Pengaruh Ekstrak Metanol Akar Pasak Bumi (Eurycoma Longifolia, Jack.) terhadap Jumlah Splenosit Mencit Diinfeksi Listeria monocytogenes

Percobaan ini dirancang untuk mempelajari pengaruh ekstrak metanol akar E. longifolia, Jack (MEEL) pada respon imun spesifik dengan penekanan khusus pada jumlah splenoctyes. Desain penelitian adalah posttest only control group design. Empat puluh delapan tikus betina Balb / c yang digunakan di seluruh. Empat puluh delapan tikus yang digunakan berpikir studi, dan dibagi menjadi 4 kelompok. Kelompok pertama sebagai kontrol hanya menerima aquadest. Kelompok II, III, dan IV diberikan 100, 200 , dan 400 mg / kgBB per hari, masing-masing yang MEEL diberikan bersama 14 hari. Kemudian, pada hari-15, diinfeksi Listeria monocytogenes. Observasi dilakukan pada hari 0, 3, dan 10 setelah infeksi. Jumlah splenocytes layak diukur dengan MTT Assay. Data dianalisis menggunakan Anova satu jalan. Hasil penelitian menunjukkan bahwa ekstrak metanol E. longifolia, Jack pada 200 mg / kg BB per hari sejauh 14 hari dapat meningkatkan persentase splenocytes, dan puncaknya terdeteksi pada hari-0 infeksi, pada Kii 11.363% yaitu, pada hari-3 setelah infeksi di KIII, yaitu 10.433% dan pada hari-10 di KIII yaitu 8.907%. Uji Anova menunjukkan perbedaan bermakna dengan pengecualian kelompok I dengan IV, dan II dengan III dan III dengan IV. Disimpulan ekstrak metanol E. longifolia, Jack. root mampu meningkatkan persentase splenocytes di Balb / c tikus yang terinfeksi dengan L. monocytogenes

Expression of MRP8/MRP14mRNAin Monocytes of Periodontitis: Comparison between Diabetic and Non Diabetic Patients

The severity of periodontitis on patients with type 2 Diabetes Mellitus patients was strongly thought caused by decreasing of leukocytes function such as monoctyes and neutrophils. In our previous research it was found that calprotectin (MRP8/MRP14) level in leukocytes of periodontitis patients with type 2 DMwas higher than periodontitis in non DM.The aim of this study was to determine calprotectin (MRP8/MRP14) mRNAexpression in human monocytes of periodontitis patients with type 2DMand without DM.Monocytes were isolated from the peripheral blood of periodontitis patients with uncontrolled type 2 DM,controlled type 2 DM,and non DM.The expression of total RNAcalprotectin (MRP8and MRP14)were detected by RTPCRusing GAPDHas the innate control. It was observed that the value of MRP8/MRP14mRNAexpression DMpatients were higher than non DM,and the highly significant increase expression (

Expression of MRP8/MRP14 mRNA in Monocytes of Periodontitis: Comparison between Diabetic and Non Diabetic Patients

The severity of periodontitis on patients with type 2 Diabetes Mellitus patients was strongly thought caused by decreasing of leukocytes function such as monoctyes and neutrophils. In our previous research it was found that calprotectin (MRP8/MRP14) level in leukocytes of periodontitis patients with type 2 DM was higher than periodontitis in non DM. The aim of this study was to determine calprotectin (MRP8/MRP14)mRNA expression in human monocytes of periodontitis patients with type 2 DM and without DM. Monocytes were isolated from the peripheral blood of periodontitis patients with uncontrolled type 2 DM, controlled type 2 DM, and non DM. The expression of total RNA calprotectin (MRP8 and MRP14) were detected by RTPCR using GAPDH as the innate control. It was observed that the value of MRP8/MRP14 mRNA expression DM patients were higher than non DM, and the highly significant increase expression (p<0.05) was on the uncontrolled type 2 DM. The basal level of MRP8/MRP14 expression increased in monocyte of periodontitis and type 2 DM patients compared with non diabetes subjects. It was suggested that high basal level MRP8/MRP14 has role in the regulation of severity periodontitis with diabetes mellitus

