Rare Suprasellar Chordoid Meningioma with INI1 Gene Mutation

Background: Chordoid Meningioma is a rare brain tumour characterized genetically by loss of genetic material from chromosome 22q at cytogenetic level resulting in mutation of NF2 gene. Objectives and case report: In the present report, we described a rare case of suprasellar chordoid meningioma, which presented in a 32-year-old-woman. Her only complain was throbbing headache. Neurological examination showed left temporal hemianopia, decreased visual acuity (3/6), and no physical abnormalities related to Castleman syndrome were noted. Cranial magnetic resonance (MR) images demonstrated a 28x15 mm mass in the sellar region, which showed iso-to low intensity that enhanced vividly after gadolinium with upwards displacement of the Optic chiasm. Total surgical excision of the tumour was performed and subsequent histological examination of the tumour showed typical histology pattern of chordoid meningioma grade II according to the WHO classification system of meningiomas. Genomic DNA was extracted and mutation analysis for INI1 gene using primer of exon 4, 5, 7, and 9 showed mutation involving exon 9. DNA sequencing showedheterozygosity C­­­­­­­­­­­­­­­­­­-T mutation in exon 9 of INI1 gene leading to change of amino acid serine to phenylalanine at (codon 63). The details of this case are presented with a review of the literature

Photograph and its role in developing creative thinking skills of children

Our current era is characterized by successive and rapid developments in various fields of human knowledge, and the credit for many of these developments is due to the interest in educational and pedagogical aspects since early childhood, to the extent that interest in early childhood has become a necessary entrance, and a basic beginning for the launch of human development, and the goal is no longer education or upbringing. Preparing children to enroll in school and follow up on the various stages of education, but there is a belief in the necessity of preparing children to be able to face an environment characterized by rapid change and development. Skills, characteristics and abilities in general, and creative abilities in particular. Children are one of the most recipients of creativity and a source of it whenever they have the opportunity to think, especially creative thinking. The child in particular is considered the wealth of the future and the real investment of society, and therefore he must be seen as the focus of the educational process and the focus of operations Education in its various media, and the photograph is one of the sources Or educational stimuli for the child to meditate and unleash his thinking, especially in the early stages of his life, for the photograph has a role, importance and strong impact, as it is said that it sings about a thousand words, as sight is one of the most important and most widely used human senses in acquiring information, as the image addresses all human beings. The image differs from the spoken or written word because it is associated with something concrete, tangible and specific, and the word is linked to something abstract, intangible, and the image differs from the written word in ease of receiving, as the text requires the dismantling of existing relationships Between words, effort, concentration and slowness, while the picture gives the message at once easily. The picture is a powerful educational tool that must be invested. Therefore, there was a need to conduct the current research to reveal the design considerations of photographs to develop the creative thinking skills of a pre-school child and its role in developing thinking skills. child's creativity

Factors associated with patient loyalty in private healthcare sector in Egypt

Purpose – This paper explores the effect of perceived service quality, trust, perceived value and perceived cost on patient satisfaction and loyalty as well as exploring the moderating role of the sociodemographic factors. Design/methodology/approach – The data were gathered from 462 patients via a structured questionnaire, while structural equation modeling was utilized for the analysis. Findings – Results indicated that trust, perceived value and patient satisfaction have important roles in shaping the patient loyalty, while patient satisfaction was found to fully mediate the patient’s perceived service quality. Loyalty relationship was also found to partially mediate the trust–loyalty relationship. Nonetheless, the patient’s satisfaction–loyalty relationship was found to be only moderated by the age factor. Practical implications – Implications are provided to the Egyptian private hospitals in order for them to formulate improvement plans as well as set higher standards of conduct. Originality/value – This original research is the first one, up to the researcher knowledge, that explores the drivers of patient satisfaction in the private hospitals in Egypt

Detection of Helicobacter pylori oipA and dupA genes among dyspeptic patients with chronic gastritis

