3,769 research outputs found

    Processed Chili Peppers for Export Markets: A Capital Budgeting Study on the AgroFood Company

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    The AgroFood Company, which currently exports fresh chili peppers to European clients, desires to expand the product mix offered. The company, as it expands its production of fresh peppers for export, has an increasing supply of grade 2 peppers that are unmarketable in Egypt. However, an attractive market for processed frozen chili peppers exists in Europe. To expand their client base, capitalize on a value added product, and minimize product waste, the AgroFood Company desires to develop processing practices for chili peppers produced in Egypt. The AgroFood Company would like to identify its options in the processed pepper market. An analysis of the company, competition, consumer, market channel, and conditions, provides insight into possible solutions to the challenges faced by the farm management. Designed for undergraduate classroom use, this case will provide students with an opportunity to evaluate the merits of business expansion into a high capacity, automated mechanical processing facility for grade 2 vegetables.Decision case, horticulture, agriculture economics, chili pepper production, protected vegetable production, Crop Production/Industries, Food Security and Poverty, Production Economics,

    A Practical and Efficient Synthesis of Uniform Conjugated Rod‐Like Oligomers

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    Herein, a more practical and efficient synthesis protocol for the preparation of uniform rod-like oligo(1,4-phenylene ethynylene)s (OPE)s is presented. Applying an iterative reaction cycle consisting of a decarboxylative coupling reaction and a saponification of an alkynyl carboxylic ester, a uniform pentamer is obtained in ten steps with 14% overall yield. The copper-free conditions prevent homocoupling until the trimer stage, resulting in a significantly easier work-up of the products. Homocoupling is observed from the tetramer stage on, but a simple variation of the work-up procedure also yields the uniform tetramer and pentamer. A thorough comparison with the commonly used and described Sonogashira approach reveals that with the new presented strategy, OPEs can be built in similar overall yield, but easier purification and in a quarter of the time. All oligomers are fully characterized by proton and carbon nuclear magnetic resonance spectroscopy (NMR), mass spectrometry (MS), size-exclusion chromatography (SEC), and infrared spectroscopy (IR)

    Gene discovery using massively parallel pyrosequencing to develop ESTs for the flesh fly Sarcophaga crassipalpis

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    <p>Abstract</p> <p>Background</p> <p>Flesh flies in the genus <it>Sarcophaga </it>are important models for investigating endocrinology, diapause, cold hardiness, reproduction, and immunity. Despite the prominence of <it>Sarcophaga </it>flesh flies as models for insect physiology and biochemistry, and in forensic studies, little genomic or transcriptomic data are available for members of this genus. We used massively parallel pyrosequencing on the Roche 454-FLX platform to produce a substantial EST dataset for the flesh fly <it>Sarcophaga crassipalpis</it>. To maximize sequence diversity, we pooled RNA extracted from whole bodies of all life stages and normalized the cDNA pool after reverse transcription.</p> <p>Results</p> <p>We obtained 207,110 ESTs with an average read length of 241 bp. These reads assembled into 20,995 contigs and 31,056 singletons. Using BLAST searches of the NR and NT databases we were able to identify 11,757 unique gene elements (E<0.0001) representing approximately 9,000 independent transcripts. Comparison of the distribution of <it>S. crassipalpis </it>unigenes among GO Biological Process functional groups with that of the <it>Drosophila melanogaster </it>transcriptome suggests that our ESTs are broadly representative of the flesh fly transcriptome. Insertion and deletion errors in 454 sequencing present a serious hurdle to comparative transcriptome analysis. Aided by a new approach to correcting for these errors, we performed a comparative analysis of genetic divergence across GO categories among <it>S. crassipalpis</it>, <it>D. melanogaster</it>, and <it>Anopheles gambiae</it>. The results suggest that non-synonymous substitutions occur at similar rates across categories, although genes related to response to stimuli may evolve slightly faster. In addition, we identified over 500 potential microsatellite loci and more than 12,000 SNPs among our ESTs.</p> <p>Conclusion</p> <p>Our data provides the first large-scale EST-project for flesh flies, a much-needed resource for exploring this model species. In addition, we identified a large number of potential microsatellite and SNP markers that could be used in population and systematic studies of <it>S. crassipalpis </it>and other flesh flies.</p

    Research Notes : United States : Genes for resistance to Phytophthora megasperma f. sp. glycinea in PI 273483D, PI 64747, PI 274212, PI 82312N, and PI 340046

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    Several years ago, we identified seven plant introductions resistant to the 16 races of Phytophthora megasperma f. sp. glycinea Kuan and Erwin (Pmg) known at that time. Each of these was crossed to the eight cultivars in Table 1 to determine how resistance was controlled. They were not crossed to cultivars that contained Rps2 or Rps5 because Rps2 was found using root inoculation in a liquid culture solution, and Rps5 was described after this study was started

    Dietary and body mass thresholds for reproduction in grasshoppers

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    An organism’s dietary protein should match its respective dietary needs to yield the most advantageous effects; an extended lifespan and increased reproductive output. The key challenge however, is how to tailor a specific diet to an organism’s individual needs. Applying the technique of Piper et. al, we can approximate the optimal diet of the lubber grasshopper, Romalea microptera, by using the AA profile of vitellogenin (Vg), the precursor to egg yolk protein. The lubber grasshopper, Romalea microptera, was selected because of its plasticity in reproductive responsiveness in response to diet quality and quantity. Each of the organisms were fed 1g of romaine lettuce, ad libitum (ad-lib) zero protein high carbohydrate artificial diet and a different experimentally manipulated diet. The 4 treatment groups, Vg-balanced AA, unbalanced AA, ad-lib lettuce, and dietary restriction (DR), dietary treatments were applied twice daily from day 2 of adulthood to egg laying. The experimental group was force-fed the balanced AA diet, which was derived from the AA composition of vitellogenin. The unbalanced AA group was fed an isonitrogenous diet with over representations of AA’s found in romaine. The ad lib group had unlimited access to romaine and was force-fed PBS. The DR group was fed a diet comprising of 1 gram of romaine and PBS. The results indicated ad-lib group had the highest yield, followed by Vg-balanced, unbalanced, and DR . In contrast, somatic mass and storage did not differ across 3 groups fed 1gm lettuce daily. Isonitrogenous diets exhibited difference in reproduction but not somatic growth

    Dielectronic recombination of xenonlike tungsten ions

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    Dielectronic recombination (DR) of xenonlike W20+ forming W19+ has been studied experimentally at a heavy-ion storage ring. A merged-beams method has been employed for obtaining absolute rate coefficients for electron-ion recombination in the collision-energy range 0–140 eV. The measured rate coefficient is dominated by strong DR resonances even at the lowest experimental energies. At plasma temperatures where the fractional abundance of W20+ is expected to peak in a fusion plasma, the experimentally derived plasma recombination rate coefficient is over a factor of 4 larger than the theoretically calculated rate coefficient which is currently used in fusion plasma modeling. The largest part of this discrepancy stems most probably from the neglect in the theoretical calculations of DR associated with fine-structure excitations of the W20+([Kr]4d10 4f8) ion core
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