Epigenetic marks as the link between environment and development: examination of the associations between attachment, socioeconomic status, and methylation of the SLC6A4 gene.

BackgroundEpigenetic processes act as a link between environment and individual development. This pilot study examined the association between socioeconomic status (SES), attachment, and methylation of the promoter region of the serotonin transporter gene (SLC6A4).MethodsAttachment classification and SLC6A4 methylation was determined in 100 late adolescents. We hypothesized that (1) SES would interact with methylation to predict higher unresolved loss (UL) or trauma scores on the Adult Attachment Interview; (2) across SES, participants with unresolved attachment would have lower levels of methylation than organized or secure participants; and (3) within the unresolved classification, SES would predict methylation.ResultsResults showed that lower methylation and low-SES were associated with higher UL, and higher methylation and low-SES were associated with higher unresolved trauma. Across SES, unresolved participants had lower levels of methylation than organized participants. Within the unresolved category, low-SES unresolved participants had higher levels of methylation than mid/upper-SES participants. SES was unrelated to methylation within the secure and organized categories.ConclusionsThese results suggest that the quality of attachment relationships may impact epigenetic processes

Life story approaches and relationships within residential child care : a practice reflection

Residential child care creates a world of opportunity. It is unique and dynamic and often helps shape and direct the future for many children and young people. Relationships are key, they are multi-layered and adaptable, moulding to the children/young people and their individual needs. The use of daily life events, the rhythms, rituals and routines of the house help to create a safe space where the journey of therapeutically exploring the life story can evolve. In this article the author uses a case study to provide the reader with experiential examples, highlighting the importance of these every day interactions and life events to build and sustain authentic relationships within the residential child care environment, demonstrating the impact of such relationships in supporting a young person’s exploration of their life story. The recognition that life story work is often a process and not a specific task allows the residential practitioner to consider their role in this journey as adaptable and helps to identify greater opportunities for relationships to be enhanced

COVID-19, the journey from crisis to opportunity : experiences of young people in residential child care and their carers

COVID-19 arrived as a crisis. Its impact has been felt across the Globe and will continue to be for many years to come. Financially, emotionally, practically and psychologically – it has changed many views & forced us to think and behave differently in our everyday life. A massive challenged faced residential child care when lockdown was announced. Fear swept through the house, as the reality of our young people experiencing another challenge gripped us. At Nether Johnstone House, we have an ethos built around opportunity, experiences, relationships and most importantly love. In this article, our young people and team share some of our reflections and learnings of lockdown. Time has never seemed more important or significant than it has throughout 2020 and we have grown to appreciate this in its simplest form

Necrotizing Periodontal Diseases in a Semirural District of South Africa

Objectives. The aim of this study was to characterize the lesions of necrotizing gingivitis (NG) and necrotizing periodontitis (NP) with regard to extent and severity, and to correlate these parameters with the host HIV serostatus, CD4+ T-cell count, neutrophil count, age, and gender. Methods. Eighty-four consecutive patients, 39 black females and 45 black males aged 20–46 years, diagnosed with NG/NP were recruited to the study over a period of two years. Results. For both HIV-seropositive and -seronegative patients, the mandibular anterior gingiva was most frequently affected; 74% had NG/NP affecting ≥5 gingival tooth sites. Ninety percent of all patients had a mean severity of ≤4 mm. There was no statistically significant association between either extent or severity of NG/NP and HIV serostatus, CD4+ T-cell count, neutrophil count, age, or gender. The difference between the number of HIV-seropositive patients with NG/NP who had CD4+ T-cell counts ≤200 cells/mm3 and those who had CD4+ T cell counts of 201–499 cells/mm3 was not statistically significant. Conclusion. The clinical signs of NG/NP are similar in HIV-seropositive and -seronegative patients, and are not related to CD4+ T-cell count, to neutrophil count, to gender, or to age

On the interaction between human IQGAP1 and actin

DM thanks the School of Biological Sciences, Queen’s University, Belfast for a summer studentship and EH thanks the Department of Employment and Learning, Northern Ireland for a postgraduate studentship. The work was funded in part by grants from the BBSRC (BB/D000394/1 To DJT) and by the Wellcome Trust [grant number GR06281AIA] which funded the purchase of the QStar XL mass spectrometer at the BBSRC Mass Spectrometry and Proteomics Facility, University of St Andrews and funded SLS.IQGAPs are eukaryotic proteins which integrate signals from various sources and pass these on the cytoskeleton. Understanding how they do this requires information on the interfaces between the proteins. Here, it is shown that the calponin homology domain of human IQGAP1 (CHD1) can be crosslinked with α-actin. The stoichiometry of the interaction was 1:1. A molecular model was built of the complex and associated bioinformatics analyses predicted that the interaction is likely to involve an electrostatic interaction between Lys-240 of α-actin and Glu-30 of CHD1. These residues are predicted to be accessible and are not involved in many intra-protein interactions; they are thus available for interaction with binding partners. They are both located in regions of the proteins which are predicted to be flexible and disordered; interactions between signalling molecules often involve flexible, disordered regions. The predicted binding region in CHD1 is well conserved in many eukaryotic IQGAP-like proteins. In some cases (e.g Dictyostelium discoideum and Saccharomyces cerevisiae) protein sequence conservation is weak, but molecular modelling reveals that a region of charged, polar residues in a flexible N-terminus is structurally well conserved. Therefore we conclude that the calponin homology domains of IQGAP1-like proteins interact initially through the electrostatic interaction identified here and that there may be subsequent conformational changes to form the final complex.PostprintPeer reviewe

Shared Modular Course Development: A Feasibility Study

This project evaluated the viability of shared course development (SCD) and identified the necessary baseline mechanisms, principles, policies, and procedures for future joint course development collaborations. Although collaborative course design is still relatively new in Ontario, our institutionally-based project teams identified and researched a number of successful examples from Australia, Canada, Europe, New Zealand, the United Kingdom, and the United States. These successful models demonstrated the transformative possibilities of blended learning, expanded course variety, maintained or enhanced the breadth of course offerings, and reduced institution-specific development costs while maintaining delivery autonomy. They also focused on enhancing student learning and produced momentum for instructional improvement and course re-design among collaborating institutions. This report concludes that there is considerable value to the development of collaborative institutional cultures in and of itself, and that collaborative capacity will become an increasingly important core competency in the more differentiated and change-oriented university sector that is emerginghttps://scholar.uwindsor.ca/ctlreports/1000/thumbnail.jp

Analysis of four DLX homeobox genes in autistic probands

BACKGROUND: Linkage studies in autism have identified susceptibility loci on chromosomes 2q and 7q, regions containing the DLX1/2 and DLX5/6 bigene clusters. The DLX genes encode homeodomain transcription factors that control craniofacial patterning and differentiation and survival of forebrain inhibitory neurons. We investigated the role that sequence variants in DLX genes play in autism by in-depth resequencing of these genes in 161 autism probands from the AGRE collection. RESULTS: Sequencing of exons, exon/intron boundaries and known enhancers of DLX1, 2, 5 and 6 identified several nonsynonymous variants in DLX2 and DLX5 and a variant in a DLX5/6intragenic enhancer. The nonsynonymous variants were detected in 4 of 95 families from which samples were sequenced. Two of these four SNPs were not observed in 378 undiagnosed samples from North American populations, while the remaining 2 were seen in one sample each. CONCLUSION: Segregation of these variants in pedigrees did not generally support a contribution to autism susceptibility by these genes, although functional analyses may provide insight into the biological understanding of these important proteins

Collection of Articles and Reviews on the Miss Cora Story

Collection of different articles by different writers on Cora Wilson Stewart