Seasonal change of thyroid histomorphological structure and hormone production in yellowfin seabream (Acanthopagrus latus) in the Persian Gulf

Seasonal changes of the thyroid gland structure and hormones secretion was examined in yellowfin seabream (Acanthopagrus latus) in the northwest of Persian Gulf (Musa creek). Thyroid gland composed of follicles scattered around the ventral aorta, near the gills. Follicular cells varied according to secretion of the gland during warm and cold seasons. Thyroid hormones (Triidothyronine [T3] and Thyroxine [T4]) were detected in the fish serum in levels ranged from 4.09-1.30 ng/mL for T3 and from 1.10-0.21 ng/mL for (T4) in the warm and cold seasons, respectively. The results showed that the height of thyroid epithelium and plasma concentration of thyroid hormones (thyroid activity) in A. latus increased significantly during spring and summer. The peak of these factors occurred in midsummer (August). Then, the thyroid activity decreased significantly during autumn and early winter from October to December according to decrease of temperature. T3 and T4 increased significantly from January to April

Role of Mitofusin 2 in the Renal Stress Response

The role of mitofusin 2 (MFN2), a key regulator of mitochondrial morphology and function in the renal stress response is unknown. To assess its role, the MFN2 floxed gene was conditionally deleted in the kidney of mice (MFN2 cKO) by Pax2 promoter driven Cre expression (Pax2Cre). MFN2 cKO caused severe mitochondrial fragmentation in renal epithelial cells that are critical for normal kidney tubular function. However, despite a small (20%) decrease in nephron number, newborn cKO pups had organ or tubular function that did not differ from littermate Cre-negative pups. MFN2 deficiency in proximal tubule epithelial cells in primary culture induced mitochondrial fragmentation but did not significantly alter ATP turnover, maximal mitochondrial oxidative reserve capacity, or the low level of oxygen consumption during cyanide exposure. MFN2 deficiency also did not increase apoptosis of tubule epithelial cells under non-stress conditions. In contrast, metabolic stress caused by ATP depletion exacerbated mitochondrial outer membrane injury and increased apoptosis by 80% in MFN2 deficient vs. control cells. Despite similar stress-induced Bax 6A7 epitope exposure in MFN2 deficient and control cells, MFN2 deficiency significantly increased mitochondrial Bax accumulation and was associated with greater release of both apoptosis inducing factor and cytochrome c. In conclusion, MFN2 deficiency in the kidney causes mitochondrial fragmentation but does not affect kidney or tubular function during development or under non-stress conditions. However, MFN2 deficiency exacerbates renal epithelial cell injury by promoting Bax-mediated mitochondrial outer membrane injury and apoptosis

Network Analysis of Oyster Transcriptome Revealed a Cascade of Cellular Responses during Recovery after Heat Shock

Oysters, as a major group of marine bivalves, can tolerate a wide range of natural and anthropogenic stressors including heat stress. Recent studies have shown that oysters pretreated with heat shock can result in induced heat tolerance. A systematic study of cellular recovery from heat shock may provide insights into the mechanism of acquired thermal tolerance. In this study, we performed the first network analysis of oyster transcriptome by reanalyzing microarray data from a previous study. Network analysis revealed a cascade of cellular responses during oyster recovery after heat shock and identified responsive gene modules and key genes. Our study demonstrates the power of network analysis in a non-model organism with poor gene annotations, which can lead to new discoveries that go beyond the focus on individual genes

