Dynamic concentration of motors in microtubule arrays

We present experimental and theoretical studies of the dynamics of molecular motors in microtubule arrays and asters. By solving a convection-diffusion equation we find that the density profile of motors in a two-dimensional aster is characterized by continuously varying exponents. Simulations are used to verify the assumptions of the continuum model. We observe the concentration profiles of kinesin moving in quasi two-dimensional artificial asters by fluorescent microscopy and compare with our theoretical results.Comment: 4pages, 4 figures revte

The homotopy theory of simplicial props

The category of (colored) props is an enhancement of the category of colored operads, and thus of the category of small categories. In this paper, the second in a series on "higher props," we show that the category of all small colored simplicial props admits a cofibrantly generated model category structure. With this model structure, the forgetful functor from props to operads is a right Quillen functor.Comment: Final version, to appear in Israel J. Mat

Validity of Critical Velocity Concept for Weighted Sprinting Performance

International Journal of Exercise Science 11(4): 900-909, 2018. We investigated the validity of a recently developed equation for predicting sprinting times of various tactical loads based upon the performance of a running 3-min all-out exercise test (3MT). Thirteen recreationally trained participants completed the running 3MT to determine critical velocity (CV) and finite running capacity for running velocities exceeding CV (D’). Two subsequent counterbalanced loaded sprints of 800 and 1000 m distances with 20 and 15% of their body mass, respectively, were evaluated. Estimated times (t, sec) for running 800 and 1000 m with a tactical load was derived using t = (D – D’)/CV. Critical velocity adjusted for an added load using the following regression equation: original CV + (-0.0638 x %load) + 0.6982, D was 800 or 1000 m, and whole percentage load was ~15 or 20% of the participant\u27s body mass. From the 3MT, CV (3.80 ±0.5 m.s-1) and D’(200 ±49.88 m) values were determined.The typical error of predicting actual times for the 800 and 1000 m loaded sprints were 5.6 and 10.1 s, with corresponding ICCs of 0.95 and 0.87, and coefficient of variations of 2.9 and 4.3%. The effect size differences between estimated and actual sprint times were small (0.27) and moderate (0.60) for 800 and 1000 m, respectively. The adjustment to CV through the regression equation yields small to moderate overestimates of maximally loaded sprint times for distances of 800 and 1000 m. Whether such errors remain pervasive for prescribing high-intensity interval training is unclear and requires further investigation

The Effect of Static Stretching, Mini-Band Warm-Ups, Medicine-Ball Warm-Ups, and a Light Jogging Warm-Up on Common Athletic Ability Tests

International Journal of Exercise Science 13(4): 298-311, 2020. Proper warm-up is important for facilitating peak athletic performance and reducing injury risk; yet, warm-up procedures vary considerably amongst coaches and athletes. The purpose of this study was to assess the effect of a static stretching, medicine-ball, and mini-band warm-ups relative to a light jogging warm-up only on athletic ability test performance. It was hypothesized that static stretching would negatively affect performance, while medicine-ball and mini-band warm-ups would positively affect performance relative to light jogging only. Twelve female collegiate soccer players (19.3 ± 1.2y, 65.2 ± 7.5kg, 1.67 ± 0.07m) participated in this study. Athletes completed each warm-up protocol and all of the athletic performance tests over four sessions in a semi-randomized, counterbalanced order. An omnibus MANOVA with vertical jump height, medicine ball throw distance, 10m and 20m sprint time, and T-test time as the dependent variables was not significant indicating that warm up did not have an effect on subsequent athletic ability test performance [Wilks’ λ = 0.64, F(15,110) = 1.28, p= 0.23, η2= 0.14]. Static stretching warm-up did not negatively influence athletic potential compared to mini-band and medicine ball warm-ups, though the most optimal warm-up is likely athlete specific

Effects of Intensive Training on Prolactin Responses to Submaximal Exercise in Males

