99 research outputs found

    Robotic Wireless Sensor Networks

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    In this chapter, we present a literature survey of an emerging, cutting-edge, and multi-disciplinary field of research at the intersection of Robotics and Wireless Sensor Networks (WSN) which we refer to as Robotic Wireless Sensor Networks (RWSN). We define a RWSN as an autonomous networked multi-robot system that aims to achieve certain sensing goals while meeting and maintaining certain communication performance requirements, through cooperative control, learning and adaptation. While both of the component areas, i.e., Robotics and WSN, are very well-known and well-explored, there exist a whole set of new opportunities and research directions at the intersection of these two fields which are relatively or even completely unexplored. One such example would be the use of a set of robotic routers to set up a temporary communication path between a sender and a receiver that uses the controlled mobility to the advantage of packet routing. We find that there exist only a limited number of articles to be directly categorized as RWSN related works whereas there exist a range of articles in the robotics and the WSN literature that are also relevant to this new field of research. To connect the dots, we first identify the core problems and research trends related to RWSN such as connectivity, localization, routing, and robust flow of information. Next, we classify the existing research on RWSN as well as the relevant state-of-the-arts from robotics and WSN community according to the problems and trends identified in the first step. Lastly, we analyze what is missing in the existing literature, and identify topics that require more research attention in the future

    When Patrolmen Become Corrupted: Monitoring a Graph using Faulty Mobile Robots

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    International audienceA team of k mobile robots is deployed on a weighted graph whose edge weights represent distances. The robots perpetually move along the domain, represented by all points belonging to the graph edges, not exceeding their maximal speed. The robots need to patrol the graph by regularly visiting all points of the domain. In this paper, we consider a team of robots (patrolmen), at most f of which may be unreliable, i.e. they fail to comply with their patrolling duties. What algorithm should be followed so as to minimize the maximum time between successive visits of every edge point by a reliable patrolmen? The corresponding measure of efficiency of patrolling called idleness has been widely accepted in the robotics literature. We extend it to the case of untrusted patrolmen; we denote by Ifk (G) the maximum time that a point of the domain may remain unvisited by reliable patrolmen. The objective is to find patrolling strategies minimizing Ifk (G). We investigate this problem for various classes of graphs. We design optimal algorithms for line segments, which turn out to be surprisingly different from strategies for related patrolling problems proposed in the literature. We then use these results to study the case of general graphs. For Eulerian graphs G, we give an optimal patrolling strategy with idleness Ifk (G) = (f + 1)|E|/k, where |E| is the sum of the lengths of the edges of G. Further, we show the hardness of the problem of computing the idle time for three robots, at most one of which is faulty, by reduction from 3-edge-coloring of cubic graphs — a known NP-hard problem. A byproduct of our proof is the investigation of classes of graphs minimizing idle time (with respect to the total length of edges); an example of such a class is known in the literature under the name of Kotzig graphs

    Excess maternal salt intake produces sex-specific hypertension in offspring: putative roles for kidney and gastrointestinal sodium handling.

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    Hypertension is common and contributes, via cardiovascular disease, towards a large proportion of adult deaths in the Western World. High salt intake leads to high blood pressure, even when occurring prior to birth - a mechanism purported to reside in altered kidney development and later function. Using a combination of in vitro and in vivo approaches we tested whether increased maternal salt intake influences fetal kidney development to render the adult individual more susceptible to salt retention and hypertension. We found that salt-loaded pregnant rat dams were hypernatraemic at day 20 gestation (147±5 vs. 128±5 mmoles/L). Increased extracellular salt impeded murine kidney development in vitro, but had little effect in vivo. Kidneys of the adult offspring had few structural or functional abnormalities, but male and female offspring were hypernatraemic (166±4 vs. 149±2 mmoles/L), with a marked increase in plasma corticosterone (e.g. male offspring; 11.9 [9.3-14.8] vs. 2.8 [2.0-8.3] nmol/L median [IQR]). Furthermore, adult male, but not female, offspring had higher mean arterial blood pressure (effect size, +16 [9-21] mm Hg; mean [95% C.I.]. With no clear indication that the kidneys of salt-exposed offspring retained more sodium per se, we conducted a preliminary investigation of their gastrointestinal electrolyte handling and found increased expression of proximal colon solute carrier family 9 (sodium/hydrogen exchanger), member 3 (SLC9A3) together with altered faecal characteristics and electrolyte handling, relative to control offspring. On the basis of these data we suggest that excess salt exposure, via maternal diet, at a vulnerable period of brain and gut development in the rat neonate lays the foundation for sustained increases in blood pressure later in life. Hence, our evidence further supports the argument that excess dietary salt should be avoided per se, particularly in the range of foods consumed by physiologically immature young