Spatial Soil Erosion Risk at the Brimvand Watershed in Kermanshah Province, Iran

Introduction Soil erosion is considered as one of the critical threats to the conservation of water and soil resources. However, until now, its various components, including its spatial changes, have yet to be given due attention. In order to implement soil erosion control and conservation programs in the watershed, it is essential to have basic information leading to know and accurately identify the factors affecting the degradation of soil and water resources. Meanwhile, the CORINE model has been considered as one of the practical models for estimating soil erosion and displaying the spatial distribution of soil erosion with easy and accessible inputs. The CORINE model developed based on the Universal Soil Loss Equation (USLE) was therefore employed in the present study in the GIS environment to determine the potential and actual erosion risks of the Brimvand Watershed in Kermanshah Province, Iran.   Materials and Methods The main associated factors of soil erosion, viz. soil erodibility and erosivity, such as slope, vegetation, depth, texture, and percentage of gravel, were collected, compiled, and ultimately classified in the ArcGIS software. The Fournier index (FI) and FAO-UNEP drought index (DI) were used to prepare the input maps. The Fournier index reflects the performance of the soil erosive agent. In other words, it shows the role of rainfall on soil erosion. The FAO-UNEP drought index shows the simultaneous performance of evaporation and precipitation on soil erosion of a region. The potential soil erosion risk was obtained by combining slope, erodibility, and erosivity layers. In addition, the actual soil erosion risk map was determined by combining the vegetation map and potential soil erosion risk.   Results and Discussion Based on the results of the vegetation distribution in the region, the use of rainfed and abandoned lands had the largest area in the watershed, with an occupation percentage of 65.48%. Furthermore, the rangeland and forest areas, with respective coverage of 29.65 and 4.87%, stood in the second and third priority from the viewpoint of the area. The region has varying slopes, but more importantly, it has a low slope. Soil texture, depth, and gravel content significantly affect the area's erosion. The soil depth in a large part of the watershed, especiallywith a slope of less than 4% is more than 65 cm where it is mainly covered by abandoned and rainfed lands. In the studied area, the depth of the soil decreases with height increase, which indicates that the high slopes of the area are dominantly outcrops. Most of the area has gravel contents between 10 and 40%. The erosion potential of the region is not that much high because of the desired features of the affecting factors. According to the potential soil erosion risk, it was determined that about 65.57, 23.62, and 10.81% of the area were classified as intermediate, low, and high erosion potential, respectively. Further, the actual soil erosion risk was categorized as low, intermediate, and high with respective areal coverage of 53.83, 15.53, and 30.64%. It is therefore implied that the amount of erosion and sediment transfer will increase if the land cover in the watershed is declined.  This indicates that the erosion rate was highest in hilly areas due to lack of vegetation and soil with loamy texture. Accordingly, to curb soil erosion and prevent its associated adverse effects in the Brimvand watershed, it is strongly suggested to use lands based on their capability and potential, maintain the present cover status, and carry out management plans to restore vegetation.   Conclusion Soil erosion remains one of the most critical challenges in watersheds, and its neglect can lead to various problems for the beneficiaries. In light of this, the present study aimed to address this issue by employing the CORINE model to assess potential and actual soil erosion in the Brimvand Watershed of Kermanshah Province, Iran. The research findings reveal the pivotal role of vegetation in mitigating soil erosion. Notably, areas with gentle slopes, which are inherently susceptible to erosion, benefit significantly from vegetation cover, leading to a noticeable reduction in erosion. However, the loss of this protective cover can trigger a rapid increase in soil erosion and subsequent loss of valuable soil resources. By shedding light on the spatial distribution of soil erosion, this study emphasizes the importance of preserving and promoting vegetation in the watershed to ensure its long-term sustainability and safeguard the well-being of those who depend on it. Therefore, land utilization should be planned based on capacity and potential of the land to curb erosion and avoid its detrimental impacts in the Brimvand Watershed. In addition, not only the current cover situation has to be maintained but also the vegetation conditions have to be improved through the implementation of managerial and reclamation plans leading to managing soil erosion

Detection of Affective States From Text and Speech for Real-Time Human–Computer Interaction

