The Cytoscan (TM) model E-II, a new reflectance microscope for intravital microscopy: Comparison with the standard fluorescence method

The Cytoscan(TM) Model E-II (Cytometrics Inc., Philadelphia, Pa., USA) is a newly developed instrument which functions as an intravital microscope and is small and easily portable. Through the use of orthogonal polarization spectral (OPS) imaging, the Cytoscan Model E-II delivers images of the microcirculation which are comparable to those achieved with intravital fluorescence videomicroscopy (IFM), but without the use of fluorescent dyes. The purpose of this study was to validate the Cytoscan Model E-II instrument against IFM. The experiments were carried out on striated muscle in the dorsal skinfold chamber of the awake Syrian hamster. The following parameters were measured in identical regions of interest in the same animal under baseline conditions and 0.5 and 2 h after a 4-hour period of pressure-induced ischemia: arteriolar diameter, venular diameter and venular red blood cell velocity. Bland-Altman plots showed good agreement between the two techniques for venular red blood cell velocity. As expected, arteriolar and venular diameters as measured by the Cytoscan were on average 5 mum smaller than the values from IFM, since the Cytoscan measures the red blood cell column width and IFM measures luminal diameter. Thus, OPS imaging can be used to make valid measurements of microvascular diameter and red blood cell velocity in tissues. Copyright (C) 2000 S. Karger AG, Basel

User's operating procedures. Volume 3: Projects directorate information programs

A review of the user's operating procedures for the scout project automatic data system, called SPADS is presented. SPADS is the results of the past seven years of software development on a prime mini-computer. SPADS was developed as a single entry, multiple cross-reference data management and information retrieval system for the automation of Project office tasks, including engineering, financial, managerial, and clerical support. This volume, three of three, provides the instructions to operate the projects directorate information programs in data retrieval and file maintenance via the user friendly menu drivers

Evaluation of moire techniques for wind tunnel metrology

The development of a moire technique suitable for the analysis of object deflections in a cryogenically cooled, transonic wind tunnel is described. The operating environment for the wind tunnel has a temperature range of 77 to 3390 k, pressure to 91390 Kgs/sq m, and noise to 150 dB SPL. Efforts were made to accomplish the following: to demonstrate projection moire as it would be used to study structural deflections; to use optical processing to multiply the sensitivity of the moire; and to investigate a system design based on the requirements of the wind tunnel geometry

User's operating procedures. Volume 2: Scout project financial analysis program

A review is presented of the user's operating procedures for the Scout Project Automatic Data system, called SPADS. SPADS is the result of the past seven years of software development on a Prime mini-computer located at the Scout Project Office, NASA Langley Research Center, Hampton, Virginia. SPADS was developed as a single entry, multiple cross-reference data management and information retrieval system for the automation of Project office tasks, including engineering, financial, managerial, and clerical support. This volume, two (2) of three (3), provides the instructions to operate the Scout Project Financial Analysis program in data retrieval and file maintenance via the user friendly menu drivers

On the global circulation and the hurricane system of the Jovian atmosphere

An argument is made to support the proposal that Jupiter's red spot and the white and brown ovals are hurricanes or cyclones. Against the background of a convectively unstable troposphere, the general condition exists for the formation of hurricanes. The energy Jupiter emits must be transported upwards through the troposphere. If that transport is accelerated by the prevailing upward motions in the solar driven multicellular meridional circulation, eastward jets develop such as observed in the l region. But if that vertical transport is impeded by the prevailing downward motions in the meridional circulation, the atmosphere reacts and tends to maintain the process through the development of hurricanes. Dynamically induced by solar differential heating, an ordered latitudinal structure with alternating stability and instability is impressed on the troposphere to form alternating zonal strata where hurricanes are forbidden and permitted, respectively

Tools made of ice facilitate forming of soft, sticky materials

Tools made of ice facilitate the forming or shaping of materials that are soft and sticky in the uncured state. The low-temperature of the ice slows the curing of the material, extending the working time available before setup. Handling problems are eliminated because the material does not adhere to the tool, and the melting ice serves as a lubricant

