Protein kinases and phosphatases regulating the yeast proton pump

[EN] The plasma membrane H+-ATPase (Pma1) is essential for yeast growth and is activated by glucose metabolism by an unknown mechanism involving double phosphorylation of a regulatory site at the C-terminus (Ser911 Thr912). In this thesis we have investigated in Saccharomyces cerevisiae the role of two protein phosphatases, type 1 Glc7 and type 2A Sit4, and of an essential atypical protein kinase, TORC1, in the activation of Pma1 by glucose. The regulatory site of activated Pma1 can be dephosphorylated "in vitro" by recombinant Glc7 and Sit4, but inhibition "in vivo" of these phosphatases does not activate Pma1. Inhibition of Glc7 by regulated expression of a dominant-negative truncated form (the null mutant is not viable) had no effect on Pma1 activity while deletion of SIT4 gene decreased both Pma1 activity and double phosphorylation of the regulatory site. Inhibition of TORC1 protein kinase by treatment of yeast cells with the drug rapamycin or by exposure to non-permissive temperature of a temperature-sensitive mutant (tor1¿ tor2ts) inhibited Pma1 and decreased double phosphorylation of the regulatory site. We conclude that Sit4 and TORC1 are required for full activation of Pma1 by glucose while Glc7 either does not participate or is redundant with other phosphatases.[ES] La H+-ATPasa de la membrana plasmática (Pma1) es esencial para el crecimiento de la levadura y se activa por metabolismo de glucosa por un mecanismo desconocido que lleva consigo la doble fosforilación de un sitio regulador en el extremo C-terminal (Ser911 Thr912). En la presente tesis hemos investigado en Saccharomyces cerevisiae la participación de dos proteína fosfatasas, Glc7 de tipo 1 y Sit4 de tipo 2A, y de una proteína kinasa atípica esencial, TORC1, en la activación de Pma1 por glucosa. El sitio regulador de Pma1 en su estado activo puede defosforilarse "in vitro" por Glc7 y Sit4 recombinantes pero la inhibición "in vivo" de estas fosfatasas no activa Pma1. La inhibición de Glc7 mediante la expresión regulada de una forma truncada que actúa como dominante-negativa (el mutante nulo no es viable) no tiene efecto en la actividad de Pma1 mientras que la deleción del gen SIT4 disminuye tanto la actividad de Pma1 como la doble fosforilación del sitio regulador. Inhibición de la proteína kinasa TORC1 mediante tratamiento de las células de levadura con el fármaco rapamicina o exponiéndolas a temperatura no permisiva en el caso de un mutante termosensible (tor1¿ tor2ts) resulta en inhibición de Pma1 y disminución de la doble fosforilación del sitio regulador. Estos resultados indican que Sit4 y TORC1 son necesarias para la máxima activación de Pma1 por glucosa mientras que Glc7 podría no participar o hacerlo de forma redundante con otras fosfatasas.[CA] L'H+-ATPasa de la membrana plasmàtica (Pma1) és essencial per al creixement dels llevats i s'activa gràcies al metabolisme de glucosa per un mecanisme desconegut que porta associat la doble fosforilació d'una regió reguladora a l'extrem C-terminal (Ser911 Thr912). En aquesta tesi hem investigat en Saccharomyces cerevisiae la participació de dos proteïnes fosfatases, Glc7 de tipus 1 i Sit4 de tipus 2A, i d'una proteïna quinasa essencial atípica, TORC1, en l'activació de Pma1 per glucosa. La regió reguladora de Pma1, en seu estat activat, pot desfosforar-se "in vitro" per Glc7 i Sit4 recombinants, però la inhibició "in vivo" d'aquestes fosfatases no activa Pma1. La inhibició de Glc7 mitjançant l'expressió regulada d'una forma truncada que actua com a dominant-negativa (el mutant nul no és viable) no té cap efecte en l'activitat de Pma1 mentre que la deleció del gen SIT4 disminueix tant l'activitat de Pma1 com la doble fosforilació de la regió reguladora. La inhibició de la proteïna quinasa TORC mitjançant un tractament de cèl·lules de llevat amb el fàrmac rapamicina o la seua exposició a temperatures no permissives en el cas d'un mutant termosensible (tor1¿ tor2ts) resulta en la inhibició de Pma1 i la disminució de la doble fosforilació de la regió reguladora. Aquests resultats indiquen que Sit4 i TORC1 són necessàries per a l'activació màxima de Pma1 per glucosa, mentre que Glc7 podria no participar o fer-ho d'una forma redundant amb altres fosfatases.Mahmoud Ali Ibrahim Hamouda, S. (2015). Protein kinases and phosphatases regulating the yeast proton pump [Tesis doctoral no publicada]. Universitat Politècnica de València. https://doi.org/10.4995/Thesis/10251/54131TESI