Objective: The goal of this work is to develop and test an automated system methodology that can detect emotion from text and speech features. Background: Affective human-computer interaction will be critical for the success of new systems that will be prevalent in the 21st century. Such systems will need to properly deduce human emotional state before they can determine how to best interact with people. Method: Corpora and machine learning classification models are used to train and test a methodology for emotion detection. The methodology uses a stepwise approach to detect sentiment in sentences by first filtering out neutral sentences, then distinguishing among positive, negative, and five emotion classes. Results: Results of the classification between emotion and neutral sentences achieved recall accuracies as high as 77% in the University of Illinois at Urbana-Champaign (UIUC) corpus and 61% in the Louisiana State University medical drama (LSU-MD) corpus for emotion samples. Once neutral sentences were filtered out, the methodology achieved accuracy scores for detecting negative sentences as high as 92.3%. Conclusion: Results of the feature analysis indicate that speech spectral features are better than speech prosodic features for emotion detection. Accumulated sentiment composition text features appear to be very important as well. This work contributes to the study of human communication by providing a better understanding of how language factors help to best convey human emotion and how to best automate this process. Application: Results of this study can be used to develop better automated assistive systems that interpret human language and respond to emotions through 3-D computer graphics. Copyright © 2012, Human Factors and Ergonomics Society

Association of Cystic Fibrosis Genetic Modifiers with Congenital Bilateral Absence of the Vas Deferens

Objective: To investigate whether genetic modifiers of cystic fibrosis (CF) lung disease also predispose to congenital bilateral absence of the vas deferens (CBAVD) in association with cystic fibrosis transmembrane conductance regulator (CFTR) mutations. We tested the hypothesis that polymorphisms of transforming growth factor (TGF)-beta 1 (rs 1982073, rs 1800471) and endothelin receptor type A (EDNRA) (rs 5335, rs 1801708) are associated with the CBAVD phenotype. Design: Genotyping of subjects with clinical CBAVD. Setting: Outpatient and hospital-based clinical evaluation. Patient(s): DNA samples from 80 subjects with CBAVD and 51 healthy male controls from various regions of Europe. This is one of the largest genetic studies of this disease to date. Intervention(s): None. Main Outcome Measure(s): Genotype analysis. Result(s): For single nucleotide polymorphism (SNP) rs 5335, we found increased frequency of the CC genotype among subjects with CBAVD. The difference was significant among Turkish patients versus controls (45.2% vs. 19.4%), and between all cases versus controls (36% vs. 15.7%). No associations between CBAVD penetrance and polymorphisms rs 1982073, rs 1800471, or rs 1801708 were observed. Conclusion(s): Our findings indicate that endothelin receptor type A polymorphism rs 5335 may be associated with CBAVD penetrance. To our knowledge, this is the first study to investigate genetic modifiers relevant to CBAVD. (Fertil Steril (R) 2010; 94: 2122-7. (C) 2010 by American Society for Reproductive Medicine.)Wo

Association of cystic fibrosis genetic modifiers with congenital bilateral absence of the vas deferens

Objective: To investigate whether genetic modifiers of cystic fibrosis (CF) lung disease also predispose to congenital bilateral absence of the vas deferens (CBAVD) in association with cystic fibrosis transmembrane conductance regulator (CFTR) mutations. We tested the hypothesis that polymorphisms of transforming growth factor (TGF)-beta 1 (rs 1982073, rs 1800471) and endothelin receptor type A (EDNRA) (rs 5335, rs 1801708) are associated with the CBAVD phenotype. Design: Genotyping of subjects with clinical CBAVD. Setting: Outpatient and hospital-based clinical evaluation. Patient(s): DNA samples from 80 subjects with CBAVD and 51 healthy male controls from various regions of Europe. This is one of the largest genetic studies of this disease to date. Intervention(s): None. Main Outcome Measure(s): Genotype analysis. Result(s): For single nucleotide polymorphism (SNP) rs 5335, we found increased frequency of the CC genotype among subjects with CBAVD. The difference was significant among Turkish patients versus controls (45.2% vs. 19.4%), and between all cases versus controls (36% vs. 15.7%). No associations between CBAVD penetrance and polymorphisms rs 1982073, rs 1800471, or rs 1801708 were observed. Conclusion(s): Our findings indicate that endothelin receptor type A polymorphism rs 5335 may be associated with CBAVD penetrance. To our knowledge, this is the first study to investigate genetic modifiers relevant to CBAVD. (Fertil Steril (R) 2010; 94: 2122-7. (C) 2010 by American Society for Reproductive Medicine.