321 research outputs found
Factors that affect proliferation of Salmonella in tomatoes post-harvest: the roles of seasonal effects, irrigation regime, crop and pathogen genotype
MAIN OBJECTIVES: Fresh fruits and vegetables become increasingly recognized as vehicles of human salmonellosis. Physiological, ecological, and environmental factors are all thought to contribute to the ability of Salmonella to colonize fruits and vegetables pre- and post-harvest. The goal of this study was to test how irrigation levels, fruit water congestion, crop and pathogen genotypes affect the ability of Salmonella to multiply in tomatoes post-harvest. EXPERIMENTAL DESIGN: Fruits from three tomato varieties, grown over three production seasons in two Florida locations, were infected with seven strains of Salmonella and their ability to multiply post-harvest in field-grown tomatoes was tested. The field experiments were set up as a two-factor factorial split plot experiment, with the whole-plot treatments arranged in a randomized complete-block design. The irrigation treatment (at three levels) was the whole-plot factor, and the split-plot factor was tomato variety, with three levels. The significance of the main, two-way, and three-way interaction effects was tested using the (type III) F-tests for fixed effects. Mean separation for each significant fixed effect in the model was performed using Tukey's multiple comparison testing procedure. MOST IMPORTANT DISCOVERIES AND SIGNIFICANCE: The irrigation regime per se did not affect susceptibility of the crop to post-harvest proliferation of Salmonella. However, Salmonella grew significantly better in water-congested tissues of green tomatoes. Tomato maturity and genotype, Salmonella genotype, and inter-seasonal differences were the strongest factors affecting proliferation. Red ripe tomatoes were significantly and consistently more conducive to proliferation of Salmonella. Tomatoes harvested in the driest, sunniest season were the most conducive to post-harvest proliferation of the pathogen. Statistically significant interactions between production conditions affected post-harvest susceptibility of the crop to the pathogen. UV irradiation of tomatoes post-harvest promoted Salmonella growth
Effects of nitrogen and potassium fertilization on the susceptibility of tomatoes to post-harvest proliferation of Salmonella enterica
Fresh fruits and vegetables are increasingly recognized as vehicles of salmonellosis. Pre- and post-harvest environmental conditions, and physiological, and genetic factors are thought to contribute to the ability of human pathogens to persist in the production environment, attach to, colonize and proliferate in and on raw produce. How field production conditions affect the post-harvest food safety outcomes is not entirely understood. This study tested how varying nitrogen and potassium fertilization levels affected the "susceptibility" of tomatoes to Salmonella infections following the harvest of fruits. Two tomato varieties grown over three seasons under high, medium, and low levels of nitrogen and potassium fertilization in two locations were inoculated with seven strains of Salmonella. Even though the main effects of nitrogen and potassium fertilization on the susceptibility of tomatoes to infections with Salmonella enterica were not statistically significant overall, differences in nitrogen concentrations in plant tissues correlated with the susceptibility of partially ripe tomatoes (cv. Solar Fire) to Salmonella. Tomato maturity and the season in which tomatoes were produced had the strongest effect on the ability of Salmonella to multiply in tomatoes. Tomato phenolics, accumulation of which is known to correlate with rates of the N fertilization, did not inhibit growth of Salmonella in vitro
Ethylene signalling affects susceptibility of tomatoes to Salmonella
