The \u3cem\u3eChlamydomonas\u3c/em\u3e Genome Reveals the Evolution of Key Animal and Plant Functions

Chlamydomonas reinhardtii is a unicellular green alga whose lineage diverged from land plants over 1 billion years ago. It is a model system for studying chloroplast-based photosynthesis, as well as the structure, assembly, and function of eukaryotic flagella (cilia), which were inherited from the common ancestor of plants and animals, but lost in land plants. We sequenced the ∼120-megabase nuclear genome of Chlamydomonas and performed comparative phylogenomic analyses, identifying genes encoding uncharacterized proteins that are likely associated with the function and biogenesis of chloroplasts or eukaryotic flagella. Analyses of the Chlamydomonas genome advance our understanding of the ancestral eukaryotic cell, reveal previously unknown genes associated with photosynthetic and flagellar functions, and establish links between ciliopathy and the composition and function of flagella

Annual program review, forest biology, March 26-27, 1998

"March 26-27, 1998."Mass clonal propagation of improved conifers: project F010 / Gerald Pullman ... [et al.] ; Mass clonal propagation of improved conifers - initiation and culture survival ... Gerald Pullman ... [et al.] ; Mass clonal propagation of improved conifers - embryo development improvements based on elemental analysis of female gametophyte, zygotic and somatic embryo tissues / Gerald Pullman ... [et al.] ; Mass clonal propagation of improved conifers-embryo development improvements due to modification of plate size and wrapping materials ... Jerry Pullman ... [et al.] ; Mass clonal propagation of improved conifers: the effects of increasing ABA concentration and extending culture time on quality and quantity of cotyledonary somatic embryos of loblolly pine / Gerald Pullman and Xiaorong Feng ; Mass clonal propagation of improved conifers: optimizing plating methods for somatic embryo yields and automated embryo counting / Gary Peter, Teresa Vales ; Mass clonal propagation of improved conifers: field establishment of somatic embryo derived loblolly pine seedlings / Jerry Pullman ... [et al.] ; Mass clonal propagation of improved conifers - elemental analysis of zygotic female gametophyte and embryo tissues / Gerald Pullman ... [et al.] ; Mass clonal propagation of softwoods: functional analysis of zygotic embryo germination / Gary Peter ... [et al.] ; Mass clonal propagation of softwoods: update - functional analysis of zygotic embryo germination / Gary Peter ... [et al.] ; Mass clonal propagation of improved conifers: the role of the suspensor in early embryo development. I. Differential gene expression in the suspensor of zygotic embryos / John Mackay, Heidi Schindler, Jerry Pullman ; Externally funded research in 1997-1998 supporting F010 / John Cairney ... [et al.] ; Gene expression in loblolly pine embryos: cDNA cloning, high density array and gene expression detection / Nanfei Xu ... [et al.] ; Isolation and characterization of "pinine", a vicilline life seed storage protein from loblolly pine (pinus taeda) / Ranjan Perera ... [et al.] ; Culture cycling and tissue culture decline: pH cycling in loblolly pine suspension cultures and associated gene expression / Michael Sullivan ... [et al.] ; cDNA cloning of mRNAs present early in zygotic embryo development / Vincent Ciavatta, Gerald Pullman and John Cairney ; Combined effects of activated carbon and pH on ionic composition and 2,4-D availability in a tissue culture medium / S.C. Van Winkle, G. S. Pullman ; Mass clonal propagation of genetically improved hardwoods: project F011 / Gary Peter ... [et al.] ; F011 mass clonal propagation of genetically improved hardwoods / Gary Peter ... [et al.] ; F011: mass clonal propagation of genetically improved hardwoods: establishing and improving the zinnia elegans tracheary element differentiation system at IPST / Gary Peter, Karen Floyd, Chris Ricker ; Externally funded research in 1997-1998 supporting F011 / John Cairney ... [ et al.] ; Gene expression in trees: sequences affecting stability/translatability of mRNA / Luis Destefano-Beltran and John Cairney ; Gene regulation in woody plants: identification of a plant enzyme which activates a regulatory peptide / Luis Destefano ... [et al.]

