Spatio-temporal patterns of recent and future climate extremes in the eastern Mediterranean and Middle East region

Recent and future changes in temperature and precipitation climate extremes are estimated using the Hadley Centre PRECIS ("Providing REgional Climates for Impacts Studies") climate model for the eastern Mediterranean and Middle East region. The area of interest is considered vulnerable to extreme climate events as there is evidence for a temperature rise while precipitation tends to decline, suggesting likely effects on vital socioeconomic sectors in the region. Observations have been obtained for the recent period (1961–1990) and used to evaluate the model output. The spatial distribution of recent temporal trends in temperature indicates strong increasing in minimum temperature over the eastern Balkan Peninsula, Turkey and the Arabian Peninsula. The rate of warming reaches 0.4–0.5 °C decade⁻¹ in a large part of the domain, while warming is expected to be strongest in summer (0.6–0.7 °C decade⁻¹) in the eastern Balkans and western Turkey. The trends in annual and summer maximum temperature are estimated at approximately 0.5 and 0.6 °C decade⁻¹ respectively. Recent estimates do not indicate statistically significant trends in precipitation except for individual sub-regions. Results indicate a future warming trend for the study area over the last 30 years of the 21st century. Trends are estimated to be positive and statistically significant in nearly the entire region. The annual trend patterns for both minimum and maximum temperature show warming rates of approximately 0.4–0.6 °C decade⁻¹, with pronounced warming over the Middle Eastern countries. Summer temperatures reveal a gradual warming (0.5–0.9 °C decade⁻¹) over much of the region. The model projects drying trends by 5–30% in annual precipitation towards the end of the 21st century, with the number of wet days decreasing at the rate of 10–30 days year⁻¹, while heavy precipitation is likely to decrease in the high-elevation areas by 15 days year⁻¹

Mutations in the epidermal growth factor receptor gene are linked to smoking-independent, lung adenocarcinoma

Epidermal growth factor receptor (EGFR) mutations are a potential predictor of the effectiveness of EGFR inhibitors for the treatment of lung cancer. Although EGFR mutations were reported to occur with high frequency in nonsmoking Japanese adenocarcinoma patients, the exact nature has not been fully elucidated. We examined EGFR gene mutations within exons 18–21 and their correlations to clinico-pathological factors and other genetic alterations in tumour specimens from 154 patients who underwent resection for lung cancer at Kyoto University Hospital. Epidermal growth factor receptor mutations were observed in 60 tumours (39.0%), all of which were adenocarcinoma. Among the patients with adenocarcinoma (n=108), EGFR mutations were more frequently observed in nonsmokers than former smokers or current smokers (83.0, 50.0, 15.2%, respectively), in women than men (76.3 vs 34.0%), in tumours with bronchio-alveolar component than those without bronchio-alveolar component (78.9 vs 42.9%), and in well or moderately differentiated tumours than poorly differentiated tumours (72.0, 64.4, 34.2%). No tumours with EGFR mutations had any K-ras codon 12 mutations, which were well-known smoking-related gene mutations. In conclusion, adenocarcinomas with EGFR mutation had a distinctive clinico-pathological feature unrelated to smoking. Epidermal growth factor receptor mutations may play a key role in the development of smoking-independent adenocarcinoma

Downscaling Climate Change Impacts, Socio-Economic Implications and Alternative Adaptation Pathways for Islands and Outermost Regions

This book provides a comprehensive overview of the future scenarios of climate change and management concerns associated with climate change impacts on the blue economy of European islands and outermost regions. The publication collects major findings of the SOCLIMPACT project’s research outcomes, aiming to raise social awareness among policy-makers and industry about climate change consequences at local level, and provide knowledge-based information to support policy design, from local to national level. This comprehensive book will also assist students, scholars and practitioners to understand, conceptualize and effectively and responsibly manage climate change information and applied research. This book provides invaluable material for Blue Growth Management, theory and application, at all levels. This first edition includes up-to-date data, statistics, references, case material and figures of the 12 islands case studies. ¨Downscaling climate change impacts, socio-economic implications and alternative adaptation pathways for Islands and Outermost Regions¨ is a must-read book, given the accessible style and breadth and depth with which the topic is dealt. The book is an up-to-date synthesis of key knowledge on this area, written by a multidisciplinary group of experts on climate and economic modelling, and policy design

