Charakterisierung der chromosomalen Bandenaufspaltung an den humanen Chromosomen 5, 6, 18, 19, 20 und X

Die Humanzytogenetik, die sich als eigenständige Forschungs-richtung innerhalb der Humangenetik etabliert hat, befasst sich mit der Darstellung und Analyse der menschlichen Chromosomen. Zytogenetische Aufarbeitungsmethoden, sowie eine Vielzahl von Bänderungsmethoden prägten das heutige Verständnis vom Verhalten der Chromosomen sowohl während der Präparation, als auch im Verlauf des Zellzyklus. Die Beschreibung der zellzyklus-gebundenen Aufspaltung chromosomaler Banden in ihre Subbanden ist in der human-zytogenetischen Diagnostik und Forschung von grundlegender Bedeutung. Die Reihenfolge dieser Aufspaltung – im Folgenden Hierarchie der Bandenaufspaltung genannt – ist für die menschlichen Chromosomen im International System for Human Cytogenetic Nomenclature (ISCN 1995) dokumentiert. Jedoch basiert diese Auflistung lediglich auf rein morphologischen Vergleichen der chromosomalen Bänderung auf 3 unterschiedlich hohen Auflösungsstufen (400, 550 und 850 Banden pro haploiden Chromosomensatz) und deren daraufhin weitgehend willkürlich festgelegten Nomenklatur, nicht jedoch auf systematischen Untersuchungen, welche zeigen würden, wie sich die einzelnen Banden in ihre Subbanden in Relation zur chromosomalen Länge aufspalten. Da bereits gezeigt werden konnte, dass diese allgemein akzeptierte und angewandte Nomenklatur zu einigen missverstandenen Schieflagen im Bezug auf unsere Vorstellung vom Verhalten der Chromosomen führte, wie etwa der Tatsache, dass entgegen der, in der ISCN veröffentlichten Angaben, aus giemsa-hellen Banden keine Subbanden hervorgehen können, bestehen berechtigte Zweifel an der biologischen Korrektheit dieser Daten. In der vorliegenden Arbeit wurde erstmals die Hierarchie der Aufspaltung von chromosomalen Banden an ausgewählten menschlichen lymphozytären Chromosomen, den Chromosomen 5, 6, 18, 19, 20 und X, systematisch untersucht. Durch die Kombination aus Multicolor-banding- und GTG-Bänderungs-Analysen auf den, der ISCN 1995 entsprechenden Bandenniveaus, konnte für die genannten Chromosomen erstmals die tatsächliche Herkunft der Subbanden nachvollziehbar wiedergegeben werden. Die Ergebnisse führten zu einem besseren Verständnis der Aufspaltung chromosomaler Banden und zu einer wesentlichen Korrektur, der in der ISCN 1995 angegebenen Nomenklatur

The hierarchically organized splitting of chromosomal bands for all human chromosomes

<p>Abstract</p> <p>Background</p> <p>Chromosome banding is widely used in cytogenetics. However, the biological nature of hierarchically organized splitting of chromosomal bands of human chromosomes is an enigma and has not been, as yet, studied.</p> <p>Results</p> <p>Here we present for the first time the hierarchically organized splitting of chromosomal bands in their sub-bands for all human chromosomes. To do this, array-proved multicolor banding (aMCB) probe-sets for all human chromosomes were applied to normal metaphase spreads of three different G-band levels. We confirmed for all chromosomes to be a general principle that only Giemsa-dark bands split into dark and light sub-bands, as we demonstrated previously by chromosome stretching. Thus, the biological band splitting is in > 50% of the sub-bands different than implemented by the ISCN nomenclature suggesting also a splitting of G-light bands. Locus-specific probes exemplary confirmed the results of MCB.</p> <p>Conclusion</p> <p>Overall, the present study enables a better understanding of chromosome architecture. The observed difference of biological and ISCN band-splitting may be an explanation why mapping data from human genome project do not always fit the cytogenetic mapping.</p

Early Embryonic Chromosome Instability Results in Stable Mosaic Pattern in Human Tissues

