3,079 research outputs found
Radiation Damage Studies of Silicon Photomultipliers
We report on the measurement of the radiation hardness of silicon
photomultipliers (SiPMs) manufactured by
Fondazione Bruno Kessler in Italy (1 mm and 6.2 mm), Center of
Perspective Technology and Apparatus in Russia (1 mm and 4.4 mm), and
Hamamatsu Corporation in Japan (1 mm). The SiPMs were irradiated using a
beam of 212 MeV protons at Massachusetts General Hospital, receiving fluences
of up to protons per cm with the SiPMs at operating
voltage. Leakage currents were read continuously during the irradiation. The
delivery of the protons was paused periodically to record scope traces in
response to calibrated light pulses to monitor the gains, photon detection
efficiencies, and dark counts of the SiPMs. The leakage current and dark noise
are found to increase with fluence. Te leakage current is found to be
proportional to the mean square deviation of the noise distribution, indicating
the dark counts are due to increased random individual pixel activation, while
SiPMs remain fully functional as photon detectors. The SiPMs are found to
anneal at room temperature with a reduction in the leakage current by a factor
of 2 in about 100 days.Comment: 35 pages, 25 figure
High-pressure structural investigation of several zircon-type orthovanadates
Room temperature angle-dispersive x-ray diffraction measurements on
zircon-type EuVO4, LuVO4, and ScVO4 were performed up to 27 GPa. In the three
compounds we found evidence of a pressure-induced structural phase
transformation from zircon to a scheelite-type structure. The onset of the
transition is near 8 GPa, but the transition is sluggish and the low- and
high-pressure phases coexist in a pressure range of about 10 GPa. In EuVO4 and
LuVO4 a second transition to a M-fergusonite-type phase was found near 21 GPa.
The equations of state for the zircon and scheelite phases are also determined.
Among the three studied compounds, we found that ScVO4 is less compressible
than EuVO4 and LuVO4, being the most incompressible orthovanadate studied to
date. The sequence of structural transitions and compressibilities are
discussed in comparison with other zircon-type oxides.Comment: 34 pages, 2 Tables, 11 Figure
Suite of simple metrics reveals common movement syndromes across vertebrate taxa
ecause empirical studies of animal movement are most-often site- and species-specific, we lack understanding of the level of consistency in movement patterns across diverse taxa, as well as a framework for quantitatively classifying movement patterns. We aim to address this gap by determining the extent to which statistical signatures of animal movement patterns recur across ecological systems. We assessed a suite of movement metrics derived from GPS trajectories of thirteen marine and terrestrial vertebrate species spanning three taxonomic classes, orders of magnitude in body size, and modes of movement (swimming, flying, walking). Using these metrics, we performed a principal components analysis and cluster analysis to determine if individuals organized into statistically distinct clusters. Finally, to identify and interpret commonalities within clusters, we compared them to computer-simulated idealized movement syndromes representing suites of correlated movement traits observed across taxa (migration, nomadism, territoriality, and central place foraging)
Diarrhea, bacteremia and multiorgan dysfunction due to an extraintestinal pathogenic Escherichia coli strain with enteropathogenic E. coli genes
(c) The Author/sCAUL read and publish agreement 2022fals
Inhomogeneities on the surface of 21 Lutetia, the asteroid target of the Rosetta mission
CONTEXT: In July 2010 the ESA spacecraft Rosetta will fly-by the main belt
asteroid 21 Lutetia. Several observations of this asteroid have been so far
performed, but its surface composition and nature are still a matter of debate.
