Effect of Yushen zhuyun decoction on rats with diminished ovarian reserve induced by tripterygium glycosides

Purpose: To investigate the effect of Yushen zhuyun decoction (YSZYF) on rats with diminished ovarian reserve (DOR).Methods: High-performance liquid chromatography (HPLC) was used to determine the major phytochemical constituents of YSZYF. Rats with DOR (DOR rats) were prepared by administration of tripterygium glycosides (TWP) orally (50 mg/kg) for 15 days. Thereafter, DOR rats were treated orally with YSZYF (300, 600 and 900 mg/kg). After 15 days’ treatment, ovary index was calculated and blood was obtained to determine serum levels of follicle-stimulating hormone (FSH), estradiol (E2), progesterone (P), testosterone (T), inhibin (INH) and anti-mullerian hormone (AMH) by radioimmunoassay and enzyme-linked immunosorbent assay (ELISA). In addition, the ovary was subjected to histopathological examinations.Results: Phytochemical investigation indicated that the major constituents of YSZYF are acteoside, loganin, lcariin and echinacoside. Compared to the control rats, YSZYF treatment enhanced the ovary index of DOR rat (p < 0.05); furthermore, YSZYF treatment also enhanced the number of follicles and corpus luteum, as well as alleviated inflammatory reaction in ovary tissues. Additionally, the serum levels of FSH and T were elevated by treatment of YSZYF (p < 0.01), whereas E2, INHB and AMH concentrations decreased (p < 0.01), compared to control rats.Conclusion: The findings indicate that YSZYF improves ovarian reserve of DOR rats, and thus has a potential for treating infertility.Keywords: Yushen zhuyun decoction, Diminished ovarian reserve, Infertility, Acteoside, Loganin, Lcariin, Echinacosid

Innovative Engineering Education in Circuits and Systems

Nowadays, the field of microelectronics has become the drive for the advancement of the times, which promotes new demands on the cultivation of the students in colleges and universities. In order to keep up with the trend of the global engineering educational reform, three important reforms in education have been in progress step by step, including classroom teaching, innovative training and virtual laboratories. At first, for enhancing and integrating the existing courses related to the circuit, so that the students can comprehend the existing knowledge much more effectively, an important and effective curriculum reform has been performed by combining “Circuit Analysis” and “Analog Circuit Foundation” into one course; Then, innovative training has been carried out to cultivate the team skills among the students; Finally, in consideration of the rapid development of the electrical and electronic experiment, the conventional laboratory equipment may not satisfy the demand of every student due to financial constraints, therefore, the construction of virtual simulation experiment center is an efficient way to break this bottleneck. As a result, the atmosphere of academic innovation of the pursuit of truth, advocacy of science, brave exploration, dare to practice have been formed in colleges and universities through the above innovative engineering education reform

High resolution mapping and positional cloning of ENU-induced mutations in the Rw region of mouse chromosome 5

<p>Abstract</p> <p>Background</p> <p>Forward genetic screens in mice provide an unbiased means to identify genes and other functional genetic elements in the genome. Previously, a large scale ENU mutagenesis screen was conducted to query the functional content of a ~50 Mb region of the mouse genome on proximal Chr 5. The majority of phenotypic mutants recovered were embryonic lethals.</p> <p>Results</p> <p>We report the high resolution genetic mapping, complementation analyses, and positional cloning of mutations in the target region. The collection of identified alleles include several with known or presumed functions for which no mutant models have been reported (<it>Tbc1d14</it>, <it>Nol14</it>, <it>Tyms</it>, <it>Cad</it>, <it>Fbxl5</it>, <it>Haus3</it>), and mutations in genes we or others previously reported (<it>Tapt1</it>, <it>Rest</it>, <it>Ugdh</it>, <it>Paxip1</it>, <it>Hmx1, Otoe, Nsun7</it>). We also confirmed the causative nature of a homeotic mutation with a targeted allele, mapped a lethal mutation to a large gene desert, and localized a spermiogenesis mutation to a region in which no annotated genes have coding mutations. The mutation in <it>Tbc1d14 </it>provides the first implication of a critical developmental role for RAB-GAP-mediated protein transport in early embryogenesis.</p> <p>Conclusion</p> <p>This collection of alleles contributes to the goal of assigning biological functions to all known genes, as well as identifying novel functional elements that would be missed by reverse genetic approaches.</p