Comprehensive DNA Methylation Analysis Reveals a Common Ten-Gene Methylation Signature in Colorectal Adenomas and Carcinomas

Microarray analysis of promoter hypermethylation provides insight into the role and extent of DNA methylation in the development of colorectal cancer (CRC) and may be co-monitored with the appearance of driver mutations. Colonic biopsy samples were obtained endoscopically from 10 normal, 23 adenoma (17 low-grade (LGD) and 6 high-grade dysplasia (HGD)), and 8 ulcerative colitis (UC) patients (4 active and 4 inactive). CRC samples were obtained from 24 patients (17 primary, 7 metastatic (MCRC)), 7 of them with synchronous LGD. Field effects were analyzed in tissues 1 cm (n = 5) and 10 cm (n = 5) from the margin of CRC. Tissue materials were studied for DNA methylation status using a 96 gene panel and for KRAS and BRAF mutations. Expression levels were assayed using whole genomic mRNA arrays. SFRP1 was further examined by immunohistochemistry. HT29 cells were treated with 5-aza-2' deoxycytidine to analyze the reversal possibility of DNA methylation. More than 85% of tumor samples showed hypermethylation in 10 genes (SFRP1, SST, BNC1, MAL, SLIT2, SFRP2, SLIT3, ALDH1A3, TMEFF2, WIF1), whereas the frequency of examined mutations were below 25%. These genes distinguished precancerous and cancerous lesions from inflamed and healthy tissue. The mRNA alterations that might be caused by systematic methylation could be partly reversed by demethylation treatment. Systematic changes in methylation patterns were observed early in CRC carcinogenesis, occuring in precursor lesions and CRC. Thus we conclude that DNA hypermethylation is an early and systematic event in colorectal carcinogenesis, and it could be potentially reversed by systematic demethylation therapy, but it would need more in vitro and in vivo experiments to support this theory

Is crowdsourcing a reliable method for mass data acquisition? The case of COVID-19 spread in greece during spring 2020

We present a GIS-based crowdsourcing application that was launched soon after the first COVID-19 cases had been recorded in Greece, motivated by the need for fast, location-wise data acquisition regarding COVID-19 disease spread during spring 2020, due to limited testing. A single question was posted through a web App, to which the anonymous participants subjectively answered whether or not they had experienced any COVID-19 disease symptoms. Our main goal was to locate geographical areas with increased number of people feeling the symptoms and to determine any temporal changes in the statistics of the survey entries. It was found that the application was rapidly disseminated to the entire Greek territory via social media, having, thus, a great public reception. The higher percentages of participants experiencing symptoms coincided geographically with the highly populated urban areas, having also increased numbers of confirmed cases, while temporal variations were detected that accorded with the restrictions of activities. This application demonstrates that health systems can use crowdsourcing applications that assure anonymity, as an alternative to tracing apps, to identify possible hot spots and to reach and warn the public within a short time interval, increasing at the same time their situational awareness. However, a continuous reminder for participation should be scheduled. © 2020 by the authors. Licensee MDPI, Basel, Switzerland

Earth observation as a facilitator of climate change education in schools: The teachers’ perspectives

