located remotely at an altitude of 2650 m a.s.l., local pollution events by snow blowers and snow groomers can be detected in the high temporal-resolution time series of seconds or minutes. Therefore, a time-consuming flagging process, carried out manually by the station manager, is necessary. To examine local influences and the effectiveness of data flagging, a 290 m long intake line to the higher Zugspitze ridge was used to measure CO, CO2 and CH4 mole fractions at a potentially less polluted location between October 2018 and October 2020. The comparison of these two time series shows that the mountain ridge measurement is almost unaffected by local pollution. It also demonstrates that the influence of local pollution events on the Schneefernerhaus measurements is successfully removed by the station manager. Only a small deviation of up to 0.24 ppm can be observed during the day between the CO2 time series of the Schneefernerhaus and the mountain ridge in winter, probably due to anthropogenic sources.</p

Nanoparticle release from dental composites

Dental composites typically contain high amounts (up to 60vol.%) of nanosized filler particles. There is a current concern that dental personnel (and patients) may inhale nanosized dust particles (10(6)cm(-3)). The median diameter of airborne composite dust varied between 38 and 70nm. Electron microscopic and energy dispersive X-ray analysis confirmed that the airborne particles originated from the composite, and revealed that the dust particles consisted of filler particles or resin or both. Though composite dust exhibited no significant oxidative reactivity, more toxicological research is needed. To conclude, on manipulation with the bur, dental composites release high concentrations of nanoparticles that may enter deeply into the lungs.publisher: Elsevier articletitle: Nanoparticle release from dental composites journaltitle: Acta Biomaterialia articlelink: http://dx.doi.org/10.1016/j.actbio.2013.09.044 content_type: article copyright: Copyright © 2013 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.status: publishe