DAMPAK INFEKSI Porphyromonas gingivalis PADA JARINGAN PERIODONTAL MATERNAL TERHADAP PERTUMBUHAN JANIN

The growth and survival of fetus depends on placenta. Intrauterine infection can result the increased apoptosis of trophoblast during pregnancy. Porphyromonas gingivalis-infected periodontal maternal is expected to result in pregnancy adverse. The aim of this study was to evaluate the effects of Porphyromonas gingivalis infection in periodontal tissues maternal to fetal growth by analyzing the effect of TLR-2, TLR-4, TNF-α, IL-10 and caspase-3 expression in the placenta to the placental weight, fetal weight and fetal length. The subjects of study were Sprague-Dawley female rats, which were subdivided into four groups. Each group consisted of five pregnant rats which euthanized on GD 14 and GD 20. Induction of experimental periodontitis was performed by injection of 0.05 ml live-Porphyromonas gingivalis ATCC 33277 with a concentration of 2x10 9 CFU/ml into the distopalatal and distobuccal gingival sulcus area of maxillary first molar. Blood of the maternal retro-orbital venous plexus and the umbilical cord were conducted immediately to bacteremia test, while the maternal blood serum and amniotic fluid were conducted to endotoxemia test. Each fetus was taken post-mortem from the chorioamniotic sac. Placental weight, fetal weight and fetal length were counted and recorded for each of the maternal. Furthermore, the placental immunohistochemically was undertaken to determine the expression of TLR-2, TLR-4, TNF-α, IL-10 and caspase-3. This study has identified Porphyromonas gingivalis dissemination into the maternal bloodstream and the umbilical cord. Furthermore, the mean of LPS concentrations in amniotic fluid on GD 14 and GD 20 was significantly different (P<0,005) among the four maternal periodontal infection groups. Porphyromonas gingivalis infection in maternal periodontal tissue may result in placental weight, fetal weight and fetal length on the Pg-BD, Pg-B and Pg-D groups lower (P<0,005) than control group on both GD 14 and GD 20. Expression of TLR-4, TLR-2, TNF-α, IL-10 and caspase-3 in the placenta affects (P<0.005) to the placental weight, and the fetal weight and length. The increased expression of TLR-4, TLR-2, TNF-α, IL-10 and caspase-3 in the placenta would result in decreased placental weight, fetal weight and fetal length. The decreased weight of placenta would result in reduced weight and length of fetuses on GD 14 and GD 20. Therefore, it can be concluded that the maternal periodontal health during pregnancy affects maternal status implied by changes in placental function and fetal growth. Porphyromonas gingivalis infection in maternal periodontal tissue can result in decreased placental weight, fetal weight and fetal length which mediated by increased expression of TLR-2, TLR-4, TNF-α, IL-10 and caspase-3 in the placenta

MEKANISME KEMOPREVENTIF EKSTRAK HEKSAN BIJI JINTEN HITAM (Nigella sativa Lor) PADA TIKUS Sprague Dawley DIINDUKSI 7,12 DIMETHYLBENZ(A)ANTRACENE: KAJIAN ANTIOKSIDAN DAN IMUNOMODULATOR

It has been well documented that 7,12-dimethylbenz(a)antracene (DMBA) is a genotoxic carcinogenic able to suppress hematopoiesis and serves as immunosuppressant. Black cumin seed (BJH) has been empirically used as immunostimulant. How chemopreventive mechanisms of hexane extraction of black cumin seeds (EHBJH) on DMBA-induced rats has been unclear yet. The aim of the study was to investigate the effects of EHBJH on activity and mechanism chemopreventive in DMBA-induced rats. The study was conducted on 200 SD rats of 4-5 weeks, divided into 8 group