Getting research findings into practice : when to act on the evidence.

There is increasing interest in providing evidence based health care—that is, care in which healthcare professionals, provider managers, those who commission health care, the public, and policymakers consistently consider research evidence when making decisions. Purchasers, for example, should be able to influence the organisation and delivery of care (such as for cancer and stroke services) and the type and content of services (such as using chiropractic for back pain or dilatation and curettage and drug treatment for menorrhagia). Policymakers should ensure that policies on treatment reflect and are consistent with research evidence, and that the incentive structure within the health system promotes cost effective practice. They must also ensure that there is an adequate infrastructure for monitoring changes in practice and for producing, gathering, summarising, and disseminating evidence. Clinicians determine the day to day care patients receive in healthcare systems, and user groups (for example, patients, their families, and their representatives) are also beginning to play an important role in influencing healthcare decisions

Permeability evolution across carbonate hosted normal fault zones

Acknowledgements: The authors would like to thank Total E&P and BG Group for project funding and support, and the Industry Technology Facilitator for facilitating the collaborative development (grant number 3322PSD). The authors would also like to express their gratitude to the Aberdeen Formation Evaluation Society and the College of Physical Sciences at the University of Aberdeen for partial financial support. Raymi Castilla (Total E&P), Fabrizio Agosta and Cathy Hollis are also thanked for their constructive comments and suggestions to improve the standard of this manuscript as are John Still and Colin Taylor (University of Aberdeen) for technical assistance in the laboratory. Piero Gianolla is thanked for his editorial handling of the manuscript.Peer reviewedPostprin

Peculiar Velocities of Nonlinear Structure: Voids in McVittie Spacetime

As a study of peculiar velocities of nonlinear structure, we analyze the model of a relativistic thin-shell void in the expanding universe. (1) Adopting McVittie (MV) spacetime as a background universe, we investigate the dynamics of an uncompensated void with negative MV mass. Although the motion itself is quite different from that of a compensated void, as shown by Haines & Harris (1993), the present peculiar velocities are not affected by MV mass. (2) We discuss how precisely the formula in the linear perturbation theory applies to nonlinear relativistic voids, using the results in (1) as well as the previous results for the homogeneous background (Sakai, Maeda, & Sato 1993). (3) We re-examine the effect of the cosmic microwave background radiation. Contrary to the results of Pim & Lake (1986, 1988), we find that the effect is negligible. We show that their results are due to inappropriate initial conditions. Our results (1)-(3) suggest that the formula in the linear perturbation theory is approximately valid even for nonlinear voids.Comment: 12 pages, aastex, 4 ps figures separate, Fig.2 added, to appear in Ap

Shapley Supercluster Survey: Ram-Pressure Stripping vs. Tidal Interactions in the Shapley Supercluster

We present two new examples of galaxies undergoing transformation in the Shapley supercluster core. These low-mass (stellar mass from 0.4E10 to 1E10 Msun) galaxies are members of the two clusters SC-1329-313 (z=0.045) and SC-1327-312 (z=0.049). Integral-field spectroscopy complemented by imaging in ugriK bands and in Halpha narrow-band are used to disentangle the effects of tidal interaction (TI) and ram-pressure stripping (RPS). In both galaxies, SOS-61086 and SOS-90630, we observe one-sided extraplanar ionized gas extending respectively 30kpc and 41kpc in projection from their disks. The galaxies' gaseous disks are truncated and the kinematics of the stellar and gas components are decoupled, supporting the RPS scenario. The emission of the ionized gas extends in the direction of a possible companion for both galaxies suggesting a TI. The overall gas velocity field of SOS-61086 is reproduced by ad hoc N-body/hydrodynamical simulations of RPS acting almost face-on and starting about 250Myr ago, consistent with the age of the young stellar populations. A link between the observed gas stripping and the cluster-cluster interaction experienced by SC-1329-313 and A3562 is suggested. Simulations of ram pressure acting almost edge-on are able to fully reproduce the gas velocity field of SOS-90630, but cannot at the same time reproduce the extended tail of outflowing gas. This suggests that an additional disturbance from a TI is required. This study adds a piece of evidence that RPS may take place in different environments with different impacts and witnesses the possible effect of cluster-cluster merger on RPS.Comment: 27 pages, 28 figures, MNRAS accepte