Ginger Stimulates Hematopoiesis via Bmp Pathway in Zebrafish

) has been widely used in traditional medicine; however, to date there is no scientific research documenting the potential of ginger to stimulate hematopoiesis. expression in the caudal hematopoietic tissue area. We further confirmed that Bmp/Smad pathway mediates this hematopoiesis promoting effect of ginger by using the Bmp-activated Bmp type I receptor kinase inhibitors dorsomorphin, LND193189 and DMH1.Our study provides a strong foundation to further evaluate the molecular mechanism of ginger and its bioactive components during hematopoiesis and to investigate their effects in adults. Our results will provide the basis for future research into the effect of ginger during mammalian hematopoiesis to develop novel erythropoiesis promoting agents

Multidifferential study of identified charged hadron distributions in ZZ-tagged jets in proton-proton collisions at s=\sqrt{s}=13 TeV

Jet fragmentation functions are measured for the first time in proton-proton collisions for charged pions, kaons, and protons within jets recoiling against a $Z$ boson. The charged-hadron distributions are studied longitudinally and transversely to the jet direction for jets with transverse momentum 20 $< p_{\textrm{T}} < 100$ GeV and in the pseudorapidity range $2.5 < \eta < 4$. The data sample was collected with the LHCb experiment at a center-of-mass energy of 13 TeV, corresponding to an integrated luminosity of 1.64 fb$^{-1}$. Triple differential distributions as a function of the hadron longitudinal momentum fraction, hadron transverse momentum, and jet transverse momentum are also measured for the first time. This helps constrain transverse-momentum-dependent fragmentation functions. Differences in the shapes and magnitudes of the measured distributions for the different hadron species provide insights into the hadronization process for jets predominantly initiated by light quarks.Comment: All figures and tables, along with machine-readable versions and any supplementary material and additional information, are available at https://cern.ch/lhcbproject/Publications/p/LHCb-PAPER-2022-013.html (LHCb public pages

Study of the B−→Λc+Λˉc−K−B^{-} \to \Lambda_{c}^{+} \bar{\Lambda}_{c}^{-} K^{-} decay

The decay $B^{-} \to \Lambda_{c}^{+} \bar{\Lambda}_{c}^{-} K^{-}$ is studied in proton-proton collisions at a center-of-mass energy of $\sqrt{s}=13$ TeV using data corresponding to an integrated luminosity of 5 $\mathrm{fb}^{-1}$ collected by the LHCb experiment. In the $\Lambda_{c}^+ K^{-}$ system, the $\Xi_{c}(2930)^{0}$ state observed at the BaBar and Belle experiments is resolved into two narrower states, $\Xi_{c}(2923)^{0}$ and $\Xi_{c}(2939)^{0}$, whose masses and widths are measured to be $$m(\Xi_{c}(2923)^{0}) = 2924.5 \pm 0.4 \pm 1.1 \,\mathrm{MeV}, \\ m(\Xi_{c}(2939)^{0}) = 2938.5 \pm 0.9 \pm 2.3 \,\mathrm{MeV}, \\ \Gamma(\Xi_{c}(2923)^{0}) = \phantom{000}4.8 \pm 0.9 \pm 1.5 \,\mathrm{MeV},\\ \Gamma(\Xi_{c}(2939)^{0}) = \phantom{00}11.0 \pm 1.9 \pm 7.5 \,\mathrm{MeV},$$ where the first uncertainties are statistical and the second systematic. The results are consistent with a previous LHCb measurement using a prompt $\Lambda_{c}^{+} K^{-}$ sample. Evidence of a new $\Xi_{c}(2880)^{0}$ state is found with a local significance of $3.8\,\sigma$, whose mass and width are measured to be $2881.8 \pm 3.1 \pm 8.5\,\mathrm{MeV}$ and $12.4 \pm 5.3 \pm 5.8 \,\mathrm{MeV}$, respectively. In addition, evidence of a new decay mode $\Xi_{c}(2790)^{0} \to \Lambda_{c}^{+} K^{-}$ is found with a significance of $3.7\,\sigma$. The relative branching fraction of $B^{-} \to \Lambda_{c}^{+} \bar{\Lambda}_{c}^{-} K^{-}$ with respect to the $B^{-} \to D^{+} D^{-} K^{-}$ decay is measured to be $2.36 \pm 0.11 \pm 0.22 \pm 0.25$, where the first uncertainty is statistical, the second systematic and the third originates from the branching fractions of charm hadron decays.Comment: All figures and tables, along with any supplementary material and additional information, are available at https://cern.ch/lhcbproject/Publications/p/LHCb-PAPER-2022-028.html (LHCb public pages

