ULF Wave Modeling, Effects, and Applications: Accomplishments, Recent Advances, and Future

Ultra Low Frequency (ULF) waves play important roles in magnetosphere-ionosphere coupling, ring current and radiation belt dynamics, and modulation of higher frequency wave modes and energetic particle precipitation. The &ldquo;ULF wave modeling, effects, and applications&rdquo; (UMEA) focus group - part of the Geospace Environment Modeling effort from 2016 to 2021 - sought to improve understanding of the physics of ULF waves and their specification in geospace models. Through a series of in person and virtual meetings the UMEA focus group brought modelers and experimentalists together to compare ULF wave outputs in different models, plan observation campaigns focused on ULF waves, discuss recent advances in ULF wave research, and identify unresolved ULF wave science questions. This article summarizes major discussion points and accomplishments in the UMEA focus group over the last 6 years, recent advances and their connection to Richard Thorne and Peter Gary&rsquo;s significant contributions to ULF wave research, and the future of ULF wave research. &nbsp;</p

Liquefied petroleum gas or biomass for cooking and effects on birth weight

BACKGROUND: Exposure during pregnancy to household air pollution caused by the burning of solid biomass fuel is associated with adverse health outcomes, including low birth weight. Whether the replacement of a biomass cookstove with a liquefied petroleum gas (LPG) cookstove would result in an increase in birth weight is unclear. METHODS: We performed a randomized, controlled trial involving pregnant women (18 to <35 years of age and at 9 to <20 weeks’ gestation as confirmed on ultrasonography) in Guatemala, India, Peru, and Rwanda. The women were assigned in a 1:1 ratio to use a free LPG cookstove and fuel (intervention group) or to continue using a biomass cookstove (control group). Birth weight, one of four prespecified primary outcomes, was the primary outcome for this report; data for the other three outcomes are not yet available. Birth weight was measured within 24 hours after birth. In addition, 24-hour personal exposures to fine particulate matter (particles with a diameter of ≤2.5 μm [PM2.5]), black carbon, and carbon monoxide were measured at baseline and twice during pregnancy. RESULTS: A total of 3200 women underwent randomization; 1593 were assigned to the intervention group, and 1607 to the control group. Uptake of the intervention was nearly complete, with traditional biomass cookstoves being used at a median rate of less than 1 day per month. After randomization, the median 24-hour personal exposure to fine particulate matter was 23.9 μg per cubic meter in the intervention group and 70.7 μg per cubic meter in the control group. Among 3061 live births, a valid birth weight was available for 94.9% of the infants born to women in the intervention group and for 92.7% of infants born to those in the control group. The mean (±SD) birth weight was 2921±474.3 g in the intervention group and 2898±467.9 g in the control group, for an adjusted mean difference of 19.6 g (95% confidence interval, −10.1 to 49.2). CONCLUSIONS: The birth weight of infants did not differ significantly between those born to women who used LPG cookstoves and those born to women who used biomass cookstoves. (Funded by the National Institutes of Health and the Bill and Melinda Gates Foundation; HAPIN ClinicalTrials.gov number, NCT02944682. opens in new tab.

Conjugation of Organoruthenium(II) 3-(1H-Benzimidazol-2-yl)pyrazolo[3,4-b]pyridines and Indolo[3,2-d]benzazepines to Recombinant Human Serum Albumin: a Strategy To Enhance Cytotoxicity in Cancer Cells

Five organoruthenium complexes [RuCl(η6-arene)(L)]Cl with a modified arene ligand, namely, 4-formylphenoxyacetyl-η6-benzylamide, and L = 3-(1H-benzimidazol-2-yl)-1H-pyrazolo[3,4-b]pyridines or indolo[3,2-d]benzazepines were synthesized and conjugated to recombinant human serum albumin in order to improve their drug targeting and delivery to cancer cells, and a marked increase in cytotoxicity was observed

Conjugation of a Ru(II) Arene Complex to Neomycin or to Guanidinoneomycin Leads to Compounds with Differential Cytotoxicities and Accumulation between Cancer and Normal Cells

