Investigating xylem embolism formation, refilling and water storage in tree trunks using frequency domain reflectometry

Trunks of large trees play an important role in whole-plant water balance but technical difficulties have limited most hydraulic research to small stems, leaves, and roots. To investigate the dynamics of water-related processes in tree trunks, such as winter embolism refilling, xylem hydraulic vulnerability, and water storage, volumetric water content (VWC) in the main stem was monitored continuously using frequency domain moisture sensors in adult Betula papyrifera trees from early spring through the beginning of winter. An air injection technique was developed to estimate hydraulic vulnerability of the trunk xylem. Trunk VWC increased in early spring and again in autumn, concurrently with root pressure during both seasons. Diurnal fluctuations and a gradual decrease in trunk VWC through the growing season were observed, which, in combination with VWC increase after significant rainfall events and depletion during periods of high water demand, indicate the importance of stem water storage in both short- and long-term water balance. Comparisons between the trunk air injection results and conventional branch hydraulic vulnerability curves showed no evidence of ‘vulnerability segmentation’ between the main stem and small branches in B. papyrifera. Measurements of VWC following air injection, together with evidence from air injection and xylem dye perfusion, indicate that embolized vessels can be refilled by active root pressure but not in the absence of root pressure. The precise, continuous, and non-destructive measurement of wood water content using frequency domain sensors provides an ideal way to probe many hydraulic processes in large tree trunks that are otherwise difficult to investigate.Organismic and Evolutionary Biolog

Transfer function based input impedance determination of triple active bridge converter

The concept of multiport dc-dc converter was proposed to reduce the conversion stages of dc microgrid on more electric aircraft (MEA). The structure of multiport dc-dc converter is basically developed from the dual active bridge (DAB) converter because of its galvanic isolation and bidirectional power flow. A power electronics converter as a key element of the electrical power distribution system may cause stability issues. To address these challenges, the impedance characteristic of the multiport converter will be analyzed. In this paper, a transfer function based small signal model is developed and validated with a switching model, to figure out the characteristic of input impedance of triple active bridge (TAB) converter. Preliminary experimental results are presented to be as a support

A Fast Diagnosis Method for Both IGBT Faults and Current Sensor Faults in Grid-Tied Three-Phase Inverters With Two Current Sensors

© 1986-2012 IEEE. This article considers fault detection in the case of a three-phase three-wire (3P3W) inverter, when only two current sensors are used to save cost or due to a faulty current sensor. With two current sensors, there is no current method addressing the diagnosis of both IGBT open-circuit (OC) faults and current sensor faults. In order to solve this problem, this article proposes a method which innovatively combines two kinds of diagnosis variables, line voltage deviations and phase voltage deviations. The unique faulty characteristics of diagnosis variables for each fault are extracted and utilized to distinguish the fault. Using an average model, the method only needs the signals already available in the controller. Both IGBT OC faults and current sensor faults can be detected quickly in inverter mode and rectifier mode, so that the converter can be protected in a timely way to avoid further damages. In addition, error-adaptive thresholds are adopted to make the method robust. Effects such as system unbalance are analyzed to ensure that the method is robust and feasible. Simulation and experimental results are used to verify and validate the effectiveness of the method

A single-phase bidirectional AC/DC converter for V2G applications

This paper presents a single-phase bidirectional current-source AC/DC converter for vehicle to grid (V2G) applications. The presented converter consists of a line frequency commutated unfolding bridge and an interleaved buck-boost stage. The low semiconductor losses of the line frequency commutated unfolding bridge contribute to the comparatively good efficiency of this converter. The buck and boost operating modes of the interleaved buck-boost stage provide operation over a wide battery voltage range. The interleaved structure of the interleaved buck-boost stage results in lower battery current ripple. In addition, sinusoidal input current, bidirectional power flow and reactive power compensation capability are also guaranteed. This paper presents the topology and operating principles of the presented converter. The feasibility of the converter is validated using MATLAB simulations, as well as experimental results

Very long optical path-length from a compact multi-pass cell

The multiple-pass optical cell is an important tool for laser absorption spectroscopy and its many applications. For most practical applications, such as trace-gas detection, a compact and robust design is essential. Here we report an investigation into a multi-pass cell design based on a pair of cylindrical mirrors, with a particular focus on achieving very long optical paths. We demonstrate a path-length of 50.31 m in a cell with 40 mm diameter mirrors spaced 88.9 mm apart - a 3-fold increase over the previously reported longest path-length obtained with this type of cell configuration. We characterize the mechanical stability of the cell and describe the practical conditions necessary to achieve very long path-lengths