Statistical derivation of design criteria for liquid rocket combustion instability Final report

Statistical correlation between engine design and combustion stability in liquid propellant rocket engine

A structure in the early Universe at z 1.3 that exceeds the homogeneity scale of the R-W concordance cosmology

A Large Quasar Group (LQG) of particularly large size and high membership has been identified in the DR7QSO catalogue of the Sloan Digital Sky Survey. It has characteristic size (volume^1/3) ~ 500 Mpc (proper size, present epoch), longest dimension ~ 1240 Mpc, membership of 73 quasars, and mean redshift = 1.27. In terms of both size and membership it is the most extreme LQG found in the DR7QSO catalogue for the redshift range 1.0 = 1.28, which is itself one of the more extreme examples. Their boundaries approach to within ~ 2 deg (~ 140 Mpc projected). This new, huge LQG appears to be the largest structure currently known in the early universe. Its size suggests incompatibility with the Yadav et al. scale of homogeneity for the concordance cosmology, and thus challenges the assumption of the cosmological principle

Deep ROSAT-HRI observations of the NGC 1399/NGC 1404 region: morphology and structure of the X-ray halo

We present the analysis of a deep (167 ks) ROSAT HRI observation of the cD galaxy NGC 1399 in the Fornax cluster. Using both HRI and, at larger radii, archival PSPC data, we find that the radial behavior of the X-ray surface brightness profile is not consistent with a simple Beta model and suggests instead three distinct components. We use a multi-component bidimensional model to study in detail these three components that we identify respectively with the cooling flow region, the galactic and the cluster halo. From these data we derive a binding mass distribution in agreement with that suggested by optical dynamical indicators, with an inner core dominated by luminous matter and an extended dark halo differently distributed on galactic and cluster scales. The HRI data and a preliminary analysis of Chandra public data, allow us to detect significant density fluctuations in the halo. We discuss possible non-equilibrium scenarios to explain the hot halo structure, including tidal interactions with neighboring galaxies, ram stripping from the intra-cluster medium and merging events. In the innermost region of NGC 1399, the comparison between the X-ray and radio emission suggests that the radio emitting plasma is displacing and producing shocks in the hot X-ray emitting gas. We found that the NGC 1404 halo is well represented by a single symmetric Beta model and follows the stellar light profile within the inner 8 kpc. The mass distribution is similar to the `central' component of the NGC 1399 halo. At larger radii ram pressure stripping from the intra-cluster medium produces strong asymmetries in the gas distribution. Finally we discuss the properties of the point source population finding evidence of correlation between the source excess and NGC 1399.Comment: 34 pages in aastex5.0 format, including 28 B&W and 4 color figures. Uses LaTex packages: subfigure, lscape and psfig. Accepted for publication in ApJ. High resolution version can be found at: http://www.na.astro.it/~paolillo/publications.htm

Comparison of regional blood flow values measured by radioactive and fluorescent microspheres

Fluorescent microspheres (FM) have become an attractive alternative to radioactive microspheres (RM) for the measurement of regional blood flow (RBF). The aim of the present study was to investigate the comparability of both methods by measuring RBF with FM and RM. Eight anaesthetised pigs received simultaneous, left atrial injections of FM and RM with a diameter of 15 mum at six different time points. Blood reference samples were collected from the descending aorta. RBF was determined in tissue samples of the myocardium, spleen and kidneys of all 8 animals. After radioactivity of the tissue samples was determined, the samples were processed automatically for measuring fluorescence using a recently developed filter device (SPU). RBF was calculated with both the isotope and spectrometric data of both methods for each sample resulting in a total of 10,512 blood flow values. The comparison of the RBF values yielded high linear correlation (mean r(2) = 0.95 +/- 0.03 to 0.97 +/- 0.02) and excellent agreement (bias 5.4-6.7%, precision 9.9-16.5%) of both methods. Our results indicate the validity of MS and of the automated tissue processing technique by means of the SPU. Copyright (C) 2002 S. Karger AG, Basel