Model Hubungan Antara Volume Lalulintas Dengan Tarif Jalan Tol

Indonesia has experienced increasing economic growth every year. This recent trend needs to be supported by adequate transportation infrastructures, especially roads. Since there is limited budget for infrastructure development, the government has invited private investors for toll road construction. Toll tariff and traffic volume are two main factors that affect toll road income and investment. A method based on financial approach needs to be developed to enhance the benefit cost analysis of toll road construction and furthermore to determine the toll tariff. Factors that affect toll tariff were analyzed based on vehicle number and vehicle growth rate. The elasticity theory was applied in this case study to identify the effects of toll tariff on traffic volume. A model of critical traffic volume was created based on the analysis of several factors such as construction cost, operation and maintenance cost, payback period, and internal rate of return. The results from Jia method and the Present Worth Factor (PWF) method show that the relationship between traffic volume and toll tariff is very sensitive, indicated by the elasticity value equal to 1. The difference between the two method is about 27% and is caused by the double counting on taxes on Jia method

Study of the relationship between arthropods and bacteria : molecular epidemiology and experimental models

Ce travail s’articule sur trois axes ; le premier est une contribution à l'étude du répertoire des bactéries associées aux arthropodes vecteurs (tique et puces) en Afrique du nord (Algérie) et en Afrique Sub-saharienne (Bénin, Tanzanie et République Démocratique du Congo). Nous avons pu ainsi détecter par biologie moléculaire (qPCRs, PCR standard et séquençage) et pour la première fois au Bénin, Rickettsia typhi (l'agent du typhus murin), et Bartonella sp dans des puces collectées sur des rongeurs à Cotonou. Dans ce travail, nous avons également détecté Yersinia pestis, l'agent de la peste et R. felis (responsable de la fièvre boutonneuse) dans des puces de la RD du Congo. En Tanzanie, nous avons mis en évidence la présence de R. felis et R. typhi dans des puces de rongeurs. En Algérie, nous avons décrit pour la première fois la présence d'agent de borréliose de Lyme (Borrelia garinii) dans les tiques. Nous avons confirmé la présence de R. massiliae, R. monacensis R. aeschlimannii, R. slovaca et R. felis et nous avons également détecté pour la première fois en Algérie, Bartonella tamiae, une bactérie dont la pathogénicité est peu connue et Coxiella burnetii, l'agent de la fièvre Q.Dans la deuxième partie de notre travail, nous nous sommes intéressés à l’évaluation des compétences vectorielles des puces de chat (Ctenocephalides felis) et punaises de lit (Cimex lectularius) dans la transmission de l’agent de la fièvre des tranchées (Bartonella quintana) dont le vecteur connu est le pou de corps. Trois approches ont été utilisées : la qPCR, la culture et l’immunohistochimie.This work focuses on three areas; the first is a contribution to the study of the repertoire of bacteria associated with arthropod vectors (tick and flea) in North Africa (Algeria) and in Sub-Saharan Africa (Benin, Tanzania and the Democratic Republic of Congo). We could thus detected by molecular tools (qPCRs, standard PCR and sequencing) and for the first time in Benin, Rickettsia typhi (the agent of murine typhus) and Bartonella sp in fleas collected from rodents in Cotonou. In this work, we have also associated the agent of plague (Yersinia pestis), and for the first time in fleas of DR of Congo, and we detected also R. felis (the causative agent of spotted fever). In Tanzania, we have highlighted the presence of R. typhi and R. felis fleas on rodents. In Algeria, we described for the first time the presence of Lyme disease agent (Borrelia garinii) in hard ticks. We confirmed the presence of R. massiliae, R. monacensis, R. aeschlimannii, R. slovaca and R. felis, we also detected for the first time Bartonella tamiae and Coxiella burnetii associated with bat ticks in Algeria.Regarding the second part we was interested in the evaluation of vector competence of cat fleas (Ctenocephalides felis) and bed bugs (Cimex lectularius) in the transmission of trench fever agent (Bartonella quintana) that is known to be transmitted by lice. Three approaches have been tested; qPCR, culture and immunohistochemistry

Emergence of mcr-1-mediated colistin resistance in Escherichia coli isolates from poultry in Algeria

International audienc

Assessment of oral ivermectin versus shampoo in the treatment of pediculosis (head lice infestation) in rural areas of Sine-Saloum, Senegal

