Next Steps to Reform the Regulations Governing Offshore Oil and Gas Planning and Leasing

The Department of the Interior manages offshore oil and gas activities in federal waters. While the agency has proposed and/or enacted important improvements to the rules that govern some of those activities, it has not modernized the regulations that govern offshore oil and gas planning, lease sales, or the review and permitting of exploratory drilling. These phases of the process are overseen by the Bureau of Ocean Energy Management (BOEM), and, as was shown in our earlier publication on this topic, are ineffective and in need of modernization. In this Article, we argue that fundamental reform is necessary and highlight a series of key themes and topics that must be addressed to improve the regulatory process and promote better, more consistent management outcomes. While the Article draws on examples from frontier areas—in particular the U.S. Arctic Ocean—the recommended changes would apply to and benefit all areas of the OCS

Crystal structure of (S)-5,7-diphenyl-4,7-dihydrotetrazolo[1,5-a]pyrimidine

Acknowledgements The authors thank Christopher Daley, A. Rheingold and C. Moore (UCSD) for the data collection. Funding for this work was provided by the University of California, Merced and the National Science Foundation (CHE-1300686)Peer reviewedPublisher PD

Reality and Computation in Schubert Calculus

The Mukhin-Tarasov-Varchenko Theorem (previously the Shapiro Conjecture) asserts that a Schubert problem has all solutions distinct and real if the Schubert varieties involved osculate a rational normal curve at real points. When conjectured, it sparked interest in real osculating Schubert calculus, and computations played a large role in developing the surrounding theory. Our purpose is to uncover generalizations of the Mukhin-Tarasov-Varchenko Theorem, proving them when possible. We also improve the state of the art of computationally solving Schubert problems, allowing us to more effectively study ill-understood phenomena in Schubert calculus. We use supercomputers to methodically solve real osculating instances of Schubert problems. By studying over 300 million instances of over 700 Schubert problems, we amass data significant enough to reveal generalizations of the Mukhin-Tarasov- Varchenko Theorem and compelling enough to support our conjectures. Combining algebraic geometry and combinatorics, we prove some of these conjectures. To improve the efficiency of solving Schubert problems, we reformulate an instance of a Schubert problem as the solution set to a square system of equations in a higher- dimensional space. During our investigation, we found the number of real solutions to an instance of a symmetrically defined Schubert problem is congruent modulo four to the number of complex solutions. We proved this congruence, giving a generalization of the Mukhin-Tarasov-Varchenko Theorem and a new invariant in enumerative real algebraic geometry. We also discovered a family of Schubert problems whose number of real solutions to a real osculating instance has a lower bound depending only on the number of defining flags with real osculation points. We conclude that our method of computational investigation is effective for uncovering phenomena in enumerative real algebraic geometry. Furthermore, we point out that our square formulation for instances of Schubert problems may facilitate future experimentation by allowing one to solve instances using certifiable numerical methods in lieu of more computationally complex symbolic methods. Additionally, the methods we use for proving the congruence modulo four and for producing a

Reaction monitoring via benchtop nuclear magnetic resonance spectroscopy: A practical comparison of on-line stopped-flow and continuous-flow sampling methods

The ability for nuclear magnetic resonance (NMR) spectroscopy to provide quantitative, structurally rich information makes this spectroscopic technique an attractive reaction monitoring tool. The practicality of NMR for this type of analysis has only increased in the recent years with the influx of commercially available benchtop NMR instruments and compatible flow systems. In this study, we aim to compare 19F NMR reaction profiles acquired under both on-line continuous-flow and stopped-flow sampling methods, with modern benchtop NMR instrumentation, and two reaction systems: a homogeneous imination reaction and a biphasic activation of a carboxylic acid to acyl fluoride. Reaction trends with higher data density can be acquired with on-line continuous-flow analyses, and this work highlights that representative reaction trends can be acquired without any correction when monitoring resonances with a shorter spin–lattice relaxation time (T1), and with the used flow conditions. On-line stopped-flow analyses resulted in representative reaction trends in all cases, including the monitoring of resonances with a long T1, without the need of any correction factors. The benefit of easier data analysis, however, comes with the cost of time, as the fresh reaction solution must be flowed into the NMR system, halted, and time must be provided for spins to become polarized in the instrument's external magnetic field prior to spectral measurement. Results for one of the reactions were additionally compared with the use of a high-field NMR.publishedVersio

Extraterrestrial Amino Acids Identified in Metal-Rich CH and CB Carbonaceous Chondrites from Antarctica

Carbonaceous chondrites contain numerous indigenous organic compounds and could have been an important source of prebiotic compounds required for the origin of life on Earth or elsewhere. Extraterrestrial amino acids have been reported in five of the eight groups of carbonaceous chondrites and are most abundant in CI, CM, and CR chondritesbut are also present in the more thermally altered CV and CO chondrites. We report the abundance, distribution, and enantiomeric and isotopic compositions of simple primary amino acids in six metal-rich CH and CB carbonaceous chondrites that have not previously been investigated for amino acids: Allan Hills (ALH) 85085 (CH3), Pecora Escarpment(PCA) 91467 (CH3), Patuxent Range (PAT) 91546 (CH3), MacAlpine Hills (MAC) 02675(CBb), Miller Range (MIL) 05082 (CB), and Miller Range (MIL) 07411 (CB). Amino acid abundances and carbon isotopic values were obtained by using both liquid chromatography time-of-flight mass spectrometry and fluorescence, and gas chromatography isotope ratiomass spectrometry. The (delta D, delta C-13, delta N-15) ratios of multiple amino acids fall outside of the terrestrial range and support their extraterrestrial origin. Extracts of CH chondrites were found to be particularly rich in amino acids (1316 parts per million, ppm) while CB chondrite extracts had much lower abundances (0.22 ppm). The amino acid distributions of the CH and CB chondrites were distinct from the distributions observed in type 2 and 3 CM and CR chondrites and contained elevated levels of beta-, gamma-, and delta-amino acids compared to the corresponding alpha-amino acids, providing evidence that multiple amino acid formation mechanisms were important in CH and CB chondrites

Iterative in Situ Click Chemistry Assembles a Branched Capture Agent and Allosteric Inhibitor for Akt1

We describe the use of iterative in situ click chemistry to design an Akt-specific branched peptide triligand that is a drop-in replacement for monoclonal antibodies in multiple biochemical assays. Each peptide module in the branched structure makes unique contributions to affinity and/or specificity resulting in a 200 nM affinity ligand that efficiently immunoprecipitates Akt from cancer cell lysates and labels Akt in fixed cells. Our use of a small molecule to preinhibit Akt prior to screening resulted in low micromolar inhibitory potency and an allosteric mode of inhibition, which is evidenced through a series of competitive enzyme kinetic assays. To demonstrate the efficiency and selectivity of the protein-templated in situ click reaction, we developed a novel QPCR-based methodology that enabled a quantitative assessment of its yield. These results point to the potential for iterative in situ click chemistry to generate potent, synthetically accessible antibody replacements with novel inhibitory properties