PRODUCER ACCEPTANCE OF A NEW PEANUT MARKETING COOPERATIVE: A SURVEY OF GEORGIA PEANUT PRODUCERS

Market conduct has become an important issue for peanut farmers. Consolidation in the first buyer market, increased imports, and political uncertainty have increased peanut producers' marketing risks. The purpose of this paper was to examine demographic differences in peanut producers' perceptions of the current marketing environment as well as their attitudes towards new marketing institutions. A standard t-test revealed that producers growing more than 250 acres of peanuts, irrigating at least 50 percent of their peanuts, and producers located in Southwest Georgia were statistically more dissatisfied with the current marketing environment and significantly more receptive to forming a new generation peanut cooperative.Agribusiness, Crop Production/Industries,

A NEW GENERATION PEANUT COOPERATIVE IN GEORGIA: A BENEFIT-COST ANALYSIS

A survey of Georgia peanut producers revealed that the Southwest corner of Georgia could be targeted for a new generation peanut cooperative (FS 01-07). The objective of this study was to examine the feasibility of this option. Preliminary results revealed that this might be an economically feasible solution to peanut producers' marketing problems. The projected discounted benefit-cost ratios ranged from 1.9 to 1.4 over a ten-year period.Agribusiness, Crop Production/Industries,

Cavity ringdown studies of the E-H transition in an inductively coupled oxygen plasma: comparison of spectroscopic measurements and modelling

The absolute number density of ground state oxygen atoms, O(3P), present in a 100 mTorr oxygen plasma has been determined as a function of operating power using cavity ringdown spectroscopy (CRDS). The dissociation fraction increases by an order of magnitude from ∼0.8% at 50 W to 8% at 250 W and reflects a similar increase in the electron density over this power range. Emission spectra show that the E–H switchover is accompanied by increased rotational heating of O2 and this behaviour is also observed in the translational temperatures determined by fitting the Doppler limited O(3P) CRDS data. The measurements are contextualised via a volume averaged kinetic model that uses the measured absolute densities of O(3P) and O2(a1Δg, v = 0) as a function of power as its benchmarks. Despite the inherent spatial inhomogeneity of the plasma, the volume averaged model, which uses a minimal set of reactions, is able to both reproduce previous measurements on the absolute density of O− and to infer physically reasonable values for both the electron temperature and number density as the E–H switch over is traversed. Time-resolved emission measurements return a value of 0.2 for the wall loss coefficient for O2(b1Σg+); as a consequence, the number density of O2(b1Σg+) is (at least) one order of magnitude less than O2(a1Δg)

Targeting the Pseudomonas aeruginosa Virulence Factor Phospholipase C With Engineered Liposomes.

Engineered liposomes composed of the naturally occurring lipids sphingomyelin (Sm) and cholesterol (Ch) have been demonstrated to efficiently neutralize toxins secreted by Gram-positive bacteria such as Streptococcus pneumoniae and Staphylococcus aureus. Here, we hypothesized that liposomes are capable of neutralizing cytolytic virulence factors secreted by the Gram-negative pathogen Pseudomonas aeruginosa. We used the highly virulent cystic fibrosis P. aeruginosa Liverpool Epidemic Strain LESB58 and showed that sphingomyelin (Sm) and a combination of sphingomyelin with cholesterol (Ch:Sm; 66 mol/% Ch and 34 mol/% Sm) liposomes reduced lysis of human bronchial and red blood cells upon challenge with the Pseudomonas secretome. Mass spectrometry of liposome-sequestered Pseudomonas proteins identified the virulence-promoting hemolytic phospholipase C (PlcH) as having been neutralized. Pseudomonas aeruginosa supernatants incubated with liposomes demonstrated reduced PlcH activity as assessed by the p-nitrophenylphosphorylcholine (NPPC) assay. Testing the in vivo efficacy of the liposomes in a murine cutaneous abscess model revealed that Sm and Ch:Sm, as single dose treatments, attenuated abscesses by >30%, demonstrating a similar effect to that of a mutant lacking plcH in this infection model. Thus, sphingomyelin-containing liposome therapy offers an interesting approach to treat and reduce virulence of complex infections caused by P. aeruginosa and potentially other Gram-negative pathogens expressing PlcH

Activated mutant NRasQ61K drives aberrant melanocyte signaling, survival, and invasiveness via a rac1-Dependent mechanism

Around a fifth of melanomas exhibit an activating mutation in the oncogene NRas that confers constitutive signaling to proliferation and promotes tumor initiation. NRas signals downstream of the major melanocyte tyrosine kinase receptor c-kit and activated NRas results in increased signaling via the extracellular signal–regulated kinase (ERK)/MAPK/ERK kinase/mitogen-activated protein kinase (MAPK) pathways to enhance proliferation. The Ras oncogene also activates signaling via the related Rho GTPase Rac1, which can mediate growth, survival, and motility signaling. We tested the effects of activated NRasQ61K on the proliferation, motility, and invasiveness of melanoblasts and melanocytes in the developing mouse and ex vivo explant culture as well as in a melanoma transplant model. We find an important role for Rac1 downstream of NRasQ61K in mediating dermal melanocyte survival in vivo in mouse, but surprisingly NRasQ61K does not appear to affect melanoblast motility or proliferation during mouse embryogenesis. We also show that genetic deletion or pharmacological inhibition of Rac1 in NRasQ61K induced melanoma suppresses tumor growth, lymph node spread, and tumor cell invasiveness, suggesting a potential value for Rac1 as a therapeutic target for activated NRas-driven tumor growth and invasiveness

