Auswirkungen adrenerger Stimulation auf tumorspezifische Signalsysteme bei Zellen mit induzierter Expression von P-Cadherin

Der Einfluss adrenerger Stimulation auf Zellen des oralen Plattenepithelkarzinoms wurde untersucht, indem Zellen mit induzierter Expression von P-Cadherin anderen Zellen vergleichend gegenüber gestellt wurden, die dieses Protein nicht exprimieren. Untersuchungen wurden proteinbiochemisch mittels Western Blot hinsichtlich der Expression von PD-L1, Beta-Catenin, GSK-3beta, alpha- und beta-adrenergen Rezeptoren durchgeführt. Zudem wurden funktionelle Versuche zu vaskulogenen Mimikry, Migration, Invasion und Proliferation durchgeführt. Es zeigten sich bei der Expression der untersuchten Proteine keine signifikaten Unterschiede, die auf eine adrenerge Stimulation in Zusammenhang mit einer etwaigen Expression von P-Cadherin zurückzuführen wäre. In den funktionellen Versuchen konnte für die Invasion unter Expression von P-Cadherin ein signifikanter Rückgang der Invasion in vitro durch beta-adrenerge Stimulation nachgewiesen werden. Ansonsten blieben die funktionellen Assays ohne signifikante Ergebnisse

Alloplastic Reconstruction of the Extensor Mechanism after Resection of Tibial Sarcoma

Reconstruction of the extensor mechanism is essential for good extremity function after endoprosthetic knee replacement following tumor resection. Only a few biological methods have been able to reliably restore a functional extensor mechanism, but they are often associated with significant complication rates. Reattachment of the patellar tendon to the prosthesis using an alloplastic patellar ligament (Trevira cord) can be an appropriate alternative. In vivo and in vitro studies have already shown that complete fibrous ingrowth in polyethylene chords can be seen after a period of six months. However, until now, no biomechanical study has shown the efficacy of an alloplastic cord and its fixation device in providing sufficient stability and endurance in daily life-activity until newly formed scar tissue can take over this function. In a special test bench developed for this study, different loading regimes were applied to simulate loads during everyday life. Failure loads and failure modes were evaluated. The properties of the cord were compared before and after physiological conditioning. It was shown that rubbing was the mode of failure under dynamic loading. Tensile forces up to 2558 N did not result in material failure. Thus, using an artificial cord together with this fixation device, temporary sufficient stable fixation can be expected

Patients with Intolerance Reactions to Total Knee Replacement: Combined Assessment of Allergy Diagnostics, Periprosthetic Histology, and Peri-implant Cytokine Expression Pattern

We performed a combined approach to identify suspected allergy to knee arthroplasty (TKR): patch test (PT), lymphocyte transformation test (LTT), histopathology (overall grading; T- and B-lymphocytes, macrophages, and neutrophils), and semiquantitative Real-time-PCR-based periprosthetic inflammatory mediator analysis (IFN gamma, TNF alpha, IL1-beta, IL-2, IL-6, IL-8, IL-10, IL17, and TGF beta). We analyzed 25 TKR patients with yet unexplained complications like pain, effusion, and reduced range of motion. They consisted of 20 patients with proven metal sensitization (11 with PT reactions;9 with only LTT reactivity). Control specimens were from 5 complicated TKR patients without metal sensitization, 12 OA patients before arthroplasty, and 8 PT patients without arthroplasty. Lymphocytic infiltrates were seen and fibrotic (Type IV membrane) tissue response was most frequent in the metal sensitive patients, for example, in 81% of the PT positive patients. The latter also had marked periprosthetic IFN gamma expression. 8/9 patients with revision surgery using Ti-coated/oxinium based implants reported symptom relief. Our findings demonstrate that combining allergy diagnostics with histopathology and periprosthetic cytokine assessment could allow us to design better diagnostic strategies

