Myxozoan pathogens in cultured Malaysian fishes. I. Myxozoan infections of the sutchi catfish Pangasius hypophthalmus in freshwater cage cultures

Cage-cultured sutchi catfish Pangasius hypophthalmus (Sauvage, 1878), a favourite food fish in Southeast Asia, proved to be infected by 6 myxozoan species. Three species belonged to the genus Hennegoides (H. berlandi, H. malayensis, and H. pangasii), 1 to Henneguya (H. shariffi) and 2 to Myxobolus (M. baskai, and M. pangasii). Five myxozoans infected the gills and 1 was found on the spleen. Myxozoans infecting the gills were characterised by a specific site selection. H. shariffi sp. n. and H. berlandi sp. n. formed plasmodia in the multi-layered epithelium of the gill filaments. Of the 2 vascular species H. pangasii sp. n. developed in the gin arteries, while M. baskai sp. n. infected the capillary network of the gill lamellae. Plasmodia of H. malayensis sp. n. were found inside the cartilaginous gill rays of the filaments. Large plasmodia of M. pangasii sp. n. were located in a groove of the spleen but they affected only the serosa layer covering the spleen

Myxozoan pathogens in cultured Malaysian fishes. II. Myxozoan infections of redtail catfish Hemibagrus nemurus in freshwater cage cultures

Cage-cultured Asian redtail catfish Hemibagrus nemurus (Valenciennes, 1840), a popular food fish in Southeast Asia, proved to be infected by 3 myxozoan species. All the 3 species belonged to the genus Henneguya: 2 were identified as H. mystusia Sarkar, 1985 and H. hemibagri Tchang et Ma, 1993, while the other was described as H, basifilamentalis sp. n. All plasmodia were found in the gills and were characterised by a specific site selection. H. mystusia formed plasmodia in the multi-layered epithelium between the gill lamellae and in the non-lamellar edge of the gill filaments, while H. hemibagri developed in the capillary network of the lamellae. H. basifilamentalis sp. n. had large oval plasmodia located deep among the filaments just above the gill arch

A pilot study examining garment severance damage caused by a trained sharp-weapon user

The pilot study summarized in this paper aimed to raise awareness of a gap that exists in the forensic textile science literature about damage caused to clothing by trained sharp-weapon users. A male trained in the Filipino martial arts discipline of Eskrima performed attack techniques on a physical model of a male torso covered with a 97% cotton/3% elastane knitted T-shirt, that is, a garment commonly worn by males. Fabric severance appearance created by three different, but commonly available, knives was evaluated. High-speed video was used to capture each attack. After each attack the resulting damage to the garment was assessed. This pilot study highlighted differences in severances associated with weapon selection, that is, not all knives resulted in similar patterns of textile damage. In addition, a mixture of stab and slash severances were observed. The findings demonstrated the possible misinterpretation of textile damage under these circumstances compared to damage patterns reported in the existing forensic textile science literature for more commonly occurring knife attacks (i.e. stabbings)

Gamma-Ray Burst Spectral Features: Interpretation as X-ray Emission From A Photoionized Plasma

Numerous reports have been made of features, either in emission or absorption, in the 10 - 1000 keV spectra of some gamma-ray bursts. Originally interpreted in the context of Galactic neutron star models as cyclotron line emission and $e^+ - e^-$ annihilation features, the recent demonstration that the majority of GRBs lie at cosmological distances make these explanations unlikely. In this letter, we adopt a relativistic fireball model for cosmological GRBs in which dense, metal rich blobs or filaments of plasma are entrained in the relativistic outflow. In the context of this model, we investigate the conditions under which broadband features, similar to those detected, can be observed. We find a limited region of parameter space capable of reproducing the observed GRB spectra. Finally, we discuss possible constraints further high-energy spectral observations could place on fireball model parameters.Comment: Accepted for publication in Astrophysical Journal Letters Four pages, 2 figure

Zapotec Language Activism And Talking Dictionaries

Online dictionaries have become a key tool for some indigenous communities to promote and preserve their languages, often in collaboration with linguists. They can provide a pathway for crossing the digital divide and for establishing a first-ever presence on the internet. Many questions around digital lexicography have been explored, although primarily in relation to large and well-resourced languages. Lexical projects on small and under-resourced languages can provide an opportunity to examine these questions from a different perspective and to raise new questions (Mosel, 2011). In this paper, linguists, technical experts, and Zapotec language activists, who have worked together in Mexico and the United States to create a multimedia platform to showcase and preserve lexical, cultural, and environmental knowledge, share their experience and insight in creating trilingual online Talking Dictionaries in several Zapotec languages. These dictionaries sit opposite from big data mining and illustrate the value of dictionary projects based on small corpora, including having the flexibility to make design decisions to maximize community impact and elevate the status of marginalized languages

