87 research outputs found
Biosignal Generation and Latent Variable Analysis with Recurrent Generative Adversarial Networks
The effectiveness of biosignal generation and data augmentation with
biosignal generative models based on generative adversarial networks (GANs),
which are a type of deep learning technique, was demonstrated in our previous
paper. GAN-based generative models only learn the projection between a random
distribution as input data and the distribution of training data.Therefore, the
relationship between input and generated data is unclear, and the
characteristics of the data generated from this model cannot be controlled.
This study proposes a method for generating time-series data based on GANs and
explores their ability to generate biosignals with certain classes and
characteristics. Moreover, in the proposed method, latent variables are
analyzed using canonical correlation analysis (CCA) to represent the
relationship between input and generated data as canonical loadings. Using
these loadings, we can control the characteristics of the data generated by the
proposed method. The influence of class labels on generated data is analyzed by
feeding the data interpolated between two class labels into the generator of
the proposed GANs. The CCA of the latent variables is shown to be an effective
method of controlling the generated data characteristics. We are able to model
the distribution of the time-series data without requiring domain-dependent
knowledge using the proposed method. Furthermore, it is possible to control the
characteristics of these data by analyzing the model trained using the proposed
method. To the best of our knowledge, this work is the first to generate
biosignals using GANs while controlling the characteristics of the generated
data
Radiological assessments and clinical results of intra-articular osteotomy for traumatic osteoarthritis of the ankle
博士(医学)福島県立医科大
Cluster Entropy: Active Domain Adaptation in Pathological Image Segmentation
The domain shift in pathological segmentation is an important problem, where
a network trained by a source domain (collected at a specific hospital) does
not work well in the target domain (from different hospitals) due to the
different image features. Due to the problems of class imbalance and different
class prior of pathology, typical unsupervised domain adaptation methods do not
work well by aligning the distribution of source domain and target domain. In
this paper, we propose a cluster entropy for selecting an effective whole slide
image (WSI) that is used for semi-supervised domain adaptation. This approach
can measure how the image features of the WSI cover the entire distribution of
the target domain by calculating the entropy of each cluster and can
significantly improve the performance of domain adaptation. Our approach
achieved competitive results against the prior arts on datasets collected from
two hospitals.Comment: Accepted by IEEE ISBI'2
A new quantitative index in the diagnosis of Parkinson syndrome by dopamine transporter single photon emission computed tomography
Objective
Dopamine transporter single-photon emission computed tomography (DAT SPECT) has been widely used to diagnose Parkinson syndrome. Using the standardized uptake value (SUV) of DAT SPECT, we propose “functional dopamine transporter volume (f-DTV)” as a new quantitative index to evaluate the three-dimensional volume of functional dopamine transporters and assess its diagnostic ability in differentiating dopaminergic neurodegenerative diseases (dNDD) from non-dNDD.
Methods
Seventy-nine patients were enrolled (42 dNDD, 37 non-dNDD; 38 men; age, 24–88 years). We analyzed seven quantitative indices. The specific binding ratio (SBR) was calculated using a program specialized for DAT SPECT (SBR_Bolt). The SUVmax, SUVpeak, and SUVmean were calculated using a quantification program for bone SPECT. SBR_SUV was calculated by dividing striatal SUVmean by the average of background SUVmean. The cutoff value of the active dopamine transporter level was examined using three methods (threshold of 40% of SUVmax, SUV 2, and SUV 3) to calculate the active dopamine transporter volume (ADV). The f-DTV was calculated by multiplying ADV and SUVmean. We assessed the correlations between SBR_Bolt and SBR_SUV, and compared the mean value of each index between the dNDD and non-dNDD groups. The abilities of SBR_Bolt, SBR_SUV, SUVmax, SUVpeak, SUVmean, ADV, and f-DTV in differentiating dNDD from non-dNDD were determined by the area under the receiver operating curve (AUC) generated by the receiver operating characteristics analysis.
Results
The SBR_Bolt and SBR_SUV highly correlated each other (r = 0.71). The cutoff value of the active dopamine transporter level was determined as SUV 3. All seven quantitative indices showed lower values in the dNDD group than in the non-dNDD group, and the difference between the two groups was statistically significant (p<0.05). Sensitivity, specificity, and AUC of f-DTV were slightly lower than those of SBR_Bolt (71%, 79%, and 0.81, respectively, for f-DTV, and 81%, 84%, 0.88, respectively, for SBR_Bolt). The difference in AUC between f-DTV and SBR_Bolt was not statistically significant.
