El CORVIS en cirugía refractiva

Durant les últimes dècades, la cirurgia refractiva s’ha tornat un mètode molt utilitzat per a corregir un determinat defecte refractiu. La cirurgia refractiva es pot dividir en dos grups, segons l’anatomia en la qual es realitzi la correcció. Existeixen tècniques corneals i altres tècniques que es refereixen a la implantació de lents intraoculars (cirurgia intraocular) amb la finalitat d’aconseguir la emetropía. Davant la resposta corneal, s’ha estudiat àmpliament la biomecánica corneal. Es tracta d’una ciencia que estudia l’equilibri i la deformació del teixit corneal en ser sotmès a qualsevol força. Se’l considera de gran rellevància per a la cirurgia ja que té el potencial de prevenir futures ectasies post-cirurgía refractiva. En l’actualitat es disposa d’instruments que permeten un mesurament clínic de la biomeànica corneal i capacitat de caracteritzar si la còrnia és apta per a la cirurgia corneal. En aquest treball s’ha estudiat uns dels ells, que es CORVIS ST. El Corvis ST és un tonometre de no contacte, capaç d’avaluar in vivo els paràmetres corneals relacionats amb el comportament biomecànic de la cornea. També calcula la PIO i la paquimetria corneal, oferint resultats fiables. L’estudi bibliográfic que s’ha realizat en aquest Treball Final de Grau mostra l’ús clínic de Corvis ST en cirurgia refractiva.Durante las últimas décadas, la cirugía refractiva se ha vuelto un método muy utilizado para corregir un determinado defecto refractivo (miopía, hipermetropía y astigmatismo). La cirugía refractiva se puede dividir en dos grupos, según la anatomía en la que se realice la corrección. Existen técnicas corneales y otras técnicas que se refieren a la implantación de lentes intraoculares (cirugía intraocular) con el fin de conseguir la emetropía. Frente a la respuesta corneal, se ha estudiado ampliamente la biomecánica corneal. Se trata de una ciencia que estudia el equilibrio y la deformación del tejido corneal al ser sometido a cualquier fuerza. Se le considera de gran relevancia para la cirugía ya que tiene el potencial de prevenir futuras ectasias post-cirugía refractiva. En la actualidad se dispone de instrumentos que permiten una medición clínica de la biomecánica corneal y capacidades de caracterizar si la córnea es apta para la cirugía corneal. En este trabajo se ha estudiado a una de ellos, que es CORVIS ST. El Corvis ST es un tonómetro de no contacto, capaz de evaluar in vivo los parámetros corneales relacionados con el comportamiento biomecánico de la córnea. También calcula la PIO y la paquimetría corneal, ofreciendo resultados fiables. El estudio bibliográfico que se ha realizado en este Trabajo Final de Grado muestra el uso clínico de Corvis ST en cirugía refractiva.During the past decades, refractive surgery has become a widely used method to correct a determined refractive defect (myopia, hyperopia, and astigmatism). Refractive surgery is divided into two groups, according to the anatomy in which the correction is performed. There are corneal procedures and other techniques that are referred to as the implantation of intraocular lenses (intraocular surgery) in order to achieve emmetropia. In the context of corneal response, corneal biomechanics has been extensively studied. It is a science that examines the equilibrium and deformation of corneal tissue when being subjected to any force. It is regarded as being of great relevance to surgery as it has the capability of varying the results. Currently, there are instruments that allow clinical measurement of corneal biomechanics and the ability to characterize whether the cornea is appropriate for corneal surgery. In this review, we have studied one of such instruments, which is CORVIS ST. The Corvis St is a non-contact tonometer, capable of evaluating in vivo corneal parameters related to the biomechanical behavior of the cornea. It also calculates IOP and corneal pachymetry, providing reliable results. The literature study conducted in this Final Degree Project shows the clinical use of Corvis ST in refractive surgery

Transvaginal Ultrasonographic Findings of Infertile Females in Population of Lahore

Failure of conception in women after frequent unprotected intercourse for one year is known as infertility. Ultrasound examination can detect certain pathologies that may result in primary or secondary infertility. Objective: To describe the various transvaginal ultrasonographic findings of infertile females in population of Lahore. Methods: The study was started after the consent of ethical committee of the University of Lahore. All the patients were registered in this study after signing the informed consent form. Toshiba Xario with transvaginal transducer frequency ranging from 9-12 MHz was used. Pathologies were evaluated through transvaginal scanning and sonographic data was kept in the ultrasound machine. A consecutive sampling technique was used and data was further evaluated with the help of Statistical Package for the Social Sciences version 24. Results: Among 138 females, PCOS was seen in 40.6% of the subjects. The second most common pathology was ovarian cyst which was seen in 10.9% of subjects. And the incidence of multiple fibroids was 5.1% as third most common pathology in both primary and secondary infertility cases. Most ovarian pathologies were found to be bilateral. 12.3% subjects had no findings on ultrasound. Conclusion: PCOS was the utmost common pathology connected with primary and secondary infertility. The second and third most common pathologies were ovarian cyst and multiple fibroids respectively. Keywords: female infertility, PCOS, PID DOI: 10.7176/JBAH/10-12-05 Publication date:June 30th 202

