Online taxation

Internet ; Taxation

Implications of financial sector consolidation

Bank mergers ; Consolidation and merger of corporations

Pseudoaurora

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Thermometer exposure

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Pressure of the Vapor of Water

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Thermometer exposure

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Comparative genomic analysis and molecular examination of the diversity of enterotoxigenic Escherichia coli isolates from Chile

Enterotoxigenic Escherichia coli (ETEC) is one of the most common diarrheal pathogens in the low- and middle-income regions of the world, however a systematic examination of the genomic content of isolates from Chile has not yet been undertaken. Whole genome sequencing and comparative analysis of a collection of 125 ETEC isolates from three geographic locations in Chile, allowed the interrogation of phylogenomic groups, sequence types and genes specific to isolates from the different geographic locations. A total of 80.8% (101/125) of the ETEC isolates were identified in E. coli phylogroup A, 15.2% (19/125) in phylogroup B, and 4.0% (5/125) in phylogroup E. The over-representation of genomes in phylogroup A was significantly different from other global ETEC genomic studies. The Chilean ETEC isolates could be further subdivided into sub-clades similar to previously defined global ETEC reference lineages that had conserved multi-locus sequence types and toxin profiles. Comparison of the gene content of the Chilean ETEC identified genes that were unique based on geographic location within Chile, phylogenomic classifications or sequence type. Completion of a limited number of genomes provided insight into the ETEC plasmid content, which is conserved in some phylogenomic groups and not conserved in others. These findings suggest that the Chilean ETEC isolates contain unique virulence factor combinations and genomic content compared to global reference ETEC isolates

Comparative genomics and transcriptomics of Escherichia coli isolates carrying virulence factors of both enteropathogenic and enterotoxigenic E. coli

AbstractEscherichia coli that are capable of causing human disease are often classified into pathogenic variants (pathovars) based on their virulence gene content. However, disease-associated hybrid E. coli, containing unique combinations of multiple canonical virulence factors have also been described. Such was the case of the E. coli O104:H4 outbreak in 2011, which caused significant morbidity and mortality. Among the pathovars of diarrheagenic E. coli that cause significant human disease are the enteropathogenic E. coli (EPEC) and enterotoxigenic E. coli (ETEC). In the current study we use comparative genomics, transcriptomics, and functional studies to characterize isolates that contain virulence factors of both EPEC and ETEC. Based on phylogenomic analysis, these hybrid isolates are more genomically-related to EPEC, but appear to have acquired ETEC virulence genes. Global transcriptional analysis using RNA sequencing, demonstrated that the EPEC and ETEC virulence genes of these hybrid isolates were differentially-expressed under virulence-inducing laboratory conditions, similar to reference isolates. Immunoblot assays further verified that the virulence gene products were produced and that the T3SS effector EspB of EPEC, and heat-labile toxin of ETEC were secreted. These findings document the existence and virulence potential of an E. coli pathovar hybrid that blurs the distinction between E. coli pathovars.</jats:p

Siklus Hidup dan Statistik Demografi Kutukebul Bemisia Tabaci (Gennadius) (Hemiptera: Aleyrodidae) Biotipe B dan Non-B pada Tanaman Cabai (Capsicum Annuum L.)

Kutukebul Bemisia tabaci (Gennadius) biotipe B yang juga dikenal sebagai Bemisia argentifolii (Gennadius) merupakan jenis biotipe yang lebih ganas dalam merusak tanaman dibandingkan dengan biotipe non-B. Saat ini kutukebul B. tabaci biotipe B telah dilaporkan keberadaanya di Indonesia. Informasi dasar, seperti siklus hidup, lama hidup, keperidian, dan kemampuan berkembang biak kutukebul sangat diperlukan sebagai dasar dalam menyusun strategi pengendalian hama kutukebul tersebut. Penelitian bertujuan untuk mempelajari siklus hidup dan statistik demografi kutukebul B. tabaci biotipe B dan non-B pada tanaman cabai. Penelitian dilakukan dengan mengamati perkembangan sejak telur sampai menjadi dewasa di grow chamber yang terkontrol suhu dan pencahayaannya. Pengamatan dilakukan terhadap beberapa aspek biologi dan beberapa parameter statistik demografi. Hasil penelitian menunjukkan bahwa kutukebul B. tabaci biotipe B memiliki beberapa aspek biologi yang berbeda dengan non-B pada tanaman cabai. Siklus hidup kutukebul B. tabaci biotipe B dan non-B masing-masing adalah 33,27 dan 30,86 hari. Kutukebul B. tabaci biotipe B memiliki laju reproduksi bersih (R0) yang hampir sama dengan biotipe non-B, begitu juga rataan masa generasinya. Namun demikian, laju pertambahan intrinsik (r) kutukebul B. tabaci biotipe B 2,5 kali lebih cepat dibandingkan biotipe non-B. Kutukebul B. tabaci biotipe B berlipat ganda populasinya (DT) 2 kali lebih cepat dibandingkan biotipe non-B. Kutukebul B. tabaci biotipe B berpotensi lebih berbahaya dibandingkan dengan biotipe non-B sehingga perlu diwaspadai keberadaannya