Helicobacter pylori (H. pylori): is a microbe with wide genetic diversity that infects the stomach of most people in developing countries, leading to several clinical outcomes among different individuals such as gastritis, ulcers, or gastric cancer. Outer inflammatory protein A (oipA) and duodenal ulcer promoting (dupA) genes are among the possible virulence factors which determine the patient outcome. Aim: To detect oipA and dupA genes of H. pylori among dyspeptic Egyptian patients, and to investigate their correlation with the varying degrees of the associated chronic gastritis. Methods: The study enrolled 50 patients with dyspepsia, attending the Gastrointestinal Endoscopy unit of the Gastroenterology and Tropical Departments at Ain Shams University Hospital for upper gastrointestinal endoscopy, in the period between, June and, December 2019. Four antral gastric biopsies were taken from each patient for polymerase chain reaction assay to detect the virulence genes oipA, dupA, and cagA and for histopathological assessment. Results: Forty patients were H. pylori positive by histopathology and PCR. cagA, oipA, and dupA were identified in 6 (15%), 13 (32.5%), 9 (22.5%) of biopsies, respectively. Both cagA and oipA genes were highly significantly associated with increasing the severity of gastritis. Only oipA virulence gene showed a highly significant association with gastroduodenitis. There was a highly significant moderate association between cagA and oipA genes. Conclusion: oipA could be a virulence biomarker that serves a great value in predicting the progress of gastric mucosal damage in patients with chronic gastritis, and targeting antimicrobial therapy in those patients to prevent severe gastroduodenal diseases

Ethylene responsive transcription factor ERF109 retards PCD and improves salt tolerance in plant

Semi-quantitative RT-PCR for tobacco VIGS lines of 13 knocked down TFs induced 2 h post oxalic acid treatment (20 mM) as compared to their WT and VIGS line with empty pTRV2 (V2) plants. Amplicon sizes of different genes and primers used are shown in Additional file 5: Table S3. The Nbactin gene was used as the house-keeping control. Gene codes refer to those indicated in Additional file 3: Table S2. (DOCX 684 kb

Fertiledatepalm – a transdisciplinary collaboration project to ameliorate date palm cultivation via microbial inoculation, organic matter management and mixed cropping using nurse plants

Date palm is an important crop in Morocco, Tunisia and other drylands with a high agricultural, economic and cultural value. Harsh environmental conditions of those areas, further accelerated by climate change and the spread of root diseases, threaten date palm cultivation. To overcome limitations in productivity, high inputs of mineral fertilizers and pesticides are applied. However, these external inputs strongly affect the environment and livelihoods. The project aims at establishing an integrated microbe-assisted fertilization approach, combining the inoculation of native soil microbes, namely arbuscular mycorrhizal fungi (AMF) and plant growth-promoting rhizobacteria (PGPR) during the different date palm growth stages, with adapted agricultural management practices using organic amendments and mixed-cropping in Morocco and Tunisia. As initial step, we established a culture collection of native microbes, isolated from date palm roots and rhizosphere composed of 24 AMF isolates including eight species from six genera, twelve bacterial endophyte isolates composed of Paenibacillus, Mycobacterium, and Achromobacter species and 34 PGPR isolates. Functional characterization of PGPRs revealed that around 50 % can solubilize phosphorus and potassium and between 9 % and 68 % have the ability to produce siderophores, hydrogen cyanid, chitinase, cellulase, amylase and protease. Consortia of microbes were formed and used for inoculations. Experiments under nursery conditions revealed that inoculation with AMF and PGPR combined with compost significantly increased growth of date palms as compared to non-amended controls enabling farmers to decrease the time prior to field transplantation. On-farm trials performed in productive date palm groves have shown that PGPR inoculation with or without mixed-cropping with sorghum as nurse plants significantly increase fruit characteristics such as fruit flesh weight as well as fruit length and diameter for up to 14 % and leaf macronutrient concentrations for up to 200 % while in addition enhancing the mycorrhizal potential of the soil. Our integrated fertilization approach is developed in a participatory approach with key stakeholders in so-called innovation platforms, working at laboratory, on-station and on-farm scale to best tackle farmers’ needs in order to facilitate adoption and implementation

Using Spiritual Connections to Cope With Stress and Anxiety During the COVID-19 Pandemic

