Nitric oxide: Its generation and interactions with other reactive signaling compounds

Nitric oxide (NO) is an immensely important signaling molecule in animals and plants. It is involved in plant reproduction, development, key physiological responses such as stomatal closure, and cell death. One of the controversies of NO metabolism in plants is the identification of enzymatic sources. Although there is little doubt that nitrate reductase (NR) is involved, the identification of a nitric oxide synthase (NOS)-like enzyme remains elusive and it is becoming increasingly clear that such a protein does not exist in higher plants, even though homologues have been found in algae. Downstream from its production, NO can have several potential actions, but none of these will be in isolation from other reactive signaling molecules which have similar chemistry to NO. Therefore, NO metabolism will be taking place in an environment containing reactive oxygen species (ROS), hydrogen sulfide (H2S), glutathione, other antioxidants and within a reducing redox state. Direct reactions with NO are likely to produce new signaling molecules such as peroxynitrite and nitrosothiols, and it is probable that chemical competitions will exist which will determine the ultimate end result of signaling responses. How NO is generated in plants cells and how NO fits into this complex cellular environment needs to be understood

Improved Sensitivity for Space Domain Awareness Observations with the Murchison Widefield Array

Our previously reported survey of the Low Earth Orbit (LEO) environment using the Murchison Widefield Array (MWA) detected over 70 unique Resident Space Objects (RSOs) over multiple passes, from 20 hours of observations in passive radar mode. In this paper, we extend this work by demonstrating two methods that improve the detection sensitivity of the system. The first method, called shift-stacking, increases the statistical significance of faint RSO signals through the spatially coherent integration of the reflected signal along the RSO's trajectory across the sky. This method was tested on the observations used during our previous blind survey, and we obtained a $75\%$ increase in the total number of detections. The second method re-focuses the MWA to the near-field RSO's position (post-observation), by applying a complex phase correction to each visibility to account for the curved wave-front. The method was tested successfully on an MWA extended array observation of an ISS pass. However, the method is currently limited by signal de-coherence on the long-baselines (due to the hardware constraints of the current correlator). We discuss the sensitivity improvement for RSO detections we expect from the MWA Phase 3 correlator upgrade. We conclude the paper by briefly commenting on future dedicated Space Domain Awareness (SDA) systems that will incorporate MWA technologies.Comment: Accepted in Advances in Space Research. 16 pages, 10 figures, and 1 Tabl

e-VLBI observations of GHz-Peaked Spectrum (GPS) radio sources in nearby galaxies from the AT20G survey

GHz-peaked spectrum (GPS) radio sources are thought to be young objects which later evolve into FR-I and FR-II radio galaxies. We have used the Australia Telescope 20GHz (AT20G) survey catalogue to select a uniform sample of GPS sources with spectral peaks above 5GHz, which should represent the youngest members of this class. In this paper, we present e-VLBI observations of ten such objects which are associated with nearby (z<0.15) galaxies and so represent a new population of local, low--power GPS sources. Our e-VLBI observations were carried out at 4.8GHz with the Australia Telescope Long Baseline Array (LBA) using a real--time software correlator. All ten sources were detected, and were unresolved on scales of ~100mas, implying that they are typically less than 100pc in linear size.Comment: 7 pages, 7 figures, 3 table

Improving models of urban greenspace: from vegetation surface cover to volumetric survey, using waveform laser scanning

This is the author accepted manuscript. The final version is available from Wiley via the DOI in this record.1 Urban greenspace has a major impact on human health and quality of life, and thus the way in which such green infrastructure is constructed, managed and maintained is of critical importance. A range of studies have demonstrated the relationship between the areal coverage and distribution of vegetation and the provision of multiple urban ecosystem services. It is not known how sensitive findings are to the spatial resolution of the underlying data relative to the grain size of urban land cover heterogeneity. Moreover, little is known about the three-dimensional (3D) structure of urban vegetation and delivery of services, and addressing such questions is limited by the availability of data describing canopy structure from the tree tops to the ground. 2 Waveform airborne laser scanning (lidar) offers a new way of capturing 3D data describing vegetation structure. We generated voxels (volumetric pixels) from waveform lidar (1.5 m resolution), differentiated vegetation layers using height as a determinant, and computed statistics on surface cover, volume and volume density per stratum. We then used a range of widely available remote sensing products with varying spatial resolution (1m to 100m) to map the same greenspace, and compared results to those from the waveform lidar survey. 3 We focused on data from three urban zones in the UK with distinct patterns of vegetation cover. We found -3%, +7.5% and +26.1% differences in green surface cover compared with, respectively, town planning maps (< 10 m resolution), national land cover maps (25 m) and European land cover maps (100 m). There were differences of -59.1%, +12.4% and -2.4% in tree cover compared with global (30 m resolution), European (25 m) and national (1 m) estimates. Waveform lidar captured sub-canopy structure and detected empty spaces in the understorey which contributed a 16% bias in the total green volume derived from non-waveform lidar observations. 4 We conclude that waveform lidar has a key role to play in estimating important quantitative metrics of urban green infrastructure, which is important because urban greenspace is highly fragmented and shows high levels of spatial and volumetric heterogeneity

Is waveform worth it? A comparison of LiDAR approaches for vegetation and landscape characterization

