Interleukin 1 Signaling Is Regulated by Leukemia Inhibitory Factor (LIF) and Is Aberrant in Lif−/− Mouse Uterus

This study addresses the regulation of the interleukin 1 (IL1) system in the murine uterine luminal epithelium (LE) and stroma by leukemia inhibitory factor (LIF). Using RT-PCR we compared expression of Il1a, Il1b, Il1rn, Il1r1, and Il1r2 during the pre- and peri-implantation periods of pregnancy in wild-type (WT) and LIF-null LE and stroma. In WT LE, Il1a transcripts were down-regulated on Day 4 of pregnancy (D4), with renewed expression by the evening of D4 (D4 pm). In Lif−/− LE there was a gradual decrease in expression on D2, and expression became undetectable by D6. Il1b and Il1r1 expression were similar in WT and null mice, but Il1rn expression was almost completely lost during the peri-implantation period in Lif−/− LE. In the stroma, Il1a was sharply down-regulated on D4 and reappeared on D4 pm but was only expressed from D3 to D5 in the null mice. Stromal Il1r1 and Il1r2 were also misregulated. Il1rn showed constitutive expression in null stroma in contrast to the loss of expression on D4 in the WT mouse. In Lif-deficient mice, immunostaining indicated a reduction of endometrial IL1A at the time of implantation and of IL1B in stroma. LE-stromal coculture revealed that LIF stimulated the apical secretion of both IL1A and PTGES2 by LE cells without affecting basal secretion of IL1A and with only a small effect on basal PTGES2 secretion. We conclude that Il1a and Il1rn in LE and Il1a, Il1rn, and Il1r1 in stroma are regulated by LIF, which stimulates apical secretion of IL1A by LE

Asymmetries in ozone depressions between the polar stratospheres following a solar proton event

Ozone depletions in the polar stratosphere during the energetic solar proton event on 4 August 1972 were observed by the backscattered ultraviolet (BUV) experiments on the Nimbus 4 satellite. The observed ozone contents, the ozone depressions and their temporal variations above the 4 mb level exhibited distinct asymmetries between the northern and southern hemispheres. Since the ozone destroying solar particles precipitate rather symmetrically into the two polar atmospheres, due to the geomagnetic dipole field, it is suggested that these asymmetries may be explained in terms of the differences in dynamics between the summer and the winter polar atmospheres. In the summer (northern) hemisphere, the stratospheric and mesospheric ozone depletion and recovery are smooth functions of time due to the preponderance of undistributed orderly flow in this region. On the other hand, the temporal variation of the upper stratospheric ozone in the winter polar atmosphere (southern hemisphere) exhibits large amplitude irregularities. These characteristic differences between the two polar atmospheres are also evident in the vertical distributions of temperatures and winds observed by balloons and rocket soundings

An exploration of how integrative therapists working with clients presenting with complex trauma use EMDR as part of their practice: a grounded theory study

Eye Movement Desensitisation and Reprocessing (EMDR) is widely used for treating complex trauma. Although there is a large evidence base around the efficacy of EMDR, there is little practice-based research into how EMDR therapists work with clients. EMDR therapists come from a range of professional backgrounds and basic EMDR training provides limited guidance on how to integrate it into a therapist’s existing practice. This research therefore aimed to explore how Counselling Psychologists and Psychotherapists, who define their practice as ‘integrative’, use EMDR as part of their practice when working with clients presenting with complex trauma. Two rounds of semi-structured interviews were conducted with nine integratively orientated EMDR therapists who work with clients presenting with complex trauma. Constructivist grounded theory was used to explore how these therapists work with their clients. A theory was constructed from the data, demonstrating the unique and individual nature of the way each therapist works with different clients, but with significant commonalities. Five main categories resulted: ‘Relational Stance’, ‘Working with Developmental History’, ‘Parts Work’, ‘Structure and Process’ and Right-Brain Processes’. The interactions and relationships between the categories demonstrate the fluid, dynamic, co-created and highly individual nature of this way of working and led to the development of the core category ‘Integration – Uniqueness with Commonalities’. EMDR is a relatively new therapy and the ways in which it is researched, trained and practiced continue to evolve. This research has relevance for clinicians working with complex trauma who want to use EMDR as part of their practice. The resulting theory has been developed to support clinicians to work at an appropriate level of complexity when using EMDR with clients presenting with complex trauma as part of an integrative practice. It could also serve to inform training, practice and further research, particularly given the diverse clinical backgrounds of EMDR therapists

A mass action model of a fibroblast growth factor signaling pathway and its simplification