Climate change education (CCE) fosters the skills and behavioral patterns of students in regards to climate-related challenges and risks. Despite its importance, the integration of CCE in schools is challenging due to the interdisciplinary nature of climate science and the obstacles and demands of everyday school reality. Here, we examine the case of satellite Remote Sensing (RS) for Earth Observation (EO) as an innovative tool for facilitating CCE. We focus on Greece, a country that, despite being a hot spot for climate change, shows a low level of CCE integration in schools and awareness for EO-based educational resources. Based on interviews with in-service teachers, our research reveals the following: (a) there is a high interest in how satellites depict environmental phenomena; (b) EO is considered an efficient vehicle for promoting CCE in schools because it illustrates climate change impacts most effectively; (c) local natural disasters, such as intense forest fires and floods, are more familiar to students and, thus, preferable for teaching when compared to global issues, such as the greenhouse effect and sea level rise; and (d) educators are in favor of short, hands-on, EO-based activities (also known as “activity-shots”), as the most useful material format for integrating climate change topics in their everyday teaching practice. © 2021 by the authors. Licensee MDPI, Basel, Switzerland

Endothelial nitric oxide synthase gene polymorphisms in recurrent spontaneous abortions

Objective: The risk of miscarriage is enhanced by a variety of genetic and environmental factors. Previous studies indicated an association between endothelial nitric oxide synthase (eNOS) activity, and implantation and maintenance of pregnancy, but it is rather controversial whether polymorphisms of the gene encoding for eNOS are associated with recurrent spontaneous abortions (RSA). The aim of our study was to determine whether the 27 bp intron 4 repeat polymorphism (4VNTR) and a Glu298Asp missense mutation encoded by exon 7 of the eNOS gene are associated with an increased risk for recurrent spontaneous abortions (RSA), in the Greek population. Methods: A total of 126 women who had at least three unexplained spontaneous abortions before 20 weeks of gestation, with the same partner, were included in the study group. The control group consistent of 130 women with at least two live childbirths and without history of abortions. All patients and controls were investigated for the two polymorphisms. To genotype the cohorts we used the PCR-RFLPs method. Results: The observed frequencies of bb, ba, aa genotypes of the VNTR, in intron 4, polymorphism were 0.75, 0.24, 0.01, respectively, for the patient group and 0.73, 0.24, 0.03, respectively, for the control group. The observed frequencies of GG, GT, TT of the Glu298Asp polymorphism were 0.42, 0.45, 0.13, respectively, for the patient group and 0.47, 0.45, 0.08, respectively, for the control group. Statistical analysis of the results indicates no significant difference between the two groups, for both the two studied polymorphisms. Conclusion: Our results do not show any influence of the two polymorphisms, VNTR in intron 4 and Glu298Asp of the eNOS gene, on early pregnancy. © 2008 Springer-Verlag

