Fatty-acid uptake in prostate cancer cells using dynamic microfluidic raman technology

It is known that intake of dietary fatty acid (FA) is strongly correlated with prostate cancer progression but is highly dependent on the type of FAs. High levels of palmitic acid (PA) or arachidonic acid (AA) can stimulate the progression of cancer. In this study, a unique experimental set-up consisting of a Raman microscope, coupled with a commercial shear-flow microfluidic system is used to monitor fatty acid uptake by prostate cancer (PC-3) cells in real-time at the single cell level. Uptake of deuterated PA, deuterated AA, and the omega-3 fatty acids docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA) were monitored using this new system, while complementary flow cytometry experiments using Nile red staining, were also conducted for the validation of the cellular lipid uptake. Using this novel experimental system, we show that DHA and EPA have inhibitory effects on the uptake of PA and AA by PC-3 cells

Numerical Modelling of Instantaneous Plate Tectonics

Assuming lithospheric plates to be rigid, we systematically invert 68 spreading rates, 62 fracture zones trends and 10^6 earthquake slip vectors simultaneously to obtain a self-consistent model of instantaneous relative motions for eleven major plates. The inverse problem is linearized and solved iteratively by a maximum likelihood procedure. Because the uncertainties in the data are small, Gaussian statistics are shown to be adequate. The use of a linear theory permits (1) the calculation of the uncertainties in the various angular velocity vectors caused by uncertainties in the data, and (2) quantitative examination of the distribution of information within the data set. The existence of a self-consistent model satisfying all the data is strong justification of the rigid plate assumption. Slow movement between North and South America is shown to be resolvable. We then invert the trends of 20 linear island chains and aseismic ridges under the assumptions that they represent the directions of plate motions over a set of hot spots fixed with respect to each other. We conclude that these hot spots have had no significant relative motions in the last 10 My

Serving GODAE Data and Products to the Ocean Community

The Global Ocean Data Assimilation Experiment (GODAE [http:// www.godae.org]) has spanned a decade of rapid technological development. The ever-increasing volume and diversity of oceanographic data produced by in situ instruments, remote-sensing platforms, and computer simulations have driven the development of a number of innovative technologies that are essential for connecting scientists with the data that they need. This paper gives an overview of the technologies that have been developed and applied in the course of GODAE, which now provide users of oceanographic data with the capability to discover, evaluate, visualize, download, and analyze data from all over the world. The key to this capability is the ability to reduce the inherent complexity of oceanographic data by providing a consistent, harmonized view of the various data products. The challenges of data serving have been addressed over the last 10 years through the cooperative skills and energies of many individuals

Knot homology via derived categories of coherent sheaves II, sl(m) case

Using derived categories of equivariant coherent sheaves we construct a knot homology theory which categorifies the quantum sl(m) knot polynomial. Our knot homology naturally satisfies the categorified MOY relations and is conjecturally isomorphic to Khovanov-Rozansky homology. Our construction is motivated by the geometric Satake correspondence and is related to Manolescu's by homological mirror symmetry.Comment: 51 pages, 9 figure

LoCuSS: The steady decline and slow quenching of star formation in cluster galaxies over the last four billion years

We present an analysis of the levels and evolution of star formation activity in a representative sample of 30 massive galaxy clusters at 0.15<z<0.30 from the Local Cluster Substructure Survey (LoCuSS), combining wide-field Spitzer 24um data with extensive spectroscopy of cluster members. The specific-SFRs of massive (M>10^10 M_sun) star-forming cluster galaxies within r200 are found to be systematically 28% lower than their counterparts in the field at fixed stellar mass and redshift, a difference significant at the 8.7-sigma level. This is the unambiguous signature of star formation in most (and possibly all) massive star-forming galaxies being slowly quenched upon accretion into massive clusters, their SFRs declining exponentially on quenching time-scales in the range 0.7-2.0 Gyr. We measure the mid-infrared Butcher-Oemler effect over the redshift range 0.0-0.4, finding rapid evolution in the fraction (f_SF) of massive (M_K3M_sun/yr, of the form f_SF (1+z)^7.6. We dissect the origins of the Butcher-Oemler effect, revealing it to be due to the combination of a ~3x decline in the mean specific-SFRs of star-forming cluster galaxies since z~0.3 with a ~1.5x decrease in number density. Two-thirds of this reduction in the specific-SFRs of star-forming cluster galaxies is due to the steady cosmic decline in the specific-SFRs among those field galaxies accreted into the clusters. The remaining one-third reflects an accelerated decline in the star formation activity of galaxies within clusters. The slow quenching of star-formation in cluster galaxies is consistent with a gradual shut down of star formation in infalling spiral galaxies as they interact with the intra-cluster medium via ram-pressure stripping or starvation mechanisms. We find no evidence for the build-up of cluster S0 bulges via major nuclear star-burst episodes.Comment: 24 pages, 12 figures. Accepted for publication in Ap