The purpose of this study was to determine if serum prolactin responses to submaximal exercise were affected by 8 weeks of intensive training (5 cl/wk, 90 min/d 65-200% V02max). Nine males performed 90 minute continuous exercise bouts (cycle ergometry; 65% V02 max) at the end of 1, 4, and 8 weeks of training. Blood samples were obtained pre-training, and pre-, post-exercise. Significant differences were not seen in pre- and post-exercise prolactin levels at weeks 1 and 4. However, at week 8 the post-exercise prolatin was significantly greater than the pre-exercise levels (6.8 ± 0.9 vs 3.8 ± 1.0 ng·ml-1; P-1, respectively). The findings suggest intensive training results in a relative augmentation of the post-exercise prolactin response; however, this effect seems to be due primarily to the training induced lowered resting prolactin levels

Mutations in protocadherin 15 and cadherin 23 affect tip links and mechanotransduction in mammalian sensory hair cells

Immunocytochemical studies have shown that protocadherin-15 (PCDH15) and cadherin-23 (CDH23) are associated with tip links, structures thought to gate the mechanotransducer channels of hair cells in the sensory epithelia of the inner ear. The present report describes functional and structural analyses of hair cells from Pcdh15av3J (av3J), Pcdh15av6J (av6J) and Cdh23v2J (v2J) mice. The av3J and v2J mice carry point mutations that are predicted to introduce premature stop codons in the transcripts for Pcdh15 and Cdh23, respectively, and av6J mice have an in-frame deletion predicted to remove most of the 9th cadherin ectodomain from PCDH15. Severe disruption of hair-bundle morphology is observed throughout the early-postnatal cochlea in av3J/av3J and v2J/v2J mice. In contrast, only mild-to-moderate bundle disruption is evident in the av6J/av6J mice. Hair cells from av3J/av3J mice are unaffected by aminoglycosides and fail to load with [3H]-gentamicin or FM1-43, compounds that permeate the hair cell's mechanotransducer channels. In contrast, hair cells from av6J/av6J mice load with both FM1-43 and [3H]-gentamicin, and are aminoglycoside sensitive. Transducer currents can be recorded from hair cells of all three mutants but are reduced in amplitude in all mutants and have abnormal directional sensitivity in the av3J/av3J and v2J/v2J mutants. Scanning electron microscopy of early postnatal cochlear hair cells reveals tip-link like links in av6J/av6J mice, substantially reduced numbers of links in the av3J/av3J mice and virtually none in the v2J/v2J mice. Analysis of mature vestibular hair bundles reveals an absence of tip links in the av3J/av3J and v2J/v2J mice and a reduction in av6J/av6J mice. These results therefore provide genetic evidence consistent with PCDH15 and CDH23 being part of the tip-link complex and necessary for normal mechanotransduction

Muscle Adaptations Following Short-Duration Bed Rest with Integrated Resistance, Interval, and Aerobic Exercise

Unloading of the musculoskeletal system during space flight results in deconditioning that may impair mission-related task performance in astronauts. Exercise countermeasures have been frequently tested during bed rest (BR) and limb suspension; however, high-intensity, short-duration exercise prescriptions have not been fully explored. PURPOSE: To determine if a high intensity resistance, interval, and aerobic exercise program could protect against muscle atrophy and dysfunction when performed during short duration BR. METHODS: Nine subjects (1 female, 8 male) performed a combination of supine exercises during 2 weeks of horizontal BR. Resistance exercise (3 d / wk) consisted of squat, leg press, hamstring curl, and heel raise exercises (3 sets, 12 repetitions). Aerobic (6 d / wk) sessions alternated continuous (75% VO2 peak) and interval exercise (30 s, 2 min, and 4 min) and were completed on a supine cycle ergometer and vertical treadmill, respectively. Muscle volumes of the upper leg were calculated pre, mid, and post-BR using magnetic resonance imaging. Maximal isometric force (MIF), rate of force development (RFD), and peak power of the lower body extensors were measured twice before BR (averaged to represent pre) and once post BR. ANOVA with repeated measures and a priori planned contrasts were used to test for differences. RESULTS: There were no changes to quadriceps, hamstring, and adductor muscle volumes at mid and post BR time points compared to pre BR (Table 1). Peak power increased significantly from 1614 +/- 372 W to 1739 +/- 359 W post BR (+7.7%, p = 0.035). Neither MIF (pre: 1676 +/- 320 N vs. post: 1711 +/- 250 N, +2.1%, p = 0.333) nor RFD (pre: 7534 +/- 1265 N/ms vs. post: 6951 +/- 1241 N/ms, -7.7%, p = 0.136) were significantly impaired post BR