Analytical, experimental and numerical study of a graded honeycomb structure under in-plane impact load with low velocity

Given the significance of energy absorption in various industries, light shock absorbers such as honeycomb structure under in-plane and out-of-plane loads have been in the core of attention. The purpose of this research is the analyses of graded honeycomb structure (GHS) behaviour under in-plane impact loading and its optimisation. Primarily, analytical equations for plateau stress and specific energy are represented, taking power hardening model (PHM) and elastic–perfectly plastic model (EPPM) into consideration. For the validation and comparison of acquired analytical equations, the energy absorption of a GHS made of five different aluminium grades is simulated in ABAQUS/CAE. In order to validate the numerical simulation method in ABAQUS, an experimental test has been conducted as the falling a weight with low velocity on a GHS. Numerical results retain an acceptable accordance with experimental ones with a 5.4% occurred error of reaction force. For a structure with a specific kinetic energy, the stress–strain diagram is achieved and compared with the analytical equations obtained. The maximum difference between the numerical and analytical plateau stresses for PHM is 10.58%. However, this value has been measured to be 38.78% for EPPM. In addition, the numerical value of absorbed energy is compared to that of analytical method for two material models. The maximum difference between the numerical and analytical absorbed energies for PHM model is 6.4%, while it retains the value of 48.08% for EPPM. Based on the conducted comparisons, the numerical and analytical results based on PHM are more congruent than EPPM results. Applying sequential quadratic programming method and genetic algorithm, the ratio of structure mass to the absorbed energy is minimised. According to the optimisation results, the structure capacity of absorbing energy increases by 18% compared to the primary model

Transcriptional Responses of Resistant and Susceptible Fish Clones to the Bacterial Pathogen Flavobacterium psychrophilum

Flavobacterium psychrophilum is a bacterial species that represents one of the most important pathogens for aquaculture worldwide, especially for salmonids. To gain insights into the genetic basis of the natural resistance to F. psychrophilum, we selected homozygous clones of rainbow trout with contrasted susceptibility to the infection. We compared the transcriptional response to the bacteria in the pronephros of a susceptible and a resistant line by micro-array analysis five days after infection. While the basal transcriptome of healthy fish was significantly different in the resistant and susceptible lines, the transcriptome modifications induced by the bacteria involved essentially the same genes and pathways. The response to F. psychrophilum involved antimicrobial peptides, complement, and a number of enzymes and chemokines. The matrix metalloproteases mmp9 and mmp13 were among the most highly induced genes in both genetic backgrounds. Key genes of both pro- and anti-inflammatory response such as IL1 and IL10, were up-regulated with a greater magnitude in susceptible animals where the bacterial load was also much higher. While higher resistance to F. psychrophilum does not seem to be based on extensive differences in the orientation of the immune response, several genes including complement C3 showed stronger induction in the resistant fish. They may be important for the variation of susceptibility to the infection

Estimates of linkage disequilibrium and effective population size in rainbow trout

<p>Abstract</p> <p>Background</p> <p>The use of molecular genetic technologies for broodstock management and selective breeding of aquaculture species is becoming increasingly more common with the continued development of genome tools and reagents. Several laboratories have produced genetic maps for rainbow trout to aid in the identification of loci affecting phenotypes of interest. These maps have resulted in the identification of many quantitative/qualitative trait loci affecting phenotypic variation in traits associated with albinism, disease resistance, temperature tolerance, sex determination, embryonic development rate, spawning date, condition factor and growth. Unfortunately, the elucidation of the precise allelic variation and/or genes underlying phenotypic diversity has yet to be achieved in this species having low marker densities and lacking a whole genome reference sequence. Experimental designs which integrate segregation analyses with linkage disequilibrium (LD) approaches facilitate the discovery of genes affecting important traits. To date the extent of LD has been characterized for humans and several agriculturally important livestock species but not for rainbow trout.</p> <p>Results</p> <p>We observed that the level of LD between syntenic loci decayed rapidly at distances greater than 2 cM which is similar to observations of LD in other agriculturally important species including cattle, sheep, pigs and chickens. However, in some cases significant LD was also observed up to 50 cM. Our estimate of effective population size based on genome wide estimates of LD for the NCCCWA broodstock population was 145, indicating that this population will respond well to high selection intensity. However, the range of effective population size based on individual chromosomes was 75.51 - 203.35, possibly indicating that suites of genes on each chromosome are disproportionately under selection pressures.</p> <p>Conclusions</p> <p>Our results indicate that large numbers of markers, more than are currently available for this species, will be required to enable the use of genome-wide integrated mapping approaches aimed at identifying genes of interest in rainbow trout.</p