Spectroscopija 125Te (n,γ), (d,p) i (3He,α) reakcijama

Single γ-ray spectra and γγ-coincidences, following thermal neutron capture in 124Te, were measured with semiconductor detectors at the light-water reactor LWR - 15 at Řež. Intensities of g transitions in 125Te were normalized using the absolute intensity of 7.8% of the 6620 keV line in 36Cl. The high resolution (d,p) measurements were performed with 17 MeV deuterons, using the Q3D spectrograph at two scattering angles of 15° and 30°. Spectra were recorded in the range up to 3.3 MeV and calibrated using the proton peaks with l =1, and the corresponding level energies were determined in the thermal neutron capture reaction. The (3He,α) experiment was carried out with a 32 MeV He beam at the angle of 10°. The spectrum was recorded in one run by means of a large detector in a range up to approximately 4.7 MeV. The absolute intensities were determined by measuring the total beam current.Mjerili su se izravni γ- i sudesni γ-γ-spektri nakon uhvata termičkih neutrona u 124Te. Mjerenja (d,p) reakcija visokog razlučivanja na toj jezgri izvela su se s deuteronima 17 MeV na kutovima od 15° i 30° , pomoću Q3D spektrografa. Također se mjerio spektar u reakciji (3He,α) na 10° . Na osnovi tih mjerenja utvrđena su mnoga nova stanja 125Te na energijama uzbude do oko 4.5 MeV. Razlika grananja 403/443 keV iz izravnih i sudesnih γ-spektara ukazuje na jaku primjesu E2 u primarnom prijelazu od 6125 keV

Spectroscopija 125Te (n,γ), (d,p) i (3He,α) reakcijama

Single γ-ray spectra and γγ-coincidences, following thermal neutron capture in 124Te, were measured with semiconductor detectors at the light-water reactor LWR - 15 at Řež. Intensities of g transitions in 125Te were normalized using the absolute intensity of 7.8% of the 6620 keV line in 36Cl. The high resolution (d,p) measurements were performed with 17 MeV deuterons, using the Q3D spectrograph at two scattering angles of 15° and 30°. Spectra were recorded in the range up to 3.3 MeV and calibrated using the proton peaks with l =1, and the corresponding level energies were determined in the thermal neutron capture reaction. The (3He,α) experiment was carried out with a 32 MeV He beam at the angle of 10°. The spectrum was recorded in one run by means of a large detector in a range up to approximately 4.7 MeV. The absolute intensities were determined by measuring the total beam current.Mjerili su se izravni γ- i sudesni γ-γ-spektri nakon uhvata termičkih neutrona u 124Te. Mjerenja (d,p) reakcija visokog razlučivanja na toj jezgri izvela su se s deuteronima 17 MeV na kutovima od 15° i 30° , pomoću Q3D spektrografa. Također se mjerio spektar u reakciji (3He,α) na 10° . Na osnovi tih mjerenja utvrđena su mnoga nova stanja 125Te na energijama uzbude do oko 4.5 MeV. Razlika grananja 403/443 keV iz izravnih i sudesnih γ-spektara ukazuje na jaku primjesu E2 u primarnom prijelazu od 6125 keV

Extrakorporale hydrostatische Hochdruckbehandlung als neues Verfahren zur Desinfektion infizierter Knochenpräparate

Background: Allogeneic bone transplantation is at risk of infection, and established disinfection methods typically compromise bone quality. High hydrostatic pressure (HHP) is well established for disinfection in food technology, and also it does protect biomechanical and biological properties of bone. This study is the first investigation of HHP regarding disinfection of bone biopsies. Materials and methods: Bone biopsies of 34 patients with chronic infections were subjected to HHP and assessed for persisting bacterial growth. In series 1, bone biopsies were proceeded directly to HHP (10 min; maximal pressure P-max 600 MPa). In series 2, HHP was applied after 5-day incubation in growth media (10 min or 2 x 30 min; P-max 600 MPa). Furthermore, HHP-induced changes of bacterial morphology on artificially infected bone samples were evaluated by scanning electron microscopy (SEM). Results: For series 1, 71% of the bone samples were sterilised by HHP (n = 17), compared to 38% of the untreated control samples, which were obtained during the same surgery (n = 8). For series 2, after prior incubation, HHP disinfected 7% of the bone specimens (n = 55), all control samples showed bacterial growth (n = 33). Destruction of cell wall integrity of Gram-negative strains was observed by SEM. Conclusion: The effectiveness of HHP for bone disinfection should be improved by optimising treatment parameters. Infections with barosensitive Gram-negative bacteria or yeast might represent possible clinical indications

Clinically Relevant Effectiveness of Focused Extracorporeal Shock Wave Therapy in the Treatment of Chronic Plantar Fasciitis A Randomized, Controlled Multicenter Study

Background: The effectiveness of extracorporeal shock wave therapy in the treatment of plantar fasciitis is controver