Bacterioplankton dynamics within a large anthropogenically impacted urban estuary

© 2016 Jeffries, Schmitz Fontes, Harrison, Van-Dongen-Vogels, Eyre, Ralph and Seymour. The abundant and diverse microorganisms that inhabit aquatic systems are both determinants and indicators of aquatic health, providing essential ecosystem services such as nutrient cycling but also causing harmful blooms and disease in impacted habitats. Estuaries are among the most urbanized coastal ecosystems and as a consequence experience substantial environmental pressures, providing ideal systems to study the influence of anthropogenic inputs on microbial ecology. Here we use the highly urbanized Sydney Harbor, Australia, as a model system to investigate shifts in microbial community composition and function along natural and anthopogenic physicochemical gradients, driven by stormwater inflows, tidal flushing and the input of contaminants and both naturally and anthropogenically derived nutrients. Using a combination of amplicon sequencing of the 16S rRNA gene and shotgun metagenomics, we observed strong patterns in microbial biogeography across the estuary during two periods: one of high and another of low rainfall. These patterns were driven by shifts in nutrient concentration and dissolved oxygen leading to a partitioning of microbial community composition in different areas of the harbor with different nutrient regimes. Patterns in bacterial composition were related to shifts in the abundance of Rhodobacteraceae, Flavobacteriaceae, Microbacteriaceae, Halomonadaceae, Acidomicrobiales, and Synechococcus, coupled to an enrichment of total microbial metabolic pathways including phosphorus and nitrogen metabolism, sulfate reduction, virulence, and the degradation of hydrocarbons. Additionally, community beta-diversity was partitioned between the two sampling periods. This potentially reflected the influence of shifting allochtonous nutrient inputs on microbial communities and highlighted the temporally dynamic nature of the system. Combined, our results provide insights into the simultaneous influence of natural and anthropogenic drivers on the structure and function of microbial communities within a highly urbanized aquatic ecosystem

Communicative Openness within Adoptive Families: The Role of Photos and Visual Communication

HCI has a long history of designing to support family life, despite this little is known about the unique needs of adoptive families. Though work has explored the role of photos, and photo sharing in the family home, this does not take account of the essential role of photos in supporting the well-being of adopted children. We report on a Research through Design (RtD) project investigating how adoptive parents communicate life story, which is essential to a child's sense of identity. In this pictorial we show the role of photos and visual communication in enabling core aspects of communicative openness between parent and child, as derived from interviews with adopters about their child's life story book. We conclude by discussing the need for visual guidelines and tools to support a more nuanced use of photos and illustrations

Outcomes following oesophagectomy in patients with oesophageal cancer: a secondary analysis of the ICNARC Case Mix Programme Database

Introduction: This report describes the case mix and outcomes of patients with oesophageal cancer admitted to adult critical care units following elective oesophageal surgery in England, Wales and Northern Ireland. Methods: Admissions to critical care following elective oesophageal surgery for malignancy were identified using data from the Intensive Care National Audit and Research Centre (ICNARC) Case Mix Programme Database. Information on admissions between December 1995 and September 2007 were extracted and the association between in-hospital mortality and patient characteristics on admission to critical care was assessed using multiple logistic regression analysis. The performance of three prognostic models (Simplified Acute Physiology Score (SAPS) II, Acute Physiology and Chronic Health Evaluation (APACHE) II and the ICNARC physiology score) was also evaluated. Results: Between 1995 and 2007, there were 7227 admissions to 181 critical care units following oesophageal surgery for malignancy. Overall mortality in critical care was 4.4% and in-hospital mortality was 11%, although both declined steadily over time. Eight hundred and seventy-three (12.2%) patients were readmitted to critical care, most commonly for respiratory complications (49%) and surgical complications (25%). Readmitted patients had a critical care unit mortality of 24.7% and in-hospital mortality of 33.9%. Overall in-hospital mortality was associated with patient age, and various physiological measurements on admission to critical care (partial pressure of arterial oxygen (PaO2):fraction of inspired oxygen (FiO2) ratio, lowest arterial pH, mechanical ventilation, serum albumin, urea and creatinine). The three prognostic models evaluated performed poorly in measures of discrimination, calibration and goodness of fit. Conclusions: Surgery for oesophageal malignancy continues to be associated with significant morbidity and mortality. Age and organ dysfunction in the early postoperative period are associated with an increased risk of death. Postoperative serum albumin is confirmed as an additional prognostic factor. More work is required to determine how this knowledge may improve clinical management

Proposing a holistic framework for the assessment and management of manufacturing complexity through data-centric and human-centric approaches

A multiplicity of factors including technological innovations, dynamic operating environments, and globalisation are all believed to contribute towards the ever-increasing complexity of manufacturing systems. Although complexity is necessary to meet functional needs, it is important to assess and monitor it to reduce life-cycle costs by simplifying designs and minimising failure modes. This research paper identifies and describes two key industrially relevant methods for assessing complexity, namely a data-centric approach using the information theoretic method and a human-centric approach based on surveys and questionnaires. The paper goes onto describe the benefits and shortcomings of each and contributes to the body of knowledge by proposing a holistic framework that combines both assessment methods