Independent discovery of a nulling pulsar with unusual subpulse drifting properties with the Murchison Widefield Array

We report the independent discovery of PSR J0026-1955 with the Murchison Widefield Array (MWA) in the ongoing Southern-sky MWA Rapid Two-metre pulsar survey. J0026-1955 has a period of ∼1.306 s, a dispersion measure of ∼20.869 pc cm-3, and a nulling fraction of ∼77%. This pulsar highlights the advantages of the survey's long dwell times (∼80 minutes), which, when fully searched, will be sensitive to the expected population of similarly bright, intermittent pulsars with long nulls. A single-pulse analysis in the MWA's 140-170 MHz band also reveals a complex subpulse drifting behavior, including both rapid changes of the drift rate characteristic of mode switching pulsars, as well as a slow, consistent evolution of the drift rate within modes. In some longer drift sequences, interruptions in the otherwise smooth drift rate evolution occur preferentially at a particular phase, typically lasting a few pulses. These properties make this pulsar an ideal test bed for prevailing models of drifting behavior such as the carousel model

Observing binary inspiral in gravitational radiation: One interferometer

We investigate the sensitivity of individual LIGO/VIRGO-like interferometers and the precision with which they can determine the characteristics of an inspiralling binary system. Since the two interferometers of the LIGO detector share nearly the same orientation, their joint sensitivity is similar to that of a single, more sensitive interferometer. We express our results for a single interferometer of both initial and advanced LIGO design, and also for the LIGO detector in the limit that its two interferometers share exactly the same orientation. We approximate the evolution of a binary system as driven exclusively by leading order quadrupole gravitational radiation. To assess the sensitivity, we calculate the rate at which sources are expected to be observed, the range to which they are observable, and the precision with which characteristic quantities describing the observed binary system can be determined. Assuming a conservative rate density for coalescing neutron star binary systems we expect that the advanced LIGO detector will observe approximately 69~yr${}^{-1}$ with an amplitude SNR greater than 8. Of these, approximately 7~yr${}^{-1}$ will be from binaries at distances greater than 950~Mpc. We explore the sensitivity of these results to a tunable parameter in the interferometer design (the recycling frequency). The optimum choice of the parameter is dependent on the goal of the observations, e.g., maximizing the rate of detections or maximizing the precision of measurement. We determine the optimum parameter values for these two cases.Comment: 40 pages (plus 7 figures), LaTeX/REVTEX3.0, NU-GR-

Cyclic ADP ribose isomers: Production, chemical structures, and immune signaling

Cyclic adenosine diphosphate (ADP)–ribose (cADPR) isomers are signaling molecules produced by bacterial and plant Toll/interleukin-1 receptor (TIR) domains via nicotinamide adenine dinucleotide (oxidized form) (NAD+) hydrolysis. We show that v-cADPR (2′cADPR) and v2-cADPR (3′cADPR) isomers are cyclized by O-glycosidic bond formation between the ribose moieties in ADPR. Structures of 2′cADPR-producing TIR domains reveal conformational changes that lead to an active assembly that resembles those of Toll-like receptor adaptor TIR domains. Mutagenesis reveals a conserved tryptophan that is essential for cyclization. We show that 3′cADPR is an activator of ThsA effector proteins from the bacterial antiphage defense system termed Thoeris and a suppressor of plant immunity when produced by the effector HopAM1. Collectively, our results reveal the molecular basis of cADPR isomer production and establish 3′cADPR in bacteria as an antiviral and plant immunity–suppressing signaling molecule

Current experiences and educational preferences of general practitioners and staff caring for people with dementia living in residential facilities

<p>Abstract</p> <p>Background</p> <p>Residential care is important for older adults, particularly for those with advanced dementia and their families. Education interventions that achieve sustainable improvement in the care of older adults are critical to quality care. There are few systematic data available regarding the educational needs of Residential Care Facility (RCF) staff and General Practitioners (GPs) relating to dementia, or the sustainability of educational interventions. We sought to determine participation in dementia education, perceived levels of current knowledge regarding dementia, perceived unmet educational needs, current barriers, facilitators and preferences for dementia education.</p> <p>Methods</p> <p>A mixed methods study design was utilised. A survey was distributed to a convenience sample of general practitioners, and staff in 223 consecutive residential care facilities in Perth, Western Australia. Responses were received from 102 RCF staff working in 10 facilities (out of 33 facilities who agreed to distribute the survey) and 202 GPs (19% of metropolitan GPs). Quantitative survey data were summarised descriptively and chi squared statistics were used to analyse the distribution of categorical variables. Qualitative data were collected from general practitioners, staff in residential care facilities and family carers of people with dementia utilizing individual interviews, surveys and focus groups. Qualitative data were analysed thematically.</p> <p>Results</p> <p>Among RCF staff and GPs attending RCF, participation in dementia education was high, and knowledge levels generally perceived as good. The individual experiences and needs of people with dementia and their families were emphasised. Participants identified the need for a person centred philosophy to underpin educational interventions. Limited time was a frequently mentioned barrier, especially in relation to attending dementia care education. Perceived educational needs relating to behaviours of concern, communication, knowledge regarding dementia, aspects of person centred care, system factors and the multidisciplinary team were consistently and frequently cited. Small group education which is flexible, individualized, practical and case based was sought.</p> <p>Conclusion</p> <p>The effectiveness and sustainability of an educational intervention based on these findings needs to be tested. In addition, future interventions should focus on supporting cultural change to facilitate sustainable improvements in care.</p