Dissecting Trait Heterogeneity: a Comparison of Three Clustering Methods Applied to Genotypic Data

Background: Trait heterogeneity, which exists when a trait has been defined with insufficient specificity such that it is actually two or more distinct traits, has been implicated as a confounding factor in traditional statistical genetics of complex hu man disease. In the absence of de tailed phenotypic data collected consistently in combination with genetic data, unsupervised computational methodologies offer the potential for discovering underlying trait heteroge neity. The performance of three such methods – Bayesian Classification, Hyperg raph-Based Clustering, and Fuzzy k -Modes Clustering – appropriate for categorical data were comp ared. Also tested was the ability of these methods to detect trait heterogeneity in the presence of locus heteroge neity and/or gene-gene interaction , which are two other complicating factors in discovering genetic models of complex human disease. To dete rmine the efficacy of applying the Bayesian Classification method to re al data, the reliability of its intern al clustering metr ics at finding good clusterings was evaluated using permutation testing. Results: Bayesian Classifica tion outperformed the other two method s, with the exception that the Fuzzy k -Modes Clustering performed best on the most comp lex genetic model. Bayesian Classificati on achieved excellent recovery for 75% of the da tasets simulated under the simplest genetic model, while it achieved moderate recovery for 56% of datase ts with a sample size of 500 or more (across all simulated models) and for 86% of datasets with 10 or fewer nonfuncti onal loci (across all si mulated models). Neither Hypergraph Clustering nor Fuzzy k -Modes Clustering achieved good or excellent cluster recovery for a majority of datasets even under a re stricted set of conditions. When usin g the average log of class strength as the internal clustering metric, th e false positive rate was controlled very well, at three percent or less for all three significance levels (0. 01, 0.05, 0.10), and the false negative rate was acceptably low (18 percent) for the least stringent sign ificance level of 0.10. Conclusion: Bayesian Classificati on shows promise as an unsuper vised computational method for dissecting trait hetero geneity in genotypic data. Its control of fa lse positive and false negative rates lends confidence to the validity of its results. Further investigation of how differ ent parameter settings may improve the performance of Bayesian Classification, especi ally under more comp lex genetic models, is ongoing

Shapley Supercluster Survey (ShaSS): Galaxy Evolution from Filaments to Cluster Cores

We present an overview of a multi-wavelength survey of the Shapley supercluster (SSC; z~0.05) covering a contiguous area of 260 h^-2_70 Mpc^2 including the supercluster core. The project main aim is to quantify the influence of cluster-scale mass assembly on galaxy evolution in one of the most massive structures in the local Universe. The Shapley supercluster survey (ShaSS) includes nine Abell clusters (A3552, A3554, A3556, A3558, A3559, A3560, A3562, AS0724, AS0726) and two poor clusters (SC1327- 312, SC1329-313) showing evidence of cluster-cluster interactions. Optical (ugri) and near-infrared (K) imaging acquired with VST and VISTA allow us to study the galaxy population down to m*+6 at the supercluster redshift. A dedicated spectroscopic survey with AAOmega on the Anglo-Australian Telescope provides a magnitude-limited sample of supercluster members with 80% completeness at ~m*+3. We derive the galaxy density across the whole area, demonstrating that all structures within this area are embedded in a single network of clusters, groups and filaments. The stellar mass density in the core of the SSC is always higher than 9E09 M_sun Mpc^-3, which is ~40x the cosmic stellar mass density for galaxies in the local Universe. We find a new filamentary structure (~7 Mpc long in projection) connecting the SSC core to the cluster A3559, as well as previously unidentified density peaks. We perform a weak-lensing analysis of the central 1 sqdeg field of the survey obtaining for the central cluster A3558 a mass of M_500=7.63E14 M_sun, in agreement with X-ray based estimates.Comment: 22 pages, 11 figures. Accepted for publication on MNRA

Squalane is in the midplane of the lipid bilayer: implications for its function as a proton permeability barrier