Spatially Resolving a Starburst Galaxy at Hard X-ray Energies: NuSTAR, Chandra, AND VLBA Observations of NGC 253

Prior to the launch of NuSTAR, it was not feasible to spatially resolve the hard (E > 10 keV) emission from galaxies beyond the Local Group. The combined NuSTAR dataset, comprised of three ~165 ks observations, allows spatial characterization of the hard X-ray emission in the galaxy NGC 253 for the first time. As a follow up to our initial study of its nuclear region, we present the first results concerning the full galaxy from simultaneous NuSTAR, Chandra, and VLBA monitoring of the local starburst galaxy NGC 253. Above ~10 keV, nearly all the emission is concentrated within 100" of the galactic center, produced almost exclusively by three nuclear sources, an off-nuclear ultraluminous X-ray source (ULX), and a pulsar candidate that we identify for the first time in these observations. We detect 21 distinct sources in energy bands up to 25 keV, mostly consisting of intermediate state black hole X-ray binaries. The global X-ray emission of the galaxy - dominated by the off-nuclear ULX and nuclear sources, which are also likely ULXs - falls steeply (photon index >~ 3) above 10 keV, consistent with other NuSTAR-observed ULXs, and no significant excess above the background is detected at E > 40 keV. We report upper limits on diffuse inverse Compton emission for a range of spatial models. For the most extended morphologies considered, these hard X-ray constraints disfavor a dominant inverse Compton component to explain the {\gamma}-ray emission detected with Fermi and H.E.S.S. If NGC 253 is typical of starburst galaxies at higher redshift, their contribution to the E > 10 keV cosmic X-ray background is < 1%.Comment: 20 pages, 14 figures, accepted for publication in Ap

The development of metaphorical language comprehension in typical development and in Williams syndrome

The domain of figurative language comprehension was used to probe the developmental relation between language and cognition in typically developing individuals and individuals with Williams syndrome. Extending the work of Vosniadou and Ortony, the emergence of nonliteral similarity and category knowledge was investigated in 117 typically developing children between 4 and 12 years of age, 19 typically developing adults, 15 children with Williams syndrome between 5 and 12 years of age, and 8 adults with Williams syndrome. Participants were required to complete similarity and categorization statements by selecting one of two words (e.g., either “The sun is like ___” or “The sun is the same kind of thing as ___”) with word pairs formed from items that were literally, perceptually, or functionally similar to the target word or else anomalous (e.g., moon, orange, oven, or chair, respectively). Results indicated that individuals with Williams syndrome may access different, less abstract knowledge in figurative language comparisons despite the relatively strong verbal abilities found in this disorder

Measurements of Bc+ production and mass with the Bc+ -> J/Ψπ+ decay

Measurements of B-c(+) production and mass are performed with the decay mode B-c(+) -> J/psi pi(+) using 0.37 fb(-1) of data collected in pp collisions at root s = 7 TeV by the LHCb experiment. The ratio of the production cross section times branching fraction between the B-c(+) -> J/psi pi(+) and the B+ -> J/psi K+ decays is measured to be (0.68 +/- 0.10(stat) +/- 0.03(syst) +/- 0.05(lifetime))% for B-c(+) and B+ mesons with transverse momenta p(T) > 4 GeV/c and pseudorapidities 2.5 < eta < 4.5. The B-c(+) mass is directly measured to be 6273.7 +/- 1.3(stat) +/- 1.6(syst) MeV/c(2), and the measured mass difference with respect to the B+ meson is M(B-c(+)) - M(B+) = 994.6 +/- 1.3(stat) +/- 0.6(syst) MeV/c(2)

Measurement of the forward energy flow in pp collisions at √s=7 TeV

The energy flow created in pp collisions at √s = 7 TeV is studied within the pseudorapidity range 1.9 < η < 4.9 with data collected by the LHCb experiment. The measurements are performed for inclusive minimum-bias interactions, hard scattering processes and events with an enhanced or suppressed diffractive contribution. The results are compared to predictions given by PYTHIA-based and cosmic-ray event generators, which provide different models of soft hadronic interactions

Measurements of the branching fractions of B+→ppK+ decays

The branching fractions of the decay B+ → pp̄K+ for different intermediate states are measured using data, corresponding to an integrated luminosity of 1.0 fb-1, collected by the LHCb experiment. The total branching fraction, its charmless component Mpp̄ < 2.85 GeV/c2 and the branching fractions via the resonant cc̄ states η c(1S) and ψ(2S) relative to the decay via a J/ψ intermediate state are [Equation not available: see fulltext.] Upper limits on the B + branching fractions into the η c(2S) meson and into the charmonium-like states X(3872) and X(3915) are also obtained