During the initial phases of the COVID-19 pandemic, stress and anxiety were pervasive among the masses due to high morbidity and mortality. Besides the fear of coronavirus was also particularly driven by social media. Many people started to look for faith and spiritual connections to gain comfort. The role of spiritual ties and religious beliefs in relation to coping with pandemic stress has acquired the attention of researchers in some parts of the world. This cross-sectional survey aimed at assessing the intensity of stress and anxiety symptoms experienced by people and how much they were alleviated by employing spiritual connections. The study sample comprises 795 respondents with 52% males and 48% females living in Saudi Arabia. The brief online study questionnaire collected data about background variables, anxiety and stress scale from DASS-21, and items from the WHOQOL (SRBP) instrument assessed the use of spiritual beliefs to cope. Multiple regression models were tested to determine the role of spiritual connections after adjusting demographic variables. Results illustrated that after adjusting for gender and age, participants’ anxiety symptoms decreased by (β = −0.27; p = 0.000) units with each unit increase in the use of spiritual connections, and participants’ stress symptoms reduce by (β = −0.36; p = 0.000) units with each unit increase in coping with spirituality. Additionally, females’ risk to experience anxiety and stress symptoms was more than males [(β = 0.88; p = 0.01) and (β = 0.92; p = 0.000)], respectively. An increase in age decreases the likelihood of experiencing anxiety symptoms and stress symptoms by (β = −0.75; p = 0.02) and (β = −0.11; p = 0.000) units, respectively. Findings support the protective role of spiritual connections despite small beta coefficients. The social and cultural context in Saudi Arabia favors deep-rooted connections with spirituality and faith. Our findings support the fact that the reliance on spiritual connections helped older people to deal with exaggerated fear during the initial phase of the COVID-19 pandemic and reduces the risk of experiencing anxiety and stress symptoms. Females and younger participants were relatively vulnerable to developing these symptoms. We discussed these findings considering some recent studies that reported similar relationships and made recommendations for future research

miRNome of Child A hepatocellular carcinoma in Egyptian patients

IntroductionHepatocellular carcinoma (HCC) has different etiologies that contribute to its heterogeneity. In regards to the number of HCC patients, Egypt ranks third in Africa and fifteenth worldwide. Despite significant advancements in HCC diagnosis and treatment, the precise biology of the tumor is still not fully understood, which has a negative impact on patient outcomes.MethodsAdvances in next-generation sequencing (NGS) have increased our knowledge of the molecular complexity of HCC.Results & discussionIn this research, 16 HCC and 6 tumor adjacent tissues (control) of Child A Egyptian patients were successfully profiled for the expression profile of miRNAs by NGS. Forty-one differentially expressed miRNAs (DEMs) were found by differential expression analysis, with 31 being upregulated and 10 being downregulated. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis was then conducted on these differentially expressed miRNAs revealing that Sensitivity and specificity analysis showed that hsa-miR-4488, hsa-miR-3178, and hsa-miR-3182 were unique miRNAs as they are expressed in HCC tissues only. These miRNAs were all highly involved in AMPK signaling pathways. However, hsa-miR-214-3p was expressed in control tissues about eight times higher than in cancer tissues and was most abundant in “pathways in cancer and PI3K-Akt signaling pathway” KEGG terms. As promising HCC diagnostic markers, we here suggest hsa-miR-4488, hsa-miR-3178, hsa-miR-3182, and hsa-miR-214-3p. We further urge future research to confirm these markers' diagnostic and prognostic potential as well as their roles in the pathophysiology of HCC

Signaling mechanisms of a water soluble curcumin derivative in experimental type 1 diabetes with cardiomyopathy

BACKGROUND: Curcumin exhibits anti-diabetic activities, induces heme-oxygenase-1 (HO-1) and is an inhibitor of transcriptional co-activator p300. A novel water soluble curcumin derivative (NCD) has been developed to overcome low invivo bioavailability of curcumin. We evaluated the effect of the NCD on signaling mechanisms involved in cardiomyocyte hypertrophy and studied whether its action is mediated via inducible HO-1. MATERIALS AND METHODS: Rats were divided into controls, controls receiving NCD, diabetic, diabetic receiving NCD, diabetic receiving pure curcumin, diabetic receiving HO inhibitor, zinc protoporphyrin IX (ZnPP IX) and diabetic receiving NCD and ZnPP IX. NCD and curcumin were given orally. After 45 days, cardiac physiologic parameters, plasma glucose, insulin, glycated hemoglobin (GHb), HO-1 gene expression and HO activity in pancreas and cardiac tissues were assessed. Gene expression of p300, atrial natriuretic peptide (ANP) and myocyte enhancer factor 2 (MEF2A and MEF2C) were studied. RESULTS: NCD and curcumin decreased plasma glucose, GHb and increased insulin levels significantly in diabetic rats. This action may be partially mediated by induction of HO-1 gene. HO-1 gene expression and HO activity were significantly increased in diabetic heart and pancreas. Diabetes upregulated the expression of ANP, MEF2A, MEF2C and p300. NCD and curcumin prevented diabetes-induced upregulation of these parameters and improved left ventricular function. The effect of the NCD was better than the same dose of curcumin