The clinical relevance and newsworthiness of NIHR HTA-funded research: a cohort study

ObjectiveTo assess the clinical relevance and newsworthiness of the UK National Institute for Health Research (NIHR) Health Technology Assessment (HTA) Programme funded reports.Study designRetrospective cohort study.SettingThe cohort included 311 NIHR HTA Programme funded reports publishing in HTA in the period 1 January 2007–31 December 2012. The McMaster Online Rating of Evidence (MORE) system independently identified the clinical relevance and newsworthiness of NIHR HTA publications and non-NIHR HTA publications. The MORE system involves over 4000 physicians rating publications on a scale of relevance (the extent to which articles are relevant to practice) and a scale of newsworthiness (the extent to which articles contain news or something clinicians are unlikely to know).Main outcome measuresThe proportion of reports published in HTA meeting MORE inclusion criteria and mean average relevance and newsworthiness ratings were calculated and compared with publications from the same studies publishing outside HTA and non-NIHR HTA funded publications.Results286/311 (92.0%) of NIHR HTA reports were assessed by MORE, of which 192 (67.1%) passed MORE criteria. The average clinical relevance rating for NIHR HTA reports was 5.48, statistically higher than the 5.32 rating for non-NIHR HTA publications (mean difference=0.16, 95% CI 0.04 to 0.29, p=0.01). Average newsworthiness ratings were similar between NIHR HTA reports and non-NIHR HTA publications (4.75 and 4.70, respectively; mean difference=0.05, 95% CI ?0.18 to 0.07, p=0.402). NIHR HTA-funded original research reports were statistically higher for newsworthiness than reviews (5.05 compared with 4.64) (mean difference=0.41, 95% CI 0.18 to 0.64, p=0.001).ConclusionsFunding research of clinical relevance is important in maximising the value of research investment. The NIHR HTA Programme is successful in funding projects that generate outputs of clinical relevance

A Low Dose Caffeine and Carbohydrate Supplement does not Improve Athletic Performance during Volleyball Competition

International Journal of Exercise Science 10(3): 340-353, 2017. Dietary supplements are widely used to enhance sport performance and the combination of carbohydrate and caffeine (CHO+CAF) has yielded particularly high performance gains. Though the effects of a CHO+CAF supplement have been studied in a laboratory environment, little research exists on the effects of supplementation during competition. Therefore, the purpose of this study was to determine the effects of a CHO+CAF supplement on athletic performance in competition. Eight female collegiate volleyball players completed three testing sessions under three different conditions separated by approximately one week each: CHO+CAF supplement, placebo (PBO), and control (CTL) using a randomized, cross-over design. Blood glucose (BG) was assessed prior to supplementation and immediately after set three. The supplement and PBO were administered prior to play and between sets two and three. Following three sets of play, three performance tests were completed: vertical jump (VJ), agility (AGL), and repeated 30-m sprint ability (RSA). While CHO+CAF supplementation significantly increased BG, the performance tests were not different (p\u3e.05) among the testing conditions. These findings suggest that the amount of the supplement used in this study is not beneficial to VJ, AGL, and RSA in female volleyball players. As these performance tests were largely anaerobic and non-glycolytic in nature, the ergogenicity of the supplement may have been underutilized. Additionally, coaches and athletes should not only be aware of what ingredients are in the supplements they choose, but the amount of those ingredients as they may modify the efficacy of the supplement to impact performance

Switch-independent task representations in frontal and parietal cortex

Alternating between two tasks is effortful and impairs performance. Previous fMRI studies have found increased activity in frontoparietal cortex when task switching is required. One possibility is that the additional control demands for switch trials are met by strengthening task representations in the human brain. Alternatively, on switch trials, the residual representation of the previous task might impede the buildup of a neural task representation. This would predict weaker task representations on switch trials, thus also explaining the performance costs. To test this, male and female participants were cued to perform one of two similar tasks, with the task being repeated or switched between successive trials. Multivoxel pattern analysis was used to test which regions encode the tasks and whether this encoding differs between switch and repeat trials. As expected, we found information about task representations in frontal and parietal cortex, but there was no difference in the decoding accuracy of task-related information between switch and repeat trials. Using cross-classification, we found that the frontoparietal cortex encodes tasks using a generalizable spatial pattern in switch and repeat trials. Therefore, task representations in frontal and parietal cortex are largely switch independent. We found no evidence that neural information about task representations in these regions can explain behavioral costs usually associated with task switching

