Ultrastructure of Embryonic Phaseolus Vulgaris L. Axes During Anhydrobiosis and the Development of Mitochondria During Early Germination

Botan

Acción colectiva, gestión de recursos hídricos y adaptación al cambio climático en la microcuenca de Cuspire, Jinotega, 2012

La presente investigación se realizó en la microcuenca de Cuspire, perteneciente a la subcuenca del Río Yalí, municipio de Jinotega; con el objetivo de valorar la acción colectiva para la gestión de recursos hídricos y adaptación al cambio climático; proporcionando alternativas de solución a los pobladores para el buen uso y manejo al agua. Empleando el enfoque cuantitativo, carácter descriptivo, corte transversal y no experimental. La recopilación de datos fue por medio de la aplicación de 108 encuestas como muestra, a una población de 477 viviendas, distribuidas en las ocho comunidades (La Naranjita, La Bolsa, El Volcán, Las Trozas, Prendedizos, Buena Vista, Las Quiatas y Estrechura) pertenecientes a la zona de estudio. Como principales resultados se obtuvo, que existe acción colectiva para consumo humano, alimentos y usos domésticos y no así en agricultura de regadío, ganadería y agroindustria. La comunidad más destacada en gestionar el agua de manera conjunta es Buena Vista, ya que los pobladores toman las decisiones de manera colectiva incidiendo de forma positiva en el funcionamiento del sistema; como menos destacada se encuentra la comunidad Las Quiatas por poseer mayor incidencia de conflictos, debido a que la mayoría de la población se abastece a través de puestos públicos. Todos los habitantes expresaron haber sufrido los efectos provocados por el cambio climático (sequías y lluvias), sin contar con un plan estratégico para mitigarlos; al tomar las medidas para reducir estos efectos, las familias de Prendedizos buscaron alternativas mediante acuerdos con otras familias, mientras que la comunidad Buena Vista lo hizo de forma aislad

The Freshman, vol. 5, no. 3

The Freshman was a weekly, student newsletter issued on Mondays throughout the academic year. The newsletter included calendar notices, coverage of campus social events, lectures, and athletic teams. The intent of the publication was to create unity, a sense of community, and class spirit among first year students

The Freshman, vol. 5, no. 3 [issue misnumbered]

The Freshman was a weekly, student newsletter issued on Mondays throughout the academic year. The newsletter included calendar notices, coverage of campus social events, lectures, and athletic teams. The intent of the publication was to create unity, a sense of community, and class spirit among first year students. Included in this edition is an editorial authored on behalf of The Co-eds of \u2738 commenting on the divisive nature of Freshman Rules imposed by upper classmen and requesting first-year students be treated with respect

Clinical and basic implications of dynamic T cell receptor clonotyping in hematopoietic cell transplantation

TCR repertoire diversification constitutes a foundation for successful immune reconstitution after allogeneic hematopoietic cell transplantation (allo-HCT). Deep TCR V beta sequencing of 135 serial specimens from a cohort of 35 allo-HCT recipients/donors was performed to dissect posttransplant TCR architecture and dynamics. Paired analysis of clonotypic repertoires showed a minimal overlap with donor expansions. Rarefied and hyperexpanded clonotypic patterns were hallmarks of T cell reconstitution and influenced clinical outcomes. Donor and pretransplant TCR diversity as well as divergence of class I human leukocyte antigen genotypes were major predictors of recipient TCR repertoire recovery. Complementary determining region 3-based specificity spectrum analysis indicated a predominant expansion of pathogen- and tumor-associated clonotypes in the late post-allo-HCT phase, while autoreactive clones were more expanded in the case of graft-versus-host disease occurrence. These findings shed light on post-allo-HCT adaptive immune reconstitution processes and possibly help in tracking alloreactive responses

Biomarker Panel for Chronic Graft-Versus-Host Disease

PURPOSE: To identify diagnostic and prognostic markers of chronic graft-versus-host disease (cGVHD), the major cause of morbidity and mortality after allogeneic hematopoietic cell transplantation (HCT). PATIENTS AND METHODS: Using a quantitative proteomics approach, we compared pooled plasma samples obtained at matched time points after HCT (median, 103 days) from 35 patients with cGVHD and 18 without cGVHD (data are available via ProteomeXchange with identifier PXD002762). Of 105 proteins showing at least a 1.25-fold difference in expression, 22 were selected on the basis of involvement in relevant pathways and enzyme-linked immunosorbent assay availability. Chemokine (C-X-C motif) ligand 9 (CXCL9) and suppression of tumorigenicity 2 (ST2) also were measured on the basis of previously determined associations with GVHD. Concentrations of the four lead biomarkers were measured at or after diagnosis in plasma from two independent verification cohorts (n = 391) to determine their association with cGVHD. Their prognostic ability when measured at approximately day +100 after HCT was evaluated in plasma of a second verification cohort (n = 172). RESULTS: Of 24 proteins measured in the first verification cohort, nine proteins were associated with cGVHD, and only four (ST2, CXCL9, matrix metalloproteinase 3, and osteopontin) were necessary to compose a four-biomarker panel with an area under the receiver operating characteristic curve (AUC) of 0.89 and significant correlation with cGVHD diagnosis, cGVHD severity, and nonrelapse mortality. In a second verification cohort, this panel distinguished patients with cGVHD (AUC, 0.75), and finally, the panel measured at day +100 could predict cGVHD occurring within the next 3 months with an AUC of 0.67 and 0.79 without and with known clinical risk factors, respectively. CONCLUSION: We conclude that the biomarker panel measured at diagnosis or day +100 after HCT may allow patient stratification according to risk of cGVHD