Preliminary Studies on the Use of Monoclonal Antibodies as Probes for Sympathetic Development

The precise structural organization and proper functioning of the adult nervous system depend on the ability of neurones to make highly ordered synaptic connexions. To define molecules involved in the development of these connexions and to study their functional roles, we use primary cultures of dissociated rat sympathetic neurones grown in the virtual absence of non-neuronal cells. These neurones can develop adrenergic or cholinergic properties, depending on the environment in which they are grown. This ability to manipulate neuronal phenotype is being used in an attempt to identify cell surface macromolecules that are important in the development or function of adrenergic and cholinergic properties. We have produced monoclonal antibodies against the surface membranes of these neurones and are in the process of characterizing them. Results are presented on the binding specificity of one of these antibodies and on the effect of two other antibodies on neurotransmitter synthesis, uptake, and release

Dynamic Tobit models

Score-driven models provide a solution to the problem of modelling time series when the observations are subject to censoring and location and/or scale may change over time. The method applies to generalized-t and EGB2 distributions, as well as to the normal distribution. A set of Monte Carlo experiments show that the score-driven model provides good forecasts even when the true model is parameterdriven. The viability of the new models is illustrated by fitting them to data on Chinese stock returns

Differential expression of placental glucocorticoid receptors and growth arrest-specific transcript 5 in term and preterm pregnancies: evidence for involvement of maternal stress.

This article has been made available through the Brunel Open Access Publishing Fund.Pregnancy-specific stress predicts birth outcomes. We hypothesized that there is a maternal stress-GR interaction that can influence fetal birth weight. This study examined the relationship between mothers' stress and attitude towards their pregnancies, placental glucocorticoid receptors (GRs) and growth arrest-specific transcript 5 (GAS5) expression, and the status of GR polymorphism, with their infants' birth weights. GAS5 and GR α were the predominant transcripts in both term and preterm placentas, with GAS5 being primarily localized in the syncytiotrophoblasts. In an attempt to mimic moderate and high stress environment in vitro, BeWo and JEG-3 cytotrophoblast cell lines were treated with 10 nM-1000 nM cortisol. Only expression of GAS5 was significantly upregulated by cortisol in all treatments compared with basal levels, but none of the GRs changed expression significantly. In an attempt to assess a stress versus gene interaction, we studied four GR polymorphisms. In the homozygous group for Tth111I polymorphism, mothers with negative attitudes towards the pregnancy gave birth to infants with significantly lower birth weights compared to women with positive/neutral attitudes. None of the GR splice variants were associated with maternal stress. However, placental GAS5 levels were inversely correlated with maternal stress. This study points towards a potential gene-environment interaction that could be of predictive value for fetal weight.Brunel Open Access Publishing Fun

Effects of shopping addiction on consumer decision-making: Web-based studies in real time

Background and aims: Most research into compulsive buying has focused on its causes: questionnaires have been used to study its association with various factors assumed to be important in its etiology. Few studies have dealt with the effects of being a compulsive buyer on shopping decisions. Also, processes underlying compulsive buying are dynamic but questionnaires give access only to a retrospective view of them from the standpoint of the participant. The aim of the current study was to investigate the decision processes underlying compulsive buying. Methods: Two simulated shopping experiments, each with over 100 participants, were used to compare the decision processes of compulsive shoppers with those of non-compulsive shoppers. This approach allowed us to measure many features of consumer decision-making that are relevant to compulsive shopping. Results: Compulsive shoppers differed from general shoppers in six ways: choice characteristics, searching behavior, overspending, budget-consciousness, effects of credit card availability, and emotional responses to overspending. Conclusions: Results are consistent with the view that compulsive buying, like other behavioral addictions, develops because the cognitive system under-predicts the extent of post-addiction craving produced by emotional and visceral processes

Ephemeral active regions and coronal bright points: A solar maximum Mission 2 guest investigator study

A dominate association of coronal bright points (as seen in He wavelength 10830) was confirmed with the approach and subsequent disappearance of opposite polarity magnetic network. While coronal bright points do occur with ephemeral regions, this association is a factor of 2 to 4 less than with sites of disappearing magnetic flux. The intensity variations seen in He I wavelength 10830 are intermittent and often rapid, varying over the 3 minute time resolution of the data; their bright point counterparts in the C IV wavelength 1548 and 20 cm wavelength show similar, though not always coincident time variations. Ejecta are associated with about 1/3 of the dark points and are evident in the C IV and H alpha data. These results support the idea that the anti-correlation of X-ray bright points with the solar cycle can be explained by the correlation of these coronal emission structures with sites of cancelling flux, indicating that, in some cases, the process of magnetic flux removal results in the release of energy. That the intensity variations are rapid and variable suggests that this process works intermittently