Conjugative transfer frequencies of mef(A)-containing Tn1207.3 to macrolide-susceptible Streptococcus pyogenes belonging to different emm types

The aim of this study was to examine the gene transfer potential of mef(A)-containing Tn120.3 to macrolide-susceptible Streptococcus pyogenes belonging to different emm types. Using the filter mating technique, Tn1207.3 was transferred by conjugation to 23 macrolide-susceptible recipients representing 11 emm types. PCR analysis confirmed the presence of the mef(A) gene and the comEC junction regions of the Tn1207.3 insertion in resultant transconjugants. Significant variation was found in the transfer frequency of Tn1207.3 to different Strep. pyogenes strains, and this phenomenon may contribute to the differences in mef(A) frequency observed among clinical isolates. Significance and Impact of the Study: The spread of antimicrobial resistance among pathogenic bacteria is an important problem, but the mechanisms of horizontal transfer between strains and species are often poorly understood. For instance, little is known on how macrolide resistance spreads between strains of the human pathogen Strep. pyogenes and why certain strains more commonly display resistance than others. Here, we show that Strep. pyogenes strains vary greatly in their ability to acquire a transposon encoding macrolide resistance by horizontal gene transfer in vitro. These data provide a novel insight into the transfer of antibiotic resistance between bacterial strains and offer an explanation for the differences in the frequency of resistance determinates and resistance seen among clinical isolates. © 2014 The Authors Letters in Applied Microbiology

A NuSTAR observation of the fast symbiotic nova V745 Sco in outburst

The fast recurrent nova V745 Sco was observed in the 3-79 keV X-rays band with NuSTAR 10 days after the optical discovery. The measured X-ray emission is consistent with a collisionally ionized optically thin plasma at temperature of about 2.7 keV. A prominent iron line observed at 6.7 keV does not require enhanced iron in the ejecta. We attribute the X-ray flux to shocked circumstellar material. No X-ray emission was observed at energies above 20 keV, and the flux in the 3-20 keV range was about 1.6 $\times$ 10$^{-11}$ erg cm$^{-2}$ s$^{-1}$. The emission measure indicates an average electron density of order of 10$^7$ cm$^{-3}$. The X-ray flux in the 0.3-10 keV band almost simultaneously measured with Swift was about 40 times larger, mainly due to the luminous central supersoft source emitting at energy below 1 keV. The fact that the NuSTAR spectrum cannot be fitted with a power law, and the lack of hard X-ray emission, allow us to rule out Comptonized gamma rays, and to place an upper limit of the order of 10$^{-11}$ erg cm$^{-2}$ s$^{-1}$ on the gamma-ray flux of the nova on the tenth day of the outburst.Comment: in press in Monthly Notices of the Royal Astronomical Society, 201

NGC 1068: No change in the mid-IR torus structure despite X-ray variability

Context. Recent NuSTAR observations revealed a somewhat unexpected increase in the X-ray flux of the nucleus of NGC 1068. We expect the infrared emission of the dusty torus to react on the intrinsic changes of the accretion disk. Aims. We aim to investigate the origin of the X-ray variation by investigating the response of the mid-infrared environment. Methods. We obtained single-aperture and interferometric mid-infrared measurements and directly compared the measurements observed before and immediately after the X-ray variations. The average correlated and single-aperture fluxes as well as the differential phases were directly compared to detect a possible change in the structure of the nuclear emission on scales of $\sim$ 2 pc. Results. The flux densities and differential phases of the observations before and during the X-ray variation show no significant change over a period of ten years. Possible minor variations in the infrared emission are $\lesssim$ 8 %. Conclusions. Our results suggest that the mid-infrared environment of NGC 1068 has remained unchanged for a decade. The recent transient change in the X-rays did not cause a significant variation in the infrared emission. This independent study supports previous conclusions that stated that the X-ray variation detected by NuSTAR observations is due to X-ray emission piercing through a patchy section of the dusty region.Comment: 6 pages, 5 figures, 3 tables. Accepted for publication on A&