Modeling and Simulation of Switched Reluctance Machines

This chapter discusses the modeling and simulation approaches for switched reluctance machines (SRMs). First, it presents the modeling methods for SRMs including analytical models, Artificial intelligence based models, and lookup tables based models. Furthermore, it introduces the finite element method (FEM) and experimental measurement methods to obtain high fidelity magnetic characteristics for SRMs. Step-by-step procedure is explained for SRM modeling and analysis using FEM. The direct and indirect measurement methods of SRM magnetic characteristics are included, comparison between the measured and FEM-calculated characteristics is achieved, and good agreement is seen. In addition, this chapter gives the mathematical modeling of SRM, and explains its model development using MATLAB/Simulink environment. Simulation and experimental results are obtained, a very good agreement is observed

Numerical estimation of switched reluctance motor excitation parameters based on a simplified structure average torque control strategy for electric vehicles

Switched reluctance motors (SRMs) have been receiving great attention in electric vehicle (EV) applications. However, the complicated control and inherent torque ripples are the major drawbacks of SRMs. This paper introduces a numerical estimation method for the optimum control parameters of SRM based on a simplified average torque control (ATC) strategy for EVs. The proposed control aims to simplify the control algorithm to cut down complexity and cost. Besides, it aims to achieve all the vehicle requirements. A multi-objective optimization problem is set to determine the most efficient excitation parameters that can fulfill the vehicle requirements. The objective function has two terms: torque ripple and efficiency. Proper constraints for both turn-on and turn-off angles are included in order to achieve high-performance control, maximum torque per Ampere (MTPA) production, and reliable operation. Besides, additional toque constraints are involved to ensure fast dynamics, high-performance torque tracking capability, and parameter insensitivity. The motor model is accurately achieved based on the experimentally measured torque and flux characteristics. Several simulations are executed to prove the feasibility and effectiveness of the proposed control. Moreover, experimental results are obtained to validate the theoretical findings. It is observed that the proposed control has a significant reduction of torque ripples compared to the conventional control methods. The average reduction ratio of torque ripple over the speed range is about 72.43%. Besides, the proposed control succeeds in maintaining a very good efficiency and high torque/current ratio. It also has a fast-dynamic performanc

Comparative evaluation for an improved direct instantaneous torque control strategy of switched reluctance motor drives for electric vehicles

Due to the expected increase in the electric vehicles (EVs) sales and hence the increase of the price of rare-earth permanent magnets, the switched reluctance motors (SRMs) are gaining increasing research interest currently and in the future. The SRMs offer numerous advantages regarding their structure and converter topologies. However, they suffer from the high torque ripple and complex control algorithms. This paper presents an improved direct instantaneous torque control (DITC) strategy of SRMs for EVs. The improved DITC can fulfill the vehicle requirements. It involves a simple online torque estimator and a torque error compensator. The turn-on angle is defined analytically to achieve wide speed operation and maximum torque per ampere (MTPA) production. Moreover, the turn-off angles are optimized for minimum torque ripples and the highest efficiency. In addition, this paper provides a detailed comparison between the proposed DITC and the most applicable torque control techniques of SRMs for EVs, including indirect instantaneous torque control (IITC), using torque sharing function (TSF) strategy and average torque control (ATC). The results show the superior performance of the proposed DITC because it has the lowest torque ripples, the highest torque tor current ratio, and the best efficiency over the low and medium speed ranges. Moreover, the comparison shows the advantages of each control technique over the range of speed control. It provides a very clear overview to develop a universal control technique of SRM for EVs by merging two or more control techniques

An improved indirect instantaneous torque control strategy of switched reluctance motor drives for light electric vehicles

The switched reluctance motors (SRMs) are powerful alternatives for electric vehicles (EVs). However, the high torque ripple is the main obstacle for their acceptance in high-performance applications. This paper introduces an improved indirect instantaneous torque control (IITC) strategy of SRMs for EVs. It aims to achieve the vehicle requirements including maximum torque per ampere (MTPA), minimum torque ripple, high efficiency, and extended speed range. First, a simple analytical formulation that determines the most efficient turn-on angle for torque production is developed. Second, A modified torque sharing function (TSF) is introduced to compensate for torque tracking errors. To accurately represent the SRM, its magnetic characteristics are calculated using finite element method (FEM). They are employed to build machine model and implement the required transformations. Finally, the particle swarm optimization (PSO) algorithm is adopted to determine the best control parameters for the conventional IITC. This is done basically for comparison and verification purposes. The results show the feasibility and effectiveness of the proposed control over extended speed range

Management of tibioperoneal trunk aneurysm in a patient with Behçet disease.