Star formation in the massive cluster merger Abell 2744

We present a comprehensive study of star-forming (SF) galaxies in the HST Frontier Field recent cluster merger A2744 (z=0.308). Wide-field, ultraviolet-infrared (UV-IR) imaging enables a direct constraint of the total star formation rate (SFR) for 53 cluster galaxies, with SFR{UV+IR}=343+/-10 Msun/yr. Within the central 4 arcmin (1.1 Mpc) radius, the integrated SFR is complete, yielding a total SFR{UV+IR}=201+/-9 Msun/yr. Focussing on obscured star formation, this core region exhibits a total SFR{IR}=138+/-8 Msun/yr, a mass-normalised SFR{IR} of Sigma{SFR}=11.2+/-0.7 Msun/yr per 10^14 Msun and a fraction of IR-detected SF galaxies f{SF}=0.080(+0.010,-0.037). Overall, the cluster population at z~0.3 exhibits significant intrinsic scatter in IR properties (total SFR{IR}, Tdust distribution) apparently unrelated to the dynamical state: A2744 is noticeably different to the merging Bullet cluster, but similar to several relaxed clusters. However, in A2744 we identify a trail of SF sources including jellyfish galaxies with substantial unobscured SF due to extreme stripping (SFR{UV}/SFR{IR} up to 3.3). The orientation of the trail, and of material stripped from constituent galaxies, indicates that the passing shock front of the cluster merger was the trigger. Constraints on star formation from both IR and UV are crucial for understanding galaxy evolution within the densest environments.Comment: Accepted by MNRAS. 12 pages, 7 figures (high resolution versions of Figs. 1 & 2 are available in the published PDF

Cosmic dance in the Shapley Concentration Core - I. A study of the radio emission of the BCGs and tailed radio galaxies

The Shapley Concentration ($z\approx0.048$) covers several degrees in the Southern Hemisphere, and includes galaxy clusters in advanced evolutionary stage, groups of clusters in the early stages of merger, fairly massive clusters with ongoing accretion activity, and smaller groups located in filaments in the regions between the main clusters. With the goal to investigate the role of cluster mergers and accretion on the radio galaxy population, we performed a multi-wavelength study of the BCGs and of the galaxies showing extended radio emission in the cluster complexes of Abell 3528 and Abell 3558. Our study is based on a sample of 12 galaxies. We observed the clusters with the GMRT at 235, 325 and 610 MHz, and with the VLA at 8.46 GHz. We complemented our study with the TGSS at 150 MHz, the SUMSS at 843 MHz and ATCA at 1380, 1400, 2380, and 4790 MHz data. Optical imaging with ESO-VST and mid-IR coverage with WISE are also available for the host galaxies. We found deep differences in the properties of the radio emission of the BCGs in the two cluster complexes. The BCGs in the A3528 complex and in A3556, which are relaxed cool-core objects, are powerful active radio galaxies. They also present hints of restarted activity. On the contrary, the BCGs in A3558 and A3562, which are well known merging systems, are very faint, or quiet, in the radio band. The optical and IR properties of the galaxies are fairly similar in the two complexes, showing all passive red galaxies. Our study shows remarkable differences in the radio properties of the BGCs, which we relate to the different dynamical state of the host cluster. On the contrary, the lack of changes between such different environments in the optical band suggests that the dynamical state of galaxy clusters does not affect the optical counterparts of the radio galaxies, at least over the life-time of the radio emission.Comment: 24 pages, 11 figures, accepted for publication in Astronomy & Astrophysic

Tests of Statistical Significance and Background Estimation in Gamma Ray Air Shower Experiments

In this paper we discuss several methods of significance calculation and point out the limits of their applicability. We then introduce a self consistent scheme for source detection and discuss some of its properties. The method allows incorporating background anisotropies by vetoing existing small scale regions on the sky and compensating for known large scale anisotropies. By giving an example using Milagro gamma ray observatory we demonstrate how the method can be employed to relax the detector stability assumption. Two practical implementations of the method are discussed. The method is universal and can be used with any large field-of-view detector, where the object of investigation, steady or transient, point or extended, traverses its field of view.Comment: 12 pages, 7 figures. Submitted to ApJ, submission number: 5833