32 research outputs found
Rezension: Phillipp Mayring, GĂĽnter L. Huber, Leo GĂĽrtler, Mechthild Kiegelmann (Hrsg.): Mixed Methodology in Psychological Research
Rezension: Vera Luif, Gisela Thoma, Brigitte Boothe (Hrsg.): Beschreiben - Erschließen - Erläutern. Psychotherapieforschung als qualitative Wissenschaft
From Life-Threatening Experiences to Ideas of Rescue: Coping with “Trajectories of Suffering†in Adult Acute Leukaemia Survivors
We investigated the illness records and life stories of 17 leukaemia survivors using narrative autobiographical interviews. Audio tapes were transcribed and analysed according to qualitative methodology. Using the sociological concept of “trajectory of suffering†(TOS) as a means of analysis we focused on the survivors’ mechanisms of psychosocial adaptation including integration of disease-related experiences as part of their autobiographical narration. Verbal data show how the diagnosis pulls affected people out of their everyday life from healthy, strong and with plans for the future to seriously ill, weak and facing death, and thus suspends their self-confidence and social action competence. Analysing the interview transcripts we found six categories of coping with TOS: (1) personal meaningful nourishments, (2) challenging experience with significant others, (3), courage to persevere, (4) family support, (5) dramatic family events, and (6) dreams. The results of our study demonstrate that the cancer is still a dark shadow over the lives of all survivors. They are discussed in the context of coping theory postulating creativity as an up to now underestimated resource of coping behaviour. As a consequence it seems to be vital that medical staff should recognize and discuss these individual needs and feelings of their patients in daily clinical practice
Investigation of Body Development in Growing Holstein Heifers With Special Emphasis on Body Fat Development Using Bioelectrical Impedance Analysis
This study analyzed skeletal development, body condition, and total body fat
development of growing heifers. A total of 144 female primiparous Holstein cattle from
four commercial dairy farms with different degrees of stillbirth rates were examined during
the rearing period. This included measurements in body condition, fat tissue, metabolic,
and endocrine factors. Pelvic measurements and the sacrum height were analyzed to
assess skeletal development. The body condition was classified via body condition
scoring, bioelectrical impedance analysis (BIA), back fat thickness measurements, and
the body mass. For the first time, BIA was used as an appropriate method to evaluate
the fat tissue content of cattle throughout the rearing period. This analysis technique can
be performed on heifers aged 8–15 months. Throughout that period, the fat content
decreased while the skeletal development increased. In addition, high free fatty acid
concentrations in serum of the animals with high frame development were found,
supporting our hypothesis that stored energy of body fat deposits is used for skeletal
growth. Furthermore, we were able to demonstrate complex endocrine relationships
between fat metabolism and skeletal growth by using specific markers, such as leptin,
insulin growth factor-1 (IGF-1), and estradiol (E2). Food analysis showed high crude
protein (CP) levels in the total mixed ration above recommendation for daily protein intake
of all farms. However, there was a positive correlation between CP and the body frame
measurements in our study. In summary, we established a novel regression formula for
BIA analysis (“BIA-Heine”) in heifers to evaluate the body composition throughout different
ages and physiological stages in the development of heifers. This special formula allows
the evaluation of fat tissue without a whole-body analysis and therefore provides an
innovative technique for animal welfare support
A collection of bacterial isolates from the pig intestine reveals functional and taxonomic diversity.
Our knowledge about the gut microbiota of pigs is still scarce, despite the importance of these animals for biomedical research and agriculture. Here, we present a collection of cultured bacteria from the pig gut, including 110 species across 40 families and nine phyla. We provide taxonomic descriptions for 22 novel species and 16 genera. Meta-analysis of 16S rRNA amplicon sequence data and metagenome-assembled genomes reveal prevalent and pig-specific species within Lactobacillus, Streptococcus, Clostridium, Desulfovibrio, Enterococcus, Fusobacterium, and several new genera described in this study. Potentially interesting functions discovered in these organisms include a fucosyltransferase encoded in the genome of the novel species Clostridium porci, and prevalent gene clusters for biosynthesis of sactipeptide-like peptides. Many strains deconjugate primary bile acids in in vitro assays, and a Clostridium scindens strain produces secondary bile acids via dehydroxylation. In addition, cells of the novel species Bullifex porci are coccoidal or spherical under the culture conditions tested, in contrast with the usual helical shape of other members of the family Spirochaetaceae. The strain collection, called 'Pig intestinal bacterial collection' (PiBAC), is publicly available at www.dsmz.de/pibac and opens new avenues for functional studies of the pig gut microbiota
Identifying Efficient Clostridium difficile Toxin A Binders with a Multivalent Neo-Glycoprotein Glycan Library
Electrochemical Impedance Spectroscopy Using Interdigitated Gold-Polypyrrole Electrode Combination
The catabolism of 3,3'-thiodipropionic acid in Variovorax paradoxus strain TBEA6: A proteomic analysis.
Variovorax paradoxus strain TBEA6 is one of the few organisms known to utilize 3,3'-thiodipropionate (TDP) as the only source of carbon and energy. It cleaves TDP to 3-mercaptopropionate (3MP), which is a direct precursor for polythioester synthesis. To establish this process in V. paradoxus TBEA6, it is crucial to unravel its TDP metabolism. Therefore, a proteomic approach with subsequent deletion of interesting genes in the bacterium was chosen. Cells were cultivated with D-gluconate, TDP or 3-sulfinopropionate as the only carbon sources. Proteins with high abundances in gels of cells cultivated with either of the organic sulfur compounds were analyzed further. Thereby, we did not only confirm parts of the already postulated TDP metabolism, but also eight new protein candidates for TDP degradation were detected. Deletions of the corresponding genes (two enoyl-CoA hydratases (Ech-20 and Ech-30), an FK506-binding protein, a putative acetolactate synthase, a carnitinyl-CoA dehydratase, and a putative crotonase family protein) were obtained. Only the deletions of both Ech-20 and Ech-30 led to a TDP negative phenotype. The deletion mutant of VPARA_05510, which encodes the putative crotonase family protein showed reduced growth with TDP. The three genes are located in one cluster with genes proven to be involved in TDP metabolism. Thermal shift assays showed an increased stability of Ech-20 with TDP-CoA but not with TDP. These results indicate that Ech-20 uses TDP-CoA as a substrate instead of TDP. Hence, we postulate a new putative pathway for TDP metabolism. Ech-30 interacts with neither TDP-CoA nor TDP but might interact with other CoA-activated intermediates of the proposed pathway. Further enzyme characterization is necessary to unravel the complete pathway from TDP to 3MP