Knossos, the Little Palace North Project, part one: the Early Greek periods

This article is the first of a series presenting the results of two seasons of excavation (2001 and 2002) at the Little Palace North site, Knossos. The excavation was aimed at the exploration of the exposed earth section directly north of the Little Palace, prior to the construction of a drystone wall which was to protect this section from further erosion. While limited in extent, the excavation provided an opportunity to increase our understanding of the layout of the LM and later town and, more generally, of the complex history of occupation of this area, which spans the period from at least the sixteenth century BC to the fourth century AD.In regard to the early Greek periods, few architectural remains were encountered at the Little Palace North site. Instead, early Greek strata, covering all periods from Subminoan to Orientalizing, yielded evidence for various activities that took place outdoors. These activities included, in one area, the (enigmatic) use of large, clay-lined 'pans' and, in another, metal-working. The results add to our knowledge of the activities at early Greek Knossos and the spatial organization of the settlement.The article offers a full discussion of the stratigraphy of the early Greek levels, the associated pottery, small finds, and archaeobotanical material.Σε αυτό το άρθρο δημοσιεύονται – για πρώτη φορά – τα αποτελέσματα των ανασκαφών (2001 και 2002) στην περιοχή βόρεια του Μικρού Ανακτόρου στην Κνωσό. Σκοπός της ανασκαφής ήταν να ερευνηθεί η αρχαιολογική τομή που βρίσκεται αμέσως βόρεια του Μικρού Ανακτόρου, προτού ανεγερθεί ξερολιθιά με σκοπό να προστατευθεί η τομή από περεταίρω διάβρωση. Παρόλο που ήχαν περιορισμένη σε έκταση, η ανασκαφή μας έδωσε την δυνατότητα να καταλάβουμε καλύτερα την δομή της Υστερομινωικής και υστερότερης πόλης, ιδιαίτερα την πολύπλοκη ιστορία της κατοίκησης της περιοχής από το 160 αιώνα π.Χ. μεχρι και τον 40 αιώνα μ.Χ.Σχετικά με την πρώιμη Ελληνική εποχή, ελάχιστα οικοδομικά λείψανα βρέθηκαν στην περιοχή βόρεια του Μικρού Ανακτόρου. Παρόλα αυτά, τα αρχαιολογικά στρώματα των πρώιμων Ελληνικών χρόνων καλύπτουν από την Υπομινωική μέχρι την Ανατολίζουσα περίοδο, παρουσιάζοντας ενδείξεις για διαφορες δραστηριότητες οι οποίες ελάμβαναν μέρος σε εξωτερικούς χώρους. Οι δραστηριότητες αυτές συμπεριλάμβαναν την αινυγματική χρήση μεγάλων “τηγανιών” με πήλινη επικαλυψη, όπως και μεταλλουργία. Τα αποτελέσματα προσθέτουν στις γνώσεις μας για τις δραστηριότητες στην πρώιμη Ελληνική Κνωσό αλλά και για τη χωροταξία του οικισμού. Το άρθρο αυτό προσφέρει την πλήρη ανάλυση της στρωματογραφίας, της κεραμεικής, των μικροευρημάτων και του αρχαιοβοτανολογικού υλικού των πρώιμων Ελληνικών χρόνων.</jats:p

Development of a Front Identification Scheme for Compiling a Cold Front Climatology of the Mediterranean

The objective of this work is the development of an automated and objective identification scheme of cold fronts in order to produce a comprehensive climatology of Mediterranean cold fronts. The scheme is a modified version of The University of Melbourne Frontal Tracking Scheme (FTS), to take into account the particular characteristics of the Mediterranean fronts. We refer to this new scheme as MedFTS. Sensitivity tests were performed with a number of cold fronts in the Mediterranean using different threshold values of wind-related criteria in order to identify the optimum scheme configuration. This configuration was then applied to a 10-year period, and its skill was assessed against synoptic surface charts using statistic metrics. It was found that the scheme performs well with the dynamic criteria employed and can be successfully applied to cold front identification in the Mediterranean

Changing land use and political economy at neolithic and Bronze Age Knossos, Crete: stable carbon (δ13C) and nitrogen (δ15N) isotope analysis of charred crop grains and faunal bone collagen

Excavations at Knossos have uncovered faunal and archaeobotanical archives spanning the Neolithic and Bronze Age (7 th to 2nd millennia BCE), during which one of Europe’s earliest known farming settlements developed into its first major urban settlement and centre of one of its oldest regional states. Through stable isotope (δ 13C, δ 15N) analysis of seeds and bones (as evidence for the growing conditions of cereal and pulse crops and for the types of forage consumed by livestock), land use and, ultimately, political economy are explored. Changing husbandry conditions overwrite any effects of long-term aridification. Early (7th -6 th millennium BCE) Knossian farmers grew intensively managed cereals and pulses (probably in rotation) that were closely integrated (as manured sources of forage) with livestock. Through the later Neolithic and Bronze Age, settlement growth accompanied more extensive cultivation (eventually with cereals and pulses not in rotation) and greater use of rough graze and, by goats, browse. Pasture on cultivated land remained central, however, to the maintenance of sheep, cattle and pigs. Variable diet of early sheep suggests management at the household level, while thereafter progressive dietary divergence of sheep and goats implies their separate herding. Until the Old Palace phase (early 2nd millennium BCE), urban growth was matched by increasingly extensive and probably distant cultivation and herding, but somewhat more intensive conditions during the New and Final Palace phases (mid-2 nd millennium BCE) perhaps reflect greater reliance on surplus from prime land of previously rival centres that now came under Knossian control