Clonal Dynamics In Vivo of Virus Integration Sites of T Cells Expressing a Safety Switch

Safety switches are becoming relevant for the clinical translation of T-cell-based immunotherapies. In patients receiving an allogeneic hematopoietic stem cell transplant, the inducible caspase-9 gene (iC9) safety switch expressed by donor-derived T lymphocytes efficiently controls acute graft versus host disease (GvHD). However, in vivo elimination of iC9-T cells by the chemical inducer of dimerization (CID) that activates the iC9 protein is incomplete. To study this effect, we characterized the clonal diversity and dynamics of vector insertion sites (VIS) in iC9-T cells pre- and post-CID administration in four patients who developed GvHD. We identified 3,203 VIS among four patients and followed their in vivo clonal dynamics up to 161 days post-CID. VIS were categorized by their proximity to host genome elements, gene associations, and cis-modulatory relationship to mapped promoters. We found that VIS are preferentially located near open chromatin and promoter regions; furthermore, there was no evidence for selection bias among VIS surviving the CID treatment. The majority of iC9-T cells with high normalized VIS copy number at the time of GvHD onset were eliminated by CID, while iC9-T cells detectable post-CID generally have low normalized VIS copy number. We propose that suboptimal iC9 transgene expression is responsible for the incomplete elimination of iC9-T cells and illustrate here by simple model how cis-modulatory influences of local genome context and T-cell receptor activation status at time of CID treatment contribute to stochastic sparing of iC9-T cells

Observations of SN 2017ein Reveal Shock Breakout Emission and A Massive Progenitor Star for a Type Ic Supernova

We present optical and ultraviolet observations of nearby type Ic supernova SN 2017ein as well as detailed analysis of its progenitor properties from both the early-time observations and the prediscovery Hubble Space Telescope (HST) images. The optical light curves started from within one day to $\sim$275 days after explosion, and optical spectra range from $\sim$2 days to $\sim$90 days after explosion. Compared to other normal SNe Ic like SN 2007gr and SN 2013ge, \mbox{SN 2017ein} seems to have more prominent C{\footnotesize II} absorption and higher expansion velocities in early phases, suggestive of relatively lower ejecta mass. The earliest photometry obtained for \mbox{SN 2017ein} show indications of shock cooling. The best-fit obtained by including a shock cooling component gives an estimate of the envelope mass as $\sim$0.02 M$_{\odot}$ and stellar radius as 8$\pm$4 R$_{\odot}$. Examining the pre-explosion images taken with the HST WFPC2, we find that the SN position coincides with a luminous and blue point-like source, with an extinction-corrected absolute magnitude of M$_V$$\sim$$-$8.2 mag and M$_I$$\sim$$-$7.7 mag.Comparisons of the observations to the theoretical models indicate that the counterpart source was either a single WR star or a binary with whose members had high initial masses, or a young compact star cluster. To further distinguish between different scenarios requires revisiting the site of the progenitor with HST after the SN fades away.Comment: 28 pages, 19 figures; accepted for publication in The Astrophysical Journa

Two methods for controlling three-time fundamental frequency neutral-point voltage oscillation in a hybrid VIENNA rectifier

This study presents two methods of controlling neutral-point voltage oscillation in a hybrid VIENNA rectifier, which is composed of the parallel association of a three-phase single-switch Boost rectifier with a VIENNA-type rectifier. The neutral-point oscillation reason has been analysed with a mathematical model. Meanwhile, the two neutral-point control methods of a simplified method based on a zero-sequence component injection and a dual-carrier pulse-width modulation (PWM) method are proposed to control the voltage deviation of the split DC-link and three-time fundamental frequency neutral-point voltage fluctuation with a decrease from ±1.6 to ±1 V, respectively. Moreover, the significant oscillation in the neutral-point voltage caused by unbalanced loads or asymmetric capacitor parameters can also be effectively suppressed by using the dual-carrier PWM method. Furthermore, the performance comparison between these two methods is provided. The experimental results show that the system after being introduced the proposed two methods still exhibits a low-order input current harmonic such as second, third, and fourth harmonics as well as the input current total harmonic distortion is lower than the standard 5%