Faktor – Faktor yang Mempengaruhi Keberkesanan Amali Kerja Kayu dari Perspektif Pelajar Sekolah Menengah Vokasional di Provinsi Aceh

This study aims to identify factors that influence the effectiveness of practical woodworking and determine the effectiveness of woodworking practical from the perspective of students of secondary vocational schools in the province of Aceh. Factors - factors studied were the workshop equipment, workshop environment. This study was designed to investigate the significant differences concerning factors woodworking practical effectiveness based on demographic factors. A total of 130 respondents were randomly selected from six vocational schools in the province of Aceh. The instrument consists of a set of questionnaire consisting of 49 items and distributed second level students. The results showed there is no significant difference between factors - factors woodworking practical effectiveness based on demographic factors except the income level of the parents and the parents’ workplace. This shows there is a significant effect of the four factors, the effectiveness of practical woodworking, accounting for 60.6% (R2 = 0.606) changes in the variance in effectiveness of hands-on woodworking, and between the two of these factors, the workshop environment is the factor that most influences the effectiveness of the practical work wood, contributing 43.3% (R2 = 0.433) of the variance in effectiveness of woodworking practical

Germination and Plantlet Regeneration of Encapsulated Microshoots of Aromatic Rice ( Oryza sativa

Plant tissues such as somatic embryos, apical shoot tips, axillary shoot buds, embryogenic calli, and protocom-like bodies are potential micropropagules that have been considered for creating synthetic seeds. In the present study, 3–5 mm microshoots of Oryza sativa L. Cv. MRQ 74 were used as explant sources for obtaining synthetic seeds. Microshoots were induced from stem explants on Murashige and Skoog (MS) medium supplemented with 1.5 mg/L benzylaminopurine (BAP). They were encapsulated in 3% (w/v) sodium alginate, 3% sucrose, 0.1 mg/L BAP, and 0.1 mg/L α-Naphthalene acetic acid (NAA). Germination and plantlet regeneration of the encapsulated seeds were tested by culturing them on various germination media. The effect of storage period (15–30 days) was also investigated. The maximum germination and plantlet regeneration (100.0%) were recorded on MS media containing 3% sucrose and 0.8% agar with and without 0.1 mg/L BAP. However, a low germination rate (6.67%) was obtained using top soil as a sowing substrate. The germination rate of the encapsulated microshoots decreased from 93.33% to 3.33% after 30 days of storage at 4°C in the dark. Therefore, further research is being done to improve the germination rate of the synthetic seeds

Effects of NAA and BAP, double-layered media, and light distance on in vitro regeneration of nelumbo nucifera Gaertn. (Lotus), an aquatic edible plant

In vitro direct regeneration of Nelumbo nucifera Gaertn. was successfully achieved from immature explants (yellow plumule) cultured on a solid MS media supplemented with combinations of 0.5 mg/L BAP and 1.5 mg/L NAA which resulted in 16.00 ± 0.30 number of shoots per explant and exhibited a new characteristic of layered multiple shoots, while normal roots formed on the solid MS basal media. The double-layered media gave the highest number of shoots per explant with a ratio of 2 : 1 (liquid to solid) with a mean number of 16.67 ± 0.23 shoots per explant with the formation of primary and secondary roots from immature explants. In the study involving light distance, the tallest shoot (16.67 ± 0.23 mm) obtained from the immature explants was at a light distance of 200 mm from the source of inflorescent light (1000 lux). The plantlets were successfully acclimatized in clay loam soil after 8 months being maintained under in vitro conditions

Hasrat Muda Sdn Bhd / Hishamudin Hasbullah ... [et al.]

As the entrepreneurs, we would like to introduce our business plan and its basic features. The idea of starting the business came to our minds because we observed that there are some imperfections in current products, which is battery manufacturing. In addition to that, for the time being this industry is considered quite new. Hence we have minimal competition over market share. We see that as an opportunity to take a piece of the industry. Because the blooming of transportation industry by the government owned companies like Proton, Perodua and Modenas, as well as government agencies such as police department, and other government agencies, we plan to tie up a tender with the government itself to assure loyal clients. The six of us decided to pull in every inch of resource we can muster to start a manufacturing company. We register the company as a private limited company under the name Hasrat Muda Sdn Bhd. consisting of the following members:

In vitro growth response of explants and micropropagation of Brassica oleraceae L. Var Capitata

Experiments were carried out to establish optimum culture condition and to identify the most responsive explants for regeneration of Brassica oleraceae L. var Capitata through plant tissue culture system. The experiment was induced by manipulating various growth regulators during organogenesis using stems, petioles, leaves and roots as explants. The experimental design of this study was Complete Randomized Design (CRD) method. For shoot regeneration using different concentrations of BAP and NAA, the most responsive explant was stem explants in which MS medium supplemented with 1.5 mg/L BAP and 0.5 mg/L NAA produced 8.400 ± 0.889 shoots per explant. Whereas, for root regeneration using different concentrations of BAP and NAA, the most responsive explant was leaf in which MS media supplemented with 0.5 mg/L BAP and 0.5 mg/L NAA produced 51.133 ± 2.929 roots per explant. This research showed that a morphogenesis study of Brassica oleraceae L. var Capitata through tissue culture system was successfully achieved. This study could lead to the better research and development (R&D) for vegetables in the futur

