Studies on the Life Cycle and Transmission of \u3ci\u3eCougourdella\u3c/i\u3e Sp., A Microsporidian Parasite of \u3ci\u3eGlossosoma Nigrior\u3c/i\u3e (Trichoptera: Glossosomatidae)

The trichopteran Glossosoma nigrior, the dominant benthic invertebrate grazer in Michigan trout streams, hosts a microsporidian (Protozoa) pathogen, Cougourdella sp., which strongly regulates the population density of larvae in the stream. In order to better understand the interactions between these two species, two possible modes of pathogen transmission, oral and transovum, were investigated. While both sexes of adult G. nigrior were found to be infected with mature environmental spores, spores were not found associated with reproductive tissue. This suggests that transovum transmission does not occur in this system. Glossosoma nigrior, when ex- posed to viable spores taken from infected larvae, did not produce Cougourdella sp. infections, which suggests that oral transmission also does not occur. It is possible that an intermediate host is required

Contemporary Evolutionary Divergence for a Protected Species following Assisted Colonization

Contemporary evolution following assisted colonization may increase the probability of persistence for refuge populations established as a bet-hedge for protected species. Such refuge populations are considered "genetic replicates" that might be used for future re-colonization in the event of a catastrophe in the native site. Although maladaptive evolutionary divergence of captive populations is well recognized, evolutionary divergence of wild refuge populations may also occur on contemporary time scales. Thus, refuge populations may lose their "value" as true genetic replicates of the native population. Here, we show contemporary evolutionary divergence in body shape in an approximately 30-year old refuge population of the protected White Sands pupfish (Cyprinodon tularosa) resulting in a body-shape mismatch with its native environment.Geometric morphometic data were collected from C. tularosa cultures raised in experimental mesocosms. Cultures were initiated with fish from the two native populations, plus hybrids, in high or low salinity treatments representing the salinities of the two native habitats. We found that body shape was heritable and that shape variation due to phenotypic plasticity was small compared to shape variation due to population source. C. tularosa from the high salinity population retained slender body shapes and fish from the low salinity population retained deep body shapes, irrespective of mesocosm salinity. These data suggest that the observed divergence of a recently established pupfish population was not explained by plasticity. An analysis of microsatellite variation indicated that no significant genetic drift occurred in the refuge population, further supporting the adaptive nature of changes in body shape. These lines of evidence suggest that body shape divergence of the refuge population reflects a case of contemporary evolution (over a 30-year period).These results suggest assisted colonization can introduce novel, and/or relaxed selection, and lead to unintended evolutionary divergence

Studies on the Life Cycle and Transmission of \u3ci\u3eCougourdella\u3c/i\u3e Sp., A Microsporidian Parasite of \u3ci\u3eGlossosoma Nigrior\u3c/i\u3e (Trichoptera: Glossosomatidae)

The trichopteran Glossosoma nigrior, the dominant benthic invertebrate grazer in Michigan trout streams, hosts a microsporidian (Protozoa) pathogen, Cougourdella sp., which strongly regulates the population density of larvae in the stream. In order to better understand the interactions between these two species, two possible modes of pathogen transmission, oral and transovum, were investigated. While both sexes of adult G. nigrior were found to be infected with mature environmental spores, spores were not found associated with reproductive tissue. This suggests that transovum transmission does not occur in this system. Glossosoma nigrior, when ex- posed to viable spores taken from infected larvae, did not produce Cougourdella sp. infections, which suggests that oral transmission also does not occur. It is possible that an intermediate host is required

Data from: Genetic divergence along the speciation continuum: the transition from host race to species in Rhagoletis (Diptera: Tephritdae)

Studies of related populations varying in their degrees of reproductive isolation can provide insights into speciation. Here, the transition from partially isolated host races to more fully separated sibling species is investigated by comparing patterns of genetic differentiation between recently evolved (∼150 generations) apple and ancestral hawthorn-infesting populations of Rhagoletis pomonella to their sister taxon, the undescribed flowering dogwood fly attacking Cornus florida. No fixed or diagnostic private alleles differentiating the three populations were found at any of 23 microsatellites and ten allozymes scored. Nevertheless, allele frequency differences were sufficient across loci for flowering dogwood fly populations from multiple localities to form a diagnosable genotypic cluster distinct from apple and hawthorn flies, indicative of species status. Genome-wide patterns of differentiation were correlated between the host races and species pair comparisons along the majority of chromosomes, suggesting that similar disruptive selection pressures affect most loci. However, differentiation was more pronounced, with some additional regions showing elevated divergence, for the species pair comparison. Our results imply that Rhagoletis sibling species such as the flowering dogwood fly represent host races writ-large, with the transition to species status primarily resulting from increased divergence of the same regions separating apple and hawthorn flies

Powell_etal_TableS1_forDRYAD

Microsatellite allele frequencies for the 23 loci analyzed in study for 17 field sites, representing seven hawthorn- and four apple-infesting populations of R. pomonella, and six flowering dogwood fly populations. Collection site and site# are explained in Table 1 and Figs. S1 and 3 of the associated manuscript.. Allele numbers represent the size (in base pairs) of the PCR amplified DNA fragment (including the repeat sequence) generated using locus specific primers for each microsatellite, as determined by capillary electrophoresis on a Beckman-Coulter CEQ8000. Bolded numbers designate alleles that were pooled together to form one of the two alternative allele classes analyzed at a locus. Summary statistics, heterozygosity H and allelic richness A, calculated using rarefaction standardized to samples of 20 individuals in ADZE 1.0 (Szpeich et al. 2008) are also presented