13 research outputs found
Human PKR Transfected into Murine Cells Stimulates Expression of Genes under Control of the HIV1 or HTLV-I LTR
AbstractWe have analyzed the effect of transfection into murine NIH/3T3 cells of the human dsRNA-activated kinase PKR on the expression of the β-galactosidase reporter gene, placed under control of the HIV1 or the HTLV-I LTR. β-Galactosidase expression is stimulated when the reporter plasmids are cotransfected with wild-type PKR but inhibited when cotransfected with a catalytically inactive mutant PKR. In the case of HIV1, β-galactosidase expression was not stimulated when cotransfection was carried out with PKR harboring mutations in the dsRNA binding domains, indicating that stimulation depends on the classical mode of PKR activation through dsRNA binding. In contrast, the dsRNA binding mutants of PKR could still partially stimulate β-galactosidase expression from the HTLV-I LTR, suggesting that PKR activation in this case may involve different/additional mechanisms. These results show that, in addition to the known down-regulation of protein synthesis through eIF2 phosphorylation, PKR can also positively stimulate gene expressionin vivo,most probably through phosphorylation of a substrate distinct from eIF2
Assay and the levels of 2-5A-synthetase in lymphocytes of patients with viral, bacterial and autoimmune diseases
Human PKR Transfected into Murine Cells Stimulates Expression of Genes under Control of the HIV1 or HTLV-1 LTR
Efficient synthesis and comparative studies of the arginine and N(omega),N(omega)-dimethylarginine forms of the human nucleolin glycine/arginine rich domain.
The anti-HIV pseudopeptide HB-19 binds the c-terminal end of nucleolin and prevents enchorage of virus particles on target cells.
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