In Silico Analysis of Differentially Expressed Genes in Colorectal Carcinoma

Background: Colorectal carcinoma (CRC) is a primary cause of morbidity and mortality worldwide. Resistance to therapy contributes to poor patient prognosis. The aim of our study is to identify the key proteins and interaction networks implicated in CRC which may serve as possible therapeutic targets and help in overcoming therapy resistance.Methods: The microarray dataset of 58 cases and 62 controls was used to identify Differentially Expressed Genes (DEGs).After constructing protein-protein interaction networks , Cytoscape analysis was done to identify the hub proteins. Based on sub graph centrality, between-ness and degree (≥10), hub proteins were selected for further literature search and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis.Results: A total of 85 up-regulated genes and 95 down-regulated genes of CRC patients were selected based on criteria of P>0.05 and fold change>2.0. The PPI analysis revealed STAT3, HNRNPA2B1, RBM8A, RBM25, ATM, HIST1H2BK, SRSF5 and HNRNPDLas hub proteins. On the basis of criteria set for cytoscape analysis, STAT3 and HNRNPA2B1 were identified as key hub proteins. KEGG pathway analysis revealed vital role of STAT3 in carcinogenesis.Conclusion: In addition of HNRNPA2B1 activation by STAT3, cross talk of STAT3 with other oncogenic signaling pathways signifies its role in colorectal carcinogenesis. Our study highlights thatSTAT3may be a possible therapeutic target which may help in overcoming the dilemma of resistance to drug treatment in advanced cases.Keywords: STAT3, drug resistance, targeted therapy, bioinformatics    

Blindness in children at the Ida Rieu school for the blind and deaf

Objective: To identify the causes of blindness at the Ida Rieu school for the blind and deaf, Karachi, Pakistan.Methods: A cross sectional study was conducted at the Ida Rieu School for the blind and deaf. The data collected from medical record of students was entered into the WHO/PBL eye examination form for children with blindness and low vision.Results: Records of 144 pupils aged between 4-30 years were reviewed, including 67% males and 33% females. One third (31%) children had visual impairment (\u3c 6/18-6/60) and 69% were blind (\u3c 3/60-NPL). The commonest anatomical site was retina (41%) and whole globe (20%). The etiology was unknown in 49% cases. In 33% of cases, the data suggested hereditary cause as the etiology, 40% of cases were preventable and 13% treatable.CONCLUSION: Avoidable causes of blindness were seenin 53% of children, 58% of which were preventable and 19 were treatable

Enhancing societal wellbeing and wealth creation: sewing skills programmed for rural housewives in Sabah

This paper proposes eSisterApparel Program and to be adopted as one of the 1AZAM Malaysian government-sponsored programs, raising the economic and social status of women especially of rural housewives in Sabah, Malaysia. This program has the potential to reduce poverty by providing these housewives with sewing skills, sewing machines, and eSisterApparel Portal in generating new sources of income. Sabah, a state in East Malaysia, has the highest level of poverty and hardcore poverty incidence in Malaysia. Literature reviews and nine (9) blocks of Business Model Canvas (BMC) framework are used as methodology for this paper. As the extent of rural poverty in Sabah is expected to be reduced and societal wellbeing to be enhanced through eSisterApparel Program, an added benefit is the minimization of the rural-urban migration process. This in turn will result in less pressure on government to make provision for additional spending on services such as education, provision of clean drinking water and adequate sewage disposal, housing, and health in urban areas as well as having to deal with a host of problems associated with overgrown cities and towns such as a higher incidence of crime and of shanty towns on the outskirts of these cities

Ecological niche model comparison under different climate scenarios: a case study of Olea spp. in Asia

Ecological niche modeling (and the related species distribution modeling) has been used as a tool with which to assess potential impacts of climate change processes on geographic distributions of species. However, the factors introducing variation into niche modeling outcomes are not well understood: To this end, we used seven algorithms to develop models (Maxent, GARP, BIOCLIM, artificial neural networks, support‐vector machines, climate envelope, and environmental distance) to estimate the potential geographic distribution of olives (Olea europaea sensu lato, including Olea ferruginea) under two climatic data sets (current 2000 and future 2050). Five general circulation models and two representative concentration pathway scenarios were used as predictor variables in future projections of the geographic potential of this species; models were fit at global extents (10′ spatial resolution) but transferred and interpreted for a region of particular interest in Central Asia, which largely avoids problems with truncation of niche estimates. We found marked differences among approaches in predicted distributions and model performance, as well as in the future distributional pattern reconstructed, from one algorithm to another. These general approaches, when model‐to‐model variation is managed appropriately, appear promising in predicting the potential geographic distribution of O. europaea sensu lato and thus can be an effective tool in restoration and conservation planning for wild populations, as well as possible commercial plantations of this species

An Analysis of Adjustment Challenges of Academia Returns Migrants: An Evidence from South Asia