A straightforward methodology for the synthesis of conjugates between a cytotoxic organometallic ruthenium(II) complex and amino- and guanidinoglycosides, as potential RNA-targeted anticancer compounds, is described. Under microwave irradiation, the imidazole ligand incorporated on the aminoglycoside moiety (neamine or neomycin) was found to replace one triphenylphosphine ligand from the ruthenium precursor [(η6-p-cym)RuCl(PPh3)2]+, allowing the assembly of the target conjugates. The guanidinylated analogue was easily prepared from the neomycin-ruthenium conjugate by reaction with N,N′-di-Boc-N″-triflylguanidine, a powerful guanidinylating reagent that was compatible with the integrity of the metal complex. All conjugates were purified by semipreparative high-performance liquid chromatography (HPLC) and characterized by electrospray ionization (ESI) and matrix-assisted laser desorption-ionization time-of-flight (MALDI-TOF) mass spectrometry (MS) and NMR spectroscopy. The cytotoxicity of the compounds was tested in MCF-7 (breast) and DU-145 (prostate) human cancer cells, as well as in the normal HEK293 (Human Embryonic Kidney) cell line, revealing a dependence on the nature of the glycoside moiety and the type of cell (cancer or healthy). Indeed, the neomycin-ruthenium conjugate (2) displayed moderate antiproliferative activity in both cancer cell lines (IC50 ≈ 80 μM), whereas the neamine conjugate (4) was inactive (IC50 ≈ 200 μM). However, the guanidinylated analogue of the neomycin-ruthenium conjugate (3) required much lower concentrations than the parent conjugate for equal effect (IC50 = 7.17 μM in DU-145 and IC50 = 11.33 μM in MCF-7). Although the same ranking in antiproliferative activity was found in the nontumorigenic cell line (3 2 > 4), IC50 values indicate that aminoglycoside-containing conjugates are about 2-fold more cytotoxic in normal cells (e.g., IC50 = 49.4 μM for 2) than in cancer cells, whereas an opposite tendency was found with the guanidinylated conjugate, since its cytotoxicity in the normal cell line (IC50 = 12.75 μM for 3) was similar or even lower than that found in MCF-7 and DU-145 cancer cell lines, respectively. Cell uptake studies performed by ICP-MS with conjugates 2 and 3 revealed that guanidinylation of the neomycin moiety had a positive effect on accumulation (about 3-fold higher in DU-145 and 4-fold higher in HEK293), which correlates well with the higher antiproliferative activity of 3. Interestingly, despite the slightly higher accumulation in the normal cell than in the cancer cell line (about 1.4-fold), guanidinoneomycin-ruthenium conjugate (3) was more cytotoxic to cancer cells (about 1.8-fold), whereas the opposite tendency applied for neomycin-ruthenium conjugate (2). Such differences in cytotoxic activity and cellular accumulation between cancer and normal cells open the way to the creation of more selective, less toxic anticancer metallodrugs by conjugating cytotoxic metal-based complexes such as ruthenium(II) arene derivatives to guanidinoglycosides

Crucial future observations and directions for unveiling magnetopause dynamics and their geospace impacts

The dynamics of Earth’s magnetopause, driven by several different external/internal physical processes, plays a major role in the geospace energy budget. Given magnetopause motion couples across many space plasma regions, numerous forms of observations may provide valuable information in understanding these dynamics and their impacts. In-situ multi-point spacecraft measurements measure the local plasma environment, dynamics and processes; with upcoming swarms providing the possibility of improved spatiotemporal reconstruction of dynamical phenomena, and multi-mission conjunctions advancing understanding of the “mesoscale” coupling across the geospace “system of systems.” Soft X-ray imaging of the magnetopause should enable boundary motion to be directly remote sensed for the first time. Indirect remote sensing capabilities might be enabled through the field-aligned currents associated with disturbances to the magnetopause; by harnessing data from satellite mega-constellations in low-Earth orbit, and taking advantage of upgraded auroral imaging and ionospheric radar technology. Finally, increased numbers of closely-spaced ground magnetometers in both hemispheres may help discriminate between high-latitude processes in what has previously been a “zone of confusion.” Bringing together these multiple modes of observations for studying magnetopause dynamics is crucial. These may also be aided by advanced data processing techniques, such as physics-based inversions and machine learning methods, along with comparisons to increasingly sophisticated geospace assimilative models and simulations

Comparison of ULF Wave Indices from Conjugate Magnetometer Arrays in Antarctica and Greenland

ULF (Ultra-Low-Frequency) wave index, characterizing the level of geomagnetic field fluctuations in the frequency band of most intense Pc5 pulsations (1.7–6.0 MHz), was introduced earlier for the analysis of space weather. This global hourly index was produced from available 1-min magnetometer data, but from stations in the Northern hemisphere only. If ULF wave powers in conjugate hemispheres were strongly asymmetric, an index derived from one hemisphere data only may be misleading to characterize the global magnetospheric ULF activity. Therefore, it is important to examine how strong is the interhemispheric asymmetry of the ULF power. This issue is relevant to important problem: how strongly does the ionosphere control interhemispheric properties of Pc5 pulsations? To resolve this problem, we use data from the conjugate Antarctica–Greenland autonomous magnetometer arrays along the 40° magnetic meridian. Regional ULF wave power indices and latitudes with a maximal ULF power have been calculated from pairs of conjugate stations in both hemispheres. Examination of daytime ULF indices shows that even under contrasting ionospheric conductances during winter/summer seasons the ULF wave powers in both hemispheres are nearly the same and differ not more that 10%. This result indicates that the excitation of geomagnetic pulsations in the Pc5 band corresponds the current generator regime and justify the usage of the Northern hemisphere index for characterization of an entire magnetosphere