A Sequential Approach for Integrated Coal and Gas Mining of Closely-Spaced Outburst Coal Seams: Results from a Case Study Including Mine Safety Improvements and Greenhouse Gas Reductions

Closely-spaced outburst coal seams (COCS) is the main condition of coal seams in southwest China, and gas disasters are one of the major problems affecting coal mine safety. Mining a protective seam and pre gas extraction are the most safety way to improve the efficiency of mining under these conditions. However, low pre-mining gas extraction efficiency coupled with the close proximity of adjacent working faces is a problem. When mining at an old working face has been completed but the new working face is not yet ready to be mined, coal-bed gasses can flow into the new working face from adjacent seams and this commonly causes methane monitoring instruments to sound an alarm. These gas extraction difficulties lead to a conflict between mine safety and profit. To solve these problems, a sequential approach for integrated coal and gas mining of closely-spaced outburst coal seams is introduced in this paper. Two fundamental principles are proposed: (1) Fully coordinating the spatiotemporal relationships between gas extraction, roadway development, and coal mining to maximize both mine safety and coal and gas production; (2) Defining a mining sequence for outburst coal seams and choosing the coal seam with the weakest outburst risk as the protective seam. A system for comprehensive gas extraction in underground coal mines is divided into four stages for gas extraction: gas extraction before coal roadway tunneling, gas extraction before coal mining, gas extraction during coal mining, and gas extraction from the goaf after coal mining. The Songzao mining area, China, is used as a case study to demonstrate the effectiveness of this model, and it brings three major benefits: it improves underground coal mine safety with physical gas accidents decreased by 66.8%, it makes underground coal-bed methane (CBM) extraction more efficient with the average gas extraction rate were respectively 45.13 m3/t and 62.4%, the highest in China, and it reduces greenhouse gas emissions equivalent to 3.5 million tonnes of carbon dioxide. This study could be used as a valuable example for other coal deposits being mined under similar geological conditions

Research on the High Sensitivity Detection Method of Carbon Nanotube/Polydimethylsiloxane Composites Structure

In this paper, a carbon nanotube (CNT)/polydimethylsiloxane (PDMS) composite force-sensitive structure with good flexibility is proposed and fabricated, and the measurement of scanning electron microscopy (SEM) and Raman are carried out. The equivalent circuit of force-sensitive test of structure is performed and analyzed under direct current (DC) and alternating current (AC) conditions. Under AC conditions, experimental results further show that the sensitivity and sensitivity factors of force-sensitive structures are 0.15 KPa−1 and 2.17 in the pressure range of 600–1000 KPa compressive stress and 20–50% tensile stress, respectively. These results are increased by 36.4% and 38.2% compared to the results of compressive stress (0.11 KPa−1) and tensile stress (1.57) under DC conditions, respectively. It shows that the carbon nanotube/PDMS composite has higher test accuracy under AC conditions

Isolated Fe-Co heteronuclear diatomic sites as efficient bifunctional catalysts for high-performance lithium-sulfur batteries

The slow redox kinetics of polysulfides and the difficulties in decomposition of Li2S are two serious obstacles to lithium-sulfur batteries. Here, the authors report an isolated Fe-Co heteronuclear diatomic catalyst to achieve high efficiency bifunctional catalysis for lithium-sulfur batteries

Plasmon–Microcavity Coupling and Fabry–Pèrot Lasing in a ZnO:Ga Microwire/p-Type Gallium Nitride Heterojunction

In recent years, electroluminescent devices of ZnO have been the focus of attention and research. In this paper, we fabricated a ZnO:Ga microwire/p-type gallium nitride heterojunction light-emitting diode and found a lasing emission near the silver electrode. We interpret this lasing as trap-state FP-mode lasing because a series of small peaks appear in the spectrum and the positions of three lasing peaks are close to the emission peak from the trap state. After sputtering gold on the surface of the ZnO:Ga microwire, the luminescence of the device was enhanced and anomalous spectral signals appeared at a reverse current of 20 mA. The luminescence enhancement is due to the hot electron transfer induced by plasmons, and the strange spectral phenomenon was attributed to the Fano resonance caused by plasmon–microcavity coupling. The above research can provide some guidance for the design of LEDs and laser devices