We consider a kinetic law of mass action model for Fibroblast Growth Factor (FGF) signaling, focusing on the induction of the RAS-MAP kinase pathway via GRB2 binding. Our biologically simple model suffers a combinatorial explosion in the number of differential equations required to simulate the system. In addition to numerically solving the full model, we show that it can be accurately simplified. This requires combining matched asymptotics, the quasi-steady state hypothesis, and the fact subsets of the equations decouple asymptotically. Both the full and simplified models reproduce the qualitative dynamics observed experimentally and in previous stochastic models. The simplified model also elucidates both the qualitative features of GRB2 binding and the complex relationship between SHP2 levels, the rate SHP2 induces dephosphorylation and levels of bound GRB2. In addition to providing insight into the important and redundant features of FGF signaling, such work further highlights the usefulness of numerous simplification techniques in the study of mass action models of signal transduction, as also illustrated recently by Borisov and co-workers (Borisov et al. in Biophys. J. 89, 951–66, 2005, Biosystems 83, 152–66, 2006; Kiyatkin et al. in J. Biol. Chem. 281, 19925–9938, 2006). These developments will facilitate the construction of tractable models of FGF signaling, incorporating further biological realism, such as spatial effects or realistic binding stoichiometries, despite a more severe combinatorial explosion associated with the latter

The Impact of Channel Feedback on Opportunistic Relay Selection for Hybrid-ARQ in Wireless Networks

This paper presents a decentralized relay selection protocol for a dense wireless network and describes channel feedback strategies that improve its performance. The proposed selection protocol supports hybrid automatic-repeat-request transmission where relays forward parity information to the destination in the event of a decoding error. Channel feedback is employed for refining the relay selection process and for selecting an appropriate transmission mode in a proposed adaptive modulation transmission framework. An approximation of the throughput of the proposed adaptive modulation strategy is presented, and the dependence of the throughput on system parameters such as the relay contention probability and the adaptive modulation switching point is illustrated via maximization of this approximation. Simulations show that the throughput of the proposed selection strategy is comparable to that yielded by a centralized selection approach that relies on geographic information.Comment: 30 pages, 9 figures, submitted to the IEEE Transactions on Vehicular Technology, revised March 200

Opportunistic Relay Selection with Limited Feedback

It has been shown that a decentralized relay selection protocol based on opportunistic feedback from the relays yields good throughput performance in dense wireless networks. This selection strategy supports a hybrid-ARQ transmission approach where relays forward parity information to the destination in the event of a decoding error. Such an approach, however, suffers a loss compared to centralized strategies that select relays with the best channel gain to the destination. This paper closes the performance gap by adding another level of channel feedback to the decentralized relay selection problem. It is demonstrated that only one additional bit of feedback is necessary for good throughput performance. The performance impact of varying key parameters such as the number of relays and the channel feedback threshold is discussed. An accompanying bit error rate analysis demonstrates the importance of relay selection.Comment: 5 pages, 6 figures, to appear in Proceedings of 2007 IEEE Vehicular Technology Conference-Spring in Dublin, Irelan

A Vaccine Against Group B Streptococcus: Recent Advances

Group B streptococcus (GBS) causes a high burden of neonatal and infant disease globally. Implementing a vaccine for pregnant women is a promising strategy to prevent neonatal and infant GBS disease and has been identified as a priority by the World Health Organisation (WHO). GBS serotype-specific polysaccharide – protein conjugate vaccines are at advanced stages of development, but a large number of participants would be required to undertake Phase III clinical efficacy trials. Efforts are therefore currently focused on establishing serocorrelates of protection in natural immunity studies as an alternative pathway for licensure of a GBS vaccine, followed by Phase IV studies to evaluate safety and effectiveness. Protein vaccines are in earlier stages of development but are highly promising as they might confer protection irrespective of serotype. Further epidemiological, immunological and health economic studies are required to enable the vaccine to reach its target population as soon as possible

Plant structural complexity and mechanical defenses mediate predator-prey interactions in an odonate-bird system.

Habitat-forming species provide refuges for a variety of associating species; these refuges may mediate interactions between species differently depending on the functional traits of the habitat-forming species. We investigated refuge provisioning by plants with different functional traits for dragonfly and damselfly (Odonata: Anisoptera and Zygoptera) nymphs emerging from water bodies to molt into their adult stage. During this period, nymphs experience high levels of predation by birds. On the shores of a small pond, plants with mechanical defenses (e.g., thorns and prickles) and high structural complexity had higher abundances of odonate exuviae than nearby plants which lacked mechanical defenses and exhibited low structural complexity. To disentangle the relative effects of these two potentially important functional traits on nymph emergence-site preference and survival, we conducted two fully crossed factorial field experiments using artificial plants. Nymphs showed a strong preference for artificial plants with high structural complexity and to a lesser extent, mechanical defenses. Both functional traits increased nymph survival but through different mechanisms. We suggest that future investigations attempt to experimentally separate the elements contributing to structural complexity to elucidate the mechanistic underpinnings of refuge provisioning