The principal objectives of this research were to analyze their reintegration challenges and to dig out the way to use their social and human capital for getting benefit from the international political economy. Qualitative data were obtained from sixteen highly qualified return migrants in the universities of Faisalabad. Purposive and snowball sampling techniques were adopted to reach the respondents and in-depth interviews with them were conducted with the help of an interview guide. Thematic analysis was applied on the bases of case studies. The return migrants were facing multiple challenges at the institutional, political, and economic levels. They were less paid as they can get better salaries internationally. There is no channel to utilize their human and social capital appropriately that can be beneficial for enhancing the role of the country in the global political economy. The difficulties in the diffusion of new ideas, mismanagement at the professional level, cultural shock, lack of proper infrastructure, and political interference in academic institutions remained challengeable for them. The country should better utilize the capital of the highly qualified return migrants to uplift the political economy of the country

Sports Culture in South Asia: Effects of Modern Bowling Action Rules on Cricket, an Information Technology Perspective

Cricket, a famous game played and watched at its peak in Asia. The game is governed by International Cricket Council (ICC). It is a team sport of eleven players in each side with different roles, of which one is bowler. The bowlers have followed different styles of bowling sinces game has started, of which some declared illegal initially. In our study, we have focused on the cases of illegality reported form South Asian region in post 1990 period a new era of biomechanics based bowling laws started. We have also investigated the updated ICC process for bowlers review and remedy along with biomechanics based labs established worldwide. Finally, we have proposed a new information technology based bowling action evaluation solution, lower in cost and affordable at domestic level. Such study will help bowlers, their coaches and viewers to understand the biomechanics based bowling laws, standings of South Asian bowling after these laws and alternative solutions to rescue it

An overview of the Leucospidae (Hymenoptera, Chalcidoidea) of the Arabian Peninsula with description of a new species

An overview of the family Leucospidae (Hymenoptera, Chalcidoidea) is provided for the leucospid fauna of the Arabian Peninsula. Two genera containing four species are identified based on morphometrics and colour patterns. One species, Leucospis ayezae Usman, Anwar & Ahmad, sp. nov., is described. Leucospis elegans Klug had been previously recorded from Arabia Felix (= Yemen) and is recorded here for the first time from Saudi Arabia. The status of Leucospis aff. namibica from Yemen has been clarified, and this species is placed here in the genus Micrapion Kriechbaumer as M. clavaforme Steffan. An updated key and a map showing the distribution of the family Leucospidae in the Arabian Peninsula is provided. The occurrence and color morphs of all leucospid species that have been recorded so far from the region are briefly discussed

Effects of antiplatelet therapy on stroke risk by brain imaging features of intracerebral haemorrhage and cerebral small vessel diseases: subgroup analyses of the RESTART randomised, open-label trial

Background Findings from the RESTART trial suggest that starting antiplatelet therapy might reduce the risk of recurrent symptomatic intracerebral haemorrhage compared with avoiding antiplatelet therapy. Brain imaging features of intracerebral haemorrhage and cerebral small vessel diseases (such as cerebral microbleeds) are associated with greater risks of recurrent intracerebral haemorrhage. We did subgroup analyses of the RESTART trial to explore whether these brain imaging features modify the effects of antiplatelet therapy

Drug standardization through pharmacognostic approaches and estimation of anticancer potential of chamomile (Matricaria chamomilla L.) using prostate-cancer cell lines : an in-vitro study

Cancer is the major challenge across world and the adenocarcinoma of prostate malignancy is the second most prevalent male cancer. Various medicinal plants are used for the treatment and management of various cancers. Matricaria chamomilla L., is one of the extensively used Unani medicament for the treatment of various type of diseases. In the current study we evaluated most of the parameters prescribed for drug standardization using pharmacognostic approaches. The 2,2 Diphenyl-1-picryl hydrazyl (DPPH) method was utilized for the analysis of antioxidant activity in the flower extracts of M. chamomilla. Moreover, we analyzed the antioxidant and cytotoxic activity of M. chamomilla (Gul-e Babuna) through in-vitro method. DPPH (2,2-diphenyl-1-picryl-hydrazl-hydrate) method was utilized for the analysis of antioxidant activity in the flower extracts of M. chamomilla. CFU and wound healing assay were performed to determine the anti-cancer activity. The results demonstrated that various extracts of M. chamomilla fulfilled most of the parameters of drug standardization and contained good antioxidant and anticancer activities. The ethyl acetate showed higher anticancer activity followed by aqueous, hydroalcoholic, petroleum benzene and methanol by CFU method. Also, the wound healing assay demonstrated that ethyl acetate extract has more significant effect followed by methanol and petroleum benzene extract on prostate cancer cell line (C4-2). The current study concluded that the extract of M. chamomilla flowers could act as good source of natural anti-cancer compounds.CCRUM, New Delhi; Science and Engineering Research Board, Department of Science and Technology, Government of India; SERB research grant; Princess Nourah bint Abdulrahman University, Riyadh, Saudi Arabia; South African Medical Research Council (SAMRC) and the National Research Foundation (NRF).https://www.jcancer.orgam2024Medical OncologySDG-03:Good heatlh and well-bein