Fresh fruits and vegetables are increasingly recognized as important reservoirs of human pathogens, and therefore, significant attention has been directed recently to understanding mechanisms of the interactions between plants and enterics, like Salmonella. A screen of tomato cultivars for their susceptibility to Salmonella revealed significant differences in the ability of this human pathogen to multiply within fruits; expression of the Salmonella genes (cysB, agfB, fadH) involved in the interactions with tomatoes depended on the tomato genotype and maturity stage. Proliferation of Salmonella was strongly reduced in the tomato mutants with defects in ethylene synthesis, perception and signal transduction. While mutation in the ripening-related ethylene receptor Nr resulted only in a modest reduction in Salmonella numbers within tomatoes, strong inhibition of the Salmonella proliferation was observed in rin and nor tomato mutants. RIN and NOR are regulators of ethylene synthesis and ripening. A commercial tomato variety heterozygous for rin was less susceptible to Salmonella under the greenhouse conditions but not when tested in the field over three production seasons
Imaging the Earth's Interior: the Angular Distribution of Terrestrial Neutrinos
Decays of radionuclides throughout the Earth's interior produce geothermal
heat, but also are a source of antineutrinos. The (angle-integrated)
geoneutrino flux places an integral constraint on the terrestrial radionuclide
distribution. In this paper, we calculate the angular distribution of
geoneutrinos, which opens a window on the differential radionuclide
distribution. We develop the general formalism for the neutrino angular
distribution, and we present the inverse transformation which recovers the
terrestrial radioisotope distribution given a measurement of the neutrino
angular distribution. Thus, geoneutrinos not only allow a means to image the
Earth's interior, but offering a direct measure of the radioactive Earth, both
(1) revealing the Earth's inner structure as probed by radionuclides, and (2)
allowing for a complete determination of the radioactive heat generation as a
function of radius. We present the geoneutrino angular distribution for the
favored Earth model which has been used to calculate geoneutrino flux. In this
model the neutrino generation is dominated by decays in the Earth's mantle and
crust; this leads to a very ``peripheral'' angular distribution, in which 2/3
of the neutrinos come from angles > 60 degrees away from the downward vertical.
We note the possibility of that the Earth's core contains potassium; different
geophysical predictions lead to strongly varying, and hence distinguishable,
central intensities (< 30 degrees from the downward vertical). Other
uncertainties in the models, and prospects for observation of the geoneutrino
angular distribution, are briefly discussed. We conclude by urging the
development and construction of antineutrino experiments with angular
sensitivity. (Abstract abridged.)Comment: 25 pages, RevTeX, 7 figures. Comments welcom
Mimicking diffuse supernova antineutrinos with the Sun as a source
Measuring the electron antineutrino component of the cosmic diffuse supernova
neutrino background (DSNB) is the next ambitious goal for low-energy neutrino
astronomy. The largest flux is expected in the lowest accessible energy bin.
However, for E < 15 MeV a possible signal can be mimicked by a solar electron
antineutrino flux that originates from the usual 8B neutrinos by spin-flavor
oscillations. We show that such an interpretation is possible within the
allowed range of neutrino electromagnetic transition moments and solar
turbulent field strengths and distributions. Therefore, an unambiguous
detection of the DSNB requires a significant number of events at E > 15 MeV.Comment: 4 pages, 1 figur
Stem cell differentiation increases membrane-actin adhesion regulating cell blebability, migration and mechanics
This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder in order to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/K. S. is funded by an EPSRC PhD studentship. S.T. is funded by an EU Marie Curie Intra European Fellowship (GENOMICDIFF)
The see-saw mechanism: neutrino mixing, leptogenesis and lepton flavor violation
The see-saw mechanism to generate small neutrino masses is reviewed. After
summarizing our current knowledge about the low energy neutrino mass matrix we
consider reconstructing the see-saw mechanism. Low energy neutrino physics is
not sufficient to reconstruct see-saw, a feature which we refer to as ``see-saw
degeneracy''. Indirect tests of see-saw are leptogenesis and lepton flavor
violation in supersymmetric scenarios, which together with neutrino mass and
mixing define the framework of see-saw phenomenology. Several examples are