Diffuse HI Disks in Isolated Galaxies

In order to investigate the contribution of diffuse components to their total HI emission, we have obtained high precision HI line flux densities with the 100m Green Bank Telescope for a sample of 100 isolated spiral and irregular galaxies which we have previously observed with the 43m telescope. A comparison of the observed HI line fluxes obtained with the two different telescopes, characterized by half-power beam widths of 9 arcmin and 21 arcmin respectively, exploits a ``beam matching'' technique to yield a statistical determination of the occurrence of diffuse HI components in their disks. A simple model of the HI distribution within a galaxy well describes ~75 % of the sample and accounts for all of the HI line flux density. The remaining galaxies are approximately evenly divided into two categories: ones which appear to possess a significantly more extensive HI distribution than the model predicts, and ones for which the HI distribution is more centrally concentrated than predicted. Examples of both extremes can be found in the literature but little attention has been paid to the centrally concentrated HI systems. Our sample has demonstrated that galaxies do not commonly possess extended regions of low surface brightness HI gas which is not accounted for by our current understanding of the structure of HI disks. Eight HI-rich companions to the target objects are identified, and a set of extragalactic HI line flux density calibrators is presented.Comment: 26 page

A Photometric Method for Quantifying Asymmetries in Disk Galaxies

A photometric method for quantifying deviations from axisymmetry in optical images of disk galaxies is applied to a sample of 32 face-on and nearly face-on spirals. The method involves comparing the relative fluxes contained within trapezoidal sectors arranged symmetrically about the galaxy center of light, excluding the bulge and/or barred regions. Such a method has several advantages over others, especially when quantifying asymmetry in flocculent galaxies. Specifically, the averaging of large regions improves the signal-to-noise in the measurements; the method is not strongly affected by the presence of spiral arms; and it identifies the kinds of asymmetry that are likely to be dynamically important. Application of this "method of sectors" to R-band images of 32 disk galaxies indicates that about 30% of spirals show deviations from axisymmetry at the 5-sigma level.Comment: 17 pages, 2 tables and 6 figures, uses psfig and AAS LaTex; to appear in A

Characterization of Host-Cell Line Specific Glycosylation Profiles of Early Transmitted/Founder HIV-1 gp120 Envelope Proteins

Glycosylation plays an essential role in regulating protein function by modulating biological, structural, and therapeutic properties. However, due to its inherent heterogeneity and diversity, the comprehensive analysis of protein glycosylation remains a challenge. As part of our continuing effort in the analysis of glycosylation profiles of recombinant HIV-1 envelope-based immunogens, we evaluated and compared the host-cell specific glycosylation pattern of recombinant HIV-1 surface glycoprotein, gp120, derived from clade C transmitted/founder virus 1086.C expressed in Chinese hamster ovary (CHO) and human embryonic kidney containing T antigen (293T) cell lines. We used an integrated glycopeptide-based mass mapping workflow that includes a partial deglycosylation step described in our previous study1 with the inclusion of the fragmentation technique, electron transfer dissociation (ETD), to complement collision induced dissociation (CID). The inclusion of ETD facilitated the analysis by providing additional validation for glycopeptide identification and expanding the identified glycopeptides to include coverage of O-linked glycosylation. The site-specific glycosylation analysis shows that the transmitted/founder 1086.C gp120 expressed in CHO and 293T displayed distinct similarities and differences. For N-linked glycosylation, two sites (N386 and N392), in the V4 region were populated with high mannose glycans in the CHO cell-derived 1086.C gp120, while these sites had a mixture of high mannose and processed glycans in the 293T cell-derived 1086.C gp120. Compositional analysis of O-linked glycans revealed that 293T cell-derived 1086.C gp120 consisted of cores 1, 2, and 4 type O-linked glycans while CHO cell-derived 1086.C exclusively consisted of core 1 type O-linked glycans. Overall, glycosylation site occupancy of the CHO and 293T cell-derived 1086.C gp120 show high degree of similarity except for one site at N88 in the C1 region. This site was partially occupied in 293T-gp120 but fully occupied in CHO-gp120. Site-specific glycopeptide analysis of transmitted/founder 1086.C gp120 expressed in CHO cells revealed the presence of phosphorylated glycans while 293T cell produced 1086.C gp120 glycans were not phosphorylated. While the influence of phosphorylated glycans on immunogenicity is unclear, distinguishing host-cell specific variations in glycosylation profiles provides insights into the similarity (or difference) in recombinant vaccine products. While these differences had minimal effect on envelope antigenicity, they may be important in considering immunogenicity and functional capacities of recombinant envelope proteins produced in different expression systems

Investigation of the depolarisation transition in Bi-based relaxor ferroelectrics

The loss of macroscopic polarisation in relaxor ferroelectric (Na0.8K0.2)(1/2)Bi1/2TiO3 ceramics doped with BiZn1/2Ti1/2O3 has been studied by electrical and structural methods. These indicate that the phenomena that are coupled in a displacive phase transition are not necessarily coupled in the depolarisation of Na1/2Bi1/2TiO3-based relaxors and a concept of correlated and uncorrelated switching of dipoles within adjacent unit cells is used to explain this. Second harmonic generation performed on poled ceramics during heating yields values of the freezing temperature and shows a broad temperature range of similar to 100 degrees C across which the structure changes from field-induced ferroelectric to an equilibrium-state ergodic relaxor. Electrical poling at room temperature causes poled regions to increase in size by similar to 2 orders of magnitude. A model illustrating the main steps in thermal depolarisation is described that does not require a phase transition to take place on a unit cell level.open1