Hyperthermia effect on human normal breast (MCF-10A) and cancer (MDA-MB 231 and MCF-7) cells

Objective: In this study, the hyperthermia effect on the viability of human normal breast (MCF-10A) and cancer (MDA-MB 231 and MCF-7) cells was evaluated by MTT assay. Methods: Cells were exposed to heat at 38ºC, 39ºC, 40ºC, 41ºC, 42ºC, 43ºC, and 44ºC for five different durations of heat exposure (0.5, 1, 2, 3, and 4 h). Breakpoint temperatures of MCF-10A, MDA-MB 231, and MCF-7 were determined using cumulative equivalent 43°C (CEM43) model. This model was first time used to calculate thermal isoeffect dose (TID) for MCF-10A, MDA-MB 231, and MCF-7. Results: MCF-10A started to die at 42°C for 3 h while MDA-MB 231 and MCF-7 need a temperature of 38°C for 0.5 h; thus, they were identified as the threshold temperatures in CEM43 model. Furthermore, the effect of “43°C incubator 2 h” had similar total thermal dose as “44°C incubator 0.5 h” for MDA-MB 231 and MCF-7. In addition, “43°C incubator 3 h” effect had also almost the same thermal dose as “44°C incubator 1 h” for MDA-MB 231 and MCF-7. Conclusion: A better understanding of the significant correlations between CEM43 and response parameters in clinical trials could be useful to treat breast cancer patients

The Effects of Zeolite X and Y on Cancer Cell Lines

Zeolites are hydrated silicates of aluminium that have been very useful in many industry because of its microporous property, absorbance ability and ion exchange capacity. It is currently viewed as a potential adjuvant in cancer therapy due to its ability to inhibit the proliferation of cancer cells. Research on natural zeolite clinoptilolite application as anticancer agent has been proven by others. However, the effect of other types of zeolite on cancer cells is still uncertain. This study is performed to determine the effects of zeolite X and Y on cancer cell lines proliferation in vitro. Cancer cell lines HeLa, AsPC-1 and 911 cells were cultured in designated medium treated with zeolite X and zeolite Y at the concentration of 5 mg/ml and 50 mg/ml. Fetal Bovine Serum (FBS) concentrations were modified to 5%, 10%, 15% and 20%. After 72 hours incubation, the efficacy of zeolite to treat cancer cell lines were measured by means of cell viability test via MTT assay. Overall results showed that cancer cell lines cultivated in the medium treated with 50 mg/ml of zeolite X and 5% FBS exhibited the highest inhibition of cell proliferation and decrease in cell viability. This finding provides preliminary information in the study of determining the potential use of zeolite as anticancer agent for alternative or complementary therapy

Effect of surfactant modified clinoptilolite added propagating substrate in the growth of clinancathus nutans

Nitrogen (N), phosphorus (P) and potassium (K) nutrients are important for plant growth especially for herbal plants. N is essential for leaf growth, P is crucial for rooting development of the plants while K is important for fruit and flower development of plants. Zeolite (clinoptilolite) cannot adsorb anions such as phosphate (PO43-) and nitrate (NO3-) which vital for the plant growth because of its negative charge. Hence, an experiment was carried out to modify clinoptilolite with cationic surfactant, hexadecyltrimethylammonium-bromide (HDTMA-Br) producing surfactant modified clinoptilolite (SMC) which later then added to propagating substrate to form controlled release propagating substrate (CRPS) which can hold and control the anion and cation release. The release behaviour of PO43-, NO3- and K? from CRPS was studied for ten days. Results showed that, the combination of SMC with 50 g propagating substrate could control the anions and cation release and furthermore enhance the growth of Clinacanthus nutans during sowing time. Therefore, SMC is needed to be added in propagating substrate to control the release of nutrients during germination or sowing time

Investigation of porcupine bezoar extract combined with electroporation on HeLa cell

Current chemo-preventive agents will causes a long term side effects in cancer sufferer. Therefore, this research focusses on the benefits of combine both technique of Electroporation (EP) method and with natural animal extract in process to inhibit the proliferation of cancer cell, as a solution to reduce or adverse effects of orthodox drugs. The present study indicates on anti-cancer potentially of Porcupine Bezoar Extract (PBE), also known as Hystrix Brachyuran against HeLa cell. Cell viability of HeLa cell were determined after HeLa cell treated with 500 V/cm and pulse duration of 100µs before the concentration of 80.0 µg/ml of Porcupine Bezoar Extract (PBE) was added into the cell. The cell viability and cell growth were monitored up to 48 hours with comparative to untreated cell as a control group.This study result proved that by combine both technique; Electroporation (EP) and with natural animal extract Porcupine Bezoar Extract (PBE) might open the door and it has an ability in supressing the growth of HeLa cell

