Optimization, Bioactivity and Composition of Curcama aromatica Salisb. Extraction Oils Extracted by Microwave-assisted and Hydro-distillation

Curcama aromatica Salisb. (C.aromatica) rhizomes were extracted by hydro-distillation (HD) and microwave-assisted extraction (MAE). The response surface method (RSM) and analysis of variance (ANOVA) were carried out to optimize the statistical data of MAE. The extraction oils (Eos) were analyzed by the compositions by GC/MS and were compared to the antioxidant activity, total phenolic contents (TPC), and total flavonoid contents (TFC). The highest yields (dry weight basis) of HD and MAE were 1.96% and 2.22% (w/w) respectively. From the optimization result, the highest yield for MAE was 2.33% at 795 W power, time 44 mins, and a solid/liquid ratio of 100 g/583 ml. The compositions detected from HD and MAE were 41 and 45 contents respectively. Cedrene and copaene were the major compositions, detected in the extraction oils. Cedrene was detected in HD (25.54%) and MAE (23.69%). Copaene was detected in HD (22.97%) and MAE (24.71 %). MAE presented DPPH (%inhibition) at 82.68 ± 0.31 %, TPC at 4227.62 ± 28.72 (mgGAE/g Dry weight), and TFC at 3.92 ± 28.72 (mgRE/g Dry weight) higher than HD. The extraction oils obtained in this study contained many potential compositions, and MAE was an effective method to extract the essential oil from C.aromatica.Keywords: Cedrene; copaene; response surface method; isolation oil; antioxidan