    Mechanisms for the Evolution of a Derived Function in the Ancestral Glucocorticoid Receptor

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    Understanding the genetic, structural, and biophysical mechanisms that caused protein functions to evolve is a central goal of molecular evolutionary studies. Ancestral sequence reconstruction (ASR) offers an experimental approach to these questions. Here we use ASR to shed light on the earliest functions and evolution of the glucocorticoid receptor (GR), a steroid-activated transcription factor that plays a key role in the regulation of vertebrate physiology. Prior work showed that GR and its paralog, the mineralocorticoid receptor (MR), duplicated from a common ancestor roughly 450 million years ago; the ancestral functions were largely conserved in the MR lineage, but the functions of GRs—reduced sensitivity to all hormones and increased selectivity for glucocorticoids—are derived. Although the mechanisms for the evolution of glucocorticoid specificity have been identified, how reduced sensitivity evolved has not yet been studied. Here we report on the reconstruction of the deepest ancestor in the GR lineage (AncGR1) and demonstrate that GR's reduced sensitivity evolved before the acquisition of restricted hormone specificity, shortly after the GR–MR split. Using site-directed mutagenesis, X-ray crystallography, and computational analyses of protein stability to recapitulate and determine the effects of historical mutations, we show that AncGR1's reduced ligand sensitivity evolved primarily due to three key substitutions. Two large-effect mutations weakened hydrogen bonds and van der Waals interactions within the ancestral protein, reducing its stability. The degenerative effect of these two mutations is extremely strong, but a third permissive substitution, which has no apparent effect on function in the ancestral background and is likely to have occurred first, buffered the effects of the destabilizing mutations. Taken together, our results highlight the potentially creative role of substitutions that partially degrade protein structure and function and reinforce the importance of permissive mutations in protein evolution

    Data for: Human Satisfaction as the Ultimate Goal in Ridesharing

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    1. Factors that affect human satisfaction2. A comparison between the simsat algorithm and the optimal cost in terms of satisfaction3. The raw data collected from MTurk users 4. Data from Mturk users that was used to learn the satisfaction functio

    Data for: Human Satisfaction as the Ultimate Goal in Ridesharing

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    1. Factors that affect human satisfaction2. A comparison between the simsat algorithm and the optimal cost in terms of satisfaction3. The raw data collected from MTurk users 4. Data from Mturk users that was used to learn the satisfaction functionTHIS DATASET IS ARCHIVED AT DANS/EASY, BUT NOT ACCESSIBLE HERE. TO VIEW A LIST OF FILES AND ACCESS THE FILES IN THIS DATASET CLICK ON THE DOI-LINK ABOV

    Development of microarray techniques for the study of gene expression in the European eel (Anguilla anguilla) during silvering and migration to seawater

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    The European eel, Anguilla anguilla, has a complex life-cycle involving migrations between the Sargasso Sea and the river systems of Europe and North Africa. The requirement to move across large salinity gradients presents a significant physiological challenge and the developmental stages of the eel are closely linked to these migrations. Microarrays were created to elucidate gene expression changes occurring during; i. The transition from juvenile yellow to the adult sexually maturing, migrating silver eel and; ii. Salinity adaptation during the migration from freshwater to seawater. Groups (n = 6) of freshwater-acclimated yellow or silver eels were transferred to seawater for between 6 hours and 5 months and complementary control groups were transferred to freshwater. Brain, kidney, intestine and gill cDNA libraries were constructed using suppression subtractive hybridisation (SSH) techniques and a novel protocol based on Invitrogen's Gateway cloning system. The latter technique produced a low redundancy (~4 %) EST bank with a wide range of insert sizes (0.5 – 10 kb). Two microarray types were produced; one comprised 5760 clones from the two brain libraries whilst the other was a multi-tissue microarray incorporating 6144 clones from the SSH libraries. Pooled RNA samples were probed against the microarrays to highlight differentially expressed genes. Real-time quantitative PCR (QPCR) was used to validate the observed expression changes of selected genes in the tissues of individual fish. Following yellow to silver transformation of freshwater-adapted eels, the expression of tyrosine 3-mono-oxygenase/tryptophan 5-mono-oxygenase activation protein (14-3-3) and vaccinia related kinase 3 was shown to be consistently elevated. Prolactin expression increased in the brains of silver eels following two-day seawater-acclimation but QPCR analysis revealed high variation amongst freshwater-adapted eels. This is the first eel microarray study and the expression profiles highlighted herein will provide new avenues for research into the sexual development and salinity acclimation of A. anguilla.EThOS - Electronic Theses Online ServiceNatural Environment Research CouncilGBUnited Kingdo
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