For long time Lutetia was supposed to have a metallic nature due to its high
IRAS albedo. Later on it has been suggested to have a surface composition
similar to primitive carbonaceous chondrite meteorites, while further
observations proposed a possible genetic link with more evolved enstatite
chondrite meteorites. AIMS: In order to give an important contribution in
solving the conundrum of the nature of Lutetia, in November 2008 we performed
visible spectroscopic observations of this asteroid at the Telescopio Nazionale
Galileo (TNG, La Palma, Spain). METHODS: Thirteen visible spectra have been
acquired at different rotational phases. RESULTS: We confirm the presence of a
narrow spectral feature at about 0.47-0.48 micron already found by Lazzarin et
al. (2009) on the spectra of Lutetia. We also find a spectral feature at about
0.6 micron, detected by Lazzarin et al. (2004) on one of their Lutetia's
spectra. More importantly, our spectra exhibit different spectral slopes
between 0.6 and 0.75 micron and, in particular, we found that up to 20% of the
Lutetia surface could have flatter spectra. CONCLUSIONS: We detected a
variation of the spectral slopes at different rotational phases that could be
interpreted as possibly due to differences in the chemical/mineralogical
composition, as well as to inhomogeneities of the structure of the Lutetia's
surface (e.g., the presence of craters or albedo spots) in the southern
hemisphere.Comment: 3 pages, 2 figures. Accepted for publication in Astronomy and
Astrophysics. Updated on 25 March 2010
Identification of Critical Paraoxonase 1 Residues Involved in High Density Lipoprotein Interaction
Paraoxonase 1 (PON1) is a high density lipoprotein (HDL)-associated protein with atherosclerosis-protective and systemic anti-oxidant functions. We recently showed that PON1, myeloperoxidase, and HDL bind to one another in vivo forming a functional ternary complex (Huang, Y., Wu, Z., Riwanto, M., Gao, S., Levison, B. S., Gu, X., Fu, X., Wagner, M. A., Besler, C., Gerstenecker, G., Zhang, R., Li, X. M., Didonato, A. J., Gogonea, V., Tang, W. H., et al. (2013) J. Clin. Invest. 123, 3815–3828). However, specific residues on PON1 involved in the HDL-PON1 interaction remain unclear. Unambiguous identification of protein residues involved in docking interactions to lipid surfaces poses considerable methodological challenges. Here we describe a new strategy that uses a novel synthetic photoactivatable and click chemistry-taggable phospholipid probe, which, when incorporated into HDL, was used to identify amino acid residues on PON1 that directly interact with the lipoprotein phospholipid surface. Several specific PON1 residues (Leu-9, Tyr-185, and Tyr-293) were identified through covalent cross-links with the lipid probes using affinity isolation coupled to liquid chromatography with on-line tandem mass spectrometry. Based upon the crystal structure for PON1, the identified residues are all localized in relatively close proximity on the surface of PON1, defining a domain that binds to the HDL lipid surface. Site-specific mutagenesis of the identified PON1 residues (Leu-9, Tyr-185, and Tyr-293), coupled with functional studies, reveals their importance in PON1 binding to HDL and both PON1 catalytic activity and stability. Specifically, the residues identified on PON1 provide important structural insights into the PON1-HDL interaction. More generally, the new photoactivatable and affinity-tagged lipid probe developed herein should prove to be a valuable tool for identifying contact sites supporting protein interactions with lipid interfaces such as found on cell membranes or lipoproteins
Identification of Critical Paraoxonase 1 Residues Involved in High Density Lipoprotein Interaction
Paraoxonase 1 (PON1) is a high density lipoprotein (HDL)-associated protein with atherosclerosis-protective and systemic anti-oxidant functions. We recently showed that PON1, myeloperoxidase, and HDL bind to one another in vivo forming a functional ternary complex (Huang, Y., Wu, Z., Riwanto, M., Gao, S., Levison, B. S., Gu, X., Fu, X., Wagner, M. A., Besler, C., Gerstenecker, G., Zhang, R., Li, X. M., Didonato, A. J., Gogonea, V., Tang, W. H., et al. (2013) J. Clin. Invest. 123, 3815–3828). However, specific residues on PON1 involved in the HDL-PON1 interaction remain unclear. Unambiguous identification of protein residues involved in docking interactions to lipid surfaces poses considerable methodological challenges. Here we describe a new strategy that uses a novel synthetic photoactivatable and click chemistry-taggable phospholipid probe, which, when incorporated into HDL, was used to identify amino acid residues on PON1 that directly interact with the lipoprotein phospholipid surface. Several specific PON1 residues (Leu-9, Tyr-185, and Tyr-293) were identified through covalent cross-links with the lipid probes using affinity isolation coupled to liquid chromatography with on-line tandem mass spectrometry. Based upon the crystal structure for PON1, the identified residues are all localized in relatively close proximity on the surface of PON1, defining a domain that binds to the HDL lipid surface. Site-specific mutagenesis of the identified PON1 residues (Leu-9, Tyr-185, and Tyr-293), coupled with functional studies, reveals their importance in PON1 binding to HDL and both PON1 catalytic activity and stability. Specifically, the residues identified on PON1 provide important structural insights into the PON1-HDL interaction. More generally, the new photoactivatable and affinity-tagged lipid probe developed herein should prove to be a valuable tool for identifying contact sites supporting protein interactions with lipid interfaces such as found on cell membranes or lipoproteins
Orthorhombically Mixed s and d Wave Superconductivity and Josephson Tunneling
The effect of orthorhombicity on Josephson tunneling in high T
superconductors such as YBCO is studied for both single crystals and highly
twinned crystals. It is shown that experiments on highly twinned crystals
experimentally determine the symmetry of the superconducting twin boundaries
(which can be either even or odd with respect to a reflection in the twinning
plane). Conversely, Josephson experiments on highly twinned crystals can not
experimentally determine whether the superconductivity is predominantly
-wave or predominantly -wave. The direct experimental determination of
the order-parameter symmetry by Josephson tunneling in YBCO thus comes from the
relatively few experiments which have been carried out on untwinned single
crystals.Comment: 5 pages, RevTeX file, 1 figure available on request
([email protected]
- …