The discovery of copy number variations (CNV) in the human genome opened new perspectives on the study of the genetic causes of inherited disorders and the aetiology of common diseases. Here, a single-cell-level investigation of CNV in different human tissues led us to uncover the phenomenon of mitotically derived genomic mosaicism, which is stable in different cell types of one individual. The CNV mosaic ratios were different between the 10 individuals studied. However, they were stable in the T lymphocytes, immortalized B lymphoblastoid cells, and skin fibroblasts analyzed in each individual. Because these cell types have a common origin in the connective tissues, we suggest that mitotic changes in CNV regions may happen early during embryonic development and occur only once, after which the stable mosaic ratio is maintained throughout the differentiated tissues. This concept is further supported by a unique study of immortalized B lymphoblastoid cell lines obtained with 20 year difference from two subjects. We provide the first evidence of somatic mosaicism for CNV, with stable variation ratios in different cell types of one individual leading to the hypothesis of early embryonic chromosome instability resulting in stable mosaic pattern in human tissues. This concept has the potential to open new perspectives in personalized genetic diagnostics and can explain genetic phenomena like diminished penetrance in autosomal dominant diseases. We propose that further genomic studies should focus on the single-cell level, to better understand the aetiology of aging and diseases mediated by somatic mutations

The Human Genome Puzzle – the Role of Copy Number Variation in Somatic Mosaicism

The discovery of copy number variations (CNV) in the human genome opened new perspectives in the study of the genetic causes of inherited disorders and the etiology of common diseases. Differently patterned instances of somatic mosaicism in CNV regions have been shown to be present in monozygotic twins and throughout different tissues within an individual. A single-cell-level investigation of CNV in different human cell types led us to uncover mitotically derived genomic mosaicism, which is stable in different cell types of one individual. A unique study of immortalized B-lymphoblastoid cell lines obtained with 20 year interval from the same two subjects shows that mitotic changes in CNV regions may happen early during embryonic development and seem to occur only once, as levels of mosaicism remained stable. This finding has the potential to change our concept of dynamic human genome variation. We propose that further genomic studies should focus on the single-cell level, to understand better the etiology and physiology of aging and diseases mediated by somatic variations

Elucidating the genetic architecture of Adams-Oliver syndrome in a large European cohort

Adams-Oliver syndrome (AOS) is a rare developmental disorder, characterized by scalp aplasia cutis congenita (ACC) and transverse terminal limb defects (TTLD). Autosomal dominant forms of AOS are linked to mutations in ARHGAP31, DLL4, NOTCH1 or RBPJ, while DOCK6 and EOGT underlie autosomal recessive inheritance. Data on the frequency and distribution of mutations in large cohorts are currently limited. The purpose of this study was therefore to comprehensively examine the genetic architecture of AOS in an extensive cohort. Molecular diagnostic screening of 194 AOS/ACC/TTLD probands/families was conducted using next-generation and/or capillary sequencing analyses. In total, we identified 63 (likely) pathogenic mutations, comprising 56 distinct and 22 novel mutations, providing a molecular diagnosis in 30% of patients. Taken together with previous reports, these findings bring the total number of reported disease variants to 63, with a diagnostic yield of 36% in familial cases. NOTCH1 is the major contributor, underlying 10% of AOS/ACC/TTLD cases, with DLL4 (6%), DOCK6 (6%), ARHGAP31 (3%), EOGT (3%), and RBPJ (2%) representing additional causality in this cohort. We confirm the relevance of genetic screening across the AOS/ACC/TTLD spectrum, highlighting preliminary but important genotype-phenotype correlations. This cohort offers potential for further gene identification to address missing heritability.Peer reviewe

Heteromorphic variants of chromosome 9

BACKGROUND: Heterochromatic variants of pericentromere of chromosome 9 are reported and discussed since decades concerning their detailed structure and clinical meaning. However, detailed studies are scarce. Thus, here we provide the largest ever done molecular cytogenetic research based on >300 chromosome 9 heteromorphism carriers. RESULTS: In this study, 334 carriers of heterochromatic variants of chromosome 9 were included, being 192 patients from Western Europe and the remainder from Easter-European origin. A 3-color-fluorescence in situ hybridization (FISH) probe-set directed against for 9p12 to 9q13~21.1 (9het-mix) and 8 different locus-specific probes were applied for their characterization. The 9het-mix enables the characterization of 21 of the yet known 24 chromosome 9 heteromorphic patterns. In this study, 17 different variants were detected including five yet unreported; the most frequent were pericentric inversions (49.4%) followed by 9qh-variants (23.9%), variants of 9ph (11.4%), cenh (8.2%), and dicentric- (3.8%) and duplication-variants (3.3%). For reasons of simplicity, a new short nomenclature for the yet reported 24 heteromorphic patterns of chromosome 9 is suggested. Six breakpoints involved in four of the 24 variants could be narrowed down using locus-specific probes. CONCLUSIONS: Based on this largest study ever done in carriers of chromosome 9 heteromorphisms, three of the 24 detailed variants were more frequently observed in Western than in Eastern Europe. Besides, there is no clear evidence that infertility is linked to any of the 24 chromosome 9 heteromorphic variants