International audienceReports of treatment failure and the emergence of resistance to topical head lice treatments have become increasingly common, driving the need for continued development of new therapeutic options for pediculosis. Ivermectin has been proposed as a potential alternative for the treatment of pediculosis but has not been sufficiently evaluated. In this study, the effectiveness of oral ivermectin versus shampoo in the treatment of pediculosis in Senegal was compared. The study was conducted in two neighbouring villages of Sine-Saloum, Senegal: Dielmo (ivermectin trial group; 201 female participants) and Ndiop (shampoo trial group; 239 female participants). In the ivermectin group, patients received two doses of oral ivermectin (400 mu g/kg body weight; Mectizan (R)) 7 days apart. In contrast, the shampoo group received a shampoo treatment based on D-phenothrin (0.23%; Hegor (R)). At the beginning of the study, 70 (34.8%) of 201 participants in the ivermectin group were infested by head lice versus 145 (60.7%) of 239 participants in the shampoo group. At Day 15 post-treatment, the efficacy of the treatment against head lice reached 41/53 (77.4%) in the ivermectin group (53 patients were tested in this group) versus 42/130 (32.3%) in the shampoo group (130 patients were tested in this group) (P < 10(-7)). However, 4 (7.5%) of the 53 females in the ivermectin group exhibited probable ivermectin treatment failure, suggesting the emergence of ivermectin-resistant lice. This study demonstrates that oral ivermectin is highly effective for the treatment of pediculosis compared with shampoo, but also suggests that ivermectin resistance may emerge during treatment. (C) 2016 Elsevier B.V. and International Society of Chemotherapy. All rights reserved

Prevalence of extended-spectrum beta-lactamase- and carbapenemase-encoding genes in poultry faeces from Algeria and Marseille, France

International audienceObjectives: The aim of this study was to evaluate the prevalence of extended-spectrum beta-lactamase (ESBL)- and carbapenemase-encoding genes in poultry from Algeria and Marseille, France. Methods: Samples consisted of faeces collected from broilers in France and Algeria between 2014 and 2015. DNA extraction and quantitative PCR were performed on 833 faecal samples to screen for the presence of genes encoding ESBLs (bla(TEM), bla(SHV) and bla(CTX-M)) and carbapenemases (blanom, bla(NDM), bla(VIM), bla(KPC), bla(OXA-23), bla(OXA-24), bla(OXA-48) and bla(OXA-)(58)). To characterise bacteria carrying antimicrobial resistance genes, positive results were simultaneously sequenced. Results: All chicken faeces from Marseille were negative for ESBL- and carbapenemase-encoding genes. However, of the 503 faecal samples collected in Algeria, 128 (25.4%) were positive for bla(TEM), 83 (16.5%) for bla(S)(HV), 46 (9.1%) for bla(CTX-M) and 132 (26.2%) for bla(OXA-)(58). Using a sequencing reaction, a high diversity of ESBL genes was observed throughout the sites studied. Conclusions: This study shows a high prevalence and diversity of ESBL and carbapenemase genes in poultry faeces from Algeria, whereas none of the samples from Marseille were positive for these genes. The high prevalence of ESBLs in Algeria is in contrast to the observation that no chickens were found to be positive in France. (C) 2017 International Society for Chemotherapy of Infection and Cancer. Published by Elsevier Ltd. All rights reserved

Detection of Rickettsia hoogstraalii, Rickettsia helvetica, Rickettsia massiliae, Rickettsia slovaca and Rickettsia aeschlimannii in ticks from Sardinia, Italy

International audienceTick-borne diseases represent a large proportion of infectious diseases that have become a world health concern. The presence of Rickettsia spp. was evaluated by standard PCR and sequencing in 123 ticks collected from several mammals and vegetation in Sardinia, Italy. This study provides the first evidence of the presence of Rickettsia hoogstralii in Haemaphysalis punctata and Haemaphysalis sulcata ticks from mouflon and Rickettsia helvetica in Ixodes festai ticks from hedgehog. In addition, Rickettsia massiliae, Rickettsia slovaca and Rickettsia aeschlimannii were detected in Rhipicephalus sanguineus, Dermacentor marginatus and Hyalomma marginatum marginatum ticks from foxes, swine, wild boars, and mouflon. The data presented here increase our knowledge of tick-borne diseases in Sardinia and provide a useful contribution toward understanding their epidemiology. (C) 2016 Elsevier GmbH. All rights reserved