Ecology and Genetic Identification of Freshwater Turtles in Pakistan

Background: The turtle population plays an important role in sustaining the water ecosystem by minimizing pollution from water. The identification and molecular investigation of freshwater fauna is essential for conservation of the species that are near to extinction. The quality of water, type of flora, fauna, and environmental condition are the major factors that directly affect the distribution of freshwater turtles. Studies on the species diversity and habitat of freshwater turtle have not been focused previously in the region. The present study was the first conducted to estimate the habitat and genetic diversity of freshwater turtles using 12S rRNA (ribosomal RNA) gene in Pakistan.Materials, Methods & Results: A total of 26 samples were collected from various localities using hand net, cast net, gills net, steel hooks, thick chemical wire, using chicken intestine and small fishes. The collected turtle specimens were morpho-taxonomically categorized into two genera, Lissemys punctata andersoni (n=13, 50%) and Nilssonia gangetica (n=13, 50%). The collected species showed an aggressive and active behavior in captivity during summer. Genomic DNA was extracted from collected specimens and used in PCR reaction by using specific primers for the amplification of short fragments of 12S rRNA gene. Analysis of generated sequences confirmed the existence of L. p. andersoni in the region. The generated sequences of L. p. andersoni correspond to Clad A and showed a close resemblance among different species of the genus Lissemys.Discussion: This study is the first investigation about the habitat and of the endemic turtle species L. p. andersoni and N. gangetica in Pakistan. The genetic identification followed by phylogenetic analysis based on 12S rRNA partial genes revealed a closest similarity with the sequences generated for the same species from the neighboring countries. This study provided information to conduct further molecular studies that are essential to provide significant genetic data about turtle species.Keywords: turtle, ecology, diversity, phylogeny, Pakistan

Ecology and genetic identification of freshwater turtles in Pakistan

Background: The turtle population plays an important role in sustaining the water ecosystem by minimizing pollution from water. The identification and molecular investigation of freshwater fauna is essential for conservation of the species that are near to extinction. The quality of water, type of flora, fauna, and environmental condition are the major factors that directly affect the distribution of freshwater turtles. Two families including eight species of freshwater turtles are found in Pakistan. The Geoemydidae (Geoclemys hamiltonii, Hardella thurjii, Pangshura smithii, and Pangshura tecta) and Trionychidae (Chitra indica, Nilssonia gangetica, Nilssonia hurum, and Lissemys punctata andersoni). Studies on the species diversity and habitat of freshwater turtle have not been focused previously in the region. The present study was the first conducted to estimate the habitat and genetic diversity of freshwater turtles using 12S rRNA (ribosomal RNA) gene in Pakistan. Materials, Methods & Results: A total of 26 samples were collected from various localities using hand net, cast net, gills net, steel hooks, thick chemical wire, using chicken intestine and small fishes. The collected turtle specimens were morpho-taxonomically categorized into two genera, Lissemys punctata andersoni (n=13, 50%) and Nilssonia gangetica (n=13, 50%). The collected species showed an aggressive and active behavior in captivity during summer. Genomic DNA was extracted from collected specimens and used in PCR reaction by using specific primers for the amplification of short fragments of 12S rRNA gene. Analysis of generated sequences confirmed the existence of L. p. andersoni in the region. The generated sequences of L. p. andersoni correspond to Clad A and showed a close resemblance among different species of the genus Lissemys. Discussion: The climatic change such as temperature and rainfall have great effects on the occurrence of turtles. Habitat degradation occurred due to various factors such as draining wetlands, deforestation, converting clear water rivers to stagnant multi-purpose reservoirs and mortality on roads when turtles move around to feed. Current study concluded that the freshwater turtles L. p. andersoni and N. gangetica are interested in natural feeds. The analysis of 359 bp of 12S rRNA gene of the genus Lissemys turtles showed relationships of these turtles with cyclanorbines flap shell turtles, which agrees with previous reports. The African taxa are paraphyletic with respect to the Asian Lissemys. The ancestors of the extant genus cyclanorbines spread from North America to Asia [26]. It should be expected that each of the 3 taxa, L. p. andersoni, L. p. punctata and L. scutata represents a distinct genetic lineage. Present molecular investigation concluded that Clad A comprising L. p. punctata, L. scutata, L. cylonensis also include L. p. andersoni species. Clad B also contains one sequence from India, identified as L. p. andersoni. Their classification as conspecific evolutionary lineages are suggested by similar genetic divergences, the observation of mismatches between morphology (spotted vs. unspotted) and mitochondrial haplotypes in clades A and B. The clades A and B provides evidence for gene flow between the spotted subspecies L. p. andersoni and adjacent populations with unspotted flap shell turtles. This study is the first investigation about the habitat and of the endemic turtle species L. p. andersoni and N. gangetica in Pakistan. The genetic identification followed by phylogenetic analysis based on 12S rRNA partial genes revealed a closest similarity with the sequences generated for the same species from the neighboring countries. This study provided information to conduct further molecular studies that are essential to provide significant genetic data about turtle species