Antibacterial activity of CTAB-modified zeolite NaY with different CTAB loading

The antibacterial activity of cetyltrimethyl ammonium bromide (CTAB) –Modified Zeolite NaY against Escherichia coli and Staphylococcus aureus was examined in agar disk diffusion (Kirby-Bauer) method. The diameter of the inhibition zones for E. coli and S. aureus, increased from 1.5 to 1.7 cm and 1.9 to 2.0 cm, respectively, with the increasing concentrations of CTAB adsorbed on Zeolite NaY from percent coverage of the External Cation Exchange Capacity of Zeolite NaY: 0.5 to 5.0. CTAB-Modified Zeolite NaY showed effective antimicrobial activity against S. aureus compared to E. coli

Stimulated Ag nanoparticles by pulsed laser ablation for breast cancer treatment

N

Alleviation of diabetic nephropathy by zinc oxide nanoparticles in streptozotocin‐induced type 1 diabetes in rats

Abstract This study examines the effect of nanoparticles with zinc oxides (ZnONPs) on diabetic nephropathy, which is the primary cause of mortality for diabetic patients with end‐stage renal disease. Diabetes in adult male rats was induced via intraperitoneal injection of streptozotocin. ZnONPs were intraperitoneally administered to diabetic rats daily for 7 weeks. Diabetes was associated with increases in blood glucose level, 24‐h urinary albumin excretion rate, glomerular basement membrane thickness, renal oxidative stress markers, and renal mRNA or protein expression of transforming growth factor‐β1, fibronectin, collagen‐IV, tumour necrosis factor‐α and vascular endothelial growth factor‐A. Moreover, the expression of nephrin and podocin, and the mRNA expression of matrix metalloproteinase‐9 were decreased in the diabetic group. These changes were not detected in the control group and were significantly prevented by ZnONP treatment. These results provide evidence that ZnONPs ameliorate the renal damage induced in a diabetic rat model of nephropathy through improving renal functionality; inhibiting renal fibrosis, oxidative stress, inflammation and abnormal angiogenesis; and delaying the development of podocyte injury. The present findings may help design the clinical application of ZnONPs for protection against the development of diabetic nephropathy

Pharmacological Mechanisms Underlying Gastroprotective Activities of the Fractions Obtained from Polygonum minus in Sprague Dawley Rats

The leaves of Polygonum minus were fractionated using an eluting solvent to evaluate the pharmacological mechanisms underlying the anti-ulcerogenic activity of P. minus. Different P. minus fractions were obtained and evaluated for their ulcer preventing capabilities using the ethanol induction method. In this study, Sprague Dawley rats weighing 150–200 g were used. Different parameters were estimated to identify the active fraction underlying the mechanism of the gastroprotective action of P. minus: the gastric mucus barrier, as well as superoxide dismutase, total hexosamine, and prostaglandin synthesis. Amongst the five fractions from the ethanolic extract of P. minus, the ethyl acetate:methanol 1:1 v/v fraction (F2) significantly (p < 0.005) exhibited better inhibition of ulcer lesions in a dose-dependent manner. In addition, rats pre-treated with F2 showed a significant elevation in superoxide dismutase (SOD), hexosamine and PGE2 levels in the stomach wall mucosa in a dose-dependent matter. Based on these results, the ethyl acetate:methanol 1:1 v/v fraction was considered to be the best fraction for mucous protection in the ethanol induction model. The mechanisms underlying this protection were attributed to the synthesis of antioxidants and PGE2

Adenovirus-Mediated Gene Transduction of Pancreatic Tumour Cells Lines

Pancreatic cancer is often a fatal disease due to its poor response to existing therapies. Thus new therapeutic approaches must be developed. The aim of this study was to evaluate the susceptibility of pancreatic cell lines to adenovirus-mediated transduction and to determine the expression of surface coxsackie B and adenovirus receptor (CAR). Five human pancreatic cancer cell lines were transduced using a recombinant adenovirus type 5 expressing enhanced green fluorescent protein (Ad5EGFP) and the expression of EGFP was analysed by flow cytometry. The cell lines varied in transduction efficiency, ranging from less than 1% to more than 30% cells expressing EGFP and susceptibility of cell lines to Ad5EGFP transduction has a positive correlation with the level of surface CAR expression and its presence may be a limiting step for efficient adenovirus transduction. These results suggest that gene therapy of pancreatic tumours is feasible, but then the status of CAR expression in the tumour needs to be evaluated