AcceptedArticleThis article has been accepted for publication in Remote Sensing in Ecology and Conservation, an Open Access journal in which articles are published under the terms of the Creative Commons Attribution Licence. The definitive published version will be made available via: http://onlinelibrary.wiley.com/journal/10.1002/(ISSN)2056-3485 © Zoological Society of LondonLight Detection and Ranging (LiDAR) systems are frequently used in ecological studies to measure vegetation canopy structure. Waveform LiDAR systems offer new capabilities for vegetation modelling by measuring the time-varying signal of the laser pulse as it illuminates different elements of the canopy, providing an opportunity to describe the 3D structure of vegetation canopies more fully. This article provides a comparison between waveform airborne laser scanning (ALS) data and discrete return ALS data, using terrestrial laser scanning (TLS) data as an independent validation. With reference to two urban landscape typologies, we demonstrate that discrete return ALS data provided more biased and less consistent measurements of woodland canopy height (in a 100% tree covered plot, height underestimation bias = 0.82 m; SD = 1.78 m) than waveform ALS data (height overestimation bias = 0.65 m; SD = 1.45 m). The same biases were found in suburban data (in a plot consisting of 100% hard targetse.g. roads and pavements), but discrete return ALS were more consistent here than waveform data (SD = 0.57 m compared to waveform SD = 0.76 m). Discrete return ALS data performed poorly in describing the canopy understorey, compared to waveform data. Our results also highlighted errors in discrete return ALS intensity, which were not present with waveform data. Waveform ALS data therefore offer an improved method for measuring the three-dimensional structure of vegetation systems, but carry a higher data processing cost. New toolkits for analysing waveform data will expedite future analysis and allow ecologists to exploit the information content of waveform LiDAR.Natural Environment Research Council (NERC

Ego depletion in visual perception: Ego-depleted viewers experience less ambiguous figure reversal.

This study examined the effects of ego depletion on ambiguous figure perception. Adults (N = 315) received an ego depletion task and were subsequently tested on their inhibitory control abilities that were indexed by the Stroop task (Experiment 1) and their ability to perceive both interpretations of ambiguous figures that was indexed by reversal (Experiment 2). Ego depletion had a very small effect on reducing inhibitory control (Cohen's d = .15) (Experiment 1). Ego-depleted participants had a tendency to take longer to respond in Stroop trials. In Experiment 2, ego depletion had small to medium effects on the experience of reversal. Ego-depleted viewers tended to take longer to reverse ambiguous figures (duration to first reversal) when naïve of the ambiguity and experienced less reversal both when naïve and informed of the ambiguity. Together, findings suggest that ego depletion has small effects on inhibitory control and small to medium effects on bottom-up and top-down perceptual processes. The depletion of cognitive resources can reduce our visual perceptual experience

The Human Cytomegalovirus Chemokine vCXCL-1 Modulates Normal Dissemination Kinetics of Murine Cytomegalovirus In Vivo

Human cytomegalovirus (HCMV) is a betaherpesvirus that is a significant pathogen within newborn and immunocompromised populations. Morbidity associated with HCMV infection is the consequence of viral dissemination. HCMV has evolved to manipulate the host immune system to enhance viral dissemination and ensure long-term survival within the host. The immunomodulatory protein vCXCL-1, a viral chemokine functioning primarily through the CXCR2 chemokine receptor, is hypothesized to attract CXCR2+ neutrophils to infection sites, aiding viral dissemination. Neutrophils harbor HCMV in vivo; however, the interaction between vCXCL-1 and the neutrophil has not been evaluated in vivo. Using the mouse model and mouse cytomegalovirus (MCMV) infection, we show that murine neutrophils harbor and transfer infectious MCMV and that virus replication initiates within this cell type. Utilizing recombinant MCMVs expressing vCXCL-1 from the HCMV strain (Toledo), we demonstrated that vCXCL-1 significantly enhances MCMV dissemination kinetics. Through cellular depletion experiments, we observe that neutrophils impact dissemination but that overall dissemination is largely neutrophil independent. This work adds neutrophils to the list of innate cells (i.e., dendritic and macrophages/monocytes) that contribute to MCMV dissemination but refutes the hypothesis that neutrophils are the primary cell responding to vCXCL-1

Identification of IncA/C plasmid replication and maintenance genes and development of a plasmid multilocus sequence typing scheme

Plasmids of incompatibility group A/C (IncA/C) are becoming increasingly prevalent within pathogenic Enterobacteriaceae. They are associated with the dissemination of multiple clinically relevant resistance genes, including blaCMY and blaNDM. Current typing methods for IncA/C plasmids offer limited resolution. In this study, we present the complete sequence of a blaNDM-1-positive IncA/C plasmid, pMS6198A, isolated from a multidrug-resistant uropathogenic Escherichia coli strain. Hypersaturated transposon mutagenesis, coupled with transposon-directed insertion site sequencing (TraDIS), was employed to identify conserved genetic elements required for replication and maintenance of pMS6198A. Our analysis of TraDIS data identified roles for the replicon, including repA, a toxin-antitoxin system; two putative partitioning genes, parAB; and a putative gene, 053. Construction of mini-IncA/C plasmids and examination of their stability within E. coli confirmed that the region encompassing 053 contributes to the stable maintenance of IncA/C plasmids. Subsequently, the four major maintenance genes (repA, parAB, and 053) were used to construct a new plasmid multilocus sequence typing (PMLST) scheme for IncA/C plasmids. Application of this scheme to a database of 82 IncA/C plasmids identified 11 unique sequence types (STs), with two dominant STs. The majority of blaNDM-positive plasmids examined (15/17; 88%) fall into ST1, suggesting acquisition and subsequent expansion of this blaNDM-containing plasmid lineage. The IncA/C PMLST scheme represents a standardized tool to identify, track, and analyze the dissemination of important IncA/C plasmid lineages, particularly in the context of epidemiological studies