AbstractA recently proposed model for proton leakage across biological membranes [Prog. Lipid Res. 40 (2001) 299] suggested that hydrocarbons specifically in the center of the lipid bilayer inhibit proton leaks. Since cellular membranes maintain a proton electrochemical gradient as a principal energy transducer, proton leakage unproductively consumes cellular energy. Hydrocarbons in the bilayer are widespread in membranes that sustain such gradients. The alkaliphiles are unique in that they contain up to 40 mol% isoprenes in their membranes including 10–11 mol% squalene [J. Bacteriol. 168 (1986) 334]. Squalene is a polyisoprene hydrocarbon without polar groups. Localizing hydrocarbons in lipid bilayers has not been trivial. A myriad of physical methods including fluorescence spectroscopy, electron-spin resonance, nuclear magnetic resonance as well as X-ray and neutron diffraction have been used to explore this question with various degrees of success and often contradictory results. Seeking unambiguous evidence for the localization of squalene in membranes or lipid bilayers, we employed neutron diffraction. We incorporated 10 mol% perdeuterated or protonated squalane, an isosteric analogue of squalene, into stacked bilayers of dioleoyl phosphatidyl choline (DOPC) doped with dioleoyl phosphatidyl glycerol (DOPG) to simulate the negative charges found on natural membranes. The neutron diffraction data clearly show that the squalane lies predominantly in the bilayer center, parallel to the plane of the membrane

Social influences on microglial reactivity and neuronal damage after cardiac arrest/cardiopulmonary resuscitation

Social isolation presents a risk factor and worsens outcome to cerebrovascular diseases; however, the underlying mechanisms remain underspecified. This study examines the effect of social environment on microglial reactivity after global cerebral ischemia, to test the hypothesis that social isolation leads to greater microglial responses. Adult female and male mice were pair-housed or socially isolated for one week prior to cardiac arrest/cardiopulmonary resuscitation (CA/CPR) or the sham procedure, and following either 2 or 24 hours of reperfusion, microglia samples were enriched and analyzed for gene expression. At the 2-hour time point, microglia from both females and males exhibited ischemia-induced inflammation, characterized by the gene expression increase of tumor necrosis factor alpha (TNF-α), interleukin 1 beta (IL-1β) and interleukin 6 (IL-6), regardless of the housing conditions. However, at 24 hours post-ischemia, social housing attenuated microglial pro-inflammatory gene expression in a sex-specific manner. At this time point, the ischemia-induced increased expression of IL-1β and IL-6 was attenuated by social interaction in microglia from male mice, while among female mice social attenuation of the inflammatory response was observed in the microglial expression of cell surface protein major histocompatibility complex II (MHC II). A second study examined behavioral and physiological measures 96 hours after ischemic injury. At this time point, female and male mice displayed increased locomotion and exploratory behavior following CA/CPR relative to controls. Regardless of sex, ischemia also elicited neuroinflammation and neurodegeneration, both of which were modulated by the social environment. Hippocampal nitric oxide (iNOS), cortical TNF-α, and counts of Fluoro-Jade C positive stained cells in the CA1 region of the hippocampus, were increased in the isolated CA/CPR group relative to sham controls and the pair-housed CA/CPR groups. Together, these data indicate that female and male mice exhibit similar outcome measures and social modulation at 96 hours post-ischemic injury, nonetheless, that social environment influences microglial reactivity to global cerebral ischemia in a sex-specific manner

Hydrogen atom moving across a strong magnetic field: analytical approximations

Analytical approximations are constructed for binding energies, quantum-mechanical sizes and oscillator strengths of main radiative transitions of hydrogen atoms arbitrarily moving in magnetic fields 10^{12}-10^{13} G. Examples of using the obtained approximations for determination of maximum transverse velocity of an atom and for evaluation of absorption spectra in magnetic neutron star atmospheres are presented.Comment: 17 pages, 3 figures, 5 tables, LaTeX with IOP style files (included). In v.2, Fig.1 and Table 5 have been corrected. In v.3, a misprint in the fit for oscillator strengths, Eq.(21), has been correcte