Annual program review, forest biology, October 14-15, 1998

"October 14-15, 1998."Mass clonal propagation of improved conifers: project F010 / Jerry Pullman ... [et al.] ; Mass clonal propagation of improved conifers - initiation / Gerald Pullman ... [et al.] ; Mass clonal propagation of improved conifers: genetic control of initiation and the number of zygotic embryos in the seed / John MacKay ... [et al.] ; Mass clonal propagation of improved conifers: survival of initiated cultures after direct transfer to liquid media / Gary Peter ... [et al.] ; Mass clonal propagation of improved conifers - embryo development improvements based on elemental analysis of female gametophyte, zygotic and somatic embryo tissues / Gerald Pullman, Paul Montello, Xiaorong Feng ; Mass clonal propagation of improved conifers: optimizing the percent of embryos that develop into cotyledonary embryos by altering embryo plating densities and ABA concentration / Teresa Vales, Gary Peter ; Mass clonal propagation of improved conifers: field establishment of somatic embryo derived loblolly pine seedlings / Jerry Pullman, Paul Montello, Mike Cunningham ; Mass clonal propagation of improved conifers: functional anaylsis of pinus taeda zygotic embryo germination: the effect of partial drying and the acquisition of desiccation tolerance / Gary Peter ... [et al.] ; Mass clonal propagation of improved conifers: differential gene expression in the suspensor of zygotic embryos - update / John MacKay ... [et al.] ; Externally funded research in 1998-1999 supporting F010 / John Cairney ... [et al.] ; Gene expression studies with developing embryos: the effect of ABA treatment / Nanfei Xu ... [et al.] ; Early-stage-specific gene expression in loblolly pine zygotic and somatic embryos: cDNA cloning, sequencing and expression analysis / Vincent Ciavatta, Gerald Pullman and John Cairney ; Identification and cloning of cDNAs for novel female-, male- and vegetatively expressed genes / Lin Ge, Gerald Pullman, and John Cairney ; Fundamental biological mechanisms: improved stem growth rates and fiber properties: project F011 / Gary Peter ... [et al.] ; Fundamental biological mechanisms: improved fiber properties and rates of wood cell formation: cellulose biosynthesis / Gary Peter, Chris Ricker ; Externally funded reserach in 1998-1999 supporting F011 / Gary Peter ... [et al.] ; Towards genetic engineering of forest trees with anthraquinones: endogenous anthraquinones in pulpwood trees and the cloning of a key gene for anthraquinone biosynthesis / Huabin Meng ... [et al.] ; Environmental and genetic regulation of microfibril angle in southern pine: determination of the microfibril angle in the S2 layer of pinus taeda using differential interference contrast microscopy: validation of the method / Douglas Benton, Keith Bennett, Gary Peter ; Genetically modified lignin in pine: structure and properties: lignin reactivity in CAD-deficient trees during pulping and bleaching / Donald Dimmel ... [et al.] -- Slide material

The Chlamydomonas Genome Reveals the Evolution of Key Animal and Plant Functions

Chlamydomonas reinhardtii is a unicellular green alga whose lineage diverged from land plants over 1 billion years ago. It is a model system for studying chloroplast-based photosynthesis, as well as the structure, assembly, and function of eukaryotic flagella (cilia), which were inherited from the common ancestor of plants and animals, but lost in land plants. We sequenced the ∼120-megabase nuclear genome of Chlamydomonas and performed comparative phylogenomic analyses, identifying genes encoding uncharacterized proteins that are likely associated with the function and biogenesis of chloroplasts or eukaryotic flagella. Analyses of the Chlamydomonas genome advance our understanding of the ancestral eukaryotic cell, reveal previously unknown genes associated with photosynthetic and flagellar functions, and establish links between ciliopathy and the composition and function of flagella