Detailing the phonetic environment: a sociophonetic study of the London-Bengali community

The present study investigated variability in the production of English by London Bengali adults who speak the Sylheti dialect of Bengali. Speakers had been resident in London for differing lengths of time. They were recorded producing /l/, /r/ and the eleven monophthongal vowels in English. Phonetically trained listeners rated speakers' productions of /l/ and /r/ in terms of manner and place. F1 and F2 formant frequency values, and duration were measured for all monophthongal vowels. The results demonstrated that older speakers (first-generation immigrants) tended to use Sylhetilike variants when speaking English, whilst second-generation speakers tended to use native English-like variants. These findings will be used to inform studies of the role of phonetic input in child language acquisition from the London Bengali community

Targeted Gene Mutations in the Forest Pathogen Dothistroma septosporum Using CRISPR/Cas9.

Dothistroma needle blight, caused by Dothistroma septosporum, has increased in incidence and severity over the last few decades and is now one of the most important global diseases of pines. Disease resistance breeding could be accelerated by knowledge of pathogen virulence factors and their host targets. However, this is hindered due to inefficient targeted gene disruption in D. septosporum, which is required for virulence gene characterisation. Here we report the first successful application of CRISPR/Cas9 gene editing to a Dothideomycete forest pathogen, D. septosporum. Disruption of the dothistromin pathway regulator gene AflR, with a known phenotype, was performed using nonhomologous end-joining repair with an efficiency of > 90%. Transformants with a range of disruption mutations in AflR were produced. Disruption of Ds74283, a D. septosporum gene encoding a secreted cell death elicitor, was also achieved using CRISPR/Cas9, by using a specific donor DNA repair template to aid selection where the phenotype was unknown. In this case, 100% of screened transformants were identified as disruptants. In establishing CRISPR/Cas9 as a tool for gene editing in D. septosporum, our research could fast track the functional characterisation of candidate virulence factors in D. septosporum and helps set the foundation for development of this technology in other forest pathogens.Published onlin

Translating the complexities of flood risk science using KEEPER - a knowledge exchange exploratory tool for professionals in emergency response

Within flood risk management (FRM) decision making, there is a growing interest in participatory approaches to engage and integrate stakeholder expertise. Decision support tools are becoming common features in the FRM ‘toolkit’, yet there is a limited application of participatory methodologies in the construction of such tools. This paper reports on completed FRMRC research (Flood Risk Management Research Consortium, UK http://www.floodrisk.org.uk/) and the construction of a geographic information system-based flood risk assessment tool, KEEPER – a Knowledge Exchange Exploratory tool for Professionals in Emergency Response. An iterative methodology was used to engage emergency professionals throughout the research process, allowing a mixing of scientific and professional expertise in the co-production of KEEPER. KEEPER was both instrumental in facilitating participation and knowledge exchange, and informing recommendations for future tools in practice. This paper argues that participation is both essential for supporting pragmatic flood research and as a means of enhancing communication across traditionally divided communities