Only a few cases of infrapopliteal aneurysms are reported in the literature. These are commonly associated with trauma, infection, and iatrogenic injuries and mostly present as pseudoaneurysms. We report the case of a 44-year-old man with Behçet disease and an 8-cm tibioperoneal trunk aneurysm and discuss the management options of these aneurysms

Assessment of Emissions from Cement Plants Using AERMOD Modeling

Coal combustion generates many gases and emissions which are harmful to public health and the environment. So, it is necessary to assess the health risks for the people living in the nearby cement plants that use coal as a fuel. In this article, a health risk assessment HRA was carried out concerning the air emissions from a cement plant in the heavy industry area located at Beni Suef governorate - Egypt. The article handles the assessment of the health risks for pollutants classified as non-carcinogenic i.e. sulfur dioxide - mercury and the health risks for pollutants classified as carcinogenic i.e. arsenic – chromium VI. An Air dispersion modeling program AERMOD is used to measure and evaluate long and short terms health impacts to expect the concentration of pollutants at the ground level within 30 km radius of the studied cement plant. The emissions measurements findings are used as input to the model in addition to some factors such as meteorology and surrounding terrain. Consequently, the program can implement simulations for the emissions concentration level of the mentioned pollutants and their effects on the population at Jazirat Abu Salih village, which is 10 km far from the studied cement plant. The results for mentioned pollutants concentrations levels matched with acceptance and safe levels of ambient air quality standards. In addition, the increment lifetime cancer risk ILCR by inhalation was calculated for arsenic and chromium and all results conformed with the safe and accepted limits

Medical prospects of cryptosporidiosis in vivo control using biofabricated nanoparticles loaded with Cinnamomum camphora extracts by Ulva fasciata

Background and Aim: Global efforts are continuing to develop preparations against cryptosporidiosis. This study aimed to investigate the efficacy of biosynthesized Ulva fasciata loading Cinnamomum camphora oil extract on new zinc oxide nanoparticles (ZnONPs shorten to ZnNPs) and silver nanoparticles (AgNPs) as alternative treatments for Cryptosporidium parvum experimental infection in rats. Materials and Methods: Oil extract was characterized by gas chromatography-mass spectrometry, loaded by U. fasciata on ionic-based ZnO and NPs, and then characterized by transmission electron microscopy, scanning electron microscopy, and X-ray diffraction. Biosafety and toxicity were investigated by skin tests. A total of 105 C. parvum oocysts/rat were used (n = 81, 2–3 W, 80–120 g, 9 male rats/group). Oocysts shedding was counted for 21 d. Doses of each preparation in addition to reference drug were administered daily for 7 d, starting on post-infection (PI) day (3). Nitazoxanide (100 mg) was used as the reference drug. After 3 weeks, the rats were sacrificed for postmortem examination and histopathological examination. Two blood samples/rat/group were collected on the 21st day. Ethylenediaminetetraacetic acid blood samples were also used for analysis of biochemistry, hematology, immunology, micronucleus prevalence, and chromosomal abnormalities. Results: C. camphora leaves yielded 28.5 ± 0.3 g/kg oil and 20 phycocompounds were identified. Spherical and rod-shaped particles were detected at 10.47–30.98 nm and 18.83–38.39 nm, respectively. ZnNPs showed the earliest anti-cryptosporidiosis effect during 7–17 d PI. Other hematological, biochemical, immunological, histological, and genotoxicity parameters were significantly fruitful; hence, normalized pathological changes induced by infestation were observed in the NPs treatments groups against the infestation-free and Nitazoxanide treated group. Conclusion: C. camphora, U. fasciata, ZnNPs, and AgNPs have refluxed the pathological effects of infection as well as positively improved host physiological condition by its anticryptosporidial immunostimulant regenerative effects with sufficient ecofriendly properties to be proposed as an alternative to traditional drugs, especially in individuals with medical reactions against chemical commercial drugs