Monitoring volatile aroma compounds during fermentation in a chemically defined grape juice medium deficient in Leucine

Model media are frequently used to study wine fermentation outcomes, as they are considered an acceptable and reproducible representation of real juices. Identifying and quantifying the progression of the aroma compounds formed during fermentation is of importance for studies aimed at tailoring winemaking outcomes. Overexpression libraries of the yeast Saccharomyces cerevisiae can be used to determine the impact of the overexpression of specific genes. Recently, an S. cerevisiae overexpression library has been reported that encompasses a plasmid-borne construct, which utilizes a leucine-selectable marker to differentiate between yeast that contain the plasmid and those that do not. As such, these experiments with this library require a leucine-deficient fermentation medium. Aroma-progression studies have been performed in real juice and in an MS300 model medium; however, to date, none have been performed in chemically defined grape juice medium (CDGJM) or in media lacking certain amino acids. This study reports on the progression of a library of 34 enologically relevant aroma compounds formed during fermentation using a leucine-requiring wine-strain derivative of S. cerevisiae bearing the overexpression library platform plasmid and grown in a CDGJM-Leu medium. The results indicate that the production and accumulation of all 34 aroma compounds followed similar progression trends to those found in previous studies with MS300 and juice-exploiting wild-type yeast, with the exception of the compounds associated with pathways connected to the biosynthesis and metabolism of leucine. The findings confirm the likely utility of this system for evaluating the importance of overexpression of specific genes in aroma-compound production.</p

Energetic Particles and Acceleration Regions Over Jupiter's Polar Cap and Main Aurora: A Broad Overview

Previous Juno mission event studies revealed powerful electron and ion acceleration, to 100s of kiloelectron volts and higher, at low altitudes over Jupiter's main aurora and polar cap (PC; poleward of the main aurora). Here we examine 30–1200 keV JEDI-instrument particle data from the first 16 Juno orbits to determine how common, persistent, repeatable, and ordered these processes are. For the PC regions, we find (1) upward electron angle beams, sometimes extending to megaelectron volt energies, are persistently present in essentially all portions of the polar cap but are generated by two distinct and spatially separable processes. (2) Particle evidence for megavolt downward electrostatic potentials are observable for 80 of the polar cap crossings and over substantial fractions of the PC area. For the main aurora, with the orbit favoring the duskside, we find that (1) three distinct zones are observed that are generally arranged from lower to higher latitudes but sometimes mixed. They are designated here as the diffuse aurora (DifA), Zone-I (ZI(D)) showing primarily downward electron acceleration, and Zone-II (ZII(B)) showing bidirectional acceleration with the upward intensities often greater than downward intensities. (2) ZI(D) and ZII(B) sometimes (but not always) contain, respectively, downward electron inverted Vs and downward proton inverted Vs, (potentials up to 400 kV) but, otherwise, have broadband distributions. (3) Surprisingly, both ZI(D) and ZII(B) can generate equally powerful auroral emissions. It is suggested but demonstrated for intense portions of only one auroral crossing, that ZI(D) and ZII(B) are associated, respectively, with upward and downward electric currents

The Gut Mucosal Viral Reservoir in HIV-Infected Patients Is Not the Major Source of Rebound Plasma Viremia following Interruption of Highly Active Antiretroviral Therapy ▿ †

Interruption of suppressive highly active antiretroviral therapy (HAART) in HIV-infected patients leads to increased HIV replication and viral rebound in peripheral blood. Effects of therapy interruption on gut-associated lymphoid tissue (GALT) have not been well investigated. We evaluated longitudinal changes in viral replication and emergence of viral variants in the context of T cell homeostasis and gene expression in GALT of three HIV-positive patients who initiated HAART during primary HIV infection but opted to interrupt therapy thereafter. Longitudinal viral sequence analysis revealed that a stable proviral reservoir was established in GALT during primary HIV infection that persisted through early HAART and post-therapy interruption. Proviral variants in GALT and peripheral blood mononuclear cells (PBMCs) displayed low levels of genomic diversity at all times. A rapid increase in viral loads with a modest decline of CD4+ T cells in peripheral blood was observed, while gut mucosal CD4+ T cell loss was severe following HAART interruption. This was accompanied by increased mucosal gene expression regulating interferon (IFN)-mediated antiviral responses and immune activation, a profile similar to those found in HAART-naive HIV-infected patients. Sequence analysis of rebound virus suggested that GALT was not the major contributor to the postinterruption plasma viremia nor were GALT HIV reservoirs rapidly replaced by HIV rebound variants. Our data suggest an early establishment and persistence of viral reservoirs in GALT with minimal diversity. Early detection of and therapy for HIV infection may be beneficial in controlling viral evolution and limiting establishment of diverse viral reservoirs in the mucosal compartment

Sedimentary reservoir oxidation during geologic CO2 sequestration

Injection of carbon dioxide into subsurface geologic reservoirs during geologic carbon sequestration (GCS) introduces an oxidizing supercritical CO2 phase into a subsurface geologic environment that is typically reducing. The resulting redox disequilibrium provides the chemical potential for the reduction of CO2 to lower free energy organic species. However, redox reactions involving carbon typically require the presence of a catalyst. Iron oxide minerals, including magnetite, are known to catalyze oxidation and reduction reactions of C-bearing species. If the redox conditions in the reservoir are modified by redox transformations involving CO2, such changes could also affect mineral stability, leading to dissolution and precipitation reactions and alteration of the long-term fate of CO2 in GCS reservoirs. We present experimental evidence that reservoirs with reducing redox conditions are favorable environments for the relatively rapid abiotic reduction of CO2 to organic molecules. In these experiments, an aqueous suspension of magnetite nanoparticles was reacted with supercritical CO2 under pressure and temperature conditions relevant to GCS in sedimentary reservoirs (95-210°C and ~100bars of CO2). Hydrogen production was observed in several experiments, likely caused by Fe(II) oxidation either at the surface of magnetite or in the aqueous phase. Heating of the Fe(II)-rich system resulted in elevated PH2 and conditions favorable for the reduction of CO2 to acetic acid. Implications of these results for the long-term fate of CO2 in field-scale systems were explored using reaction path modeling of CO2 injection into reservoirs containing Fe(II)-bearing primary silicate minerals, with kinetic parameters for CO2 reduction obtained experimentally. The results of these calculations suggest that the reaction of CO2 with reservoir constituents will occur in two primary stages (1) equilibration of CO2 with organic acids resulting in mineral-fluid disequilibrium, and (2) gradual dissolution of primary minerals promoting significant CO2 reduction through the release of Fe(II). The reduction of CO2 is identified as a new trapping mechanism that could significantly enhance the long-term stability of GCS reservoirs. Identification of reservoir characteristics that promote CO2 redox transformations could be used as an additional factor in screening geologic reservoirs for GCS