given, both phenomenological and GUT-related. Variants of the see-saw mechanism
like the type II or triplet see-saw are also discussed. In particular, we
compare many general aspects regarding the dependence of LFV on low energy
neutrino parameters in the extreme cases of a dominating conventional see-saw
term or a dominating triplet term. For instance, the absence of mu -> e gamma
or tau -> e gamma in the pure triplet case means that CP is conserved in
neutrino oscillations. Scanning models, we also find that among the decays mu
-> e gamma, tau -> e gamma and tau -> mu gamma the latter one has the largest
branching ratio in (i) SO(10) type I see-saw models and in (ii) scenarios in
which the triplet term dominates in the neutrino mass matrix.Comment: 26 pages, 7 figures. Expanded version of talk given at 10th Workshop
In High Energy Physics Phenomenology (WHEPP 10), January 2008, Chennai,
India. Typos corrected, comments and references adde
The Influence of Supplemental Zinc and Ractopamine Hydrochloride on the Performance and Longissimus Thoracis Proteome of Finishing Beef Steers
To determine how Zn and ractopamine hydrochloride (RAC) supplementation affect the longissimus thoracis muscle proteome in beef cattle, 48 high percentage Angus steers (494±18.2 kg) were utilized in a 2×2 factorial study design. Steers were blocked by body weight (BW) and genetic gain potential (GeneMax; Zoetis, Parsippany, NJ) into control (CON; analyzed 36 mg Zn/kg dry matter [DM]) or supranutritional Zn (SUPZN; CON+60 mg Zn/kg DM from ZnSO4+60 mg Zn/kg DM from Zn-amino acid complex; Availa Zn, Zinpro, Eden Prairie, MN) dietary treatments (ZNTRT). Starting 28 d prior to harvest, steers were blocked by BW within ZNTRT to RAC treatments (RACTRT) of 0 (NO) or 300 mg·steer−1·d−1 (RAC; Actogain 45, Zoetis). After 15 d on RACTRT, longissimus thoracis biopsies were collected from all steers for proteomic analysis. Twenty-eight steers (n=7 per treatment) were harvested at a commercial abattoir on Day 90. A ZNTRT×RACTRT effect showed SUPZN-RAC steers had a greater dressing percentage than other treatments (P≤0.02). Steers fed SUPZN had a greater hot carcass weight, carcass-adjusted final BW, overall average daily gain, and overall gain to feed (P≤0.05). Differentially abundant proteins involved in energy metabolism, muscle structure, and protein synthesis potentially indicate muscle fiber characteristic differences because of Zn and RAC supplementation.Both myosin light chain kinase 2 and eukaryotic translation initiation factor 5A-1 were more abundant in SUPZN-RAC steers than SUPZN-NO (P≤0.05) or CON-NO (P≤0.04) steers when analyzed for phosphorylation modifications. L-lactate dehydrogenase B was more abundant in SUPZN-NO steers than CON-NO (P=0.03) or CON-RAC (P=0.01) steers. In conclusion, increased Zn supplementation may be needed to optimize the hypertrophic effects of RAC through its effects on the longissimus thoracis proteome
Repressing Anarchy in Neutrino Mass Textures
The recent results that is relatively large, of the order of
the previous upper bound, and the indications of a sizable deviation of
from the maximal value are in agreement with the predictions of
Anarchy in the lepton sector. The quark and charged lepton hierarchies can then
be reproduced in a SU(5) GUT context by attributing non-vanishing
charges, different for each family, only to the SU(5) tenplet states. The fact
that the observed mass hierarchies are stronger for up quarks than for down
quarks and charged leptons supports this idea. As discussed in the past, in the
flexible context of , different patterns of charges can
be adopted going from Anarchy to various types of hierarchy. We revisit this
approach by also considering new models and we compare all versions to the
present data. As a result we confirm that, by relaxing the extreme ansatz of
equal charges for all SU(5) pentaplets and singlets, better
agreement with the data than for Anarchy is obtained without increasing the
model complexity. We also present the distributions obtained in the different
models for the Dirac CP-violating phase. Finally we discuss the relative merits
of these simple models.Comment: v1: 12 pages, 3 figures; v2: 13 pages, 3 figures, text improved,
matches version accepted for publication; v3: submitted to add an
acknowledgment to a networ
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