Detection and quantification of natural pigments extracted from callus of Echinocereus cinerascens

Purpose: This paper aims to study the effect of different organic solvents on the extraction of pigments present in callus cultures of E. cinerascens. Design/methodology/approach: Attempts have been made to extract pigments from callus cultures through tissue culture system as an alternative replacement for conventional plant cultivation as tissue culture provides unlimited supplies of plant samples. Callus of E. cinerascens was induced from stem explant cultured in Murashige and Skoog medium supplemented with combination of 0.5 mg/L 6-benzylaminopurine and 0.5 mg/L α-naphthaleneacetic acid maintained under photoperiod of 16 h light and 8 h dark. Fresh samples of the callus were harvested and dissolved in various types and concentrations of solvents such as 100 per cent acetone, 80 per cent acetone, 95 per cent ethanol, 100 per cent methanol and 90 per cent methanol. Each of the mixtures was directly centrifuged to get clear supernatant containing pigments of interest. The pigments were detected and subsequently quantified via two simple techniques, ultraviolet-visible (UV-Vis) spectrophotometer and thin layer chromatography (TLC). Findings: UV-Vis spectrophotometer detected two families of pigments present in the callus cultures, namely, carotenoids (carotene and xanthophyll) and tetrapyrroles (chlorophyll a and b). Pigment contents in various solvent extractions were estimated using spectroscopic quantification equations established. Through TLC, spots were seen on the plates, and Rf values of each spots were assessed to indicate the possible existence of carotenoids and tetrapyrroles. Originality/value: This preliminary study offers significant finding for further advance research related on natural pigments extracted from E. cinerascens that would provide profits in the future applications, especially in food industry, medicine, agriculture, etc

Effects of NAA and BAP, Double-Layered Media, and Light Distance on In Vitro Regeneration of Nelumbo nucifera Gaertn. (Lotus), an Aquatic Edible Plant

In vitro direct regeneration of Nelumbo nucifera Gaertn. was successfully achieved from immature explants (yellow plumule) cultured on a solid MS media supplemented with combinations of 0.5 mg/L BAP and 1.5 mg/L NAA which resulted in 16.00 ± 0.30 number of shoots per explant and exhibited a new characteristic of layered multiple shoots, while normal roots formed on the solid MS basal media. The double-layered media gave the highest number of shoots per explant with a ratio of 2 : 1 (liquid to solid) with a mean number of 16.67 ± 0.23 shoots per explant with the formation of primary and secondary roots from immature explants. In the study involving light distance, the tallest shoot (16.67 ± 0.23 mm) obtained from the immature explants was at a light distance of 200 mm from the source of inflorescent light (1000 lux). The plantlets were successfully acclimatized in clay loam soil after 8 months being maintained under in vitro conditions

Real-world treatment and outcomes of ALK-positive metastatic non–small cell lung cancer in a southeast Asian country

Purpose: Anaplastic lymphoma kinase (ALK) inhibitors are associated with good overall survival (OS) for ALK-positive metastatic non–small cell lung cancer (NSCLC). However, these treatments can be unavailable or limited by financial constraints in developing countries. Using data from a nationwide lung cancer registry, the present study aimed to identify treatment patterns and clinical outcomes of ALK-positive NSCLC in Malaysia. Methods: This retrospective study examined data of patients with ALK-positive NSCLC from 18 major hospitals (public, private, or university teaching hospitals) throughout Malaysia between January 1, 2015 and December 31, 2020 from the National Cardiovascular and Thoracic Surgical Database (NCTSD). Data on baseline characteristics, treatments, radiological findings, and pathological findings were collected. Overall survival (OS) and time on treatment (TOT) were calculated using the Kaplan–Meier method. Results: There were 1581 NSCLC patients in the NCTSD. Based on ALK gene-rearrangement test results, only 65 patients (4.1%) had ALK-positive advanced NSCLC. Of these 65 patients, 59 received standard-of-care treatment and were included in the analysis. Crizotinib was the most commonly prescribed ALK inhibitor, followed by alectinib and ceritinib. Patients on ALK inhibitors had better median OS (62 months for first-generation inhibitors, not reached at time of analysis for second-generation inhibitors) compared to chemotherapy (27 months), but this was not statistically significant (P=0.835) due to sample-size limitations. Patients who received ALK inhibitors as first-line therapy had significantly longer TOT (median of 11 months for first-generation inhibitors, not reached for second-generation inhibitors at the time of analysis) compared to chemotherapy (median of 2 months; P<0.01). Conclusion: Patients on ALK inhibitors had longer median OS and significantly longer TOT compared to chemotherapy, suggesting long-term benefit. Keywords: ALK inhibitors, chemotherapy, ALK-positive, NSCL