Frequency of Different Types of Focal Hepatic Lesions on Triphasic Computed Tomography Scan in Adult Patients

Out of various pathologies that affect the liver, focal liver lesion form an important group. Triphasic computed tomography is the imaging modality most often used to evaluate focal liver lesions. The purpose of this study was to evaluate the role of computed tomography in the diagnosis of liver lesions and to determine frequency of these lesions.Keywords: Liver, focal lesion, liver parenchyma, enhancement, iso-dense, hypo-dense and hyper-denseDOI: 10.7176/JHMN/77-04Publication date:July 31st 202

Enhancement of Extender Excellence of Frozen Bull Semen Using α-Tocopherol as an Antioxidant

Background: Addition of the antioxidant to extender media is the most appropriate attempt to reduce structural losses encounter during the process of cryopreservation. Hence semen excellence could be maintained for longer duration without adverse impact. Additionally antioxidants are not only capturing the reactive oxygen species but also improve the sperm quality indicators and fertility. Accordingly, current elucidation has been executed to explore the dose depended appraisal of varied concentration of α-tocopherol in Tris-based extender on frozen-thawed bull semen quality parameters for enhancement of bull semen cryopreservation in the subtropical ecosystem of Peshawar.Materials, Methods & Results: Experiments were carried out on semen that has been collected from both Achai-an indigenous breed and Holstein Friesian (HF) - the exotic breed in artificial vagina maintained at 42°C from the experimental bulls of either breed and processed independently breed wise. Semen specimens with above 70% motility were evaluated separately breed-wise under same environmental condition. Standard procedure was adopted to extend the collected semen in the experimental extenders and frozen subsequently. After thawing, further Analysis of the frozen straws of semen was carried out for sperm excellence indicators that include motility, viability, acrosomal integrity and functional integrity of spermatozoa under the subtropical condition. Sperm viability and acrosomal integrity were determined by dual staining procedure i.e. Trypan-blue and Giemsa stains. The hypo-osmotic swelling (HOS) test was used to assess plasma membrane integrity. The current elucidation demonstrated that α-tocopherol 1.5 mg/mL supplemented in extender had significantly (P < 0.05) increased sperm excellence gauge that includes motility, viability, acrosomal integrity and functional membrane integrity in both the breeds. On the other hand, the result further elucidated those concentrations higher or lower than 1.5-1 mg/mL α-tocopherol supplemented in the present study resulted in a lower semen functional attributes subsequent to freezing.Discussion: Biological samples were preserved by various methods such cryopreservation at low temperature to maintain them intact and facilitate downstream experiments. Antioxidants were associated with the chemical breakage of the substrate consequential from oxidation and counterbalance the free radicals thus diminish the damaging impacts to spermatozoa in cryopreservation. There is lack of data with respect to lipid peroxidation and cell reinforcement limit in cryopreserved semen, and cryopreservation is related with the generation of receptive oxygen species (ROS) which lead to lipid peroxidation (LPO) of sperm membranes, bringing about lost motility, viability and fertility of sperm. Recently, in the reproductive management of the dairy animals, an important focus of research is to overcome the deleterious impact associated with semen cryopreservation. Elucidation of differential dose depended expression of α-tocopherol in semen extender has been evident in this study. The results were corroborated with diverse mammalian species under varied ecological management setups. Furthermore the detrimental impact of high doses of α-tocopherol in the semen extender has been demonstrated. It was deduced from the current finding that addition of α-tocopherol via dose depended approach in Tri extender enhanced the cryosurvivability of both Achai indigenous breed and HF the exotic breed, under existing reproductive management in the local ecosystem of Peshawar

Odd skipped-related 1 controls the pro-regenerative response of fibro-adipogenic progenitors

Skeletal muscle regeneration requires the coordinated interplay of diverse tissue-resident- and infiltrating cells. Fibro-adipogenic progenitors (FAPs) are an interstitial cell population that provides a beneficial microenvironment for muscle stem cells (MuSCs) during muscle regeneration. Here we show that the transcription factor Osr1 is essential for FAPs to communicate with MuSCs and infiltrating macrophages, thus coordinating muscle regeneration. Conditional inactivation of Osr1 impaired muscle regeneration with reduced myofiber growth and formation of excessive fibrotic tissue with reduced stiffness. Osr1-deficient FAPs acquired a fibrogenic identity with altered matrix secretion and cytokine expression resulting in impaired MuSC viability, expansion and differentiation. Immune cell profiling suggested a novel role for Osr1-FAPs in macrophage polarization. In vitro analysis suggested that increased TGFβ signaling and altered matrix deposition by Osr1-deficient FAPs actively suppressed regenerative myogenesis. In conclusion, we show that Osr1 is central to FAP function orchestrating key regenerative events such as inflammation, matrix secretion and myogenesis