Molecular evidence of tick-borne hemoprotozoan-parasites (Theileria ovis and Babesia ovis) and bacteria in ticks and blood from small ruminants in Northern Algeria

International audienceUsing qPCR, standard PCR and/or sequencing, we investigated the presence of tick- associated microorganisms in ticks and blood from sheep and goats from Souk Ahras, Algeria. Borrelia theileri, was detected in (7/120, 5.8%) blood from sheep and (13/120, 10.8%) goats. Anaplasma ovis was screened in (38/73, 52%) Rhipicephalus bursa and (5/22, 22.7%) R. turanicus and in (74/120, 61.7%), (65/120, 54.2%) blood of sheep and goats respectively. Coxiella burnetii tested positive in R. bursa (4/73, 5.5%) and (7/120, 5.8%) blood of sheep and (2/120, 1.7%) goats. Theileria ovis was detected in (50/147, 34%) R. bursa and (3/22, 13.6%) R. turanicus and in (64/120, 53.3%) blood of sheep and (25/120, 20.8%) goats. Babesia ovis was screened positive in (23/147, 15.6%) R. bursa and (7/48, 14.6%) R. turanicus. Our findings expand knowledge about the repertoire of tick- borne microorganisms present in ectopar-asites and/or the blood of small ruminants in Algeria. (C) 2016 Elsevier Ltd. All rights reserved

Molecular Evidence of Rickettsia slovaca in Wild Boar Lice, in Northeastern Algeria

International audienceUsing molecular assays, Rickettsia slovaca, the agent of a spotted fever group rickettsia was assessed in 92/325 lice collected on 117 wild boars hunted in the far northeast of Algeria. Overall, 7/92 (7.61%) Haematopinus suis were found positive to R. slovaca. The presence of R. slovaca DNA on H. suis questions about the relationship that may exist between R. slovaca and lice

Detection of Bartonella tamiae, Coxiella burnetii and rickettsiae in arthropods and tissues from wild and domestic animals in northeastern Algeria

International audienceBackground: In recent years, the scope and importance of emergent vector-borne diseases has increased dramatically. In Algeria, only limited information is currently available concerning the presence and prevalence of these zoonotic diseases. For this reason, we conducted a survey of hematophagous ectoparasites of domestic mammals and/or spleens of wild animals in El Tarf and Souk Ahras, Algeria. Methods: Using real-time PCR, standard PCR and sequencing, the presence of Bartonella spp., Rickettsia spp., Borrelia spp. and Coxiella burnetii was evaluated in 268/1626 ticks, 136 fleas, 11 Nycteribiidae flies and 16 spleens of domestic and/or wild animals from the El Tarf and Souk Ahras areas. Results: For the first time in Algeria, Bartonella tamiae was detected in 12/19 (63.2 %) Ixodes vespertilionis ticks, 8/11 (72.7 %) Nycteribiidae spp. flies and in 6/10 (60 %) bat spleens (Chiroptera spp.). DNA from Coxiella burnetii, the agent of Q fever, was also identified in 3/19 (15.8 %) I. vespertilionis from bats. Rickettsia slovaca, the agent of tick-borne lymphadenopathy, was detected in 1/1 (100 %) Haemaphysalis punctata and 2/3 (66.7 %) Dermacentor marginatus ticks collected from two boars (Sus scrofa algira) respectively. Ri. massiliae, an agent of spotted fever, was detected in 38/94 (40.4 %) Rhipicephalus sanguineus sensu lato collected from cattle, sheep, dogs, boars and jackals. DNA of Ri. aeschlimannii was detected in 6/20 (30 %) Hyalomma anatolicum excavatum and 6/20 (30 %) Hy. scupense from cattle. Finally, Ri. felis, an emerging rickettsial pathogen, was detected in 80/110 (72.7 %) Archaeopsylla erinacei and 2/2 (100 %) Ctenocephalides felis of hedgehogs (Atelerix algirus). Conclusion: In this study, we expanded knowledge about the repertoire of ticks and flea-borne bacteria present in ectoparasites and/or tissues of domestic and wild animals in Algeria