Conclusions
This study demonstrates the utility of f-DTV as a novel quantitative index for evaluating the three-dimensional volume of functional dopamine transporters, and that f-DTV has almost the same diagnostic ability to differentiate dNDD from non-dNDD using DAT SPECT
Discovery of anti-inflammatory physiological peptides that promote tissue repair by reinforcing epithelial barrier formation
上皮バリアを形成するペプチドJIPの発見 --JIPは上皮組織修復に貢献する--. 京都大学プレスリリース. 2021-11-18.Epithelial barriers that prevent dehydration and pathogen invasion are established by tight junctions (TJs), and their disruption leads to various inflammatory diseases and tissue destruction. However, a therapeutic strategy to overcome TJ disruption in diseases has not been established because of the lack of clinically applicable TJ-inducing molecules. Here, we found TJ-inducing peptides (JIPs) in mice and humans that corresponded to 35 to 42 residue peptides of the C terminus of alpha 1-antitrypsin (A1AT), an acute-phase anti-inflammatory protein. JIPs were inserted into the plasma membrane of epithelial cells, which promoted TJ formation by directly activating the heterotrimeric G protein G13. In a mouse intestinal epithelial injury model established by dextran sodium sulfate, mouse or human JIP administration restored TJ integrity and strongly prevented colitis. Our study has revealed TJ-inducing anti-inflammatory physiological peptides that play a critical role in tissue repair and proposes a previously unidentified therapeutic strategy for TJ-disrupted diseases
A tryptophan-rich breakfast and exposure to light with low color temperature at night improve sleep and salivary melatonin level in Japanese students
Background: Epidemiological studies in Japan have documented an association between morning type and a tryptophan-rich breakfast followed by exposure to sunlight in children. The association may be mediated by enhanced melatonin synthesis, which facilitates sleep at night. However, melatonin is inhibited by artificial light levels with high color-temperature common in Japanese homes at night. In this study, we investigated whether a combination of tryptophan-rich breakfast and light with low color-temperature at night could enhance melatonin secretion and encourage earlier sleep times Methods: The intervention included having breakfast with protein- and vitamin B6 - rich foods and exposure to sunlight after breakfast plus exposure to incandescent light (low temperature light) at night (October-November, 2010). The participants were 94 members of a university soccer club, who were divided into 3 groups for the intervention (G1: no intervention; G2: asked to have protein-rich foods such as fermented soybeans and vitamin B6-rich foods such as bananas at breakfast and sunlight exposure after breakfast; G3: the same contents as G2 and incandescent light exposure at night). Salivary melatonin was measured around 11:00 p.m. on the day before the beginning, a mid-point and on the day before the last day a mid-point and on the last day of the 1 month intervention. Results: In G3, there was a significantly positive correlation between total hours the participants spent under incandescent light at night and the frequency of feeling sleepy during the last week (p = 0.034). The salivary melatonin concentration of G3 was significantly higher than that of G1 and G2 in combined salivary samplings at the mid-point and on the day before the last day of the 1 month intervention (p = 0.018), whereas no such significant differences were shown on the day just before the start of the intervention (p = 0.63). Conclusion: The combined intervention on breakfast, morning sunlight and evening-lighting seems to be effective for students including athletes to keep higher melatonin secretion at night which seems to induce easy onset of the night sleep and higher quality of sleep
Structural basis for PPARγ transactivation by endocrine-disrupting organotin compounds
Harada, S., Hiromori, Y., Nakamura, S. et al. Structural basis for PPARγ transactivation by endocrine-disrupting organotin compounds. Sci Rep 5, 8520 (2015). https://doi.org/10.1038/srep08520
Combined use of bFGF and GDF-5 enhances the healing of medial collateral ligament injury
Basic fibroblast growth factor (bFGF) and growth and differentiation factor (GDF)-5 stimulate the healing of medial collateral ligament (MCL) injury. However, the effect of isolated and combined use of bFGF/GDF-5 remains still unclear. We investigated cellular proliferation and migration responding to bFGF/GDF-5 using rabbit MCL fibroblasts. Rabbit MCL injury was treated by bFGF and/or GDF-5 with peptide hydrogels. Gene expression and deposition of collagens in healing tissues were evaluated. bFGF/GDF-5 treatment additively enhanced cell proliferation and migration. bFGF/GDF-5 hydrogels stimulated Col1a1 expression without increasing Col3a1 expression. Combined use of bFGF/GDF-5 stimulated type I collagen deposition and the reorganization of fiber alignment, and induced better morphology of fibroblasts in healing MCLs. Our study indicates that combined use of bFGF/GDF-5 might enhance MCL healing by increasing proliferation and migration of MCL fibroblasts, and by regulating collagen synthesis and connective fiber alignment
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